5T4 (TPBG) (NM_006670) Human Untagged Clone

CAT#: SC115948

TPBG (untagged)-Human trophoblast glycoprotein (TPBG), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TPBG
• Synonyms: 5T4; 5T4AG; M6P1; WAIF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006670 edited
  GAATTCGGCACGAGGGGGGAATCGGCCCCTGAGGGAAGCGCCCGGTGGCGAGGGGGTTAG
  CCAAGTTCCGGCTGCGGCGCCACTCCCTCGGTTCCACGAGAGGAAAGTTTTTTTTTTCCA
  GACGCTTCCGCCGGCTCGCGCCCTCCGGGCCCAGCCTCCCGAGCCTTCGGAGCGGGCGCC
  GTCCCAGCCCAGCTCCGGGGAAACGCGAGCCGCGATGCCTGGGGGGTGCTCCCGGGGCCC
  CGCCGCCGGGGACGGGCGTCTGCGGCTGGCGCGACTAGCGCTGGTACTCCTGGGCTGGGT
  CTCCTCGTCTTCTCCCACCTCCTCGGCATCCTCCTTCTCCTCCTCGGCGCCGTTCCTGGC
  TTCCGCCGTGTCCGCCCAGCCCCCGCTGCCGGACCAGTGCCCCGCGCTGTGCGAGTGCTC
  CGAGGCAGCGCGCACAGTCAAGTGCGTTAACCGCAATCTGACCGAGGTGCCCACGGACCT
  GCCCGCCTACGTGCGCAACCTCTTCCTTACCGGCAACCAGCTGGCCGTGCTCCCTGCCGG
  CGCCTTCGCCCGCCGGCCGCCGCTGGCGGAGCTGGCCGCGCTCAACCTCAGCGGCAGCCG
  CCTGGACGAGGTGCGCGCGGGCGCCTTCGAGCATCTGCCCAGCCTGCGCCAGCTCGACCT
  CAGCCACAACCCACTGGCCGACCTCAGTCCCTTCGCTTTCTCGGGCAGCAATGCCAGCGT
  CTCGGCCCCCAGTCCCCTTGTGGAACTGATCCTGAACCACATCGTGCCCCCTGAAGATGA
  GCGGCAGAACCGGAGCTTCGAGGGCATGGTGGTGGCGGCCCTGCTGGCGGGCCGTGCACT
  GCAGGGGCTCCGCCGCTTGGAGCTGGCCAGCAACCACTTCCTTTACCTGCCGCGGGATGT
  GCTGGCCCAACTGCCCAGCCTCAGGCACCTGGACTTAAGTAATAATTCGCTGGTGAGCCT
  GACCTACGTGTCCTTCCGCAACCTGACACATCTAGAAAGCCTCCACCTGGAGGACAATGC
  CCTCAAGGTCCTTCACAATGGCACCCTGGCTGAGTTGCAAGGTCTACCCCACATTAGGGT
  TTTCCTGGACAACAATCCCTGGGTCTGCGACTGCCACATGGCAGACATGGTGACCTGGCT
  CAAGGAAACAGAGGTAGTGCAGGGCAAAGACCGGCTCACCTGTGCATATCCGGAAAAAAT
  GAGGAATCGGGTCCTCTTGGAACTCAACAGTGCTGACCTGGACTGTGACCCGATTCTTCC
  CCCATCCCTGCAAACCTCTTATGTCTTCCTGGGTATTGTTTTAGCCCTGATAGGCGCTAT
  TTTCCTCCTGGTTTTGTATTTGAACCGCAAGGGGATAAAAAAGTGGATGCATAACATCAG
  AGATGCCTGCAGGGATCACATGGAAGGGTATCATTACAGATATGAAATCAATGCGGACCC
  CAGATTAACAAACCTCAGTTCTAACTCGGATGTCTGAGAAATATTAGAGGACAGACCAAG
  GACAACTCTGCATGAGATGTAGACTTAAGCTTTATCCCTACTAGGCTTGCTCCACTTTCA
  TCCTCCACTATAGATACAACGGACTTTGACTAAAAGCAGTGAAGGGGATTTGCTTCCTTG
  TTATGTAAAGTTTCTCGGTGTGTTCTGTTAATGTAAGACGATGAACAGTTGTGTATAGTG
  TTTTACCCTCTTCTTTTTCTTGGAACTCCTCAACACGTATGGAGGGATTTTTCAGGTTTC
  AGCATGAACATGGGCTTCTTGCTGTCTGTCTCTCTCTCAGTACAGTTCAAGGTGTAGCAA
  GTGTACCCACACAGATAGCATTCAACAAAAGCTGCCTCAACTTTTTCGAGAAAAATACTT
  TATTCATAAATATCAGTTTTATTCTCATGTACCTAAGTTGTGGAGAAAATAATTGCATCC
  TATAAACTGCCTGCAGACGTTAGCAGGCTCTTCAAAATAACTCCATGGTGCACAGGAGCA
  CCTGCATCCAAGAGCATGCTTACATTTTACTGTTCTGCATATTACAAAAAATAACTTGCA
  ACTTCATAACTTCTTTGACAAAGTAAATTACTTTTTTGATTGCAGTTTATATGAAAATGT
  ACTGATTTTTTTTTAATAAACTGCATCGAGATCCAACCGACTGAATTGTTAAAAAAAAAA
  AAAATAAAGATTCTTAAAAGAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006670 unedited
  CCGCATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACCGGGGGGAATCGGCCC
  CTGAGGGAAGCGCCCGGTGGCGAGGGGGTTAGCCAAGTTCCGGCTGCGGCGCCACTCCCT
  CGGTTCCACGAGAGGAAAGTTTTTTTTTTCCAGACGCTTCCGCCGGCTCGCGCCCTCCGG
  GCCCAGCCTCCCGAGCCTTCGGAGCGGGCGCCGTCCCAGCCCAGCTCCGGGGAAACGCGA
  GCCGCGATGCCTGGGGGGTGCTCCCGGGGCCCCGCCGCCGGGGACGGGCGTCTGCGGCTG
  GCGCGACTAGCGCTGGTACTCCTGGGCTGGGTCTCCTCGTCTTCTCCCACCTCCTCGGCA
  TCCTCCTTCTCCTCCTCGGCGCCGTTCCTGGCTTCCGCCGTGTCCGCCCAGCCCCCGCTG
  CCGGACCAGTGCCCCGCGCTGTGCGAGTGCTCCGAGGCAGCGCGCACAGTCAAGTGCGTT
  AACCGCAATCTGACCGAGGTGCCCACGGACCTGCCCGCCTACGTGCGCAACCTCTTCCTT
  ACCGGCAACCAGCTGGCCGTGCTCCCTGCCGGCGCCTTCGCCCGCCGGCCGCCGCTGGCG
  GAGCTGGCCGCGCTCAACCTCAGCGGCAGCCGCCTGGACGAGGTGCGCGCGGGCGCCTTC
  GAGCATCTGCCCAGCCTGCGCCAGCTCGACCTCAGCCACAACCCACTGGCCGACCTCAGT
  CCCTTCGCTTTCTCGGGCAGCAATGCCAGCGTCTCGGCCCCCAGTCCCCTTGTGGAACTG
  ATCCTNGACCACATCGTGCCCCCCTGNAAGAGANCGGCAGAACCGGAGCTTCNAGGGCAT
  GGTGGTGGGCGCCCTGCTGGCGGGCCGTGCACTGCAGGGGCTCCGNCGCTTGGAGCTGGN
  CAGCACCACTTNCTTTACTGNCGGGGAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006670 unedited
  ACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTCTTTTAAAAATC
  TTTATTTTTTTTTTTTTTAACAATTCAGTCGGTTGGATCTCGATGCAGTTTATTAAAAAA
  AAATCAGTACATTTTCATATAAACTGCAATCAAAAAAGTAATTTACTTTGTCAAAGAAGT
  TATGAAGTTGCAAGTTATTTTTTGTAATATGCAGAACAGTAAAATGTAAGCATGCTCTTG
  GATGCAGGTGCTCCTGTGCACCATGGAGTTATTTTGAAAAGCCTGCTAACGTCTGCAGGC
  AGTTTATAGGATGCAATTATTTTCTCCACAACTTAGGTACATGAGAATAAAACTGATATT
  TATGAATAAAGTATTTTTCTCGAAAAAGTTGAGGCAGCTTTTGTTGAATGCTATCTGTGT
  GGGTACACTTGCTACACCTTGAACTGTACTGAGAGAGAGACAGACAGCAAGAAGCCCATG
  TTCATGCTGAAACCTGAAAAATCCCTCCATACGTGTTGAGGAGTTCCAAGAAAAAGAAGA
  GGGTAAAACACTATACACAACTGTTCATCGTCTTACATTAACAGAACACACCGAGAAACT
  TTACATAACAAGGAAGCAAAACCCCTTCACTGTTTTTAGACAAAGCCCGTTGTATTCTAT
  AGTGGAGGATGAAAGTGGAGCAAGCCTACTATGGATAAAAGCTAAGCCTACATCTCATGC
  CAAGGTGACCTTGGTCCTGCCCTCCAATAATTCCTCACACATCCGGAGTAGCACCTGAAG
  TTTGTTAATTCTGGGGGCCCCATTGATTTCATATCTGGAATGGAACCCTCTCCTGGGAAC
  CCCTGCAGGCATTCTCTGAAGTTAGGCCTCACCTTTTTTATCCCCTTGGGGCGTAAAAAC
  CAACCCGGGGGGAAATACCGCCTATCGGGCCTACACAACTCCCGGAAGACTCTAGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006670
• Insert Size: 2150 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006670.3, NP_006661.1
• RefSeq Size: 2379 bp
• RefSeq ORF: 1263 bp
• Locus ID: 7162
• Cytogenetics: 6q14.1
• Domains: LRRNT, LRRCT, LRR, LRR_TYP
• Protein Families: Transmembrane
• Gene Summary: 'This gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. The encoded protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is associated with poor clinical outcome in numerous cancers. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2009]'
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.