VPS4A (NM_013245) Human Untagged Clone

CAT#: SC115346

VPS4A (untagged)-Human vacuolar protein sorting 4 homolog A (S. cerevisiae) (VPS4A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VPS4A
• Synonyms: SKD1; SKD1A; SKD2; VPS4; VPS4-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115346 sequence for NM_013245 edited (data generated by NextGen Sequencing)
  ATGACAACGTCAACCCTCCAGAAAGCCATTGATCTGGTGACGAAAGCCACAGAGGAGGAC
  AAAGCCAAGAACTACGAGGAGGCGCTGCGGCTGTACCAGCATGCGGTGGAGTACTTCCTC
  CACGCTATCAAGTATGAGGCCCACAGCGACAAGGCCAAGGAGAGCATTCGAGCCAAGTGC
  GTGCAGTACCTAGACCGGGCCGAGAAGCTGAAGGATTATTTACGAAGCAAAGAGAAACAC
  GGCAAGAAGCCAGTCAAAGAGAACCAGAGTGAGGGCAAGGGCAGTGACAGTGACAGTGAA
  GGGGATAATCCGGAGAAAAAGAAACTGCAAGAACAGCTGATGGGTGCCGTCGTGATGGAG
  AAGCCCAACATACGGTGGAACGACGTGGCCGGGCTGGAGGGGGCCAAGGAGGCCCTCAAA
  GAAGCTGTCATTTTGCCAATCAAATTCCCACACTTGTTCACAGGCAAGCGCACCCCCTGG
  CGGGGGATTCTGCTGTTCGGACCCCCTGGCACAGGGAAATCCTACCTGGCCAAAGCCGTG
  GCAACAGAGGCCAACAACTCCACCTTCTTCTCTGTGTCCTCCTCAGATCTGATGTCCAAG
  TGGCTGGGGGAGAGTGAAAAGCTGGTCAAGAACCTGTTTGAGCTGGCCAGGCAGCACAAG
  CCCTCCATCATCTTCATCGATGAGGTGGATTCCCTCTGCGGGTCCCGAAATGAAAATGAG
  AGTGAGGCCGCCCGGAGGATCAAAACGGAGTTCTTGGTCCAGATGCAGGGGGTGGGGAAT
  AACAATGATGGGACTCTGGTTCTTGGAGCCACAAACATCCCATGGGTGTTGGATTCGGCC
  ATCAGGAGGAGGTTTGAAAAGCGAATTTATATCCCCTTGCCGGAGGAAGCTGCCCGCGCC
  CAGATGTTCCGGTTGCATCTCGGGAGCACTCCCCACAACCTCACGGATGCAAACATCCAC
  GAGCTGGCCCGGAAGACGGAAGGCTACTCGGGCGCGGACATCAGCATCATCGTGCGGGAC
  TCTCTCATGCAGCCCGTGAGGAAGGTGCAGTCGGCCACACACTTCAAAAAGGTCTGTGGC
  CCCTCTCGCACCAACCCCAGCATGATGATTGATGACCTCCTGACTCCATGCTCACCAGGG
  GACCCAGGAGCCATGGAGATGACTTGGATGGATGTCCCTGGGGACAAACTCTTAGAGCCT
  GTGGTTTGCATGTCGGACATGCTGCGGTCTCTGGCCACCACCCGGCCCACGGTGAATGCA
  GACGACCTCCTGAAAGTGAAGAAATTCTCAGAGGACTTTGGGCAAGAGAGTTAA
  
  Clone variation with respect to NM_013245.2
  861 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013245 unedited
  GTTCGGATTTTGTAATACGACTTACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGGAC
  CGAGGCCGCAAGCAGCGCCGCGGGGTGTGGGGCGGACCCAGGAGATGAAATGACAACGTC
  AACCCTCCAGAAAGCCATTGATCTGGTGACGAAAGCCACAGAGGAGGACAAAGCCAAGAA
  CTACGAGGAGGCGCTGCGGCTGTACCAGCATGCGGTGGAGTACTTCCTCCACGCTATCAA
  GTATGAGGCCCACAGCGACAAGGCCAAGGAGAGCATTCGAGCCAAGTGCGTGCAGTACCT
  AGACCGGGCCGAGAAGCTGAAGGATTATTTACGAAGCAAAGAGAAACACGGCAAGAAGCC
  AGTCAAAGAGAACCAGAGTGAGGGCAAGGGCAGTGACAGTGACAGTGAAGGGGATAATCC
  GGAGAAAAAGAAACTGCAAGAACAGCTGATGGGTGCCGTCGTGATGGAGAAGCCCAACAT
  ACGGTGGAACGACGTGGCCGGGCTGGAGGGGGCCAAGGAGGCCCTCAAAGAAGCTGTCAT
  TTTGCCAATCAAATTCCCACACTTGTTCACAGGCAAGCGCACCCCCTGGCGGGGGATTCT
  GCTGTTCGGACCCCCTGGCACAGGGAAATCCTACCTGGCCAAAGCCGTGGCAACAGAGGC
  CAACAACTCCACCTTCTTCTCTGTGTCCTCCTCAGATCTGATGTCCACATGGCTGGGGGA
  GAGTGAAAAGCTGGTCAAGAACCTGTTTGAGCTGGGCCAGCAGCACAAGCCCTCCATCAT
  CTTCATCGATGAGGTGGATTCCCTCTGCGGGTCCCGAAATGAAATGAGAGTGAGGCCGNC
  CGGAGGATCAAAACGGAGTTCTTGGTCCAATGCAGGGGGGTGGGGGAAATACAATGATGG
  NACTCTGGTTCCTTGGAGCCACAACTAACTCGGGGGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013245 unedited
  ATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTAGCTCAAAAAGGGAAAAGGATTTATTAAACCATTTCCAGGACAGGGACCCAGG
  GGCAGTGGCTGGGTTCAGTGTTCTCACCCTCAAAGGAAAAAAAGGCAGTGGTGAACACCC
  GCCTGACTGCCTTACCCTGATCTAAACGACACCATTTCCTCCCCCAAGAAAAACACAGCC
  CTGGATGTGAACCTAGGAGGGTGGGAATGGCTTATGTCACTCGGTCTTTGAGGCAAAACT
  TGGGCTGGGGTTGCAGGGGCATCAACCAATCTGCTCTCAAGGAGCAAAACTTGGGCGTAA
  AAGGAACGGGCAGTGGGGCTAGCCGGCTGTCTGGAGGTCTGGGTGACCTGGCAACACGGG
  TGTTTTTCCCCCGCCACCCCGCCCCACTTCTTGACACTTCCAGGTGATTTTATCTTTATA
  AATAAGATGAAAATCCAAGCCGCATTAATTTAGGGGAACAAAAGATGGAAAAAAAAAGGG
  GGGGAGGCAGAAAAAGCTGATGAGCCTCCAGGAGAGGAGGAAGTAGGAAGAGCAGTGTCC
  CCTGTGTGCCCCAGGGCAGTGGAGTGTCTCTGTGGCTGAAGGCCCTTCCTTCTGGCTCCC
  TGGCAGACGGCCAGACAGCAGAGGGAACTCTGTATTGACTGGGACAAGCCCTGNAGCCCT
  GTCTGGCTGTTGACATGGGGAAGCCTGGATTTGCCCAATTACCTTCCACTTCCCATTGCC
  CAGTGAGCAGCTTTTACTCTCTGGCCAAAGTCTTGAGAATTCTTCACTTTCAGAGGCGCT
  GCATCACGTGGGCGGNTGGGGNCAAAACGCANTTGTCCACTGCAACCCAGTTTAAATTTG
  CCCAAGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013245
• ORF Size: 1314 bp
• Insert Size: 2090
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_013245.2, NP_037377.1
• RefSeq Size: 2211
• RefSeq ORF: 1314
• Locus ID: 27183
• Domains: AAA, AAA, MIT
• Protein Pathways: Endocytosis
• Gene Summary: The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18. [provided by RefSeq, Jul 2008]