NXF1 (NM_006362) Human Untagged Clone

CAT#: SC116148

NXF1 (untagged)-Human nuclear RNA export factor 1 (NXF1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NXF1
• Synonyms: MEX67; TAP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116148 sequence for NM_006362 edited (data generated by NextGen Sequencing)
  ATGGCGGACGAGGGGAAGTCGTACAGCGAACACGATGATGAACGCGTTAATTTCCCTCAA
  AGAAAGAAGAAAGGCCGGGGTCCCTTCCGGTGGAAATATGGTGAAGGAAACCGTAGGTCT
  GGAAGAGGCGGTTCTGGTATTCGGTCTTCCCGCCTTGAGGAAGATGATGGAGATGTGGCA
  ATGAGTGATGCCCAGGATGGTCCCCGAGTACGATACAACCCCTATACCACCCGACCTAAC
  CGTCGGGGTGATACTTGGCATGATCGAGATCGCATTCATGTTACTGTGCGGAGAGACAGA
  GCTCCTCCAGAGAGAGGAGGGGCTGGCACCAGCCAGGATGGGACCTCAAAGAACTGGTTC
  AAGATTACAATTCCTTATGGCAGAAAGTATGACAAGGCATGGCTCCTGAGCATGATTCAG
  AGCAAGTGCAGTGTGCCCTTCACCCCTATTGAGTTTCACTATGAGAATACACGGGCCCAG
  TTCTTCGTTGAAGACGCCAGTACTGCCTCTGCATTGAAGGCTGTCAACTATAAGATTTTG
  GATCGGGAGAACCGAAGGATATCTATCATCATCAACTCTTCTGCTCCACCCCACACTATA
  CTGAATGAACTGAAGCCAGAACAAGTAGAACAGCTAAAGCTGATCATGAGCAAACGATAC
  GATGGCTCCCAACAAGCCCTTGACCTCAAAGGCCTCCGTTCAGACCCAGATTTGGTGGCC
  CAGAACATTGACGTTGTCCTGAATCGCAGAAGCTGTATGGCAGCTACCCTGAGGATCATT
  GAAGAGAACATCCCTGAGCTATTGTCCTTGAACTTGAGCAACAACAGGCTGTACAGGCTG
  GATGACATGTCTAGCATTGTTCAGAAGGCACCCAACCTGAAGATCCTAAACCTTTCTGGA
  AATGAATTGAAGTCTGAGCGGGAATTGGACAAGATAAAGGGGCTGAAGCTAGAAGAGCTC
  TGGCTCGATGGAAACTCCCTGTGTGACACCTTCCGAGACCAGTCCACCTACATCAGCGCC
  ATTCGCGAACGATTTCCCAAGTTACTACGCCTGGATGGCCATGAGCTACCCCCACCAATT
  GCCTTTGATGTTGAAGCCCCCACGACGTTACCGCCCTGCAAGGGAAGCTATTTTGGAACA
  GAAAACTTGAAGAGTCTGGTCTTGCACTTCCTGCAACAGTACTATGCAATTTACGACTCT
  GGAGACCGACAAGGGCTCCTGGATGCCTACCATGATGGGGCCTGCTGTTCCCTGAGCATT
  CCTTTCATTCCTCAGAACCCTGCCCGAAGCAGCTTAGCCGAGTATTTCAAGGATAGCAGA
  AATGTGAAGAAGCTTAAAGACCCTACCTTGCGGTTCCGGCTGCTGAAGCACACGCGTCTC
  AACGTTGTTGCCTTCCTCAATGAGTTGCCCAAAACCCAGCACGACGTCAATTCCTTCGTG
  GTAGACATAAGCGCCCAGACAAGCACATTGCTGTGTTTTTCTGTCAATGGAGTCTTCAAG
  GAAGTGGACGGAAAGTCCCGGGATTCTTTGCGAGCCTTCACCCGGACATTCATTGCTGTT
  CCTGCTAGCAATTCAGGGCTATGTATTGTAAATGATGAGCTATTTGTGCGGAATGCCAGT
  TCTGAAGAGATCCAAAGAGCCTTCGCTATGCCTGCACCCACGCCTTCCTCCAGCCCGGTG
  CCCACCCTCTCTCCAGAGCAGCAGGAAATGTTGCAAGCATTCTCTACCCAGTCTGGCATG
  AACCTCGAGTGGTCCCAGAAGTGCCTTCAGGACAACAACTGGGACTACACCAGATCTGCC
  CAGGCCTTCACTCATCTCAAGGCCAAGGGCGAGATCCCAGAAGTGGCATTCATGAAGTGA
  
  Clone variation with respect to NM_006362.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006362 unedited
  TTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGCGGCGCGCGAC
  GTGCTTTGCTGTATAAATGCGGTGGCGCCCGGCGTAGGGACACTTCGGTCCTGAGCGCTT
  GGGAGTTAGGTTGTTTGCCGGCGTAGCGGCCAGCGCCTGAGCCCGCCCTTGATCTTCGCT
  GTGGCATGGCGGACGAGGGGAAGTCGTACAGCGAACACGATGATGAACGCGTTAATTTCC
  CTCAAAGAAAGAAGAAAGGCCGGGGTCCCTTCCGGTGGAAATATGGTGAAGGAAACCGTA
  GGTCTGGAAGAGGCGGTTCTGGTATTCGGTCTTCCCGCCTTGAGGAAGATGATGGAGATG
  TGGCAATGAGTGATGCCCAGGATGGTCCCCGAGTACGATACAACCCCTATACCACCCGAC
  CTAACCGTCGGGGTGATACTTGGCATGATCGAGATCGCATTCATGTTACTGTGCGGAGAG
  ACAGAGCTCCTCCAGAGAGAGGAGGGGCTGGCACCAGCCAGGATGGGACCTCAAAGAACT
  GGTTCAAGATTACAATTCCTTATGGCAGAAAGTATGACAAGGCATGGCTCCTGAGCATGA
  TTCAGAGCAAGTGCAGTGTGCCCTTCACCCCTATTGAGTTTCACTATGAGAATACACGGG
  CCCAGTTCTTCGTTGAAGACGCCAGTACTGCCTCTGCATTGAAGGCTGTCAACTATAAGA
  TTTTGGATCGGGAGAACCGANAGATATCTATCATCATCAACTCTTCTGCTCCACCCCCAC
  TATACTGNATGAAACTGAGCCAGNAACAGTAGAACAGCTAAAGCTGATCATGAGCAAACG
  ATACGATGGCTCCCAACAGCCCTTGACCTCAAGGNC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006362 unedited
  GAACCGCGCCTTATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTACAACATCACTCTTTAT
  ATTAAAAAGTGCAGAACACGAAATACAACATCACTCTTTATATTAAAAAGTAAGGAGGTC
  CTGGGGTTAAGTACACAAAGCACCTAAGTCCTTCGGGTAGTTTAGTGTCAGTTCTACAAA
  GTAAGCTTTGGCTTCCTGGCACCAGTGAGGCTCAATCTTCTGAAGTCTTCCAGAAGGCAG
  GCGAGGAGAGGGATCAGGCAGCCCTCCCTCCCTCGGTCACAGTCACGGGGCGGCCTCGGG
  CCAGACAGGAGGAGATGACAGACGACAACCAGACGGTAATATCCAAGGACTATTTACAGG
  GGGGACTGCTTCTGAGGCATGACTACGATCACTTCATGAATGCCACTTCTGGGATCTCGC
  CCTTGGCCTTGAGATGAGTGAAGGCCTGGGCAGATCTGGTGTAGTCCCAGTTGTTGTCCT
  GAAGGCACTTTTGGGACCACTCGAGGTTCATGCCAGACTGGGTAGAGAATGCTTGCAACA
  TTTCCTGCTGCTCTGGAGAGAGGGTGGGCACCGGGCTGGAGGAAGGCGTGGGTGCACGCC
  TANCGAAGGCTCTTTGGATCTCTTAAGAACTGGCATTCCGCACAAATGGCTCATCATTTA
  CAATACATAGCCCCTGAATTGCTAGCAAGGACAACATTGAATGTCCGGGGGAAAGCTCCA
  AAATAACCCGGGAACTTTCCGCCCACTTTCCTTTAAAACTCCTTGGCCAGAAAAACACAN
  CAATGTGCTTGTCTTGGGCCTTTTGTCTCCCACAAAGAATTGCCCCCCGCTGGGCTCGGG
  CCCCCTCGTGGAAGCCCCCCTCGAACCCTGTGCTCACCACCGCACCGCAGGTAGGCTTAA
  ACTTTCTCATTTTTGTTTCTTAAAATAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006362
• ORF Size: 1860 bp
• Insert Size: 2640
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_006362.3, NP_006353.2
• RefSeq Size: 2307
• RefSeq ORF: 1860
• Locus ID: 10482
• Domains: LRR, NTF2, TAP_C
• Gene Summary: This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (1).