SYBL1 (VAMP7) (NM_005638) Human Untagged Clone

CAT#: SC116604

VAMP7 (untagged)-Human vesicle-associated membrane protein 7 (VAMP7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VAMP7
• Synonyms: SYBL1; TI-VAMP; TIVAMP; VAMP-7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116604 sequence for NM_005638 edited (data generated by NextGen Sequencing)
  ATGGCGATTCTTTTTGCTGTTGTTGCCAGGGGGACCACTATCCTTGCCAAACATGCTTGG
  TGTGGAGGAAACTTCCTGGAGGTGACAGAGCAGATTCTGGCTAAGATACCTTCTGAAAAT
  AACAAACTAACGTACTCACATGGCAATTATTTGTTTCATTACATCTGCCAAGACAGGATT
  GTATATCTTTGTATCACTGATGATGATTTTGAACGTTCCCGAGCCTTTAATTTTCTGAAT
  GAGATAAAGAAGAGGTTCCAGACTACTTACGGTTCAAGAGCACAGACAGCACTTCCATAT
  GCCATGAATAGCGAGTTCTCAAGTGTCTTAGCTGCACAGCTGAAGCATCACTCTGAGAAT
  AAGGGCCTAGACAAAGTGATGGAGACTCAAGCCCAAGTGGATGAACTGAAAGGAATCATG
  GTCAGAAACATAGATCTGGTAGCTCAGCGAGGAGAAAGATTGGAATTATTGATTGACAAA
  ACAGAAAATCTTGTGGATTCTTCTGTCACCTTCAAAACTACCAGCAGAAATCTTGCTCGA
  GCCATGTGTATGAAGAACCTCAAGCTCACTATTATCATCATCATCGTATCAATTGTGTTC
  ATCTATATCATTGTTTCACCTCTCTGTGGTGGATTTACATGGCCAAGCTGTGTGAAGAAA
  TAG
  
  Clone variation with respect to NM_005638.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005638 unedited
  ACTCGGATTTGTAACCGACTTTATATAGGCGGCACCGCAATTCGCACGAGGGCGCTTCAT
  GCGGAAGTCAGCGGCGTCCGGTCCCAGCCTCCTCTGGGAGCGGGCAGTTGGCGACCCTGC
  ACTGACCCGCGTCCCTCCGTCCCGAGCCCGCGCGCCCTCAGAGGGTGCCCGGACAGACTG
  AAGCCATGGCGATTCTTTTTGCTGTTGTTGCCTTTGGGACCACTATCCTTGCCAAACATG
  CTTGGTGTGGAGGAAACTTCCTGGAGGTGACAGAGCAGATTCTGGCTAAGATACCTTCTG
  AAAATAACAAACTAACGTACTCACATGGCAATTATTTGTTTCATTACATCTGCCAAGACA
  GGATTGTATATCTTTGTATCACTGATGATGATTTTGAACGTTCCCGAGCCTTTAATTTTC
  TGAATGAGATAAAGAAGAGGTTCCAGACTACTTACGGTTCAAGAGCACAGACAGCACTTC
  CATATGCCATGAATAGCGAGTTCTCAAGTGTCTTAGCTGCACAGCTGAAGCATCACTCTG
  AGAATAAGGGCCTAGACAAAGTGATGGAGACTCAAGCCCAAGTGGATGAACTGAAAGGAA
  TCATGGTCAGAAACATAGATCTGGTAGCTCAGCGAGGAGAAAGATTGGAATTATTGATTG
  ANCAAACAGAAAATCTTGTGGATTCTTCTGTCACCTTCAAAACTACCAGCAGAAATCTTG
  CTCGAGCCATGTGTATGAAGAACCTCAAGCTCACTATTATCATCATCATCGTATCAATTG
  TGTTCATCTATATCATTGTTTCACCTCTCTGTGGTGGGATTACATGGCCAAGCTGTGTGA
  AGAAATANGAAAGAAGAGTTACCATTACCAGGGTATGAGAGAACAAGGAGTAAAAGCATC
  CATGTGACTCAGCCTTTCACTACTGACAGAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005638 unedited
  GGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTGTATTACTCTTAAATTTTATT
  AACATCTTCAGTTTGTGCGTCATTTAAAATGAGACATGTGCTTTAAAAAGCATTCTTATA
  CATAAATAGACCAAGGAACAGTTAGGTAATTGATCCCTAAAACATGCACATCAATTTTAT
  TCAGGTGTGTATAAGAAAGGGAAATAAGCTTTAAACCTTTTTCTTTGGATTAAAAACATT
  TGGAAATTATTCAGGAATGCCAAATGTTTTTCTGGAACAGATGTATTTTCCAATAGGAAA
  TACTGATGCAATTAAGAGGCATTAGTGTTGATAAAGAAGACTGGAAAAACGTTTGTGCTA
  TGCTAGATAAACAAGAAAAGAGTTCAAGTGGGCCTAAGATCTATGTCAAATAAATGAATC
  AGGTAGCATGAATTGAAAGGTTTGGATAGAAGAACAGGTACCATGAGCCAGATTATGGGA
  CACATATATGTTCAAGGCACATGACTAGGCTAAACAGGTGGCTAGATTCTACAGACTAAT
  TTGTTCATTCATTGAGAAAGTGTAAAATGTAATATAATTTCAATTTAATGGCACTTATTG
  ATAAATAAATGCAATTGGATCTAGGGTAGAAAATGTCTTCCTTTCAGATACACACCAGAA
  ATGCATACTAGATAACAGATGCCAGTAGCGATATGATTACAGTCCAATTNTCTTACACTG
  CAGTTAAATGGGTGNTAAACTGGTTTGNATTAATTCTATATGNCATACTGGCTATTCTCT
  TTCAAGTTCACAAAAGATTCATCAAACTAGGCAGATTAAGAATTATTTTACCCACAAAAT
  GCTCANAACTATATTCNACAGAATCAGCCCAACCCTGNATTGACTGCTGACCGTATTCGG
  TTGGGTTTTCCANAATGGAAACTTTTTCACACTACTCCTTGACAGCTANAGCTACATTCC
  CTGGGNTCCTTCTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005638
• Insert Size: 2630 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005638.3, NP_005629.1
• RefSeq Size: 2588 bp
• RefSeq ORF: 663 bp
• Locus ID: 6845
• Cytogenetics: Xq28 and Yq12
• Domains: synaptobrevin
• Protein Families: Transcription Factors, Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• Gene Summary: 'This gene encodes a transmembrane protein that is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family. The encoded protein localizes to late endosomes and lysosomes and is involved in the fusion of transport vesicles to their target membranes. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.