ALIX (PDCD6IP) (NM_013374) Human Untagged Clone

CAT#: SC115270

PDCD6IP (untagged)-Human programmed cell death 6 interacting protein (PDCD6IP), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDCD6IP
• Synonyms: AIP1; ALIX; DRIP4; HP95
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115270 sequence for NM_013374 edited (data generated by NextGen Sequencing)
  ATGGCGACATTCATCTCGGTGCAGCTGAAAAAGACCTCAGAGGTGGACCTGGCCAAGCCG
  CTGGTGAAGTTCATCCAGCAGACTTACCCAAGCGGCGGGGAAGAGCAGGCCCAGTACTGC
  CGCGCGGCGGAGGAGCTCAGCAAGCTGCGCCGCGCCGCAGTCGGTCGTCCGCTGGACAAG
  CACGAGGGCGCGCTCGAGACGCTCCTGAGATATTATGATCAGATTTGTTCTATTGAACCC
  AAATTCCCATTTTCTGAAAATCAGATCTGCTTGACATTTACCTGGAAGGATGCTTTCGAT
  AAAGGTTCACTTTTTGGAGGCTCTGTAAAACTGGCTCTTGCAAGCTTAGGATATGAAAAG
  AGCTGTGTGTTGTTCAATTGTGCAGCCTTAGCTAGCCAAATTGCAGCAGAACAGAACCTG
  GATAATGATGAAGGATTGAAAATCGCTGCTAAACATTACCAGTTTGCTAGTGGTGCCTTT
  TTACATATTAAAGAGACGGTTTTATCTGCCTTAAGTCGAGAGCCGACCGTGGACATATCT
  CCAGATACTGTTGGGACCCTCAGTCTTATTATGCTGGCACAGGCTCAAGAAGTATTTTTT
  TTAAAAGCCACAAGAGATAAAATGAAAGATGCCATCATAGCTAAATTGGCTAATCAGGCT
  GCAGATTATTTTGGTGATGCTTTCAAACAGTGTCAATACAAAGATACTCTCCCCAAGGAG
  GTGTTCCCTGTCTTGGCTGCAAAGCACTGTATCATGCAGGCCAATGCTGAGTACCATCAG
  TCTATCCTGGCAAAACAGCAGAAGAAATTTGGAGAAGAAATTGCAAGGTTACAGCATGCA
  GCAGAACTGATTAAAACAGTGGCATCTCGCTATGATGAATATGTTAATGTGAAGGATTTT
  TCTGACAAAATCAATCGTGCCCTTGCTGCAGCAAAGAAGGATAATGACTTCATTTATCAT
  GATCGAGTTCCAGACCTTAAAGATCTAGATCCTATTGGCAAAGCCACACTTGTGAAATCT
  ACCCCGGTCAATGTACCCATCAGTCAGAAATTTACTGATCTGTTTGAGAAGATGGTTCCC
  GTGTCAGTACAGCAGTCTTTGGCTGCCTATAATCAGAGGAAAGCCGATTTGATTAACAGA
  TCAATTGCTCAGATGAGAGAAGCCACCACTTTGGCAAATGGGGTGCTAGCTTCCCTTAAT
  CTTCCAGCAGCAATTGAAGATGTGTCTGGAGACACTGTACCTCAGTCTATATTGACTAAA
  TCCAGATCTGTGATTGAACAGGGAGGCATCCAGACTGTTGATCAGTTGATTAAAGAACTG
  CCTGAATTACTGCAACGAAATAGAGAAATCCTAGATGAGTCATTAAGGTTGTTGGATGAA
  GAAGAAGCAACCGATAATGATTTAAGAGCAAAATTTAAGGAACGTTGGCAAAGGACACCA
  TCCAATGAACTGTATAAGCCTTTAAGAGCAGAGGGAACCAACTTCAGAACAGTTTTAGAT
  AAAGCTGTGCAGGCAGATGGACAAGTGAAAGAATGTTACCAGTCTCATCGTGACACCATC
  GTGCTTTTGTGTAAGCCAGAGCCTGAGCTGAATGCTGCCATCCCTTCTGCTAATCCAGCA
  AAGACCATGCAGGGCAGTGAGGTTGTAAGTGTCTTAAAATCCTTATTGTCAAATCTTGAT
  GAAGTAAAGAAGGAAAGAGAGGGTCTGGAGAATGACTTGAAATCTGTGAATTTTGACATG
  ACAAGCAAGTTTTTGACAGCCCTGGCTCAAGATGGTGTGATAAATGAAGAAGCTCTTTCT
  GTTACTGAACTAGATCGAGTCTATGGAGGTCTTACAACTAAAGTCCAAGAATCTCTAAAG
  AAACAGGAGGGACTTCTTAAAAATATTCAGGTCTCACATCAGGAATTTTCAAAAATGAAA
  CAATCTAATAATGAAGCTAACTTAAGAGAAGAAGTTTTGAAGAATTTAGCTACTGCATAT
  GACAACTTTGTTGAACTTGTAGCTAATTTGAAGGAAGGCACAAAGTTTTACAATGAGTTG
  ACTGAAATCCTGGTCAGGTTCCAGAACAAATGCAGTGATATAGTTTTTGCACGGAAGACA
  GAAAGAGATGAACTCTTAAAGGACTTGCAACAAAGCATTGCCAGAGAACCTAGTGCTCCT
  TCAATTCCTACACCTGCGTATCAGTCCTCACCAGCAGGAGGACATGCACCAACTCCTCCA
  ACTCCAGCGCCAAGAACCATGCCGCCTACTAAGCCCCAGCCCCCAGCCAGGCCTCCACCA
  CCTGTGCTTCCAGCAAATCGAGCTCCTTCTGCTACTGCTCCATCTCCAGTGGGGGCTGGG
  ACTGCTGCGCCAGCTCCATCACAAACGCCTGGCTCAGCTCCTCCTCCACAGGCGCAGGGA
  CCACCCTATCCCACCTATCCAGGATATCCTGGGTATTGCCAAATGCCCATGCCCATGGGC
  TATAATCCTTATGCGTATGGCCAGTATAATATGCCATATCCACCAGTGTATCACCAGAGT
  CCTGGACAGGCTCCGTACCCGGGACCCCAGCAGCCTTCATACCCCTTCCCTCAGCCCCCA
  CAGCAGTCTTACTATCCACAGCAGTAA
  
  Clone variation with respect to NM_013374.4
  1132 g=>a;1649 a=>g;2535 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013374 unedited
  GTTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTGTCAGCCA
  GTCAGTCCGCCAGTCCGCCAGCCCAGTACCTCTCTCTCCTCGGCCCTCGTAAGCTGTCCG
  CGGTCTGTTTGGCCCGAACGGCGGCGGAGGCGCTGATCATGGCGACATTCATCTCGGTGC
  AGNCTGAAAAAGACCTCAGAGGTGGACCTGGCCAAGCCGCTGGTGAAGTTCATCCAGCAG
  ACTTACCCAAGCGGCGGGGAAGAGCAGGCCCAGTACTGCCGCGCGGCGGAGGAGCTCAGC
  AAGCTGCGCCGCGCCGCAGTCGGTCGTCCGCTGGACAAGCACGAGGGCGCGCTCGAGACG
  CTCCTGAGATATTATGATCAGATTTGTTCTATTGAACCCAAATTCCCATTTTCTGAAAAT
  CAGATCTGCTTGACATTTACCTGGAAGGATGCTTTCGATAAAGGTTCACTTTTTGGAGGC
  TCTGTAAAACTGGCTCTTGCAAGCTTAGGATATGAAAAGAGCTGTGTGTTGTTCAATTGT
  GCAGCCTTAGCTAGCCAAATTGCAGCAGAACAGAACCTGNATAATGATGAAGGATTGAAA
  ATCGCTGCTAAACATTACCAGTTTGCTAGTGGTGCCTTTTTACATATTAAAGAGACGGTT
  NTATCTGCCTTAAGTCGAGAGCCGACCGTGGACATATCTCCAGATACTGTTGGGACCCTC
  AGTCTTATTATGCTGGCACAGGCTCAAGAAGTATTTTTTTTTAAAGCCACAAGAGATAAA
  TGAAAGATGCCATCATAGCTAAATGGNCTAATCANGCTGCAGATATTTTGGTGATGCCTT
  CAAACAGTGTCAATACAAAGATACTCTCCCCAAGGNNAGTGTCCCTGTCTTGGCTGCAAG
  CACTGTATCATGCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013374 unedited
  TGGACGCGGCCGCAATCTAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTAACGGGTCTTAAGAAATTTATTTTAAAATTATAAAATTTACAGGGCCTTGATT
  ATGCAAAATAGCATAATGGAAATTAAACCAAATCAATAAACCAAAGAGAAAGAAAACTTA
  ATTTTCTTTAGTATCCATACTTAAACCATTTTTGTAAGTATCTGATGTCCCAACCATGTT
  TTATGTAAAAAGTATAATCGTTTCAAAGGTTTCACTTGCAGGTTAAATTTCTCATTTTCA
  ATTTTTATGAACTGTAATGCAATTTCAAATCCTATTATACCTAGGGTTTATACTGCAACA
  GCAGCAAATCTCACATGGGTAATCAAATGGGGAACTGGGGCACAGCTTCTAGCTGTAAAC
  AGAAATTATACACTGCATTCAGTCCAGGAGAGTACATTACATTAACCAGAGCGTAGAGTT
  TAGTACACTTATTGCAGGGCGGGTATTTCTTTCCCTCTGATCTGAATCAGCTGAGCTGGT
  GAGCAGACATATTACTGCTGGGGATAGTAAAACTGCTGTGGGGGCTGAGGGAAGGGGTAT
  GAAGGCTGCTGGGGTCCCGGGTATGGAGCCCTGTCCAGCACTCTGGAGATACACTGGGGG
  ATATGGAATATTATACTGCCCATACGCATAAGGATTATAGCCCCATGGGCCATGGCGATT
  TGGAAATACCCAGGATATTCTGAAAAGGTGGGAAAAGGGTGGCCCCTGCCCCTGTGGAGG
  AGGAGCTGACCACGCCGTTGAGAGGAACTTGCGCCACATTCCCAGCCCCCATGTAGATGG
  AACATAACAAAAGGACCTCGATTTGCTGGAAACACAGGGGTGGAGCCTGGCTGGGGCTGG
  GCTTAAAAGCGGATGGCCTTGTGCCCGAATTGGAGGAATTGCGCATGTCCTCTGCGGAGA
  GACTGAACCCAGTGTAGGATAGAAG
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_013374
• ORF Size: 2607 bp
• Insert Size: 3330
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_013374.3, NP_037506.2
• RefSeq Size: 6448
• RefSeq ORF: 2607
• Locus ID: 10015
• Domains: BRO1
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis
• Gene Summary: This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012]
  Transcript Variant: This variant (1) uses an alternate in-frame splice site in the central coding region, compared to variant 2, resulting in an isoform (1) that is shorter than isoform 2.