RHEB (NM_005614) Human Untagged Clone

CAT#: SC116636

RHEB (untagged)-Human Ras homolog enriched in brain (RHEB)


  "NM_005614" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol RHEB
Synonyms RHEB2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_005614 edited
GAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGGCCGCCGCGGTTGATGTGGTTG
GGCCGGGGCTGAGGAGGCCGCCAAGATGCCGCAGTCCAAGTCCCGGAAGATCGCGATCCT
GGGCTACCGGTCTGTGGGGAAATCCTCATTGACGATTCAATTTGTTGAAGGCCAATTTGT
GGACTCCTACGATCCAACCATAGAAAACACTTTTACAAAGTTGATCACAGTAAATGGACA
AGAATATCATCTTCAACTTGTAGACACAGCCGGGCAAGATGAATATTCTATCTTTCCTCA
GACATACTCCATAGATATTAATGGCTATATTCTTGTGTATTCTGTTACATCAATCAAAAG
TTTTGAAGTGATTAAAGTTATCCATGGCAAATTGTTGGATATGGTGGGGAAAGTACAAAT
ACCTATTATGTTGGTTGGGAATAAGAAAGACCTGCATATGGAAAGGGTGATCAGTTATGA
AGAAGGGAAAGCTTTGGCAGAATCTTGGAATGCAGCTTTTTTGGAATCTTCTGCTAAAGA
AAATCAGACTGCTGTGGATGTTTTTCGAAGGATAATTTTGGAGGCAGAAAAAATGGACGG
GGCAGCTTCACAAGGCAAGTCTTCATGCTCGGTGATGTGATTCTGCTGCAAAGCCTGAGG
ACACTGGGAATATATTCTACCTGAAGAAGCAAACTGCCCGTTCTCCTTGAAGATAAACTA
TGCTTCTTTTTTCTTCTGTTAACCTGAAAGATATCATTTGGGTCAGAGCTCCCCTCCCTT
CAGATTATGTTAACTCTGAGTCTGTCCAAATGAGTTCACTTCCATTTTCAAATTTTAAGC
AATCATATTTTCAATTTATATATTGTATTTCTTAATATTATGACCAAGAATTTTATCGGC
ATTAATTTTTCAGTGTAGTTTGTTGTTTAAAATAATGTAATCATCAAAATGATGCATATT
GTTACACTACTATTAACTAGGCTTCAGTATATCAGTGTTTATTTCATTGTGTTAAATGTA
TACTTGTAAATAAAATAGCTGCAAACCTCAAAAAAAAAAAAAAAAAACTCGAC
>OriGene 5' read for NM_005614 unedited
TTTTGTAATACGACTCACTATAGGGCGGCACGCGAATTCGCACGAGGCCTCGTGCCGAAT
TCGGCACGAGGCCGCCGCGGTTGATGTGGTTGGGCCGGGGCTGAGGAGGCCGCCAAGATG
CCGCAGTCCAAGTCCCGGAAGATCGCGATCCTGGGCTACCGGTCTGTGGGGAAATCCTCA
TTGACGATTCAATTTGTTGAAGGCCAATTTGTGGACTCCTACGATCCAACCATAGAAAAC
ACTTTTACAAAGTTGATCACAGTAAATGGACAAGAATATCATCTTCAACTTGTAGACACA
GCCGGGCAAGATGAATATTCTATCTTTCCTCAGACATACTCCATAGATATTAATGGCTAT
ATTCTTGTGTATTCTGTTACATCAATCAAAAGTTTTGAAGTGATTAAAGTTATCCATGGC
AAATTGTTGGATATGGTGGGGAAAGTACAAATACCTATTATGTTGGTTGGGAATAAGAAA
GACCTGCATATGGAAAGGGTGATCAGTTATGAAGAAGGGAAAGCTTTGGCAGAATCTTGG
AATGCAGCTTTTTTGGAATCTTCTGCTAAAGAAAATCAGACTGCTGTGGATGTTTTTCGA
AGGATAATTTTGGAGGCAGAAAAAATGGACGGNGCAGCTTCACAAGGCAAGTCTTCATGC
TCGGTGATGTGATTCTGCTGCAAAGCCTGAGGACACTGNGAATATATTCTACCTGAAGAA
GCANACTGCCCGTTCTCCTTGAAGATAACTATGCTTCTTTNTTCTTCTGTTAACCTGAAA
GAATCATTTGGGTCAGAGCTCCCCTTCCCTTCAGATATGTACCTCTGAGTCTGTCCAAAT
GAGTCACTTCATTNTCAATTTAAGCATCATATTTCATTTATTATGGNATTCTTATATATG
ACAAGATNTAT
>OriGene 3' read for NM_005614 unedited
GGGGGCGGGGGNNGGTGTTNNGGTTCTGTGTGCCGNNTGCCGTTCCCCGACTCTGCCCCC
GCGGCCCGCCCACTGACGATCCGTTTTTTTTTTTTTTTTTTGCAGTTTGCAGCTATTTTA
TTTACAAGCATACATTTAACACAATGCAAATAAACACTGATATACTGAAGCCTAGTTAAT
AGTAGTGTAACAATATGCATCATTTTGATGATTACATTATTTTAAACAACAAACTACACT
GGAAAAATTAATGCCGATAAAATTCTTGGCCATAATATTAAGAAATACCATATATAACTT
GAAAATATGATTGCTTAAAATTTGAAAATGGAAGTGAACTCATTTGGACAGACTCAGAGT
TAACATAATCTGAATGGAGGGGAGCTCTGACCCAAATGATATCTTTCAGGTTAACAGAAG
AAAAAAGAAGCATAGCTTATTTTCAAGGAGAACGGGCAGTTTGCTTTTTCAGGTAGAATA
TATTCCCAGTGTCCTCACGCTTTGCAGCAGAATCACATCACCGAGCATGAACACTTGCCT
TGTGAAGCTGCCCCGTCCATTTTTTCTGCCTCCAAAAATATCCCTCGAAAAACCATCCAC
AGCACCCTGATTTTTTTTAGCCCAAACACTCCCAAAAAGCTGCATTCCCCGCATTCTGCC
AACACCTTTCCCTTCCTCCATACCTGAATCCCCCTTTCCATAATGCAGGGCCTTTCCTTA
TTTCCAACCCCACCATATTAGGCTATTTTGTACTTTTTCCCCCACCATATCCAACCCAAT
CTGCCCCTTGGGAAAACCTTTCAATCCACTTCCCAAACTTTTTGATCTGATGTCACCAGC
ACTTCCCCACGGAATATAACCCCTTCAACAACCCAAGGGAGCAAGGTTTCGAAGGCAAAC
AAAGCAAAATTTCATCCTTGGCCCGGGCCGGGGTTCTAAAAAATTCGAACATGAATACTC
T
Restriction Sites NotI-NotI     
ACCN NM_005614
Insert Size 1000 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005614.2, NP_005605.1
RefSeq Size 1396 bp
RefSeq ORF 555 bp
Locus ID 6009
Cytogenetics 7q36.1
Domains ras, RAS, RHO, RAB
Protein Pathways Insulin signaling pathway, mTOR signaling pathway
Gene Summary 'This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008]'

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.