USP20 (NM_006676) Human Untagged Clone

CAT#: SC324089

USP20 (untagged)-Human ubiquitin specific peptidase 20 (USP20), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: USP20
• Synonyms: hVDU2; LSFR3A; VDU2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006676.4 CAGGCGGCGGCGGCGCAGTTGCGAGTGCAGGCTCCTTGCCAGAGGCCTCCACTCACTCCA
  GACCCCTATAGCCCGTCGCTGTCAGCTGTCAACAAAGGATGCGAATGCTGGCCGCTTCCT
  GTGGGCTTCGTGTCACCCAGAGGTGAGCCCAGGCCAGGATGGGGGACTCCAGGGACCTTT
  GCCCTCACCTTGACTCCATAGGAGAGGTGACCAAAGAGGACTTGCTGCTCAAATCTAAGG
  GAACCTGTCAGTCGTGTGGGGTCACCGGACCAAACCTATGGGCCTGTCTGCAGGTTGCCT
  GCCCCTATGTTGGCTGCGGAGAATCCTTCGCTGACCACAGCACCATTCATGCACAGGCAA
  AAAAGCACAACTTGACCGTGAACCTGACCACGTTCCGACTGTGGTGTTACGCCTGTGAGA
  AGGAGGTATTCCTGGAGCAGCGGCTGGCAGCCCCTCTGCTGGGCTCCTCTTCCAAGTTCT
  CTGAACAGGACTCCCCGCCACCCTCCCACCCTCTGAAAGCTGTTCCTATTGCTGTGGCTG
  ATGAAGGAGAGTCTGAGTCAGAGGATGATGACCTGAAACCTCGAGGCCTCACGGGCATGA
  AGAACCTCGGGAACTCCTGCTACATGAACGCCGCCCTGCAGGCCCTGTCCAATTGCCCGC
  CGCTGACTCAGTTCTTCTTGGAGTGTGGCGGCCTGGTGCGCACAGATAAGAAGCCAGCCC
  TGTGCAAGAGCTACCAGAAGCTGGTCTCTGAGGTCTGGCATAAGAAACGGCCAAGCTACG
  TGGTCCCCACCAGTCTGTCTCATGGGATCAAGTTGGTCAACCCAATGTTCCGAGGCTATG
  CCCAGCAGGACACCCAAGAGTTCCTTCGCTGCCTGATGGACCAGCTGCACGAGGAGCTCA
  AGGAGCCGGTGGTGGCCACGGTGGCGCTGACGGAGGCTCGGGACTCAGATTCGAGTGACA
  CGGATGAGAAACGGGAGGGTGACCGGAGCCCATCAGAAGATGAGTTCTTGTCCTGTGACT
  CGAGCAGTGACCGGGGTGAGGGTGACGGGCAGGGGCGTGGCGGGGGCAGCTCGCAGGCCG
  AGACGGAGCTGCTGATCCCAGATGAGGCGGGCCGAGCCATCTCTGAGAAGGAGCGGATGA
  AGGACCGCAAGTTCTCCTGGGGCCAGCAGCGTACAAACTCGGAGCAAGTGGACGAGGACG
  CTGATGTGGACACTGCCATGGCTGCCCTTGACCAGCCCGCGGAGGCCCAGCCCCCGTCAC
  CACGGTCCTCCAGCCCCTGCCGGACGCCAGAGCCGGACAATGATGCTCACCTACGCAGCT
  CCTCTCGCCCCTGCAGCCCCGTCCACCACCACGAGGGCCATGCCAAGCTGTCTAGCAGCC
  CCCCTCGTGCAAGCCCCGTGAGGATGGCACCGTCGTACGTGCTCAAGAAAGCCCAGGTAT
  TGAGTGCTGGCAGCCGGAGGCGGAAGGAGCAGCGCTACCGCAGCGTCATCTCAGACATCT
  TTGACGGCTCCATTCTCAGCCTCGTGCAGTGTCTCACCTGTGACCGGGTATCCACCACAG
  TGGAAACGTTCCAGGACTTATCACTGCCCATTCCTGGAAAGGAGGACCTGGCCAAGCTCC
  ATTCAGCCATCTACCAGAATGTGCCGGCCAAGCCAGGCGCCTGTGGGGACAGCTATGCCG
  CCCAGGGCTGGCTGGCCTTCATTGTGGAGTACATCCGACGGTTTGTGGTATCCTGTACCC
  CCAGCTGGTTTTGGGGGCCTGTCGTCACCCTGGAAGACTGCCTTGCTGCCTTCTTTGCCG
  CTGATGAGTTAAAGGGTGACAACATGTACAGCTGTGAGCGGTGTAAGAAGCTGCGGAACG
  GAGTGAAGTACTGCAAAGTCCTGCGGTTGCCCGAGATCCTGTGCATTCACCTAAAGCGCT
  TTCGGCACGAGGTGATGTACTCATTCAAGATCAACAGCCACGTCTCCTTCCCCCTCGAGG
  GGCTCGACCTGCGCCCCTTCCTTGCCAAGGAGTGCACATCCCAGATCACCACCTACGACC
  TCCTCTCGGTCATCTGCCACCACGGCACGGCAGGCAGTGGGCACTACATCGCCTACTGCC
  AGAACGTGATCAATGGGCAGTGGTACGAGTTTGATGACCAGTACGTCACAGAAGTCCACG
  AGACGGTGGTGCAGAACGCCGAGGGCTACGTACTCTTCTACAGGAAGAGCAGCGAGGAGG
  CCATGCGGGAGCGACAGCAGGTGGTGTCCCTGGCCGCCATGCGGGAGCCCAGCCTGCTGC
  GGTTCTACGTGTCCCGCGAGTGGCTCAACAAGTTCAACACCTTCGCGGAGCCAGGCCCCA
  TCACCAACCAGACCTTCCTCTGCTCCCACGGAGGCATCCCGCCCCACAAATACCACTACA
  TCGACGACCTGGTGGTCATCCTGCCCCAGAACGTCTGGGAGCACCTGTACAACAGATTCG
  GGGGTGGCCCCGCCGTGAACCACCTGTACGTGTGCTCCATCTGCCAGGTGGAGATCGAGG
  CACTGGCCAAGCGCAGGAGGATCGAGATCGACACCTTCATCAAGTTGAACAAGGCCTTCC
  AGGCCGAGGAGTCGCCGGGCGTCATCTACTGCATCAGCATGCAGTGGTTCCGGGAGTGGG
  AGGCGTTCGTCAAGGGGAAGGACAACGAGCCCCCCGGGCCCATTGACAACAGCAGGATTG
  CACAGGTCAAAGGAAGCGGCCATGTCCAGCTGAAGCAGGGAGCTGACTACGGGCAGATTT
  CGGAGGAGACCTGGACCTACCTGAACAGCCTGTATGGAGGTGGCCCCGAGATTGCCATCC
  GCCAGAGTGTGGCGCAGCCGCTGGGCCCAGAGAACCTGCACGGGGAGCAGAAGATCGAAG
  CCGAGACGCGGGCCGTGTGATCTGCTGGGCTAGTCTGTAAGTCGCCCCGGCTGGTCCCTC
  CATGGCACTCTGGGTCCTCTCCTCACTCTCCAGAGACCCTCACATGTCCTTTTGAACATC
  CAAAGAGCAGGTCCCTGAAAGCACCTTCCTGGAGGATGTGGGAGGGCCCTGGACATGGCC
  CGGCCCCACTGCTGAGTGCCCGTGTCCCCACAGCCCCATGTGCCCCACCCCGCGGAAGGC
  GTGTTTGTGCCCAGAAGAGAGGCCGGGCTGCTGCAGAACCCCGCCGTGTAAAGAGGCAGA
  AAAGTTGGTTTGGTTTGCAGTAACGCTGCAACTAGAAAATATATGCACTTCAGGCTTGTT
  GAAACGACCAAGACTCTGTGACGTTAATTTGGGTCTTTGTCCTGGCAGTGCCTCTGCCAG
  TCACTGTCATCGTTGTGTCCCCCACAACTGTCCTCTTGCTAGCTCGGCCCAGCTTTGTCC
  CTGGAGCCCGATGCTACCCCTGTCAGACAGAGGCTGCGGCCTGGGCCAGAGTCAGGGAGT
  AGCTGCTGCTTCACGGCGTCTCCACTGTGCGATTGGCCCGGAGCCCCGAAGACTCGGAGG
  GAGCTGCTCAGGGCCGGTGAGCGCAGCCAGAAGCCCTGGCCAGTGAGGAGCTCACAGGTC
  CTCCCTGGTGGTCCCGCCGCACCTCTGCATCTCCTGGGCGTCACCAGGAAGGCTCTGAAG
  TCCCGGGCTGCTCTCAGCACTTCTCCTGCAGACTGAAGACTCTGGACTCATTGCTGATTG
  GAACACCAGGAGGAGGTTGGATTTCTGCCAGTGGGGGATGTTTCTGGAGGCAGCTGGTCC
  CCCACACCGCGTCCTGCTGAGCCTGCCCCCTGGATTGGCTGTAATTTGCCTCGAAGTTCA
  GCAGTTCATCTTCATGGGAAATTTGCTGAGCCCCCACCAGGGAACCGGATGATGAAACAG
  GGATACCTCACAGCTTGGCCATTTGAGGCAAAGGCAGCTTCCCGAGCTGATGCTAAAGAA
  GACAGACTTTCCCTTCCTCCCAGCAGCAGCAGTGCAGAGCCCGCCTGGAGGGATGTGGGG
  GCTGTGCAGGGTGCAGCGCTCAGGTGGATCCTGGGAAGCAGCCTCTGGATGCTGAGTGGA
  GGGAGCCACTGAGCACAGCAAGGCACCAAAGCCCCTGGAGAAACCGCCAGGGCGAGGTGC
  GACCATCATCAGGATCAAAGCAGACGGGGCGTGGGTGGGGAAGGGGCTCTGGGACCAGAC
  CCCCCACACTACTGCGTCTTTGTTTCTATCAGTCTTTGTAGAAGCAGGTGGTGGTGGAAA
  TTCCAGCAGGTGGGTCCCGCAGAGGCCCTGAGGCCTCACTTTTCGGATCTTCTGTCCCAG
  ATCCTGCTCCCTCCCTGCTGAGCCTGGGGTTCCCCTGGCATTGGCCCCAGCCTTCTGAAA
  GCCGGCGCTGCAGCCAGAGGCCGCACGCTGCACTGTCGCGACGCAGAGAGGCTTCTGTGC
  AGGCTGGGATCGGGCCCCATGTCTGTGCTGTCTAGTTTGTGTTCAAAATGTCAGAATAAA
  CACAGAATAAATGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006676.4 unedited
  GGCGTTTGTATACGACTCCTATAGGGCGGCCGGCGATCGCGGCGCGCCGGTACCGGTCCG
  GAATTCCCGGGATCAGGCGGCGGCGGCGCAGTTGCGAGTGCAGGCTCCTTGCCAGAGGCC
  TCCACTCACTCCAGACCCCTATAGCCCGTCGCTGTCAGCTGTCAACAAAGGATGCGAATG
  CTGGCCGCTTCCTGTGGGCTTCGTGTCACCCAGAGGTGAGCCCAGGCCAGGATGGGGGAC
  TCCAGGGACCTTTGCCCTCACCTTGACTCCATAGGAGAGGTGACCAAAGAGGACTTGCTG
  CTCAAATCTAAGGGAACCTGTCAGTCGTGTGGGGTCACCGGACCAAACCTATGGGCCTGT
  CTGCAGGTTGCCTGCCCCTATGTTGGCTGCGGAGAATCCTTCGCTGACCACAGCACCATT
  CATGCACAGGCAAAAAAGCACAACTTGACCGTGAACCTGACCACGTTCCGACTGTGGTGT
  TACGCCTGTGAGAAGGAGGTATTCCTGGAGCAGCGGCTGGCAGCCCCTCTGCTGGGCTCC
  TCTTCCAAGTTCTCTGAACAGGACTCCCCGCCACCCTCCCACCCTCTGAAAGCTGTTCCT
  ATTGCTGTGGCTGATGAAGGAGAGTCTGAGTCAGAGGATGATGACCTGAAACCTCGAGGC
  CTCACGGGCATGAAGAACCTCGGGAACTCCTGCTACATGAACGCCGCCCTGCAGGCCCTG
  TCCAATTGCCCGCCGCTGACTCAGTTCTTCTTGGAGTGTGGCGGCCTGTGCGCACAGATA
  GAGCCAGCCCTGTGCAGAGCTACCAGAGCTGGTCCTCTGAGGTCTGGCATAGAACGGCCA
  AGCTACGTGGTCCCCACAGTTCTTGTCTCATGGAATCAAGTGTCAACCAATGTTCCGAGC
  TATGCCAGCAGGACCCCAAAGTCCTCGCTGCCTGATGGACCACCTGCACTAGGACTCAGG
  GACCCGTGATC
• Restriction Sites: ECoRI-NOT
• ACCN: NM_006676
• ORF Size: 2742 bp
• Insert Size: 4500
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_006676.4, NP_006667.2
• RefSeq Size: 4511
• RefSeq ORF: 2742
• Locus ID: 10868
• Domains: UCH, zf-UBP, DUSP
• Protein Families: Druggable Genome, Protease
• Gene Summary: This gene encodes a ubiquitin specific processing protease that was first identified as a substrate of the VHL (von Hippel-Lindau disease) protein E3 ubiquitin ligase complex. In addition to being ubiquitinated by the VHL-E3 ligase complex, this enzyme deubiquitinates hypoxia-inducible factor (HIF)-1 alpha and thereby causes increased expression of HIF-1alpha targeted genes which play a role in angiogenesis, glucose metabolism, cell proliferation and metastasis. The enzyme encoded by this gene also regulates G-protein coupled receptor signaling by mediating the deubiquitination of beta-2 adrenergic receptor (ADRB2). This enzyme is a ubiquitously expressed thiolester hydrolase. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jan 2013]
  Transcript Variant: This variant (1) represents the longest transcript. All three transcript variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments and orthologous data.