UBF1 (UBTF) (NM_014233) Human Untagged Clone

CAT#: SC115105

UBTF (untagged)-Human upstream binding transcription factor, RNA polymerase I (UBTF), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBTF
• Synonyms: CONDBA; NOR-90; UBF; UBF-1; UBF1; UBF2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_014233 edited
  ATGAACGGAGAAGCCGACTGCCCCACAGACCTGGAAATGGCCGCCCCCAAAGGCCAAGAC
  CGTTGGTCCCAGGAAGACATGCTGACTTTGCTGGAATGCATGAAGAACAACCTTCCATCC
  AATGACAGCTCCAAGTTCAAAACCACCGAATCACACATGGACTGGGAAAAAGTAGCATTT
  AAAGACTTTTCTGGAGACATGTGCAAGCTCAAATGGGTGGAGATTTCTAATGAGGTGAGG
  AAGTTCCGTACATTGACAGAATTGATCCTCGATGCTCAGGAACATGTTAAAAATCCTTAC
  AAAGGCAAAAAACTCAAGAAACACCCAGACTTCCCAAAGAAGCCCCTGACCCCTTATTTC
  CGCTTCTTCATGGAGAAGCGGGCCAAGTATGCGAAACTCCACCCTGAGATGAGCAACCTG
  GACCTAACCAAGATTCTGTCCAAGAAATACAAGGAGCTTCCGGAGAAGAAGAAGATGAAA
  TATATTCAGGACTTCCAGAGAGAGAAACAGGAGTTCGAGCGAAACCTGGCCCGATTCAGG
  GAGGATCACCCCGACCTAATCCAGAATGCCAAGAAATCGGACATCCCAGAGAAGCCCAAA
  ACCCCCCAGCAGCTGTGGTACACCCACGAGAAGAAGGTGTATCTCAAAGTGCGGCCAGAT
  GCCACTACGAAGGAGGTGAAGGACTCCCTGGGGAAGCAGTGGTCTCAGCTCTCGGACAAA
  AAGAGGCTGAAATGGATTCATAAGGCCCTGGAGCAGCGGAAGGAGTACGAGGAGATCATG
  AGAGACTATATCCAGAAGCACCCAGAGCTGAACATCAGTGAGGAGGGTATCACCAAGTCC
  ACCCTCACCAAGGCCGAACGCCAGCTCAAGGACAAGTTTGACGGGCGACCCACCAAGCCA
  CCTCCGAACAGCTACTCGCTGTACTGCGCAGAGCTCATGGCCAACATGAAGGACGTGCCC
  AGCACAGAGCGCATGGTGCTGTGCAGCCAGCAGTGGAAGCTGCTGTCCCAGAAGGAGAAG
  GACGCCTATCACAAGAAGTGTGATCAGAAAAAGAAAGATTACGAGGTGGAGCTGCTCCGT
  TTCCTCGAGAGCCTGCCTGAGGAGGAGCAGCAGCGGGTCTTGGGGGAAGAGAAGATGCTG
  AACATCAACAAGAAGCAGGCCACCAGCCCCGCCTCCAAGAAGCCAGCCCAGGAAGGGGGC
  AAGGGCGGCTCCGAGAAGCCCAAGCGGCCCGTGTCGGCCATGTTCATCTTCTCGGAGGAG
  AAACGGCGGCAGCTGCAGGAGGAGCGGCCTGAGCTCTCCGAGAGCGAGCTGACCCGCCTG
  CTGGCCCGAATGTGGAACGACCTGTCTGAGAAGAAGAAGGCCAAGTACAAGGCCCGAGAG
  GCGGCGCTCAAGGCTCAGTCGGAGAGGAAGCCCGGCGGGGAGCGCGAGGAACGGGGCAAG
  CTGCCCGAGTCCCCCAAAAGAGCTGAGGAGATCTGGCAACAGAGCGTTATCGGCGACTAC
  CTGGCCCGCTTCAAGAATGACCGGGTGAAGGCCTTGAAAGCCATGGAAATGACCTGGAAT
  AACATGGAAAAGAAGGAGAAACTGATGTGGATTAAGAAAGCAGCCGAAGACCAAAAGCGA
  TATGAGAGAGAGCTGAGTGAGATGCGGGCACCTCCAGCTGCTACAAATTCTTCCAAGAAG
  ATGAAATTCCAGGGAGAACCCAAGAAGCCTCCCATGAACGGTTACCAGAAGTTCTCCCAG
  GAGCTGCTGTCCAATGGGGAGCTGAACCACCTGCCGCTGAAGGAGCGCATGGTGGAGATC
  GGCAGTCGCTGGCAGCGCATCTCCCAGAGCCAGAAGGAGCACTACAAAAAGCTGGCCGAG
  GAGCAGCAAAAGCAGTACAAGGTGCACCTGGACCTCTGGGTTAAGAGCCTGTCTCCCCAG
  GACCGTGCAGCATATAAAGAGTACATCTCCAATAAACGTAAGAGCATGACCAAGCTGCGA
  GGCCCAAACCCCAAATCCAGCCGGACTACTCTGCAGTCCAAGTCGGAGTCCGAGGAGGAT
  GATGAAGAGGATGAGGATGACGAGGACGAGGATGAAGAAGAGGAAGATGATGAGAATGGG
  GACTCCTCTGAAGATGGCGGCGACTCCTCTGAGTCCAGCAGCGAGGACGAGAGCGAGGAT
  GGGGATGAGAATGAAGAGGATGACGAGGACGAAGACGACGACGAGGATGACGATGAGGAT
  GAAGATAATGAGTCCGAGGGCAGCAGCTCCAGCTCCTCCTCCTCAGGGGACTCCTCAGAC
  TCTGACTCCAACTGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014233
• Insert Size: 2920 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone may be unstable or toxic at high copy number in common E. coli strain. We recommend using a lower copy number E. coli strain, such as CopyCutter strain (http://www.epibio.com/item.asp?ID=435) for transformation and plasmid preparation. Please be aware that the DNA yield could be low. Additional aliquots of this clone can be ordered from OriGene.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014233.1, NP_055048.1
• RefSeq Size: 3097 bp
• RefSeq ORF: 2295 bp
• Locus ID: 7343
• Cytogenetics: 17q21.31
• Domains: HMG
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a member of the HMG-box DNA-binding protein family. The encoded protein plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11. [provided by RefSeq, Aug 2011]'
  Transcript Variant: This variant (1) encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.