DHPS (NM_013406) Human Untagged Clone

CAT#: SC115255

DHPS (untagged)-Human deoxyhypusine synthase (DHPS), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DHPS
• Synonyms: DHS; DS; MIG13
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_013406, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAAGGTTCCCTGGAACGGGAGGCGCCAGCGGGGGCGCTGGCCGCCGTGCTAAAGCACAGCTCGACGT
  TGCCGCCCGAAAGCACCCAGGTCCGGGGCTACGACTTCAACCGCGGTGTGAATTACCGCGCACTGCTGGA
  GGCCTTCGGCACCACCGGCTTCCAAGCAACCAACTTCGGGCGCGCTGTACAGCAAGTCAATGCCATGATC
  GAGAAGAAGCTGGAACCACTGTCACAGGATGAAGACCAGCACGCGGACCTGACCCAGAGCCGCCGCCCAC
  TTACCAGCTGCACCATTTTCCTGGGATATACATCCAACCTCATCAGTTCAGGCATCCGTGAGACCATTCG
  CTACCTTGTGCAGCACAACATGGTGGACGTATTGGTGACCACAGCTGGCGGCGTGGAGGAAGACCTCATC
  AAGTGCCTGGCGCCCACATACTTGGGCGAGTTTAGCCTCAGGGGGAAGGAGCTCCGGGAGAACGGGATCA
  ATAGGATCGGAAACCTGCTGGTGCCCAATGAGAATTACTGCAAGTTTGAGGACTGGCTGATGCCCATTCT
  GGACCAGATGGTGATGGAGCAGAACACAGAGGGTGTAAAGTGGACGCCTTCTAAGATGATCGCCCGGCTG
  GGCAAGGAGATCAACAACCCAGAGTCCGTGTATTACTGGGCCCAGAAGAACCACATCCCTGTGTTTAGTC
  CCGCACTTACAGACGGCTCGCTGGGCGACATGATCTTCTTCCATTCCTACAAGAACCCGGGCCTGGTCCT
  GGACATCGTTGAGGCCCAGGAGTTTGATGGCTCTGACTCAGGTGCCCGACCAGACGAGGCTGTCTCCTGG
  GGCAAGATCCGGGTGGATGCACAGCCCGTCAAGGTCTATGCTGACGCCTCCCTGGTCTTCCCCCTGCTTG
  TGGCTGAAACCTTTGCCCAGAAGATGGATGCCTTCATGCATGAGAAGAACGAGGACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013406 unedited
  GTGATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGTCGGGGTT
  TAACGCGTTTCTGGGCCGCCGTAAGCCCGGCCTAGGGGCAGCTTTGACTCGAGAGCCGGC
  TCTAGGCGCATGGAAGGTTCCCTGGAACGGGAGGCGCCAGCGGGGGCGCTGGCCGCCGTG
  CTAAAGCACAGCTCGACGTTGCCGCCCGAAAGCACCCAGGTCCGGGGCTACGACTTCAAC
  CGCGGTGTGAATTACCGCGCACTGCTGGAGGCCTTCGGCACCACCGGCTTCCAAGCAACC
  AACTTCGGGCGCGCTGTACAGCAAGTCAATGCCATGATCGAGAAGAAGCTGGAACCACTG
  TCACAGGATGAAGACCAGCACGCGGACCTGACCCAGAGCCGCCGCCCACTTACCAGCTGC
  ACCATTTTCCTGGGATATACATCCAACCTCATCAGTTCAGGCATCCGTGAGACCATTCGC
  TACCTTGTGCAGCACAACATGGTGGACGTATTGGTGACCACAGCTGGCGGCGTGGAGGAA
  GACCTCATCAAGTGCCTGGCGCCCACATACTTGGGCGAGTTTAGCCTCAGGGGGAAGGAG
  CTCCGGGAGAACGGGATCAATAGGATCGGAAACCTGCTGGTGCCCAATGAGAATTACTGC
  AAGTTTGAGGACTGGCTGATGCCCATTCTGGACCAGATGGTGATGGAGCAGACACAGAGG
  GTGTAAAGTGGACGCCTTCTAAGATGATCGCCCGGCTGGCCAGGAGATCACACCCAGAGT
  CCGTGTATTACTGGCCCAGAGACCAATTCCTGTGGTTTAGTCCGCCTTACGACGGCTCGC
  TGGCGACATGATCTTCTTCATTCTCAGGACCGGGGCCTGGTCTGGAATTATN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013406 unedited
  GGTACTCTATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTGACTTCAGATACCATCTTATTCTAGAGACGTAGCTGACCAAAAATAGGGGAGG
  GGCTGGGTCTGCAAATTAATAAATAAAAGAGGGGGTAAGACCTTCCTGGGACCGCAGCCG
  CTCAGTCCTCGTTCTTCTCATGCATGAAGGCATCCATCTTCTGGGCAAAGGTTTCAGCCA
  CAAGCAGGGGGAAGACCAGGGAGGCGTCAGCATAGACCTTGACGGGCTGTGCATCCACCC
  GGATCTTGCCCCAGGAGACAGCCTCGTCTGGTCGGGCACCTGAGTCAGAGCCATCAAACT
  CCTGGGCTGTGTTGATGTAAACAGCGTAGTCGGCCCCGTTCCGCATGAGGTTGGCATTGG
  CAATGTGGTGCTTGACCACGCCCCCGCCCAGAATGATCATCCCAGTGCACTTGGCAAAGA
  TGGCCTGTGTGTTGATGAGCCTCAGGTCCTCAACGATGTCCAGGACCAGGCCCGGGTTCT
  TGTAGGAATGGAAGAAGATCATGTCGCCCAGCGAGCCGTCTGTAAGTGCGGGACTAAACA
  CAGGGATGTGGTTCTTCTGGGCCCAGTAATACACGGACTCTGGGTTGTTGATCTCCTTGC
  CCAGCCGGGCGATCATCTTAGAAGGCGTCCACTTTACACCCTCTGTGTTCTGCTCCATCA
  CCATCTGGTCCAGAATGGGCATCAGCCAGTCCTCAAACTTGCAGTAATTCTCATTGGGCA
  CCAGCAGGTTTCCGATCCTATTGATCCCGTTCTCCCGGACTCCTTCCCCCTGAGCTAAAC
  TCGCCAAGTTGTGGGCGCCAGGCCTTGATGAGGTCTTCCTNCACGCGCCAGCTGTGGTCA
  CAATACGTCACCATGTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013406
• Insert Size: 1430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013406.1, NP_037538.1
• RefSeq Size: 1210 bp
• RefSeq ORF: 969 bp
• Locus ID: 1725
• Cytogenetics: 19p13.13
• Domains: DS
• Gene Summary: 'This gene encodes a protein that is required for the formation of hypusine, a unique amino acid formed by the posttranslational modification of only one protein, eukaryotic translation initiation factor 5A. The encoded protein catalyzes the first step in hypusine formation by transferring the butylamine moiety of spermidine to a specific lysine residue of the eukaryotic translation initiation factor 5A precursor, forming an intermediate deoxyhypusine residue. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2011]'
  Transcript Variant: This variant (2) lacks an exon and uses an alternate splice site in the 3' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.