ALIX (PDCD6IP) (NM_013374) Human Untagged Clone

CAT#: SC323909

PDCD6IP (untagged)-Human programmed cell death 6 interacting protein (PDCD6IP), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDCD6IP
• Synonyms: AIP1; ALIX; DRIP4; HP95
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_013374.3 CTGTCAGCCAGTCAGTCCGCCAGTCCGCCAGCCCAGTACCTCTCTCTCCTCGGCCCTCGT
  AAGCTGTCCGCGGTCTGTTTGGCCCGAACGGCGGCGGAGGCGCTGATCATGGCGACATTC
  ATCTCGGTGCAGCTGAAAAAGACCTCAGAGGTGGACCTGGCCAAGCCGCTGGTGAAGTTC
  ATCCAGCAGACTTACCCAAGCGGCGGGGAAGAGCAGGCCCAGTACTGCCGCGCGGCGGAG
  GAGCTCAGCAAGCTGCGCCGCGCCGCAGTCGGTCGTCCGCTGGACAAGCACGAGGGCGCG
  CTCGAGACGCTCCTGAGATATTATGATCAGATTTGTTCTATTGAACCCAAATTCCCATTT
  TCTGAAAATCAGATCTGCTTGACATTTACCTGGAAGGATGCTTTCGATAAAGGTTCACTT
  TTTGGAGGCTCTGTAAAACTGGCTCTTGCAAGCTTAGGATATGAAAAGAGCTGTGTGTTG
  TTCAATTGTGCAGCCTTAGCTAGCCAAATTGCAGCAGAACAGAACCTGGATAATGATGAA
  GGATTGAAAATCGCTGCTAAACATTACCAGTTTGCTAGTGGTGCCTTTTTACATATTAAA
  GAGACGGTTTTATCTGCCTTAAGTCGAGAGCCGACCGTGGACATATCTCCAGATACTGTT
  GGGACCCTCAGTCTTATTATGCTGGCACAGGCTCAAGAAGTATTTTTTTTAAAAGCCACA
  AGAGATAAAATGAAAGATGCCATCATAGCTAAATTGGCTAATCAGGCTGCAGATTATTTT
  GGTGATGCTTTCAAACAGTGTCAATACAAAGATACTCTCCCCAAGGAGGTGTTCCCTGTC
  TTGGCTGCAAAGCACTGTATCATGCAGGCCAATGCTGAGTACCATCAGTCTATCCTGGCA
  AAACAGCAGAAGAAATTTGGAGAAGAAATTGCAAGGTTACAGCATGCAGCAGAACTGATT
  AAAACAGTGGCATCTCGCTATGATGAATATGTTAATGTGAAGGATTTTTCTGACAAAATC
  AATCGTGCCCTTGCTGCAGCAAAGAAGGATAATGACTTCATTTATCATGATCGAGTTCCA
  GACCTTAAAGATCTAGATCCTATTGGCAAAGCCACACTTGTGAAATCTACCCCGGTCAAT
  GTACCCATCAGTCAGAAATTTACTGATCTGTTTGAGAAGATGGTTCCCGTGTCAGTACAG
  CAGTCTTTGGCTGCCTATAATCAGAGGAAAGCCGATTTGGTTAACAGATCAATTGCTCAG
  ATGAGAGAAGCCACCACTTTGGCAAATGGGGTGCTAGCTTCCCTTAATCTTCCAGCAGCA
  ATTGAAGATGTGTCTGGAGACACTGTACCTCAGTCTATATTGACTAAATCCAGATCTGTG
  ATTGAACAGGGAGGCATCCAGACTGTTGATCAGTTGATTAAAGAACTGCCTGAATTACTG
  CAACGAAATAGAGAAATCCTAGATGAGTCATTAAGGTTGTTGGATGAAGAAGAAGCAACC
  GATAATGATTTAAGAGCAAAATTTAAGGAACGTTGGCAAAGGACACCATCCAATGAACTG
  TATAAGCCTTTAAGAGCAGAGGGAACCAACTTCAGAACAGTTTTAGATAAAGCTGTGCAG
  GCAGATGGACAAGTGAAAGAATGTTACCAGTCTCATCGTGACACCATCGTGCTTTTGTGT
  AAGCCAGAGCCTGAGCTGAATGCTGCCATCCCTTCTGCTAATCCAGCAAAGACCATGCAG
  GGCAGTGAGGTTGTAAATGTCTTAAAATCCTTATTGTCAAATCTTGATGAAGTAAAGAAG
  GAAAGAGAGGGTCTGGAGAATGACTTGAAATCTGTGAATTTTGACATGACAAGCAAGTTT
  TTGACAGCCCTGGCTCAAGATGGTGTGATAAATGAAGAAGCTCTTTCTGTTACTGAACTA
  GATCGAGTCTATGGAGGTCTTACAACTAAAGTCCAAGAATCTCTAAAGAAACAGGAGGGA
  CTTCTTAAAAATATTCAGGTCTCACATCAGGAATTTTCAAAAATGAAACAATCTAATAAT
  GAAGCTAACTTAAGAGAAGAAGTTTTGAAGAATTTAGCTACTGCATATGACAACTTTGTT
  GAACTTGTAGCTAATTTGAAGGAAGGCACAAAGTTTTACAATGAGTTGACTGAAATCCTG
  GTCAGGTTCCAGAACAAATGCAGTGATATAGTTTTTGCACGGAAGACAGAAAGAGATGAA
  CTCTTAAAGGACTTGCAACAAAGCATTGCCAGAGAACCTAGTGCTCCTTCAATTCCTACA
  CCTGCGTATCAGTCCTCACCAGCAGGAGGACATGCACCAACTCCTCCAACTCCAGCGCCA
  AGAACCATGCCGCCTACTAAGCCCCAGCCCCCAGCCAGGCCTCCACCACCTGTGCTTCCA
  GCAAATCGAGCTCCTTCTGCTACTGCTCCATCTCCAGTGGGGGCTGGGACTGCTGCGCCA
  GCTCCATCACAAACGCCTGGCTCAGCTCCTCCTCCACAGGCGCAGGGACCACCCTATCCC
  ACCTATCCAGGATATCCTGGGTATTGCCAAATGCCCATGCCCATGGGCTATAATCCTTAT
  GCGTATGGCCAGTATAATATGCCATATCCACCAGTGTATCACCAGAGTCCTGGACAGGCT
  CCATACCCGGGACCCCAGCAGCCTTCATACCCCTTCCCTCAGCCCCCACAGCAGTCTTAC
  TATCCACAGCAGTAATATGTCTGCTCAGCAGCTCAGCTGATTCAGATCAGAGGGAAAGAA
  ATACCAACCCTGCAATAAGTGTACTAAACTCTACGCTCTGGTTAATGTAATGTACTCTCC
  TGGACTGAATGCAGTGTATAATTTCTGTCTACAGCTAGAAGCTGTGCCCCAGTTCCACAT
  TTGATTACACATGTGAGATTTGCTGCTGTTGCAGTATAAACACTAGGTATAATAGGATTT
  GAAATTGCATTACAGTTCATAAAAATTGAAAATGAGAAATTAAACCTGCAAGTGAAACAT
  TTGAAACGATTATACTTTCTACATAAGACATGGTTGGGACATCAGATACTTACAAAGATG
  GTTTAAGTATGGATACTAGAGAAAATTAAGTTTTCTTTCTCTTTGGTTTATTGATTTGGT
  TTAATTTCCATTATGCTATTTTGCATAATCAAGGCACTGTAAATCTTATAATTTTAAAAT
  AAATTACTTAAGAACAGTTGTCATTGTTATGTTTTGTTATTGATTCTCATTACTGTCTAA
  TTTTTTTTCTGGTATTAGTCTCATTTTGTATGTATATAAGTTAAACAGATACTGTTTTTA
  AGTGCATGAATAGTACAAGTTATTATCAAGGATGTTTTACAGGGAAATCAAAAGAATATT
  ATCATACTTTATCTTTCGTATGCTGATTAGTAAACGATTTTTGACATTTATTTTAGAAAG
  TCCTATAATGTGGAAGAAACAAACAGTTGCTACCAAAGATGATGAAAATAAACATACAAA
  TAAATGTGTATATTTAATGTTTTATTGTTAGCTTCTCCAGAAAATTGATGCAAATTCTGG
  TAATAATTCTTGCATTTTTTCCCCATAACCTGGTTAAAATAAATACGCCATTGGCAATAA
  AAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013374.3 unedited
  GACGCTTGTATACGACTCCTATAGGGCGGCCGGCGATCGCGGCGCGCCGGTACCGGTCCG
  GAATTCCCGGGATATCGTCGACCCACGCGTCCGCTGTCAGCCAGTCAGTCCGCCAGTCCG
  CCAGCCCAGTACCTCTCTCTCCTCGGCCCTCGTAAGCTGTCCGCGGTCTGTTTGGCCCGA
  ACGGCGGCGGAGGCGCTGATCATGGCGACATTCATCTCGGTGCAGCTGAAAAAGACCTCA
  GAGGTGGACCTGGCCAAGCCGCTGGTGAAGTTCATCCAGCAGACTTACCCAAGCGGCGGG
  GAAGAGCAGGCCCAGTACTGCCGCGCGGCGGAGGAGCTCAGCAAGCTGCGCCGCGCCGCA
  GTCGGTCGTCCGCTGGACAAGCACGAGGGCGCGCTCGAGACGCTCCTGAGATATTATGAT
  CAGATTTGTTCTATTGAACCCAAATTCCCATTTTCTGAAAATCAGATCTGCTTGACATTT
  ACCTGGAAGGATGCTTTCGATAAAGGTTCACTTTTTGGAGGCTCTGTAAAACTGGCTCTT
  GCAAGCTTAGGATATGAAAAGAGCTGTGTGTTGTTCAATTGTGCAGCCTTAGCTAGCCAA
  ATTGCAGCAGACAGGACCCTGGATAATGATGAAGGATTGAAAATCGCTGCTAAACATTAC
  CAGTTTGCTAGTGGTGCCTTTTTACATATTAAAGAGACGGTTTTTATCTGCCTTAAGTCG
  AGAGCCCGACCGTGGAACATATCTTCAGAATACTGTTGGGACCCTCATCTAATTATGCTG
  GCACAGGCTCAGATGTATTTTTTTTTAAAGCCACAGAGATAATATGAAAGATGCCTCATA
  ACTTACTTGGCTAACCAGCCTGCGCAT
• Restriction Sites: ECoRI-NOT
• ACCN: NM_013374
• ORF Size: 2607 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_013374.3, NP_037506.2
• RefSeq Size: 6448
• RefSeq ORF: 2607
• Locus ID: 10015
• Domains: BRO1
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis
• Gene Summary: This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012]
  Transcript Variant: This variant (1) uses an alternate in-frame splice site in the central coding region, compared to variant 2, resulting in an isoform (1) that is shorter than isoform 2.