POU3F2 (NM_005604) Human Untagged Clone

CAT#: SC116625

POU3F2 (untagged)-Human POU class 3 homeobox 2 (POU3F2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POU3F2
• Synonyms: brn-2; BRN2; N-Oct3; oct-7; OCT7; OTF-7; OTF7; POUF3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005604, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGACCGCAGCGTCTAACCACTACAGCCTGCTCACCTCCAGCGCCTCCATCGTGCACGCCGAGCCGC
  CCGGCGGCATGCAGCAGGGCGCGGGGGGCTACCGCGAAGCGCAGAGCCTGGTGCAGGGCGACTACGGCGC
  TCTGCAGAGCAACGGACACCCGCTCAGCCACGCTCACCAGTGGATCACCGCGCTGTCCCACGGCGGCGGC
  GGCGGGGGCGGTGGCGGCGGCGGGGGGGGCGGGGGCGGCGGCGGGGGCGGCGGCGACGGCTCCCCGTGGT
  CCACCAGCCCCCTGGGCCAGCCGGACATCAAGCCCTCGGTGGTGGTGCAGCAGGGCGGCCGCGGAGACGA
  GCTGCACGGGCCAGGCGCCCTGCAGCAGCAGCATCAGCAGCAGCAACAGCAACAGCAGCAGCAACAGCAG
  CAACAGCAGCAGCAGCAGCAGCAACAGCGGCCGCCGCATCTGGTGCACCACGCCGCTAACCACCACCCGG
  GACCCGGGGCATGGCGGAGCGCGGCGGCTGCAGCGCACCTCCCACCCTCCATGGGAGCGTCCAACGGCGG
  CTTGCTCTACTCGCAGCCCAGCTTCACGGTGAACGGCATGCTGGGCGCCGGCGGGCAGCCGGCCGGTCTG
  CACCACCACGGCCTGCGGGACGCGCACGACGAGCCACACCATGCCGACCACCACCCGCACCCGCACTCGC
  ACCCACACCAGCAGCCGCCGCCCCCGCCGCCCCCGCAGGGTCCGCCTGGCCACCCAGGCGCGCACCACGA
  CCCGCACTCGGACGAGGACACGCCGACCTCGGACGACCTGGAGCAGTTCGCCAAGCAGTTCAAGCAGCGG
  CGGATCAAACTGGGATTTACCCAAGCGGACGTGGGGCTGGCTCTGGGCACCCTGTATGGCAACGTGTTCT
  CGCAGACCACCATCTGCAGGTTTGAGGCCCTGCAGCTGAGCTTCAAGAACATGTGCAAGCTGAAGCCTTT
  GTTGAACAAGTGGTTGGAGGAGGCGGACTCGTCCTCGGGCAGCCCCACGAGCATAGACAAGATCGCAGCG
  CAAGGGCGCAAGCGGAAAAAGCGGACCTCCATCGAGGTGAGCGTCAAGGGGGCTCTGGAGAGCCATTTCC
  TCAAATGCCCCAAGCCCTCGGCCCAGGAGATCACCTCCCTCGCGGACAGCTTACAGCTGGAGAAGGAGGT
  GGTGAGAGTTTGGTTTTGTAACAGGAGACAGAAAGAGAAAAGGATGACCCCTCCCGGAGGGACTCTGCCG
  GGCGCCGAGGATGTGTACGGGGGGAGTAGGGACACTCCACCACACCACGGGGTGCAGACGCCCGTCCAGT
  GA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005604 unedited
  NATTATGTNATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGAGAGGGGGAGC
  GCCGAGCTAGTCAGAGAGTGAGCGAGAGCGAGAAGGAGGGAGAGGAGGAGAAAGAGAGCG
  AGGGCGGGCGGGAGGCGGCGGCGGCGGCAGCAGCAGCAGTAATAGCAGGAGCAGCAACAG
  AAGGCGTCGGAGCGGGCGTCGGAGCTGCCCGCTGTGGGAGAGAGAGGAGACAGAAAGAGC
  GAGCGAGGAGAGGGAGCCCGAGGCGAAAAAGTAACTGTCAAATGCGCGGCTCCTTTAACC
  GGAGCGCTCAGTCCGGCTCCGAGAGTCATGGCGACCGCAGCGTCTAACCACTACAGCCTG
  CTCACCTCCAGCGCCTCCATCGTGCACGCCGAGCCGCCCGGCGGCATGCAGCAGGGCGCG
  GGGGGCTACCGCGAAGCGCAGAGCCTGGTGCAGGGCGACTACGGCGCTCTGCAGAGCAAC
  GGACACCCGCTCAGCCACGCTCACCAGTGGATCACCGCGCTGTCCCACGGCGGCGGCGGC
  GGGGGCGGTGGCGGCGGCGGGGGGGCCGGTCCTCTTTTCTCTCTCTATTCTCATCTCTTC
  CCGCATTTCCCACCCCCCCCCCCCCTCTATTTTTTCTCTCTTCCAGCGCCCCTCCTCCTC
  TCACTACCTTCTTCCCTCTCCCTCCTTTTCTTTTTTCCCCTTCACTTATTATAACACCCC
  TCTCCCCCCCTCCCTTCTAACTATTTCTCCCCTCCCTCTCTATCCTCGCACCCCTCCCCA
  CCCCTCCACTCGTATCTCCCTTCCTCTCGCTGTTATTTATTCCTTCTATTGTACAATCAT
  CTCTACTCTTCTATTCCTTTCTTTCATCTCCCACCCANTTTTACTCTTGGTTCCGCCCAC
  GCCCCCTCGCTCTCCTTCCTCTCTTATTTCTCCCTCTACTCCCCACCTATCTCTTCCTTC
  CCCGCTTATCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005604 unedited
  TATGGACCGCGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATAAGATCTCT
  GCGTTTTTATTTTCCCCACAAGCACAACCAAATGTACCAAAACAAAGTATTTTTTAAGAG
  ACTGAACAAAAAAATTACATCCCATATAGATTTTGCTCACCTATTCATTTAAAGCTACAG
  AAAAACAGTTTCCAGAACCTTTTTGTTTCAAAAGAAATAAATATACATTGAGGCAGGCCA
  CTTAAAAATGATATGAAAATATTTCCCTGGGTAGCATTAAAAAAAAAGAAAAATAGACAA
  AGAAACATTTNNAAACTATTTTNCTGGCATTCTTCAAAAGACAGATATCTAACATATATA
  CATAAGTGGTAAAGGTTCAAGTGGGTAACTTTTCATTTCTTAGTAAACCCTATTNAGAGC
  CAGGAGAAAGCTGTTAGACACATTTCTCTTGGAACTCCCAACCTTTTTGGAACCTTTACC
  CTTAACTTGGGGGAATAAAAGGATATTCTGGGGGAAAATTCAATTTTCCCTCCCCTTTAA
  CCAAAGGGGTATTTTTGGCCCCTGCGCTTGGGGAAAAGCCTTCCCCTTAAAGGGGCGCCA
  TTTCCCCCTTCCCCCCTTTGGGNNNNNNNAAGCCNAACGGGTNTNCCACCCCGGAAANAN
  NNTCTCCNGGGGCCACATTTTCCTTTGGGGAGACATTTTTTGTGGGGGGGGAAAGGGAAA
  AAACCCGCGCGGAATTAATATTTGGCCCCCCTTATAAAGGGAACCCCCTTTTTTTCTTGG
  GGCGAAAACAGCGGGAGAGAGGAAGAAACCCCCCCGGGGTCGTGTGAGTAAGAAGCATAT
  TTTTAGGGCTTTTTTTTTCTCTAAAACCCTTTAAAGAACCTCGCGGGGGGGGAGCGCTGA
  TTNTGTGGTCGCGGCACCCACAATTTTTTGGTGGGGGGGGGGACTCTCTCTTTTTCTTTA
  TATAAAAAAAAATATCTCTTTCCCCCCCCCCGGGCGGGAAAAAACCCCCCCCCCTCTTCT
  TTTAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005604
• Insert Size: 3410 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: A trueClone.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005604.2, NP_005595.2
• RefSeq Size: 4108 bp
• RefSeq ORF: 1332 bp
• Locus ID: 5454
• Cytogenetics: 6q16.1
• Domains: POU, homeobox
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a member of the POU-III class of neural transcription factors. The encoded protein is involved in neuronal differentiation and enhances the activation of corticotropin-releasing hormone regulated genes. Overexpression of this protein is associated with an increase in the proliferation of melanoma cells. [provided by RefSeq, Mar 2012]'