Josephin 1 (JOSD1) (NM_014876) Human Untagged Clone

CAT#: SC114727

JOSD1 (untagged)-Human Josephin domain containing 1 (JOSD1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: JOSD1
• Synonyms: dJ508I15.2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114727 sequence for NM_014876 edited (data generated by NextGen Sequencing)
  ATGAGTTGTGTGCCATGGAAAGGAGACAAGGCCAAATCTGAATCATTGGAGCTGCCCCAG
  GCAGCACCCCCACAAATCTACCATGAGAAACAGCGCAGGGAGCTTTGTGCCCTCCACGCC
  CTCAATAACGTCTTCCAGGACAGCAATGCCTTCACCCGGGATACGCTGCAAGAGATTTTC
  CAGAGGTTGTCTCCAAACACCATGGTGACACCTCACAAGAAGAGCATGCTGGGAAATGGC
  AACTACGATGTGAATGTCATTATGGCAGCACTTCAGACCAAAGGCTATGAAGCTGTTTGG
  TGGGACAAGCGCAGGGATGTCGGTGTCATTGCCCTCACTAACGTCATGGGCTTCATCATG
  AATCTGCCCTCCAGCCTATGCTGGGGTCCACTGAAACTGCCCCTCAAAAGGCAGCACTGG
  ATCTGTGTTCGAGAGGTGGGAGGGGCCTACTACAACCTCGACTCCAAACTCAAGATGCCC
  GAGTGGATTGGAGGCGAGAGCGAGCTCAGGAAGTTTCTAAAACATCATTTGCGAGGAAAG
  AACTGTGAACTCCTGCTGGTGGTACCAGAAGAGGTAGAGGCTCATCAGAGTTGGAGGACC
  GATGTGTAA
  
  Clone variation with respect to NM_014876.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014876 unedited
  GGGTTCAGAAATTATGTATACGACTCACTATAGGGCGGCCCGCGAATCGGCACGAGGCTT
  ACATTTTATGTGTTTTTTTAAATTCTGCACTTTGACAGTGGTAGCAAAATAAAAGTAATC
  CAAAAGTTATTTTTCTATTGAGAAGAGAGAAGAGGTGGGTTTTATTTTTTTCCTTTTGAT
  TCCAATCTTTTCTCCCAGTGACAAAGCATAAATCATGGACACCAGAGAATAAGGTGGACC
  TCAGGAATCCGGAAAAGACTGAGAAGCTTACATTCTTCCTCTAGAGGGAAGAGTGGGGTG
  TTTATAGCCATCTCTGGAACCTAAAACAAAAAACATGAGTTGTGTGCCATGGAAAGGAGA
  CAAGGCCAAATCTGAATCATTGGAGCTGCCCCAGGCAGCACCCCCACAAATCTACCATGA
  GAAACAGCGCAGGGAGCTTTGTGCCCTCCACGCCCTCAATAACGTCTTCCAGGACAGCAA
  TGCCTTCACCCGGGATACGCTGCAAGAGATTTTCCAGAGGTTGTCTCCAAACACCATGGT
  GACACCTCACAAGAAGAGCATGCTGGGAAATGGCAACTACGATGTGAATGTCATTATGGC
  AGCACTTCAGACCAAAGGCTATGAAGCTGTTTGGTGGGACAAGCGCAGGGATGTCGGTGT
  CATTGCCCTCACTAACGTCATGGGCTTCATCATGAATCTGCCCTCCAGCCTATGCTGGGG
  TTCACTGGAACTGCCCCTCAAAAGCAGCACTGGATCTGTTGTCGAGAGGTGGGGAGGGCC
  TACTACACCTCGACTCCAACTCAAGTGCCCGATGGATTGGAGCGAGAGCACCTCAGAAGT
  CTAAACATATTGCGAGGAAGACTGTGACTCTGCTGTGGACCAGAGAGTANAGCTATCACA
  GTTGAGACCGTGGTACAGTCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014876 unedited
  GGCTCCGGGCCACCGTCGAGTATCGAGTTAATCCTTTTTTTTTTTGGAGTTTTCCATTTT
  TAATATTTGGTTAAACAAAATACATCTTTTGTAAACAAACGCAGCACAAGGCCAACAACA
  AAAAGGAATAAAAGCAAGAAAAACAAAAAGCCTAAGGTTGCCCGTTATTGGGCATCAGGG
  TCAGGCCTGTTCACTTCCTTAAAGGAAGTGGGGCTTTTTGCCCTTGGGCATCAGCATGGG
  TCCCTGTTCCTAAGCACCAGAATTGGATATGAACAGAGAAACCAGCCCTGAAATGTTTAA
  GCGTCTTTGCATATCCCATTGGCTCATGGACTCCAAGTGCATGGGGACTCTTTCAAGTGC
  CATTTGCCCAGGCCCAAGTGGATTTGTGGAGAGGAGTTTCCTCCTCTACCCAAGAAATGG
  CTTACTATCTGACCGTGCCAGTGGCCCCATGGTCCTGCTTGTGATCTTCAGCTAGGGTGG
  CTGCTTCGCCTGGCTGGCGGCGGTCGGATTGTCCTTGTGCTGGGCTCGATAAGAGCTCCC
  TGTGGCGCCCTGCTGGCCTGGTTGTTTTTTTGGTGCCTCTTATACTGCCGCCTTTCGCTT
  GTCTTCTTTTCCGCCTCCTCCCTCTGGGGCCTGTTCCTCCTTTCTTCCCTCCCGCCTTGT
  GGGCCTGGTGCCCCCGCTGCCTGTTTTGGCCTGGGCTTGCCCGTCTTTCTTCTCTTTGTT
  GCTCTTTTTCTTTCGCTCCTCGGCTTTCTCTGCTTCTCTCTTTTCTTTTCTCCTTCTTCT
  CTCTTCTCTTTCTCTCCCCTCCTCTCTCTCTCTTCTCCTGTTCTCGTCTTGGCCTTTTTT
  CCTCTCCTCTTCTGCCCTCTTCGGCTCCTCTCTCGTTTCCGCTTTCCCCTCCTCCCCCGT
  CCTCCGCCTTCTCGCTTCCCTCTCCCCATCGCTCTCTGCTTCGCCCCCCTCTTTCTCGTC
  NTCTCTTCTCTCGTTCCCCCTCCCCTCTTGGCTCCCCGCGTTCGCTACGCACTACAACAC
  AAACACAAACAAGAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014876
• ORF Size: 609 bp
• Insert Size: 3240
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_014876.3, NP_055691.1
• RefSeq Size: 3207
• RefSeq ORF: 609
• Locus ID: 9929
• Domains: Josephin
• Protein Families: Druggable Genome
• Gene Summary: Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity. [UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the predominant transcript. Variants 1, 2, and 3 encode the same protein.