DDX46 (NM_014829) Human Untagged Clone

CAT#: SC114794

DDX46 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46)


  "NM_014829" in other vectors (5)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol DDX46
Synonyms Prp5; PRPF5
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_014829, the custom clone sequence may differ by one or more nucleotides


ATGGGTCGGGAGTCACGCCACTATCGAAAACGATCGGCATCCCGGGGTCGCTCTGGAAGTCGGTCTAGAA
GTCGCTCACCCTCAGACAAAAGAAGTAAACGTGGAGATGACAGACGGTCTAGAAGTAGAGATAGAGATAG
GAGGAGAGAGAGGTCTCGTAGCAGGGATAAAAGAAGATCTCGGTCAAGGGACAGGAAGCGTCTGAGACGT
TCCAGAAGTAGAGAGAGAGACAGAAGCCGAGAGCGAAGAAGATCTCGAAGTAGAGACAGGAGACGCTCAA
GGAGTAGAAGCCGGGGCCGGCGATCCCGATCCTCCAGTCCTGGAAATAAAAGCAAGAAAACTGAGAATAG
ATCTAGGTCCAAAGAGAAAACTGATGGTGGGGAAAGTTCTAAAGAGAAGAAAAAAGACAAAGATGACAAG
GAGGATGAAAAAGAAAAAGATGCTGGCAACTTTGACCAGAATAAGCTGGAAGAAGAAATGAGAAAGCGAA
AAGAAAGAGTAGAAAAATGGCGAGAAGAGCAACGTAAAAAGGCTATGGAAAACATAGGAGAACTGAAAAA
GGAAATCGAAGAGATGAAACAAGGGAAAAAGTGGAGTTTAGAGGACGATGATGATGACGAAGATGATCCT
GCAGAAGCTGAAAAGGAGGGAAATGAAATGGAGGGTGAGGAGTTAGATCCATTAGATGCTTACATGGAAG
AAGTGAAAGAGGAAGTAAAAAAATTTAACATGAGAAGTGTAAAAGGTGGTGGGGGAAATGAAAAGAAGTC
TGGGCCAACGGTCACAAAAGTTGTCACTGTTGTGACAACCAAAAAAGCAGTTGTGGATTCTGATAAGAAG
AAAGGTGAGCTGATGGAGAATGACCAGGATGCCATGGAGTATTCTTCAGAGGAGGAAGAAGTTGATCTTC
AGACAGCCCTTACAGGGTATCAAACAAAACAGCGAAAGCTTCTAGAACCAGTTGATCATGGAAAAATTGA
GTATGAGCCATTTAGGAAAAACTTCTATGTTGAAGTTCCAGAACTAGCAAAAATGTCTCAAGAAGAGGTA
AATGTGTTTCGATTGGAAATGGAGGGCATTACAGTTAAAGGAAAAGGTTGCCCCAAACCAATTAAATCCT
GGGTCCAGTGTGGAATTTCCATGAAGATCTTAAATTCCCTCAAGAAGCATGGCTATGAAAAGCCCACGCC
CATCCAAACCCAAGCTATTCCTGCTATAATGTCTGGACGAGATTTGATTGGCATTGCCAAAACAGGAAGT
GGAAAGACCATTGCTTTTCTGTTGCCCATGTTTAGACACATCATGGATCAGAGGTCATTAGAGGAAGGAG
AGGGGCCAATAGCTGTCATCATGACTCCAACTCGAGAACTGGCTTTACAGATTACTAAAGAGTGTAAGAA
GTTTTCCAAGACTTTGGGACTTAGAGTGGTCTGTGTTTACGGAGGAACAGGAATCAGTGAGCAGATTGCT
GAGCTGAAAAGAGGTGCTGAAATTATTGTTTGCACACCTGGTCGAATGATTGACATGTTAGCCGCTAACA
GTGGTCGGGTCACAAATCTTCGAAGAGTGACATATGTTGTTTTAGATGAAGCAGACAGAATGTTTGACAT
GGGTTTTGAACCCCAGGTCATGCGCATCGTGGATAATGTTCGTCCTGATCGACAGACGGTTATGTTTTCA
GCTACTTTCCCCAGAGCTATGGAGGCTTTGGCTCGCAGGATCCTCAGTAAACCTATTGAAGTACAAGTTG
GAGGCAGGAGTGTGGTTTGCTCAGATGTGGAGCAACAAGTGATTGTGATTGAAGAAGAAAAGAAATTCTT
GAAGTTACTTGAGCTTCTAGGCCATTATCAAGAGTCAGGATCTGTCATTATATTTGTGGATAAGCAGGAA
CATGCTGATGGTCTTCTTAAGGATTTAATGAGAGCATCTTATCCTTGCATGTCTCTTCATGGAGGCATTG
ATCAATATGACAGAGATAGCATCATAAATGACTTTAAGAATGGGACCTGCAAACTTCTTGTGGCTACCTC
TGTTGCTGCCCGAGGTCTAGATGTGAAACATCTGATTCTTGTAGTAAATTATAGCTGCCCCAACCATTAT
GAGGATTATGTACACAGAGCAGGGCGGACTGGAAGAGCAGGAAACAAGGGTTATGCTTATACTTTTATCA
CAGAGGATCAAGCTCGCTATGCTGGTGACATAATTAAAGCTCTTGAATTGTCAGGGACTGCAGTACCTCC
TGATTTAGAGAAACTGTGGAGTGATTTCAAAGATCAGCAGAAAGCTGAGGGGAAAATAATTAAAAAGAGT
AGTGGGTTCTCTGGTAAGGGATTCAAGTTTGATGAAACAGAACAAGCTTTGGCTAATGAGAGGAAGAAGT
TACAAAAAGCAGCTCTTGGTCTACAAGATTCAGATGATGAGGATGCTGCAGTTGATATTGATGAGCAAAT
TGAAAGCATGTTTAATTCAAAGAAGAGAGTAAAGGATATGGCTGCTCCTGGAACATCAAGTGTTCCTGCT
CCAACTGCAGGAAATGCTGAGAAATTAGAAATTGCTAAGAGATTGGCTCTTAGAATCAATGCCCAGAAGA
ATTTGGGCATCGAGTCTCAGGATGTGATGCAGCAGGCCACCAATGCAATTCTTAGGGGTGGCACCATTCT
GGCTCCCACTGTTTCTGCAAAAACCATTGCAGAACAACTTGCTGAAAAGATCAATGCCAAGCTCAATTAT
GTGCCGTTAGAGAAACAAGAAGAAGAGAGACAGGATGGTGGACAGAATGAATCTTTTAAGAGATATGAAG
AAGAATTAGAGATCAATGACTTCCCACAGACTGCTAGGTGGAAAGTTACCTCTAAGGAAGCTCTGCAGAG
AATCAGTGAATACTCTGAAGCCGCAATTACAATCAGAGGAACCTACTTCCCTCCTGGCAAAGAACCCAAG
GAAGGCGAGCGGAAGATTTACTTGGCAATTGAAAGTGCCAATGAACTGGCTGTGCAGAAAGCAAAGGCAG
AAATCACCAGGCTCATAAAAGAAGAGCTGATCCGGCTGCAAAATTCATACCAACCAACAAATAAAGGAAG
ATACAAAGTCTTATAG


>OriGene 5' read for NM_014829 unedited
TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGGGATGGCCGCCAC
AGCTGTAGGTGCTGCTAGTGTTTAGCGCTGGTCTTTGCCGGGCGTTGAGGGCAGCTCAGC
CTCCTTGTTTGTCCGGTTCGCCTGTGCGTGGTACTCAAGGGCACCAGTATTCCCGCGGTC
GGCAGCATGGGTCGGGAGTCACGCCACTATCGAAAACGATCGGCATCCCGGGGTCGCTCT
GGAAGTCGGTCTAGAAGTCGCTCACCCTCAGACAAAAGAAGTAAACGTGGAGATGACAGA
CGGTCTAGAAGTAGAGATAGAGATAGGAGGAGAGGAGAGGTCTCGTAGCAGGGATAAAAG
AAGATCTCGGTCAAGGGACAGGAAGCGTCTGAGACGTTCCAGAAGTAGAGAGAGAGACAG
AAGCCGAGAGCGAAGAAGATCTCGAAGTAGAGACAGGAGACGCTCAAGGGAGTAGAAGCC
GGGGGCCGGCGATCCCGATCCTCCAGTCCTGGAAATAAAAGCAAGAAAACTGAGAATAGA
TTCTAGGTCCAAAGAGAAAACTGATGGTGGNGGGAAAGNNTCTAAGAGAAGAANAAAGAA
CAAGATGACAAGGAGGATGAAAAAGAANAAGATGCTGGCAACTTTGACCAGAATAAGCTG
GGAAGAAGAAATGAGAAAGCGAANAGAAAGAGTAGAAAAATGGCGAAGAGAGCAACGTAN
AAAGGCTATGGAAAACATAGGAGAACTGAAAAAGGAAATCGAAGAGATGAACANGGNAAN
AAGGGNAGGTTAAAGGACGATGATGATGACAAAAATGATCCTGCAGAAGCTGAAAAGGAA
GGAAAGAAATGGAGGGTGAGGAGTTAAATCCCTTAAATGCTTACTGGAAAAAGGAAAGAG
GAATTAAAAATTTTACTGAAAAGTGTAAAGGGGGGGGGGGAAATGAAAAAAATTCTGCCA
>OriGene 3' read for NM_014829 unedited
ACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCTTTTGTAAGACTTC
TATTATAACATTCTTAAATCCAGTTCATATTGTTCCAGAGTCATGTAACATCAAGGTGGG
GACACAAAATATGATAGTGAATTTTACCTTTTCTTAGGAAATGATCACAATTTCATTGAA
AGTACAGGATAAAGTGAATAGCCCCTTAACGACTTGTGTACTTTATTGTCTTCAATAGAA
AGTTCAAATATTTCCATTTTTTAAACCTCTAGAATATTATCCGGACATTTAAAATATTGA
TATTTGAGTTTACATTAAAGGACATCATACTCTGAAAACAGGCTGGCTTTAAATATAGTT
TGACTGATTGTGGGGGTACTTACGGACAGATTTCTTTACCAAGTACCAGTTTCTTATATT
TAAAAAAAATCAGCAAGATAGAATTTAAAAAATCTTCATTTACAATACATTGTAAACTGC
AGACAGCAACTGCCACATACATCATAGACCAGCACAGGTAAAAATCTTTTTTCCGGATGT
CTATAAGACTTTGTATCTTCCTTTATTTGTTGGTTGGTATGAATTTTGCAGCCGGATCAG
CTCTTCTTTTATGAGCCTGGTGATTTCTGCCTTTGCTTTCTGCACAGCCAGTTCATTGGC
ACTTTCAATTGCCAAGTAAATCTTCCGCTCGCCTTCCTTGGGTTCTTTGCCAGGAGGGAA
GTANGTTCCTCTGATTGTAATTGCGGCTTCAGAGTATTCACTGATTCTCTGCAGAGCTTC
TTAGAGGTAACTTTCCCCTAGCAGTCTGTGGGAAGNCATTGATCTCTAAATCTTCTCATA
TCTCTAAAAGATNCATTCTGCCACCATCCTGGCTCTCTTCTTNCTGGTTCTCTACGCACA
TATTGAGCTGGCATTGATCTTTTCAGCAGTGTCTG
Restriction Sites NotI-NotI     
ACCN NM_014829
ORF Size 3096 bp
Insert Size 4100
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Reference Data
RefSeq NM_014829.2, NP_055644.2
RefSeq Size 5695
RefSeq ORF 3096
Locus ID 9879
Domains DEAD, helicase_C
Protein Pathways Spliceosome
Gene Summary This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the 17S U2 snRNP complex; it plays an important role in pre-mRNA splicing. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2014]
Transcript Variant: This variant (2) has an alternate splice site in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (2) lacks an internal aa, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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