MARCH2 (NM_016496) Human Untagged Clone

CAT#: SC114251

41335 (untagged)-Human membrane-associated ring finger (C3HC4) 2 (MARCH2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MARCH2
• Synonyms: HSPC240; MARCH-II; RNF172
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114251 sequence for NM_016496 edited (data generated by NextGen Sequencing)
  ATGACGACGGGTGACTGCTGCCACCTCCCCGGCTCCCTGTGTGACTGCTCCGGCAGCCCT
  GCCTTCTCCAAGGTCGTGGAGGCTACGGGCCTCGGACCGCCCCAGTATGTGGCACAGGTG
  ACTTCAAGGGATGGCCGGCTCCTCTCCACCGTCATCCGTGCCTTGGACACACCGAGTGAT
  GGTCCTTTCTGCCGGATCTGCCATGAGGGAGCGAACGGGGAGTGCTTGCTGTCCCCGTGT
  GGCTGCACCGGCACGCTGGGTGCCGTGCATAAGAGCTGTCTGGAGAAGTGGCTTTCCTCA
  TCTAACACCAGCTACTGCGAGCTGTGCCACACGGAGTTTGCAGTGGAGAAACGGCCTCGA
  CCCCTCACAGAGTGGCTGAAGGACCCGGGGCCGCGGACGGAGAAGCGGACACTGTGCTGC
  GACATGGTGTGTTTCCTGTTCATCACACCGCTGGCCGCCATCTCAGGCTGGTTGTGCCTG
  CGCGGGGCCCAGGACCACCTCCGGCTCCACAGCCAGCTGGAGGCCGTGGGTCTCATTGCC
  CTCACCATCGCCCTCTTCACCATCTATGTCCTCTGGACGCTGGTCTCCTTCCGCTACCAC
  TGCCAGCTGTACTCCGAGTGGAGAAAGACCAACCAGAAAGTTCGCCTGAAGATCCGGGAG
  GCGGACAGCCCCGAGGGCCCCCAGCATTCTCCACTGGCAGCTGGACTCCTGAAGAAGGTG
  GCAGAGGAGACACCAGTATGA
  
  Clone variation with respect to NM_016496.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016496 unedited
  AGAGTTAGAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCCGGCCG
  GAGCGGAGCTAGTGGCGCCGACGGGCCGGGCCGGGCCGGGACCGGGGCCGAGGCGAACCG
  AGGGGCCTGTGCGGCCGCCCGGCCCGGCCGCGGATCAGGCTCCTGGAACCATGGGCCTCA
  GGCCCTGAGGATACGGGGCTCCCGGTGGCCATGACGACGGGTGACTGCTGCCACCTCCCC
  GGCTCCCTGTGTGACTGCTCCGGCAGCCCTGCCTTCTCCAAGGTCGTGGAGGCTACGGGC
  CTCGGACCGCCCCAGTATGTGGCACAGGTGACTTCAAGGGATGGCCGGCTCCTCTCCACC
  GTCATCCGTGCCTTGGACACACCGAGTGATGGTCCTTTCTGCCGGATCTGCCATGAGGGA
  GCGAACGGGGAGTGCTTGCTGTCCCCGTGTGGCTGCACCGGCACGCTGGGTGCCGTGCAT
  AAGAGCTGTCTGGAGAAGTGGCTTTCCTCATCTAACACCAGCTACTGCGAGCTGTGCCAC
  ACGGAGTTTGCAGTGGAGAAACGGCCTCGACCCCTCACAGAGTGGCTGAAGGACCCGGGG
  CCGCGGACGGAGAAGCGGACACTGTGCTGCGACATGGTGTGTTTCCTGTTCATCACACCG
  CTGGCCGCCATCTCAGGCTGGTTGTGCCTGCGCGGGGCCCAGGACCACCTCCGGCTCCAC
  AGCCAGCTGGAGGCCGTGGGTCTCATTGCCCTCACCATCGCCCTCTTCACCATCTATGTC
  CTCTGGACGCTGGTCTCCTTCCGCTACCACTGCCAGCTGTACTCCGAGTGGAGAAAGACC
  AACCAGAAAGTTCGCCTGAAGATCCGGNAGGCGGACAGCCCCGAGGGCCCCCAGCATTCT
  NCACTGGCAGCTGGACTCCTGAAGAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016496 unedited
  CCGCGGGCCGCAATCTAGCATCGAGTTTTTTTTTTTTTTTTTTTTGCCCAGCCAGGGCTT
  CTTTTATTCATTCAGGGGTGGGTTTTTGAAACGCAGTGCAACTTTTATATAAAGCCGAGG
  GTGCCAGGAAAGTGGGCCTGCCGGGTGCAGAGAAGCGCAAGAAGCTTGCTGGAATGTTCC
  AGCTTTAAGGGCGCCCTTGATACACCGGCCACAGCTGCTGGCCCAGAAGGTGCCATGTGG
  ACCTTAGAATGAGATCAGGGTGCCTGCTACGTCCAGAGCTCAGGACCATGTCACACACTG
  TCCCTCCCATATGCCCAACAAAACCCCATCCTACCCCCCCCCCCCCCCCCCCCCCCTCCC
  CCCTACCCCCCCCCCTTCCCCTTTCTCCGTCTCCCCCCCCCCCCTCCCCCCTCTTCGTCC
  CCTCCTTCTCCTCTCCCCCCCCCCTCCCCCCTTCCCCCCCCCTCCCCCCCCCCTCCTCTC
  CTTTCCCCCCCTCCCCTCTCCTTCCCCTTACCGCTTCCCCTCCCTCTCCTCCCCCCCTCA
  CTTCCCCCCTTTCCCCCCCCCCTCCTCTTACCCGCCCGCCTTCCCCCCTTTTCCCATCCC
  CCCCCCCCTTACCTCTCCCCCGCTCCATTCCCCCTCCTCCCCTCCCCCCCCCCCATCCCC
  CTTCCCCCTTCTTCCCTCCCACTCTCCCCCTCCCCTTTCCCCATTCCCCATTCCTTTCTT
  TCCCCCTTTTCCTCTTCCCCCCCTTCCTCCTCCCCCCGCCGCCCCTGTCCCCGCCGTTTC
  TTCCCCCTCCTTTCCTCCCCATCCTCTTCGTCCGCCCCCTCCCCCTCCCCCCCCCCCCTT
  CCCCCCCCCCTTCTCTCTTCTCCCCCGCCGTTATTTGCACACCATCACCCCCTTCCCCCC
  CCCCCCCCTCTCCCTTTCCTGTCCCGTCCACCCT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_016496
• ORF Size: 741 bp
• Insert Size: 1250
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_016496.3, NP_057580.2
• RefSeq Size: 1745
• RefSeq ORF: 741
• Locus ID: 51257
• Domains: RING
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: MARCH2 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH2 reduces surface accumulation of several glycoproteins and appears to regulate early endosome-to-trans-Golgi network (TGN) trafficking (Bartee et al., 2004 [PubMed 14722266]; Nakamura et al., 2005 [PubMed 15689499]). [supplied by OMIM, Mar 2010]
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 4. Variants 1, 2, and 4-7 all encode the longer isoform (1).