CARD8 (NM_014959) Human Untagged Clone

CAT#: SC114692

CARD8 (untagged)-Human caspase recruitment domain family, member 8 (CARD8), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CARD8
• Synonyms: CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014959 edited
  GAATTCGGCACGAGGACAGAACACAGAGCAGCCTGGCAGTGTCCAAGCAACAAGCCTCCG
  CTCCTCCTTCCTGCACCCTGGGGCTCCTGAAACTCACATGAGAAGGAGGGCTGTCTGAGA
  TTCGAGGGAAACAAGCTCTCAGGACTTCCGGTCGCCATGATGGCTGTGGGCGGTAAACGC
  GGTTAGTGCAAGCATCTGGGCCATCTTCAATGGTAAAAAAGATACAGTAAAGACATAAAT
  ACCACATTTGACAAATGGAAAAAAAGGAGTGTCCAGAAAAGAGTAGCAGCAGTGAGGAAG
  AGCTGCCGAGACGGGTATACAGGGAGCTACCCTGTGTTTCTGAGACCCTCTGTGACATCT
  CACATTTTTTCCAAGAAGATGATGAGACAGAGGCAGAGCCATTATTGTTCCGTGCTGTTC
  CTGAGTGTCAACTATCTGGGGGGGACATTCCCAGGAGACATTTGCTCAGAAGAGAATCAA
  ATAGTTTCCTCTTATGCTTCTAAAAAGTCTGTTTTGAGATCGAAGAAGATTATAAAAATC
  GTCAGTTTCTGGGGCCTGAAGGAAATGTGGATGTTGAGTTGATTGATAAGAGCACAAACA
  GATACAGCGTTTGGTTCCCCACTGCTGGCTGGTATCTGTGGTCAGCCACAGGCCTCGGCT
  TCCTGGTAAGGGATGAGGTCACAGTGACGATTGCGTTTGGTTCCTGGAGTCAGCACCTGG
  CCCTGGACCTGCAGCACCATGAACAGTGGCTGGTGGGCGGCCCCTTGTTTGATGTCACTG
  CAGAGCCAGAGGAGGCTGTCGCCGAAATCCACCTCCCCCACTTCATCTCCCTCCAAGCAG
  GTGAGGTGGACGTCTCCTGGTTTCTCGTTGCCCATTTTAAGAATGAAGGGATGGTCCTGG
  AGCATCCAGCCCGGGTGGAGCCTTTCTATGCTGTCCTGGAAAGCCCCAGCTTCTCTCTGA
  TGGGCATCCTGCTGCGGATCGCCAGTGGGACTCGCCTCTCCATCCCCATCACTTCCAACA
  CATTGATCTATTATCACCCCCACCCCGAAGATATTAAGTTCCACTTGTACCTTGTCCCCA
  GCGACGCCTTGCTAACAAAGGCGATAGATGATGAGGAAGATCGCTTCCATGGTGTGCGCC
  TGCAGACTTCGCCCCCAATGGAACCCCTGAACTTTGGTTCCAGTTATATTGTGTCTAATT
  CTGCTAACCTGAAAGTAATGCCCAAGTGGATCTCCAGCTTGTAGCTGCATCAGCCCCTCC
  TCCTTTCTCAGGTGCAGCCTTTGTGAAGGAGAACCACCGGCAACTCCAAGCCAGGATGGG
  GGACCTGAAAGGGGTGCTCGATGATCTCCAGGACAATGAGGTTCTTACTGAGAATGAGAA
  GGAGCTGGTGGAGCAGGAAAAGACACGGCAGAGCAAGAATGAGGCCTTGCTGAGCATGGT
  GGAGAAGAAAGGGGACCTGGCCCTGGACGTGCTCTTCAGAAGCATTAGTGAAAGGGACCC
  TTACCTCGTGTCCTATCTTAGACAGCAGAATTTGTAAAATGAGTCAGTTAGGTAGTCTGG
  AAGAGAGAATCCAGCGTTCTCATTGGAAATGGATAAACAGAAATGTGATCATTGATTTCA
  GTGTTCAAGACAGAAGAAGACTGGGTAACATCTATCACACAGGCTTTCAGGACAGACTTG
  TAACCTGGCATGTACCTATTGACTGTATCCTCATGCATTTTCCTCAAGAATGTCTGAAGA
  AGGTAGTAATATTCCTTTTAAATTTTTTCCAACCATTGCTTGATATATCACTATTTTATC
  CATTGACATGATTCTTGAAGACCCAGGATAAAGGACATCCGGATAGGTGTGTTTATGAAG
  GATGGGGCCTGGAAAGGCAACTTTTCCTGATTAATGTGAAAAATAATTCCTATGGACACT
  CCGTTTGAAGTATCACCTTCTCATAACTAAAAGCAGAAAAGCTAACAAAAGCTTCTCAGC
  TGAGGACACTCAAGGCATACATGATGACAGTCTTTTTTTTTTTTTTGTATGTTAGGACTT
  TAACACTTTATCTATGGCTACTGTTATTAGAACAATGTAAATGTATTTGCTGAAAGAGAG
  CACAAAAATGGGAGAAAATGCAAACATGAGCAGAAAATATTTTCCCACTGGTGTGTAGCC
  TGCTACAAGGAGTTGTTGGGTTAAATGTTCATGGTCAACTCCAAGGAATACTGAGATGAA
  ATGTGGTAAATCAACTCCACAGAACCACCAAAAAGAAAATGAGGGTAATTCAGCTTATTC
  TGAGACAGACATTCCTGGCAATGTACCATACAAAAAATAAGCCAACTCTGACATTTGGAT
  TCTACCATAGACTCTGTCATTTTGTAGCCATTTCAGCTGTCTTTTGATTAATGTTTTCGT
  GGCACACATATTTCCATCCTTTTATGTTTAATCTGTTTAAAACAAGTTCCTAGTAGAAAA
  AAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014959 unedited
  ACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGACAGAACACANAGCAGCCTGGC
  AGTGTCCAAGCAACAAGCCTCCGCTCCTCCTTCCTGCACCCTGGGNNGCTCCTGAAACTC
  ACATGAGAAGGAGGGCTGTCTGAGATTCGAGGGAAACAAGCTCTCAGGACTTCCGGTCGC
  CATGATGGCTGTGGGCGGTAAACGCGGTTAGTGCAAGCATCTGGGCCATCTTCAATGGTA
  AAAAAGATACAGTAAAGACATAAATACCACATTTGACAAATGGAAAAAAAGGAGTGTCCA
  GAAAAGAGTAGCAGCAGTGAGGAAGAGCTGCCGAGACGGGTATACAGGGAGCTACCCTGT
  GTTTCTGAGACCCTCTGTGACATCTCACATTTTTTCCAAGAAGATGATGAGACAGAGGCA
  GAGCCATTATTGTTCCGTGCTGTTCCTGAGTGTCAACTATCTGGGGGGGACATTCCCAGG
  AGACATTTGCTCAGAAGAGAATCAAATAGTTTCCTCTTATGCTTCTAAAAAGTCTGTTTT
  GAGATCGAAGAAGATTATAAAAATCGTCAGTTTCTGGGGCCTGAAGGAAATGTGGATGTT
  GAGTTGATTGATAAGAGCACAAACAGATACAGCGTTTGGTTCCCCACTGCTGGCTGGTAT
  CTGTGGTCAGCCACAGGCCTCGGCTTCCTGGAAGGGATGAGGTCACAGTGACGATTGCGT
  TTGGTTCCTGGAGTCAGCACCTGGCCCTGGACCTGCAGCACCATGAACAGTGGCTGGTGG
  GCGGCCCCTTNGTTGATGTCACTGCAGAGCCAGAGGAGGCTGTCGNCGAAATCCACTCCC
  CCATTCATCTCCCCTCAGCAGTGAGGTGGACGTCTCCTGTTTNNCTCGTGCCCATTNTAG
  AATGAAGGNNATGTCCTGNAGCATNCANCCGGNTGGAGCCTTCTATGCTGTCCTGAAGNC
  CCAGCTCTTCTGATGGCATCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014959 unedited
  CGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTNTCTACTAGGAAACTTGNTT
  TTAAACAGNNATTAAACATTAAAAGGGAGTGGAAATATGTGGTGCCACGAAAAACATTAA
  TCAAAAGACAGCTGAAATGGCTACAAAATGACAGAGTCTATGGTAGAATCCAAATGTCAG
  AGTTGGCTTATTTTTTGTATGGTACATTGCCAGGAATGTCTGTCTCAAAATAAGCTGAAT
  TACCCTCATTTTCTTTTTGGTGGTTCTGTGGAGTTGATTTACCACATTTCATCTCAGTAT
  TCCTTGGAGTTGACCATGAACATTTAACCCAACAACTCCTTGTAGCAGGCTACACACCAG
  TGGGAAAATATTTTCTGCTCATGTTTGCATTTTCTCCCATTTTTGTGCTCTCTTTCAGCA
  AATACATTTACATTGTTCTAATAACAGTAGCCATAGATAAAGTGTTAAAGTCCTAACATA
  CAAAAAAAAAAAAAAGACTGTCATCATGTATGCCTTGAGTGTCCTCAGCTGAGAAGCTTT
  TGTTAGCTTTTCTGCTTTTAGTTATGAGAAGGTGATACTTCAAACGGAGTGTCCATAGGA
  ATTATTTTTCACATTAATCAGGAAAAGTTGCCTTTCCAGGCCCCATCCTTCATAAACACA
  CCTATCCGGATGTCCTTTATCCTGGGTCTTTAAGAATCATGTCAATGGATAAAATAGTGA
  TATATCAAGCCATGGGTTGGAAAAAATTAAAAGGAATATTACTACCTTCTTCAAACTTTC
  TTGAGGAAAATGCTGAAGATACAGTCAATAGGTACTGCCACGTTACAAGGTCTGCCTGAA
  GCCTGGGTGAAAGAATGTACCCACATCTTTTCTGCCTTGACACTGAAATCAAGAACACAT
  TTCTGGTTTTCCATTCCATGGAAACGCTGGATCTTTTTTCAGACTACTAACGACTCTTTT
  ACAATTCGGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014959
• ORF Size: 1296 bp
• Insert Size: 2620
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_014959.1, NP_055774.1
• RefSeq Size: 5059
• RefSeq ORF: 1296
• Locus ID: 22900
• Domains: CARD
• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway
• Gene Summary: The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform b), compared to isoform a. Variants 2, 3 and 11 encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.