LIM kinase 2 (LIMK2) (NM_005569) Human Untagged Clone

CAT#: SC116659

LIMK2 (untagged)-Human LIM domain kinase 2 (LIMK2), transcript variant 2a

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LIMK2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005569 edited
  ATGTCCGCGCTGGCGGGTGAAGATGTCTGGAGGTGTCCAGGCTGTGGGGACCACATTGCT
  CCAAGCCAGATATGGTACAGGACTGTCAACGAAACCTGGCACGGCTCTTGCTTCCGGTGT
  TCAGAATGCCAGGATTCCCTCACCAACTGGTACTATGAGAAGGATGGGAAGCTCTACTGC
  CCCAAGGACTACTGGGGGAAGTTTGGGGAGTTCTGTCATGGGTGCTCCCTGCTGATGACA
  GGGCCTTTTATGGTGGCTGGGGAGTTCAAGTACCACCCAGAGTGCTTTGCCTGTATGAGC
  TGCAAGGTGATCATTGAGGATGGGGATGCATATGCACTGGTGCAGCATGCCACCCTCTAC
  TGTGGGAAGTGCCACAATGAGGTGGTGCTGGCACCCATGTTTGAGAGACTCTCCACAGAG
  TCTGTTCAGGAGCAGCTGCCCTACTCTGTCACGCTCATCTCCATGCCGGCCACCACTGAA
  GGCAGGCGGGGCTTCTCCGTGTCCGTGGAGAGTGCCTGCTCCAACTACGCCACCACTGTG
  CAAGTGAAAGAGGTCAACCGGATGCACATCAGTCCCAACAATCGAAACGCCATCCACCCT
  GGGGACCGCATCCTGGAGATCAATGGGACCCCCGTCCGCACACTTCGAGTGGAGGAGGTG
  GAGGATGCAATTAGCCAGACGAGCCAGACACTTCAGCTGTTGATTGAACATGACCCCGTC
  TCCCAACGCCTGGACCAGCTGCGGCTGGAGGCCCGGCTCGCTCCTCACATGCAGAATGCC
  GGACACCCCCACGCCCTCAGCACCCTGGACACCAAGGAGAATCTGGAGGGGACACTGAGG
  AGACGTTCCCTAAGGCGCAGTAACAGTATCTCCAAGTCCCCTGGCCCCAGCTCCCCAAAG
  GAGCCCCTGCTGTTCAGCCGTGACATCAGCCGCTCAGAATCCCTTCGTTGTTCCAGCAGC
  TATTCACAGCAGATCTTCCGGCCCTGTGACCTAATCCATGGGGAGGTCCTGGGGAAGGGC
  TTCTTTGGGCAGGCTATCAAGGTGACACACAAAGCCACGGGCAAAGTGATGGTCATGAAA
  GAGTTAATTCGATGTGATGAGGAGACCCAGAAAACTTTTCTGACTGAGGTGAAAGTGATG
  CGCAGCCTGGACCACCCCAATGTGCTCAAGTTCATTGGTGTGCTGTACAAGGATAAGAAG
  CTGAACCTGCTGACAGAGTACATTGAGGGGGGCACACTGAAGGACTTTCTGCGCAGTATG
  GATCCGTTCCCCTGGCAGCAGAAGGTCAGGTTTGCCAAAGGAATCGCCTCCGGAATGGCC
  TATTTGCACTCTATGTGCATCATCCACCGGGATCTGAACTCGCACAACTGCCTCATCAAG
  TTGGACAAGACTGTGGTGGTGGCAGACTTTGGGCTGTCACGGCTCATAGTGGAAGAGAGG
  AAAAGGGCCCCCATGGAGAAGGCCACCACCAAGAAACGCACCTTGCGCAAGAACGACCGC
  AAGAAGCGCTACACGGTGGTGGGAAACCCCTACTGGATGGCCCCTGAGATGCTGAACGGA
  AAGAGCTATGATGAGACGGTGGATATCTTCTCCTTTGGGATCGTTCTCTGTGAGATCATT
  GGGCAGGTGTATGCAGATCCTGACTGCCTTCCCCGAACACTGGACTTTGGCCTCAACGTG
  AAGCTTTTCTGGGAGAAGTTTGTTCCCACAGATTGTCCCCCGGCCTTCTTCCCGCTGGCC
  GCCATCTGCTGCAGACTGGAGCCTGAGAGCAGACCAGCATTCTCGAAATTGGAGGACTCC
  TTTGAGGCCCTCTCCCTGTACCTGGGGGAGCTGGGCATCCCGCTGCCTGCAGAGCTGGAG
  GAGTTGGACCACACTGTGAGCATGCAGTACGGCCTGACCCGGGACTCACCTCCCTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005569 unedited
  GTTCNCATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGAACTAAGGG
  AGCTGCTGTGTCCCCCGCCTCCTCCTCCCCATTTCCGCGCTCCCGGGACCATGTCCGCGC
  TGGCGGGTGAAGATGTCTGGAGGTGTCCAGGCTGTGGGGACCACATTGCTCCAAGCCAGA
  TATGGTACAGGACTGTCAACGAAACCTGGCACGGCTCTTGCTTCCGGTGTTCAGAATGCC
  AGGATTCCCTCACCAACTGGTACTATGAGAAGGATGGGAAGCTCTACTGCCCCAAGGACT
  ACTGGGGGAAGTTTGGGGAGTTCTGTCATGGGTGCTCCCTGCTGATGACAGGGCCTTTTA
  TGGTGGCTGGGGAGTTCAAGTACCACCCAGAGTGCTTTGCCTGTATGAGCTGCAAGGTGA
  TCATTGAGGATGGGGATGCATATGCACTGGTGCAGCATGCCACCCTCTACTGTGGGAAGT
  GCCACAATGAGGTGGTGCTGGCACCCATGTTTGAGAGACTCTCCACAGAGTCTGTTCAGG
  AGCAGCTGCCCTACTCTGTCACGCTCATCTCCATGCCGGCCACCACTGAAGGCAGGCGGG
  GCTTCTCCGTGTCCGTGGAGAGTGCCTGCTCCAACTACGCCACCACTGTGCAAGTGAAAG
  AGGTCAACCGGATGCACATCAGTCCCAACAATCGAAACGCCATCCACCCTTGGGACCGCA
  TCCTGGAGATCAATGGGACCCCCGTCCGCACACTTCNAGTGGANGAGGTGNGAGATGCAA
  TTAGCCAGACGAGCCAGACACTNTCAGCTGTGATTGAACATGACCCCGTCTCCAACGCCT
  GGACCAGCTGCGGCTGGNAGCCCNGCTCGCTNCTCACATGCAGAAATGCCGACACCCNCA
  CGCCCTCAGCACCCTGGACACAAGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005569 unedited
  NTTTTACTCTGGACCCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTTATG
  TTAACTGCTTAATTTATTTAGTCTGATCAATGGCCCAGTTCAGGCCCACCACTCCTACAC
  TTGCTGGGGACAGCCAGGAAACAACCAGCCACACAATGCTGAACTGTGCTGCTGCTGGGA
  GTCCAGGGCTCAGCCCTAAAGCAAGCTTGCAAACTTCACACATAAGTACAGTCTATATAG
  CAAGTAAACTCTGACCAGAGATGACATCTGGTCCCACAACTCATCAGGTCTATGTACAAT
  ATTTCACATACCACCCAATAGATAAGATAATATTAACAGCAACCACTCTCCTTTATCAAT
  TCCCCCTGCTCCAATACAACCACCACACATTGCATTAATACCCCAAACCCATTCCCAATT
  TATTAAATATGGTGCAAGCTCATAGACACTTAGAAGAGGCAAATCTAGTTGTGATGAAGA
  GTTCCTAGAGCTCTGGGAGCCAAGATGGAGGTTCCCCAGTACCTGCACATGTGGCTCAGG
  AGGATGCTGCCCAGGAGCTAATGAGTCGGGAGAGCAAACATGGGAGGTAGAAGTCAAATG
  GCCCAGCTCAGGGAGCTATCTCTCTCAGCATATCAGGTTTGAGACTCTGCCGACACCTGT
  TTCCAGCCCAAGCTGCTGCCTAAACCAACACTGCTGCAAGGTGACCCGTGGTTGCCTGAA
  TTCCTGACCACAGATGCTGGGACCCCCGCTGCGCCCGCTGAAACACCAGCGCCGCAACGA
  CCCCCGTGGCTCGTAGATCGCTGCACTTCCTCCAGACGTATCGAGCCGCCCGTACAGCCC
  GCGCCCCGACTGAACGATCCACCGACTATCCGCCTCTGTTAAGGCCCACCCCTTTAGAGA
  CGAGACCAGGACGGCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005569
• Insert Size: 4690 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005569.3, NP_005560.1
• RefSeq Size: 3701 bp
• RefSeq ORF: 1917 bp
• Locus ID: 3985
• Cytogenetics: 22q12.2
• Domains: pkinase, TyrKc, PDZ, LIM, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton
• Gene Summary: 'There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2a) differs in the 5' UTR and coding region as well as the 3' UTR and coding region, compared to variant 1. The resulting isoform (2a) is shorter and has distinct N- and C-termini compared to isoform 1.