RAN (NM_006325) Human Untagged Clone

CAT#: SC116166

RAN (untagged)-Human RAN, member RAS oncogene family (RAN)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAN
• Synonyms: ARA24; Gsp1; TC4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006325 edited
  ATGGCTGCGCAGGGAGAGCCCCAGGTCCAGTTCAAACTTGTATTGGTTGGTGATGGTGGT
  ACTGGAAAAACGACCTTCGTGAAACGTCATTTGACTGGTGAATTTGAGAAGAAGTATGTA
  GCCACCTTGGGTGTTGAGGTTCATCCCCTAGTGTTCCACACCAACAGAGGACCTATTAAG
  TTCAATGTATGGGACACAGCCGGCCAGGAGAAATTCGGTGGACTGAGAGATGGCTATTAT
  ATCCAAGCCCAGTGTGCCATCATAATGTTTGATGTAACATCGAGAGTTACTTACAAGAAT
  GTGCCTAACTGGCATAGAGATCTGGTACGAGTGTGTGAAAACATCCCCATTGTGTTGTGT
  GGCAACAAAGTGGATATTAAGGACAGGAAAGTGAAGGCGAAATCCATTGTCTTCCACCGA
  AAGAAGAATCTTCAGTACTACGACATTTCTGCCAAAAGTAACTACAACTTTGAAAAGCCC
  TTCCTCTGGCTTGCTAGGAAGCTCATTGGAGACCCTAACTTGGAATTTGTTGCCATGCCT
  GCTCTCGCCCCACCAGAAGTTGTCATGGACCCAGCTTTGGCAGCACAGTATGAGCACGAC
  TTAGAGGTTGCTCAGACAACTGCTCTCCCGGATGAGGATGATGACCTGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006325 unedited
  AAGTTAGATTTGTATACGACTTATATAGGCGGCCGCGCAATTCGCACGAGGGCTTCCGCC
  ATCTTTCCAGCCTCAGTCGGACGGGCGCGGAGACGCTTCTGGAAGGAACGCCGCGATGGC
  TGCGCAGGGAGAGCCCCAGGTCCAGTTCAAACTTGTATTGGTTGGTGATGGTGGTACTGN
  GAAAAACGACCTTCGTGAAACGTCATTTGACTGGTGAATTTGAGAAGAAGTATGTAGCCA
  CCTTGGGTGTTGAGGTTCATCCCCTAGTGTTCCACACCAACAGAGGACCTATTAAGTTCA
  ATGTATGGGACACAGCCGGCCAGGAGAAATTCGGTGGACTGAGAGATGGCTATTATATCC
  AAGCCCAGTGTGCCATCATAATGTTTGATGTAACATCGAGAGTTACTTACAAGAATGTGC
  CTAACTGGCATAGAGATCTGGTACGAGTGTGTGAAAACATCCCCATTGTGTTGTGTGGCA
  ACAAAGTGGATATTAAGGACAGGAAAGTGAAGGCGAAATCCATTGTCTTCCACCGAAAGA
  AGAATCTTCAGTACTACGACATTTCTGCCAAAAGTAACTACAACTTTGAAAAGCCCTTCC
  TCTGGCTTGCTAGGAAGCTCATTGGAGACCCTAACTTGGAATTTGTTGCCATGCCTGCTC
  TCGCCCCACCAGAAGTTGTCATGGACCCAGCTTTGGCAGCACAGTATGAGCACGACTTAG
  AGGTTGCTCAGACNACTGCTCTCCCGGATGAAGATGATGACCTGTGAGAATGAAGCTGGA
  GCCCAGCGTCAGAAGTCTAGTTTTATAGGCAGCTGTCCTGTGATGTCAGCGGTGCAGCGT
  GTGTGCCACCTCATTATTATCTAGCTAAGCGAACATGTGCTTCATCTGTGGGATGCTGAA
  AGAGATGAGTGGGCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006325 unedited
  ACTTCCAGGGCCGGNATAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTC
  AGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGNNNTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTATTGGAAATACAACTTTATTCTGATTCTAAACGAAAAGGA
  ATGGGAATGACAGTAACAAACAAGATTTCACCACTGAATATTGGGATGTGACTGCAGCAG
  TCTTTTTTTTGAAACTCAAGGAATCAACTGCGTTCCAAAACAGCTAAATTTGCAGGTCCA
  AACAATGAAGTTATTTTTTAAACTGCCACATTCACTCCGAAGCCCACTCATTTTCTTTAG
  CATCCCACAGATGAAGCACATGTTCCGCTTAGCTAAATAATAATGAGGGGGCACACACCC
  TGNNNCGCTGACATCACAGGACAGCTGCCTATAAAACTAGACTTCTGACGCTGGGCTCCA
  GCTTCATTCTCACAGGTCATCATCCTCATCCGGGAGAGCAGTTGTCTGAGCAACCTCTAA
  GTCGTGCTCATACTGTGCTGCCAAAGCTGGGTCCATGACAACTTCTGGTGGGGGCGAGAG
  CAGGCATGGCAACAAATTCCAAGTTAGGGGTCTCCAATGANGCTTCCTAGCAAGCCAGAG
  GAAGAGGCTTTNCANNAGTTGTAGTTTACTTTTTGGCAGATATGNNTCGTAGTACTGAAG
  ATTCTTCTTTTCGGTGGAAGACAATGGATTNTCGCCTTCTNNNNNCCTGTCCTTTATATT
  CCACTTTTGTTGCCCACAACACATGGNGGAATGTTTTCACCCACTCGTACCAGATCTCTA
  TGCCAGTATGCACATTCTTGAAGTACTCTNGATGTTCATCAAACTATGATGGCACACTGG
  CTTGATATATAGCCTCTTTCAGCCACGATTTTTCTGGNCGGCTGTGNCCATCATTGACTT
  ATTAGGCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006325
• Insert Size: 1090 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: no
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006325.2, NP_006316.1
• RefSeq Size: 1656 bp
• RefSeq ORF: 651 bp
• Locus ID: 5901
• Cytogenetics: 12q24.33
• Domains: ras, RAN, RAS, RHO, RAB
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.