FMO3 (NM_006894) Human Untagged Clone

CAT#: SC115806

FMO3 (untagged)-Human flavin containing monooxygenase 3 (FMO3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FMO3
• Synonyms: dJ127D3.1; FMOII; TMAU
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115806 sequence for NM_006894 edited (data generated by NextGen Sequencing)
  ATGGGGAAGAAAGTGGCCATCATTGGAGCTGGTGTGAGTGGCTTGGCCTCCATCAGGAGC
  TGTCTGGAAGAGGGGCTGGAGCCCACCTGCTTTGAGAAGAGCAATGACATTGGGGGCCTG
  TGGAAATTTTCAGACCATGCAGAGGAGGGCAGGGCTAGCATTTACAAATCAGTCTTTTCC
  AACTCTTCCAAAGAGATGATGTGTTTCCCAGACTTCCCATTTCCCGATGACTTCCCCAAC
  TTTATGCACAACAGCAAGATCCAGGAATATATCATTGCATTTGCCAAAGAAAAGAACCTC
  CTGAAGTACATACAATTTAAGACATTTGTATCCAGTGTAAATAAACATCCTGATTTTGCA
  ACTACTGGCCAGTGGGATGTTACCACTGAAAGGGATGGTAAAAAAGAATCGGCTGTCTTT
  GATGCTGTAATGGTTTGTTCCGGACATCATGTGTATCCCAACCTACCAAAAGAGTCCTTT
  CCAGGACTAAACCACTTTAAAGGCAAATGCTTCCACAGCAGGGACTATAAAGAACCAGGT
  GTATTCAATGGAAAGCGTGTCCTGGTGGTTGGCCTGGGGAATTCGGGCTGTGATATTGCC
  ACAGAACTCAGCCGCACAGCAGAACAGGTCATGATCAGTTCCAGAAGTGGCTCCTGGGTG
  ATGAGCCGGGTCTGGGACAATGGTTATCCTTGGGACATGCTGCTCGTCACTCGATTTGGA
  ACCTTCCTCAAGAACAATTTACCGACAGCCATCTCTGACTGGTTGTACATGAAGCAGATG
  AATGCAAGATTCAAGCATGAAAACTATGGCTTGATGCCTTTAAATGGAGTCCTGAGGAAA
  GAGCCTGTATTTAACGATGAGCTCCCAGCAAGCATTCTGTGTGGCATTGTGTCCGTAAAG
  CCTAACGTGAAGGAATTCACAGAGACCTCGGCCATTTTTGAGGATGGGACCATATTTGAG
  GGCATTGACTGTGTAATCTTTGCAACAGGGTATAGTTTTGCCTACCCCTTCCTTGATGAG
  TCTATCATCAAAAGCAGAAACAATGAGATCATTTTATTTAAAGGAGTATTTCCTCCTCTA
  CTTGAGAAGTCAACCATAGCAGTGATTGGCTTTGTCCAGTCCCTTGGGGCTGCCATTCCC
  ACAGTTGACCTCCAGTCCCGCTGGGCAGCACAAGTAATAAAGGGAACTTGTACTTTGCCT
  TCTATGGAAGACATGATGAATGATATTAATGAGAAAATGGAGAAAAAGCGCAAATGGTTT
  GGCAAAAGCGAGACCATACAGACAGATTACATTGTTTATATGGATGAACTCTCCTCCTTC
  ATTGGGGCAAAGCCCAACATCCCATGGCTGTTTCTCACAGATCCCAAATTGGCCATGGAA
  GTTTATTTTGGCCCTTGTAGTCCCTACCAGTTTAGGCTGGTGGGCCCAGGGCAGTGGCCA
  GGAGCCAGAAATGCCATACTGACCCAGTGGGACCGGTCGTTGAAACCCATGCAGACACGA
  GTGGTCGGGAGACTTCAGAAGCCTTGCTTTTTTTTCCATTGGCTGAAGCTCTTTGCAATT
  CCTATTCTGTTAATCGCTGTTTTCCTTGTGTTGACCTAA
  
  Clone variation with respect to NM_006894.5
  769 g=>a;1530 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006894 unedited
  GCACCAGAAGGTTGGACAGGACGTAGACACACAGAAGAAAAGAAGACAAAGAACGGGTAG
  GAAAATTAAAAAGGTTACCATGGNGGAAGAAAGTGGCCATCATTGGAGCTGGTGTGAGTG
  GCTTGGCCTCCATCAGGAGCTGTCTGGAAGAGGGGCTGGAGCCCACCTGCTTTGAGAAGA
  GCAATGACATTGGGGGCCTGTGGAAATTTTCAGACCATGCAGAGGAGGGCAGGGCTAGCA
  TTTACAAATCAGTCTTTTCCAACTCTTCCAAAGAGATGATGTGTTTCCCAGACTTCCCAT
  TTCCCGATGACTTCCCCAACTTTATGCACAACAGCAAGATCCAGGAATATATCATTGCAT
  TTGCCAAAGAAAAGAACCTCCTGAAGTACATACAATTTAAGACATTTGTATCCAGTGTAA
  ATAAACATCCTGATTTTGCAACTACTGGCCAGTGGGATGTTACCACTGAAAGGGATGGTA
  AAAAAGAATCGGCTGTCTTTGATGCTGTAATGGTTTGTTCCGGACATCATGTGTATCCCA
  ACCTACCAAAAGAGTCCTTTCCAGGACTAAACCACTTTAAAGGCAAATGCTTCCACAGCA
  GGGACTATAAAGAACCAGGTGTATTCAATGGAAAGCGTGTCCTGGNTGGTTGGCCTGGGG
  AAATTCGGCTGTGATATTGCCACAGAACTCAGCCGCACAGCAGAAACAGGTCATGATCAG
  TTNCCAGAAGTGGCTCCTGGGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006894 unedited
  NNNNTTTACTTGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTATAATTATATCTGCTTAATTTGCTCTTTATTTAAATTTAAAATACATCTTT
  CATACATTGTTTAAAAGGAAAAAGGAATCATGTCAAAATGTTTCAGGAAATGATTTAAGT
  TAAATATAAAGAACATAGTTCTGATTTTGAAGTGTTAGTGGAACTCAAACAGAAATGATT
  AGTGCTCTCTTAAGATCATGACATGATTCTTAAAGTGGCTATTTCAAAGCCTAAAAATAA
  CACTGTATTACTGACTAATGTCTACAGAGTACTGCAAAAGATGCTGAATAGAAAAGCAGG
  TGGTGTGAAAATTTTAATTTTTAAATAGCAAAGCCCCTGTCTGGGTATTGTCAGTAACTT
  TCAAAAATCCTAGAGAAAATGATGATTAGGTCAACACAAGGAAAACAGCGATTAACAGAA
  TAGGAATTGCAAAGAGCTTCAGCCAATGGAAAAAAAAGCAAGGCTTCTGAAGTCTCCCGA
  CCACTCGTGTCTGCATGGGTTTCAACGACCGGTCCCACTGGGTCAGTATGGCATTTCTGG
  CTCCTGGCCACTGCCCTGGGCCCACCAGCCTAAACTGGTAGGGACTACAAGGGCCAAAAT
  AAACTTCCATGGNCCAATTTGGGATCTGTGAGAAACAGCCATGGGATGTTGGGCTTTGCC
  CCAATGAAGGAGGGAGAGTTCATCCATATAAACAATGTAATCTGTCTGTATGGTCTCGCT
  TTTGCCAAACATTTGCNGCTTTTCTNCTTNTTCTCATTAATATCATTNCATCATGTCTCC
  ATAGAAGGGCAAGTACAGTTCCTTTTATTACTGTGCTGCCCAGCGGACTGGAGGGTCACT
  GTGGAATGCAGCCCCAGGGACTGGACAAGCCATACTGCTATGGTGACTTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006894
• Insert Size: 2140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006894.4, NP_008825.4
• RefSeq Size: 2053 bp
• RefSeq ORF: 1599 bp
• Locus ID: 2328
• Cytogenetics: 1q24.3
• Domains: FMO-like
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Drug metabolism - cytochrome P450
• Gene Summary: 'Flavin-containing monooxygenases (FMO) are an important class of drug-metabolizing enzymes that catalyze the NADPH-dependent oxygenation of various nitrogen-,sulfur-, and phosphorous-containing xenobiotics such as therapeutic drugs, dietary compounds, pesticides, and other foreign compounds. The human FMO gene family is composed of 5 genes and multiple pseudogenes. FMO members have distinct developmental- and tissue-specific expression patterns. The expression of this FMO3 gene, the major FMO expressed in adult liver, can vary up to 20-fold between individuals. This inter-individual variation in FMO3 expression levels is likely to have significant effects on the rate at which xenobiotics are metabolised and, therefore, is of considerable interest to the pharmaceutical industry. This transmembrane protein localizes to the endoplasmic reticulum of many tissues. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Mutations in this gene cause the disorder trimethylaminuria (TMAu) which is characterized by the accumulation and excretion of unmetabolized trimethylamine and a distinctive body odor. In healthy individuals, trimethylamine is primarily converted to the non odorous trimethylamine N-oxide.[provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1 and 2 both encode the same isoform (a).