USP20 (NM_006676) Human Untagged Clone

CAT#: SC115950

USP20 (untagged)-Human ubiquitin specific peptidase 20 (USP20), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: USP20
• Synonyms: hVDU2; LSFR3A; VDU2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006676 edited
  TTCGGCACGAGGGTTGCGAGTGCAGGCTCCTTGCCAGAGGCCTCCACTCACTCCAGACCC
  CTATAGCCCGTCGCTGTCAGCTGTCAACAAAGGATGCGAATGCTGGCCGCTTCCTGTGGG
  CTTCGTGTCACCCAGAGGTGAGCCCAGGCCAGGATGGGGGACTCCAGGGACCTTTGCCCT
  CACCTTGACTCCATAGGAGAGGTGACCAAAGAGGACTTGCTGCTCAAATCTAAGGGAACC
  TGTCAGTCGTGTGGGGTCACCGGACCAAACCTATGGGCCTGTCTGCAGGTTGCCTGCCCC
  TATGTTGGCTGCGGAGAATCCTTCGCTGACCACAGCACCATTCATGCACAGGCAAAAAAG
  CACAACTTGACCGTGAACCTGACCACGTTCCGACTGTGGTGTTACGCCTGTGAGAAGGAG
  GTATTCCTGGAGCAGCGGCTGGCAGCCCCTCTGCTGGGCTCCTCTTCCAAGTTCTCTGAA
  CAGGACTCCCCGCCACCCTCCCACCCTCTGAAAGCTGTTCCTATTGCTGTGGCTGATGAA
  GGAGAGTCTGAGTCAGAGGATGATGACCTGAAACCTCGAGGCCTCACGGGCATGAAGAAC
  CTCGGGAACTCCTGCTACATGAACGCCGCCCTGCAGGCCCTGTCCAATTGCCCGCCGCTG
  ACTCAGTTCTTCTTGGAGTGTGGCGGCCTGGTGCGCACAGATAAGAAGCCAGCCCTGTGC
  AAGAGCTACCAGAAGCTGGTCTCTGAGGTCTGGCATAAGAAACGGCCAAGCTACGTGGTC
  CCCACCAGTCTGTCTCATGGGATCAAGTTGGTCAACCCAATGTTCCGAGGCTATGCCCAG
  CAGGACACCCAAGAGTTCCTTCGCTGCCTGATGGACCAGCTGCACGAGGAGCTCAAGGAG
  CCGGTGGTGGCCACGGTGGCGCTGACGGAGGCTCGGGACTCAGATTCGAGTGACACGGAT
  GAGAAACGGGAGGGTGACCGGAGCCCATCAGAAGATGAGTTCTTGTCCTGTGACTCGAGC
  AGTGACCGGGGTGAGGGTGACGGGCAGGGGCGTGGCGGGGGCAGCTCGCAGGCCGAGACG
  GAGCTGCTGATCCCAGATGAGGCGGGCCGAGCCATCTCTGAGAAGGAGCGGATGAAGGAC
  CGCAAGTTCTCCTGGGGCCAGCAGCGTACAAACTCGGAGCAAGTGGACGAGGACGCTGAT
  GTGGACACTGCCATGGCTGCCCTTGACCAGCCCGCGGAGGCCCAGCCCCCGTCACCACGG
  TCCTCCAGCCCCTGCCGGACGCCAGAGCCGGACAATGATGCTCACCTACGCAGCTCCTCT
  CGCCCCTGCAGCCCCGTCCACCACCACGAGGGCCATGCCAAGCTGTCTAGCAGCCCCCCT
  CGTGCAAGCCCCGTGAGGATGGCACCGTCGTACGTGCTCAAGAAAGCCCAGGTATTGAGT
  GCTGGCAGCCGGAGGCGGAAGGAGCAGCGCTACCGCAGCGTCATCTCAGACATCTTTGAC
  GGCTCCATTCTCAGCCTCGTGCAGTGTCTCACCTGTGACCGGGTATCCACCACAGTGGAA
  ACGTTCCAGGACTTATCACTGCCCATTCCTGGAAAGGAGGACCTGGCCAAGCTCCATTCA
  GCCATCTACCAGAATGTGCCGGCCAAGCCAGGCGCCTGTGGGGACAGCTATGCCGCCCAG
  GGCTGGCTGGCCTTCATTGTGGAGTACATCCGACGGTTTGTGGTATCCTGTACCCCCAGC
  TGGTTTTGGGGGCCTGTCGTCACCCTGGAAGACTGCCTTGCTGCCTTCTTTGCCGCTGAT
  GAGTTAAAGGGTGACAACATGTACAGCTGTGAGCGGTGTAAGAAGCTGCGGAACGGAGTG
  AAGTACTGCAAAGTCCTGCGGTTGCCCGAGATCCTGTGCATTCACCTAAAGCGCTTTCGG
  CACGAGGTGATGTACTCATTCAAGATCAACAGCCACGTCTCCTTCCCCCTCGAGGGGCTC
  GACCTGCGCCCCTTCCTTGCCAAGGAGTGCACATCCCAGATCACCACCTACGACCTCCTC
  TCGGTCATCTGCCACCACGGCACGGCAGGCAGTGGGCACTACATCGCCTACTGCCAGAAC
  GTGATCAATGGGCAGTGGTACGAGTTTGATGACCAGTACGTCACAGAAGTCCACGAGACG
  GTGGTGCAGAACGCCGAGGGCTACGTACTCTTCTACAGGAAGAGCAGCGAGGAGGCCATG
  CGGGAGCGACAGCAGGTGGTGTCCCTGGCCGCCATGCGGGAGCCCAGCCTGCTGCGGTTC
  TACGTGTCCCGCGAGTGGCTCAACAAGTTCAACACCTTCGCGGAGCCAGGCCCCATCACC
  AACCAGACCTTCCTCTGCTCCCACGGAGGCATCCCGCCCCACAAATACCACTACATCGAC
  GACCTGGTGGTCATCCTGCCCCAGAACGTCTGGGAGCACCTGTACAACAGATTCGGGGGT
  GGCCCCGCCGTGAACCACCTGTACGTGTGCTCCATCTGCCAGGTGGAGATCGAGGCACTG
  GCCAAGCGCAGGAGGATCGAGATCGACACCTTCATCAAGTTGAACAAGGCCTTCCAGGCC
  GAGGAGTCGCCGGGCGTCATCTACTGCATCAGCATGCAGTGGTTCCGGGAGTGGGAGGCG
  TTCGTCAAGGGGAAGGACAACGAGCCCCCCGGGCCCATTGACAACAGCAGGATTGCACAG
  GTCAAAGGAAGCGGCCATGTCCAGCTGAAGCAGGGAGCTGACTACGGGCAGATTTCGGAG
  GAGACCTGGACCTACCTGAACAGCCTGTATGGAGGTGGCCCCGAGATTGCCATCCGCCAG
  AGTGTGGCGCAGCCGCTGGGCCCAGAGAACCTGCACGGGGAGCAGAAGATCGAAGCCGAG
  ACGCGGGCCGTGTGATCTGCTGGGCTAGTCTCCCCATGTGCCCCACCCCGCGGAAGGCGT
  GTTTGTGCCCAGAAGAGAGGCCGGGCTGCTGCAGAACCCCGCCGTGTAAAGAGGCAGAAA
  AGTTGGTTTGGTTTGCAGTAACGCTGCAACTAGAAAATATATGCACTTCAGGCTTGTTGA
  AACGACCAAGACTCTGTGACGTTAATTTGGGTCTTTGTCCTGGCAGTGCCTCTGCCAGTC
  ACTGTCATCGTTGTGTCCCCCACAACTGTCCTCTTGCTAGCTCGGCCCAGCTTTGTCCCT
  GGAGCCCGATGCTACCCCTGTCAGACAGAGGCTGCGGCCTG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006676 unedited
  GGGTCGTCTCCTACTTTCGCACGAGGGTTGCGAGTGCATGCTTCTTGCCAGAGGTCTCCA
  CTCACTCCAGACCCCTATAGCCCGTCGCTGTCAGCTGTCAACAAAGGATGCGAATGCTGG
  CCGCTTTCTGTGGGCTTCGTGTCACCCAGAGGTGAGCCCAGGCCAGGATGGGGGACTCCA
  GGGACCTTTGCCCTCACCTTGACTCCATAGGAGAGGTGACCAAAGAGGACTTGCTGCTCA
  AATCTAAGGGAACCTGTCAGTCGTGTGGGGTCACCGGACCAAACCTATGGGCCTGTCTGC
  AGGTTGCCTGCCCCTATGTTGGCTGCGGAGAATCCTTCGCTGACCACAGCACCATTCATG
  CACAGGCAAAAAAGCACAACTTGACCGTGAACCTGACCACGTTCCGACTGTGGTGTTACG
  CCTGTGAGAAGGAGGTATTCCTGGAGCAGCGGCTGGCAGCCCCTCTGCTGGGCTCCTCTT
  CCAAGTTCTCTGAACAGGACTCCCCGCCACCCTTCCACCCTCTGAAAGCTGTTCCTATTG
  CTGTGGCTGATGAAGGAGAGTCTGAGTCAGAAGATGATGACCTGAAACCTCGAGGCCTCA
  CGGGCATGAAGAACCTCGGGAACTCCTGCTCATGAACGCCGCCCTGCAAGCCCTGTTCAT
  TGCCCGCCGCTGACTCAGNTTTTCTTTGAAGTGTGGCGGGCTGGTGCGCACAGATTAGGA
  ACCAGCCCTGTGCAAGAACTACCAAAAGCTGGTCTTTGAAGTCTGCCTTAAGAAACGGCC
  AAGCTACGTGGGTCCCCACAGTCTGTTCCAAGGGATCAAAGTTGGCAACCCCATGGTCCC
  AGGCTTTGCCCAAGAGGACACCCAAGAGTTCCTTCCCTGCCTGAAGGACCCCTGCACCAG
  AACCTAAGAGCCGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006676 unedited
  NTTTTTTCTATGGACCCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTAAC
  ATTTATTCTGTGTTTATTCTGACATTTTGAACACAAACTAGACAGCACAGACATGGGGCC
  CGATCCCAGCCTGCACAGAAGCCTCTCTGCGTCGCGACAGTGCAGCGTGCGGCCTCTGGC
  TGCAGCGCCGGCTTTCAGAAGGCTGGGGCCAATGCCAGGGGAACCCCAGGCTCAGCAGGG
  AGGGAGCAGGATCTGGGACAGAAGATCCGAAAAGTGAGGCCTCAGGGCCTCTGCGGGACC
  CACCTGCTGGAATTTCCACCACCACCTGCTTCTACAAAGACTGATAGAAACAAAGACGCA
  GTAGTGTGGGGGGTCTGGTCCCAGAGCCCCTTCCCCACCCACGCCCCGTCTGCTTTGATC
  CTGATGATGGTCGCACCTCGCCCTGGCGGTTTCTCCAGGGGCTTTGGTGCCTTGCTGTGC
  TCAGTGGCTCCCTCCACTCAGCATCCAGAGGCTGCTTCCCAGGATCCACCTGAGCGCTGC
  ACCCTGCACAGCCCCCACATCCCTCCAGGCGGGCTCTGCACTGCTGCTGCTGGGAGGAAG
  GGAAAGTCTGTCTTCTTTAGCATCAGCTCGGGAAGCTGCCTTTGCCTCAAATGGCCAAGC
  TGTGAGGTATCCCTGTTTCATCATCCGGTTCCCTGGTGGGGGCTCAGCAAATTTCCCATG
  AAGATGAACTGCTGAACTTCGAGGCAAATTACAGCCAATCCAGGGGGCAGGCTCAGCAGG
  ACGCGGGTGTGGGGGACCAGCTGCCTCCAGAAACATCCCCCACTGGCAGAAATCCAACCT
  CCTCCTGGTGTTCCAATCAGCAATGAGTCCAGAGTCTTCAGTCTGCAGGAGAAGTGCTGA
  GAGCAGCCCGGNACTTCAGAGCCTTCCTGTTGACGCCCAGAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006676
• ORF Size: 2742 bp
• Insert Size: 4880
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_006676.4, NP_006667.2
• RefSeq Size: 4511
• RefSeq ORF: 2742
• Locus ID: 10868
• Domains: UCH, zf-UBP, DUSP
• Protein Families: Druggable Genome, Protease
• Gene Summary: This gene encodes a ubiquitin specific processing protease that was first identified as a substrate of the VHL (von Hippel-Lindau disease) protein E3 ubiquitin ligase complex. In addition to being ubiquitinated by the VHL-E3 ligase complex, this enzyme deubiquitinates hypoxia-inducible factor (HIF)-1 alpha and thereby causes increased expression of HIF-1alpha targeted genes which play a role in angiogenesis, glucose metabolism, cell proliferation and metastasis. The enzyme encoded by this gene also regulates G-protein coupled receptor signaling by mediating the deubiquitination of beta-2 adrenergic receptor (ADRB2). This enzyme is a ubiquitously expressed thiolester hydrolase. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jan 2013]
  Transcript Variant: This variant (1) represents the longest transcript. All three transcript variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments and orthologous data.