MEF2B (BORCS8-MEF2B) (NM_005919) Human Untagged Clone

CAT#: SC116449

MEF2BNB (untagged)-ORIGENE UNIQUE VARIANT 1 of Human MEF2BNB-MEF2B readthrough (MEF2BNB-MEF2B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BORCS8-MEF2B
• Synonyms: LOC729991-MEF2B; MEF2B; MEF2BNB-MEF2B; RSRFR2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005919, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGGAGGAAAAAAATCCAGATCTCCCGCATCCTGGACCAAAGGAATCGGCAGGTGACGTTCACCAAGC
  GGAAGTTCGGGCTGATGAAGAAGGCCTATGAGCTGAGCGTGCTCTGTGACTGTGAGATAGCCCTCATCAT
  CTTCAACAGCGCCAACCGCCTCTTCCAGTATGCCAGCACGGACATGGACCGTGTGCTGCTGAAGTACACA
  GAGTACAGCGAGCCCCACGAGAGCCGCACCAACACTGACATCCTCGAGACGCTGAAGCGGAGGGGCATTG
  GCCTCGATGGGCCAGAGCTGGAGCCGGATGAAGGGCCTGAGGAGCCAGGAGAGAAGTTTCGGAGGCTGGC
  AGGCGAAGGGGGTGATCCGGCCTTGCCCCGACCCCGGCTGTATCCTGCAGCTCCTGCTATGCCCAGCCCA
  GATGTGGTATACGGGGCCTTACCGCCACCAGGCTGTGACCCCAGTGGGCTTGGGGAAGCACTGCCCGCCC
  AGAGCCGCCCATCTCCCTTCCGACCAGCAGCCCCCAAAGCCGGGCCCCCAGGCCTGGTGCACCCTCTCTT
  CTCACCAAGCCACCTCACCAGCAAGACACCACCCCCACTGTACCTGCCGACGGAAGGGCGGAGGTCAGAC
  CTGCCTGGTGGCCTGGCTGGGCCCCGAGGGGGACTAAACACCTCCAGAAGCCTCTACAGTGGCCTGCAGA
  ACCCCTGCTCCACTGCAACTCCCGGACCCCCACTGGGGAGCTTCCCCTTCCTCCCCGGAGGCCCCCCAGT
  GGGGGCCGAAGCCTGGGCGAGGAGGGTCCCCCAACCCGCGGCGCCTCCCCGCCGACCCCCCCAGTCAGCA
  TCAAGTCTGAGCGCCTCTCTCCGGCCCCCGGGGGCCCCGGCGACTTTCCTAAGACCTTCCCCTATCCCTT
  GCTCCTCGCCCGGTCCCTGGCAGAGCCTCTGCGGCCTGGGCCCGCCCTGCGCCGGCTGCCCTTGGCCGAC
  GGCTGGCCCCGGTAGGAGATCACCCGGTGGCACCAGCCCAGAGCGCTCGCCAGGTACGGCGAGGGCACGT
  GGGGACCCCACCTCCCTCCAGGCCTCTTCAGAGAAGACCCAACAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005919 unedited
  TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCTATGGAGGAGCCGGA
  GATGCAGCTCAAGGGGGAAGAAAGTCACGGACAGGTTCACTGAGAGCGTCTACGTCCTGG
  CCAACGAGCCATCCGTGGCCCTGTACCGGCTGCAGGAGCATGTGCGTCGCTCCCTCCCCG
  AGCTGGCCCAGCACAAGGCAGACATGCAGCGTTGGGAGGAGCAGAGCCAGGGAGCCATCT
  ACACTGTGGAGTACGCCTGCAGCGCCGTGAAGAACCTGGTGGACAGCAGCGTCTACTTCC
  GCAGCGTGGAGGGTCTGCTCAAACAGGCCATCAGCATCCGGGACCATATGAATGCCAGTG
  CCCAGGGCCACAGCCCGGAGGAACCACCCCCGCCCTCCTCAGCCTGATCCTGGAAGAGAC
  TCGGGGCCCCCCAGCCTCCGCCAACCCAGAGACGGGGTTTCACCATGTTGGCTAGGCTGG
  TCTCAAACTCCTGAGCTCGTGATCCTCCCGCCTCAGCCTCCCAAAGTGCTGGGATTATAG
  GTGTGAGCCACCGCACCCAGCCTTGGTTCTCCCTTTCTTCATCTGGATAAGGAAGGCTGG
  ACTGCACCTTCTCTACAGGCTGAGTTGGACATTCGCAGATCTACCCTCCCAACCTGCATT
  CTGCCTCAAGCAGCAACGCCCACCCCAGGCTCANGCCACATGGAGAAGCCACCCCTAGNT
  CCAGTGGTGGATGTGGCACCTCGGCCTGGCCATCAGAGCTCCCTTGTCTAGTTCCCTATG
  ATTGGTTNGAGATGGACACGTGACCCATGAGCGACCTTCCTGGGAGTTCTCCAGGAACAC
  AGGAAGGTGAGCTGGGTGTATGGGAACAGGCCCGGCCAGGGGGTCCGGGGGACCTGAAAA
  CATGGGGAGCCCTGGATCACTGTACTGAACTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005919 unedited
  GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCATGAAACTGAAGAAATGAAAT
  TGGCTAGTGTCAGGTACAGTTGGATCCAGGGCTCCACACAATGTTCTCAGGTCCCCCCGG
  AACCCCTCGCCCCGGCCTGGTTCCCATACACCCAGCTCAACCTTCCTGATGCTCCTGGTA
  GAACTCCCAGGAAAGGTCGCTCATTGGGTCACGTGTCCATCTCAAACCAATCACTAGGAA
  CCTAGGACAAGGGAGCTCTGATTGGCCAGGCCGAGGTGCCACATCCACCACTGGAACTAG
  GGGTGGCTTCTCCATGTGGCCTGAGCCTGGGGTGGGCGTTGCTGCTTGAGGCAGAATGCA
  GGTTGGGAGGGTAGATCTGCGAATGTCCAACTCAGCCTGTAGAGAAGGTGCAGTCCAGCC
  TTCCTTATCCAGATGAAGAAAGGGAGAACCAAGGCTGGGTGCGGTGGCTCACACCTATAA
  TCCCAGCACTTTGGGAGGCTGAGGCGGGAGGATCACGAGCTCAGGAGTTTGAGACCAGCC
  TAGCCAACATGGTGAAACCCCGTCTCTGGGTTGGCGGAGGCTGGGGGGCCCCGAGTCTCT
  TCCAGGATCAGGCTGAGGAGGGCGGGGGTGGTTCCTCCGGGCTGTGGCCCTNGGCACTGG
  CATTCATATGGTCCCGGATGCTGATGGCCTGTTTGAGCAGACCCTCCACGCTGCGGAAGT
  AGACGCTGCTGTCCACCANGTTCTTCACGGCGCTGCAGGCGTACTCCACAGTGTAGATGG
  CTCCCTGGCTCTGCTCCTCCAACGCTGCATGTNTGCCTTGTGCTGGGCCAGCTCGGGGAA
  GGAGCGACCCCCATGCTCCTGCANCCGTACAGGGCCACGNATGGGCTCGTGCCCAAGCCT
  AAACCCTCTCATGAAACTGCNCGGGACTTTCTCCCCTTGACTGATTTCCGGCTCTCCTAG
  CGCCTTGTGCCGAATCCCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005919
• Insert Size: 1050 bp
• OTI Disclaimer: The sequence of an 'OriGene Unique Variant' differs significantly from the associated reference. It represents a novel splice variant from the same gene locus of the reference. Although such variants are true transcripts and present opportunity for discoveries, they are not yet curated by NCBI and should not be used if the exact reference accession sequence is required.
• OTI Annotation: This TrueClone was found to represent an alternative form of the specific reference to which it is associated. Its Open Reading Frame (ORF) may represent a novel form or alternative splice variant. By virtue of it being a true transcript (cDNA clone not PCR product), it provides a biologically relevant copy of its mRNA template. For more details, please evaluate the sequence information provided on this website or contact our customer care specialists.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005919.1, NP_005910.1
• RefSeq Size: 1671 bp
• RefSeq ORF: 1098 bp
• Locus ID: 4207
• Cytogenetics: 19p13.11
• Domains: MADS
• Protein Families: Transcription Factors
• Gene Summary: 'This gene represents numerous read-through transcripts that span GeneID:729991 and 100271849. Many read-through transcripts are predicted to be nonsense-mediated decay (NMD) candidates, and are thought to be non-coding. Some transcripts are predicted to be capable of translation reinitiation at a downstream AUG, resulting in expression of at least one isoform of myocyte enhancer factor 2B (MEF2B) from this read-through locus. At least one additional MEF2B variant and isoform can be expressed from a downstream promoter, and is annotated on GeneID:100271849. [provided by RefSeq, Oct 2010]'
  Transcript Variant: This variant (1) lacks two alternate exons in the 5' region and one alternate exon in the 3' region, compared to variant 2. This variant is thought to be protein coding because translation can reinitiate at the downstream AUG, resulting in expression of an isoform of MEF2B (geneID:100271849). Isoform b has a shorter and distinct C-terminus, compared to MEF2A isoform a (NP_001139257.1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.