DDX46 (NM_014829) Human Untagged Clone

CAT#: SC324257

DDX46 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX46
• Synonyms: Prp5; PRPF5
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014829.2 GGCCGCCACAGCTGTAGGTGCTGCTAGTGTTTAGCGCTGGTCTTTGCCGGGCGTTGAGGG
  CAGCTCAGCCTCCTTGTTTGTCCGGTTCGCCTGTGCGTGGTACTCAAGGGCACCAGTATT
  CCCGCGGTCGGCAGCATGGGTCGGGAGTCACGCCACTATCGAAAACGATCGGCATCCCGG
  GGTCGCTCTGGAAGTCGGTCTAGAAGTCGCTCACCCTCAGACAAAAGAAGTAAACGTGGA
  GATGACAGACGGTCTAGAAGTAGAGATAGAGATAGGAGGAGAGAGAGGTCTCGTAGCAGG
  GATAAAAGAAGATCTCGGTCAAGGGACAGGAAGCGTCTGAGACGTTCCAGAAGTAGAGAG
  AGAGACAGAAGCCGAGAGCGAAGAAGATCTCGAAGTAGAGACAGGAGACGCTCAAGGAGT
  AGAAGCCGGGGCCGGCGATCCCGATCCTCCAGTCCTGGAAATAAAAGCAAGAAAACTGAG
  AATAGATCTAGGTCCAAAGAGAAAACTGATGGTGGGGAAAGTTCTAAAGAGAAGAAAAAA
  GACAAAGATGACAAGGAGGATGAAAAAGAAAAAGATGCTGGCAACTTTGACCAGAATAAG
  CTGGAAGAAGAAATGAGAAAGCGAAAAGAAAGAGTAGAAAAATGGCGAGAAGAGCAACGT
  AAAAAGGCTATGGAAAACATAGGAGAACTGAAAAAGGAAATCGAAGAGATGAAACAAGGG
  AAAAAGTGGAGTTTAGAGGACGATGATGATGACGAAGATGATCCTGCAGAAGCTGAAAAG
  GAGGGAAATGAAATGGAGGGTGAGGAGTTAGATCCATTAGATGCTTACATGGAAGAAGTG
  AAAGAGGAAGTAAAAAAATTTAACATGAGAAGTGTAAAAGGTGGTGGGGGAAATGAAAAG
  AAGTCTGGGCCAACGGTCACAAAAGTTGTCACTGTTGTGACAACCAAAAAAGCAGTTGTG
  GATTCTGATAAGAAGAAAGGTGAGCTGATGGAGAATGACCAGGATGCCATGGAGTATTCT
  TCAGAGGAGGAAGAAGTTGATCTTCAGACAGCCCTTACAGGGTATCAAACAAAACAGCGA
  AAGCTTCTAGAACCAGTTGATCATGGAAAAATTGAGTATGAGCCATTTAGGAAAAACTTC
  TATGTTGAAGTTCCAGAACTAGCAAAAATGTCTCAAGAAGAGGTAAATGTGTTTCGATTG
  GAAATGGAGGGCATTACAGTTAAAGGAAAAGGTTGCCCCAAACCAATTAAATCCTGGGTC
  CAGTGTGGAATTTCCATGAAGATCTTAAATTCCCTCAAGAAGCATGGCTATGAAAAGCCC
  ACGCCCATCCAAACCCAAGCTATTCCTGCTATAATGTCTGGACGAGATTTGATTGGCATT
  GCCAAAACAGGAAGTGGAAAGACCATTGCTTTTCTGTTGCCCATGTTTAGACACATCATG
  GATCAGAGGTCATTAGAGGAAGGAGAGGGGCCAATAGCTGTCATCATGACTCCAACTCGA
  GAACTGGCTTTACAGATTACTAAAGAGTGTAAGAAGTTTTCCAAGACTTTGGGACTTAGA
  GTGGTCTGTGTTTACGGAGGAACAGGAATCAGTGAGCAGATTGCTGAGCTGAAAAGAGGT
  GCTGAAATTATTGTTTGCACACCTGGTCGAATGATTGACATGTTAGCCGCTAACAGTGGT
  CGGGTCACAAATCTTCGAAGAGTGACATATGTTGTTTTAGATGAAGCAGACAGAATGTTT
  GACATGGGTTTTGAACCCCAGGTCATGCGCATCGTGGATAATGTTCGTCCTGATCGACAG
  ACGGTTATGTTTTCAGCTACTTTCCCCAGAGCTATGGAGGCTTTGGCTCGCAGGATCCTC
  AGTAAACCTATTGAAGTACAAGTTGGAGGCAGGAGTGTGGTTTGCTCAGATGTGGAGCAA
  CAAGTGATTGTGATTGAAGAAGAAAAGAAATTCTTGAAGTTACTTGAGCTTCTAGGCCAT
  TATCAAGAGTCAGGATCTGTCATTATATTTGTGGATAAGCAGGAACATGCTGATGGTCTT
  CTTAAGGATTTAATGAGAGCATCTTATCCTTGCATGTCTCTTCATGGAGGCATTGATCAA
  TATGACAGAGATAGCATCATAAATGACTTTAAGAATGGGACCTGCAAACTTCTTGTGGCT
  ACCTCTGTTGCTGCCCGAGGTCTAGATGTGAAACATCTGATTCTTGTAGTAAATTATAGC
  TGCCCCAACCATTATGAGGATTATGTACACAGAGCAGGGCGGACTGGAAGAGCAGGAAAC
  AAGGGTTATGCTTATACTTTTATCACAGAGGATCAAGCTCGCTATGCTGGTGACATAATT
  AAAGCTCTTGAATTGTCAGGGACTGCAGTACCTCCTGATTTAGAGAAACTGTGGAGTGAT
  TTCAAAGATCAGCAGAAAGCTGAGGGGAAAATAATTAAAAAGAGTAGTGGGTTCTCTGGT
  AAGGGATTCAAGTTTGATGAAACAGAACAAGCTTTGGCTAATGAGAGGAAGAAGTTACAA
  AAAGCAGCTCTTGGTCTACAAGATTCAGATGATGAGGATGCTGCAGTTGATATTGATGAG
  CAAATTGAAAGCATGTTTAATTCAAAGAAGAGAGTAAAGGATATGGCTGCTCCTGGAACA
  TCAAGTGTTCCTGCTCCAACTGCAGGAAATGCTGAGAAATTAGAAATTGCTAAGAGATTG
  GCTCTTAGAATCAATGCCCAGAAGAATTTGGGCATCGAGTCTCAGGATGTGATGCAGCAG
  GCCACCAATGCAATTCTTAGGGGTGGCACCATTCTGGCTCCCACTGTTTCTGCAAAAACC
  ATTGCAGAACAACTTGCTGAAAAGATCAATGCCAAGCTCAATTATGTGCCGTTAGAGAAA
  CAAGAAGAAGAGAGACAGGATGGTGGACAGAATGAATCTTTTAAGAGATATGAAGAAGAA
  TTAGAGATCAATGACTTCCCACAGACTGCTAGGTGGAAAGTTACCTCTAAGGAAGCTCTG
  CAGAGAATCAGTGAATACTCTGAAGCCGCAATTACAATCAGAGGAACCTACTTCCCTCCT
  GGCAAAGAACCCAAGGAAGGCGAGCGGAAGATTTACTTGGCAATTGAAAGTGCCAATGAA
  CTGGCTGTGCAGAAAGCAAAGGCAGAAATCACCAGGCTCATAAAAGAAGAGCTGATCCGG
  CTGCAAAATTCATACCAACCAACAAATAAAGGAAGATACAAAGTCTTATAGACATCCGGA
  AAAAAGATTTTTACCTGTGCTGGTCTATGATGTATGTGGCAGTTGCTGTCTGCAGTTTAC
  AATGTATTGTAAATGAAGATTTTTTAAATTCTATCTTGCTGATTTTTTTTAAATATAAGA
  AACTGGTACTTGGTAAAGAAATCTGTCCGTAAGTACCCCCACAATCAGTCAAACTATATT
  TAAAGCCAGCCTGTTTTCAGAGTATGATGTCCTTTAATGTAAACTCAAATATCAATATTT
  TAAATGTCCGGATAATATTCTAGAGGTTTAAAAAATGGAAATATTTGAACTTTCTATTGA
  AGACAATAAAGTACACAAGTCGTTAAGGGGCTATTCACTTTATCCTGTACTTTCAATGAA
  ATTGTGATCATTTCCTAAGAAAAGGTAAAATTCACTATCATATTTTGTGTCCCCACCTTG
  ATGTTACATGACTCTGGAACAATATGAACTGGATTTAAGAATGTTATAATAGAAGTCTTA
  CAAAATGGGTTGAGAGTTTTTGTTTCAATTTTTATCATCATAAATGGGCAAAAAGTAGTT
  GGACTAATTTCGGTTTTTATACAAGTAAAGATTTAATAGTAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014829.2 unedited
  GGCATTGCCCTGTAATGCCCCTCCCGCATGTTTTGACCTCCACTAGAAGACACCGGGACC
  GACTCCAGCCTCCGGACTCTAGCCTAGGCCGCGGGACGGATAACAATCTCACACAGGAAA
  CAGCTATGACCATTAGGCCTATTTAGGTGACACTATAGAACAAGTTTGTACAAAAAAGCA
  GGCTGGTACCGGTCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGCGGACGCGTGG
  GGGCCGCCACAGCTGTAGGTGCTGCTAGTGTTTAGCGCTGGTCTTTGCCGGGCGTTGAGG
  GCAGCTCAGCCTCCTTGTTTGTCCGGTTCGCCTGTGCGTGGTACTCAAGGGCACCAGTAT
  TCCCGCGGTCGGCAGCATGGGTCGGGAGTCACGCCACTATCGAAAACGATCGGCATCCCG
  GGGTCGCTCTGGAAGTCGGTCTAGAAGTCGCTCACCCTCAGACAAAAGAAGTAAACGTGG
  AGATGACAGACGGTCTAGAAGTAGAGATAGAGATAGGAGGAGAGAGAGGTCTCGTAGCAG
  GGATAAAAGAAGATCTCGGTCAAGGGACAGGAAGCGTCTGAGACGTTCCAGAGTAGAGAG
  AGAGACAGAAGCCGAGAGCGAAGAAGATCTCGAAGTAGAGACAGGAGACGCTCAAGGAGT
  AGAAGCCGGGGCCGGCGATCCCGATCCTCCAGTCCTGGAAATAAAAGCAAGAAAACTGAG
  AATAGATCTAGGTCCAAAGAGAAAACTGATGGTGGGGAAAGTTCTAAAGAGAGAAAAAGG
  ACAAAGATGACAGGAGATGAAAAGAAAAGATGCTGGCAACTTTGACCAGAATAGCTGGAG
  AGAAATGAGAAGCGAAAAGAAGAGTAGAAAAAATGGCGAGGAAGAGCACGTAAAACGCTA
  TGAAACATACGAGACTGAAAAGAATCGAAGAGTGAACCAGGGAAAAGTGGGAGTTAAAGA
  CAGATG
• Restriction Sites: Please inquire
• ACCN: NM_014829
• ORF Size: 3096 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_014829.2, NP_055644.2
• RefSeq Size: 5695
• RefSeq ORF: 3096
• Locus ID: 9879
• Domains: DEAD, helicase_C
• Protein Pathways: Spliceosome
• Gene Summary: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the 17S U2 snRNP complex; it plays an important role in pre-mRNA splicing. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (2) has an alternate splice site in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (2) lacks an internal aa, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.