RHEB (NM_005614) Human Untagged Clone

CAT#: SC116636

RHEB (untagged)-Human Ras homolog enriched in brain (RHEB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RHEB
• Synonyms: RHEB2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005614 edited
  GAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGGCCGCCGCGGTTGATGTGGTTG
  GGCCGGGGCTGAGGAGGCCGCCAAGATGCCGCAGTCCAAGTCCCGGAAGATCGCGATCCT
  GGGCTACCGGTCTGTGGGGAAATCCTCATTGACGATTCAATTTGTTGAAGGCCAATTTGT
  GGACTCCTACGATCCAACCATAGAAAACACTTTTACAAAGTTGATCACAGTAAATGGACA
  AGAATATCATCTTCAACTTGTAGACACAGCCGGGCAAGATGAATATTCTATCTTTCCTCA
  GACATACTCCATAGATATTAATGGCTATATTCTTGTGTATTCTGTTACATCAATCAAAAG
  TTTTGAAGTGATTAAAGTTATCCATGGCAAATTGTTGGATATGGTGGGGAAAGTACAAAT
  ACCTATTATGTTGGTTGGGAATAAGAAAGACCTGCATATGGAAAGGGTGATCAGTTATGA
  AGAAGGGAAAGCTTTGGCAGAATCTTGGAATGCAGCTTTTTTGGAATCTTCTGCTAAAGA
  AAATCAGACTGCTGTGGATGTTTTTCGAAGGATAATTTTGGAGGCAGAAAAAATGGACGG
  GGCAGCTTCACAAGGCAAGTCTTCATGCTCGGTGATGTGATTCTGCTGCAAAGCCTGAGG
  ACACTGGGAATATATTCTACCTGAAGAAGCAAACTGCCCGTTCTCCTTGAAGATAAACTA
  TGCTTCTTTTTTCTTCTGTTAACCTGAAAGATATCATTTGGGTCAGAGCTCCCCTCCCTT
  CAGATTATGTTAACTCTGAGTCTGTCCAAATGAGTTCACTTCCATTTTCAAATTTTAAGC
  AATCATATTTTCAATTTATATATTGTATTTCTTAATATTATGACCAAGAATTTTATCGGC
  ATTAATTTTTCAGTGTAGTTTGTTGTTTAAAATAATGTAATCATCAAAATGATGCATATT
  GTTACACTACTATTAACTAGGCTTCAGTATATCAGTGTTTATTTCATTGTGTTAAATGTA
  TACTTGTAAATAAAATAGCTGCAAACCTCAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005614 unedited
  TTTTGTAATACGACTCACTATAGGGCGGCACGCGAATTCGCACGAGGCCTCGTGCCGAAT
  TCGGCACGAGGCCGCCGCGGTTGATGTGGTTGGGCCGGGGCTGAGGAGGCCGCCAAGATG
  CCGCAGTCCAAGTCCCGGAAGATCGCGATCCTGGGCTACCGGTCTGTGGGGAAATCCTCA
  TTGACGATTCAATTTGTTGAAGGCCAATTTGTGGACTCCTACGATCCAACCATAGAAAAC
  ACTTTTACAAAGTTGATCACAGTAAATGGACAAGAATATCATCTTCAACTTGTAGACACA
  GCCGGGCAAGATGAATATTCTATCTTTCCTCAGACATACTCCATAGATATTAATGGCTAT
  ATTCTTGTGTATTCTGTTACATCAATCAAAAGTTTTGAAGTGATTAAAGTTATCCATGGC
  AAATTGTTGGATATGGTGGGGAAAGTACAAATACCTATTATGTTGGTTGGGAATAAGAAA
  GACCTGCATATGGAAAGGGTGATCAGTTATGAAGAAGGGAAAGCTTTGGCAGAATCTTGG
  AATGCAGCTTTTTTGGAATCTTCTGCTAAAGAAAATCAGACTGCTGTGGATGTTTTTCGA
  AGGATAATTTTGGAGGCAGAAAAAATGGACGGNGCAGCTTCACAAGGCAAGTCTTCATGC
  TCGGTGATGTGATTCTGCTGCAAAGCCTGAGGACACTGNGAATATATTCTACCTGAAGAA
  GCANACTGCCCGTTCTCCTTGAAGATAACTATGCTTCTTTNTTCTTCTGTTAACCTGAAA
  GAATCATTTGGGTCAGAGCTCCCCTTCCCTTCAGATATGTACCTCTGAGTCTGTCCAAAT
  GAGTCACTTCATTNTCAATTTAAGCATCATATTTCATTTATTATGGNATTCTTATATATG
  ACAAGATNTAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005614 unedited
  GGGGGCGGGGGNNGGTGTTNNGGTTCTGTGTGCCGNNTGCCGTTCCCCGACTCTGCCCCC
  GCGGCCCGCCCACTGACGATCCGTTTTTTTTTTTTTTTTTTGCAGTTTGCAGCTATTTTA
  TTTACAAGCATACATTTAACACAATGCAAATAAACACTGATATACTGAAGCCTAGTTAAT
  AGTAGTGTAACAATATGCATCATTTTGATGATTACATTATTTTAAACAACAAACTACACT
  GGAAAAATTAATGCCGATAAAATTCTTGGCCATAATATTAAGAAATACCATATATAACTT
  GAAAATATGATTGCTTAAAATTTGAAAATGGAAGTGAACTCATTTGGACAGACTCAGAGT
  TAACATAATCTGAATGGAGGGGAGCTCTGACCCAAATGATATCTTTCAGGTTAACAGAAG
  AAAAAAGAAGCATAGCTTATTTTCAAGGAGAACGGGCAGTTTGCTTTTTCAGGTAGAATA
  TATTCCCAGTGTCCTCACGCTTTGCAGCAGAATCACATCACCGAGCATGAACACTTGCCT
  TGTGAAGCTGCCCCGTCCATTTTTTCTGCCTCCAAAAATATCCCTCGAAAAACCATCCAC
  AGCACCCTGATTTTTTTTAGCCCAAACACTCCCAAAAAGCTGCATTCCCCGCATTCTGCC
  AACACCTTTCCCTTCCTCCATACCTGAATCCCCCTTTCCATAATGCAGGGCCTTTCCTTA
  TTTCCAACCCCACCATATTAGGCTATTTTGTACTTTTTCCCCCACCATATCCAACCCAAT
  CTGCCCCTTGGGAAAACCTTTCAATCCACTTCCCAAACTTTTTGATCTGATGTCACCAGC
  ACTTCCCCACGGAATATAACCCCTTCAACAACCCAAGGGAGCAAGGTTTCGAAGGCAAAC
  AAAGCAAAATTTCATCCTTGGCCCGGGCCGGGGTTCTAAAAAATTCGAACATGAATACTC
  T
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005614
• Insert Size: 1000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005614.2, NP_005605.1
• RefSeq Size: 1396 bp
• RefSeq ORF: 555 bp
• Locus ID: 6009
• Cytogenetics: 7q36.1
• Domains: ras, RAS, RHO, RAB
• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway
• Gene Summary: 'This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008]'