IRF6 (NM_006147) Human Untagged Clone

CAT#: SC116274

IRF6 (untagged)-Human interferon regulatory factor 6 (IRF6), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IRF6
• Synonyms: LPS; OFC6; PIT; PPS; PPS1; VWS; VWS1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116274 sequence for NM_006147 edited (data generated by NextGen Sequencing)
  ATGGCCCTCCACCCCCGCAGAGTCCGGCTAAAGCCCTGGCTGGTGGCCCAGGTGGATAGT
  GGCCTCTACCCTGGGCTCATCTGGCTACACAGGGACTCTAAACGCTTCCAGATTCCCTGG
  AAACATGCCACCCGGCATAGCCCTCAACAAGAAGAGGAAAATACCATTTTTAAGGCCTGG
  GCTGTAGAGACAGGGAAGTACCAGGAAGGGGTGGATGACCCTGACCCAGCTAAATGGAAG
  GCCCAGCTGCGCTGTGCTCTCAATAAGAGCAGAGAATTCAACCTGATGTATGATGGCACC
  AAGGAGGTGCCCATGAACCCAGTGAAGATATATCAAGTGTGTGACATCCCTCAGCCCCAG
  GGCTCGATCATTAACCCAGGATCCACAGGGTCTGCTCCCTGGGATGAGAAGGATAATGAT
  GTGGATGAAGAAGATGAGGAAGATGAGCTGGATCAGTCGCAGCACCATGTTCCCATCCAG
  GACACCTTCCCCTTCCTGAACATCAATGGTTCTCCCATGGCGCCAGCCAGTGTGGGCAAT
  TGCAGTGTGGGCAACTGCAGCCCGGAGGCAGTGTGGCCCAAAACTGAACCCCTGGAGATG
  GAAGTACCCCAGGCACCTATACAGCCCTTCTATAGCTCTCCAGAACTGTGGATCAGCTCT
  CTCCCAATGACTGACCTGGACATCAAGTTTCAGTACCGTGGGAAGGAGTACGGGCAGACC
  ATGACCGTGAGCAACCCTCAGGGCTGCCGACTCTTCTATGGGGACCTGGGTCCCATGCCT
  GACCAGGAGGAGCTCTTTGGTCCCGTCAGCCTGGAGCAGGTCAAATTCCCAGGTCCTGAG
  CATATTACCAATGAGAAGCAGAAGCTGTTCACTAGCAAGCTGCTGGACGTCATGGACAGA
  GGACTGATCCTGGAGGTCAGCGGTCATGCCATTTATGCCATCAGGCTGTGCCAGTGCAAG
  GTGTACTGGTCTGGGCCATGTGCCCCATCACTTGTTGCTCCCAACCTGATTGAGAGACAA
  AAGAAGGTCAAGCTATTTTGTCTGGAAACATTCCTTAGCGATCTCATTGCCCACCAGAAA
  GGACAGATAGAGAAGCAGCCACCGTTTGAGATCTACTTATGCTTTGGGGAAGAATGGCCA
  GATGGGAAACCATTGGAAAGGAAACTCATCTTGGTTCAGGTCATTCCAGTAGTGGCTCGG
  ATGATCTACGAGATGTTTTCTGGTGATTTCACACGATCCTTTGATAGTGGCAGTGTCCGC
  CTGCAGATCTCAACCCCAGACATCAAGGATAACATCGTTGCTCAGCTGAAGCAGCTGTAC
  CGCATCCTTCAAACCCAGGAGAGCTGGCAGCCCATGCAGCCCACCCCCAGCATGCAACTG
  CCCCCTGCCCTGCCTCCCCAGTAA
  
  Clone variation with respect to NM_006147.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006147 unedited
  TTCCCGCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAAGGTCTATATAAGCA
  GAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAA
  TTCGGCACCAGGCGCACCTGGGCTGGGCAGGTAAGGGCTGGTGCGGGACGGGGAGAGGAA
  CCTGCAGTCCCTACTTGGGTAGAGCCAGGCGCCCCTTGGCTAAGACGTCGAGGAGCGTGG
  TAGCGACGGGTGATCTTCGCTGCGGACTTGGTTCGGAGGGACGTCCGCTTCTGGTGGACA
  GATTGAGCAAAGAATCTTTGAGCGGTCAAGGGAAAGACAAGCCGACTCTTCAGATCCCTG
  TGGACACACTGCCTGCTCTTCCATATCATGGCCCTCCACCCCCGCAGAGTCCGGCTAAAG
  CCCTGGCTGGTGGCCCAGGTGGATAGTGGCCTCTACCCTGGGCTCATCTGGCTACACAGG
  GACTCTAAACGCTTCCAGATTCCCTGGAAACATGCCACCCGGCATAGCCCTCAACAAGAA
  GAGGAAAATACCATTTTTAAGGCCTGGGCTGTAGAGACAGGGAAGTACCAGGAAGGGGTG
  GATGACCCTGACCCAGCTAAATGGAATGCCCAGCTGCGCTGTGCTCTCAATAAGAGCAGA
  GAATTCAACCTGATGTATGATGGCACCAATGATGTGCCCATGAACCCAGTGAAGATATAT
  CAAGTGTGTGACATCCCTCAGCCCCAGGGCTCGATCATTAACCCANGATCCACAGGNTCT
  GCTCCNTGGGATGAGAAAGGATATGATGTGGATGAAGAATNATGAGGAGATGAGCTGGAT
  CAGTCGCAGCACCATGGTTCCATTCCAGACACCTNCCCTTNNCTGACAATCATGGNNTCT
  CCATGGCGCCAGCCANTGTGGGCAATTGCAGTGTGGGCAACTGCAGCCCCGNAGCAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006147 unedited
  NNGGGGTTTTGACTTTGGACCGGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTT
  TTCCTAAATTAAATCAGCTTTAAATCTAGGCATATTTGGGAAAATCACAAACTTCTAACA
  CTGTTAGAGAAAAGAGAGATTTAAAAGCTGGTTAAATCTAAACAATAAAAAAATCCATAT
  GTACAATATTATAAAAAAGAGAAGGAAGAAGATGGCATTCACAATTACTGGGGAGGCAGG
  GCAGGGGGCAGTTGCATGCTGGGGGTGGGCTGCATGGGCTGCCAGCTCTCCTGGGTTTGA
  AGGATGCGGTACAGCTGCTTCAGCTGAGCAACGATGTTATCCTTGATGTCTGGGGTTGAG
  ATCTGCAGGCGGACACTGCCACTATCAAAGGATCGTGTGAAATCACCAGAAAACATCTCG
  TAGATCATCCGAGCCACTACTGGAATGACCTGAACCAAGATGAGTTTCCTTTCCAATGGT
  TTCCCATCTGGCCATTCTTCCCCAAAGCATAAGTAGATCTCAAACGGTGGCTGCTTCTCT
  ATCTGTCCTTTCTGGTGGGCAATGAGATCGCTAAGGAATGTTTCCAGACAAAATAGCTTG
  ACCTTCTTTTGTCTCTCAATCAGGTTGGGAGCAACAAGTGATGGGGCACATGGCCCAGAC
  CAGTACACCTTGCACTGGCACAGCCTGATGGCATAAATGGCATGACCGCTGACCTCCAGG
  ATCAGTCCTCTGTCCATGACGTCCAGCAGCTTGCTAGTGAACAGCTTCTGCTTCTCATTG
  GTAATATGCTCAGGACCTGGGAATTTGACCTGCTCCAGGCTGACGGGGACCAAAGAGCTC
  TCCTGGTCAGGCATGGGACCCCAGTCCNCATAGAAAGAGTCGCAGCCCTGGAGGGTGCTC
  ACGTNCATGGTCTGCCCGTACTCTTCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006147
• Insert Size: 2100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006147.2, NP_006138.1
• RefSeq Size: 2171 bp
• RefSeq ORF: 1404 bp
• Locus ID: 3664
• Cytogenetics: 1q32.2
• Domains: IRF
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• Gene Summary: 'This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. The encoded protein may be a transcriptional activator. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. Mutations in this gene are also associated with non-syndromic orofacial cleft type 6. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2011]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.