LIM kinase 2 (LIMK2) (NM_005569) Human Untagged Clone

CAT#: SC323590

LIMK2 (untagged)-Kinase deficient mutant (K360M) of Human LIM domain kinase 2 (LIMK2), transcript variant 2a

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LIMK2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005569, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCCGCGCTGGCGGGTGAAGATGTCTGGAGGTGTCCAGGCTGTGGGGACCACATTGCTCCAAGCCAGA
  TATGGTACAGGACTGTCAACGAAACCTGGCACGGCTCTTGCTTCCGGTGTTCAGAATGCCAGGATTCCCT
  CACCAACTGGTACTATGAGAAGGATGGGAAGCTCTACTGCCCCAAGGACTACTGGGGGAAGTTTGGGGAG
  TTCTGTCATGGGTGCTCCCTGCTGATGACAGGGCCTTTTATGGTGGCTGGGGAGTTCAAGTACCACCCAG
  AGTGCTTTGCCTGTATGAGCTGCAAGGTGATCATTGAGGATGGGGATGCATATGCACTGGTGCAGCATGC
  CACCCTCTACTGTGGGAAGTGCCACAATGAGGTGGTGCTGGCACCCATGTTTGAGAGACTCTCCACAGAG
  TCTGTTCAGGAGCAGCTGCCCTACTCTGTCACGCTCATCTCCATGCCGGCCACCACTGAAGGCAGGCGGG
  GCTTCTCCGTGTCCGTGGAGAGTGCCTGCTCCAACTACGCCACCACTGTGCAAGTGAAAGAGGTCAACCG
  GATGCACATCAGTCCCAACAATCGAAACGCCATCCACCCTGGGGACCGCATCCTGGAGATCAATGGGACC
  CCCGTCCGCACACTTCGAGTGGAGGAGGTGGAGGATGCAATTAGCCAGACGAGCCAGACACTTCAGCTGT
  TGATTGAACATGACCCCGTCTCCCAACGCCTGGACCAGCTGCGGCTGGAGGCCCGGCTCGCTCCTCACAT
  GCAGAATGCCGGACACCCCCACGCCCTCAGCACCCTGGACACCAAGGAGAATCTGGAGGGGACACTGAGG
  AGACGTTCCCTAAGGCGCAGTAACAGTATCTCCAAGTCCCCTGGCCCCAGCTCCCCAAAGGAGCCCCTGC
  TGTTCAGCCGTGACATCAGCCGCTCAGAATCCCTTCGTTGTTCCAGCAGCTATTCACAGCAGATCTTCCG
  GCCCTGTGACCTAATCCATGGGGAGGTCCTGGGGAAGGGCTTCTTTGGGCAGGCTATCAAGGTGACACAC
  AAAGCCACGGGCAAAGTGATGGTCATGAAAGAGTTAATTCGATGTGATGAGGAGACCCAGAAAACTTTTC
  TGACTGAGGTGAAAGTGATGCGCAGCCTGGACCACCCCAATGTGCTCAAGTTCATTGGTGTGCTGTACAA
  GGATAAGAAGCTGAACCTCCTGACAGAGTACATTGAGGGGGGCACACTGAAGGACTTTCTGCGCAGTATG
  GATCCGTTCCCCTGGCAGCAGAAGGTCAGGTTTGCCAAAGGAATCGCCTCCGGAATGGCCTATTTGCACT
  CTATGTGCATCATCCACCGGGATCTGAACTCGCACAACTGCCTCATCAAGTTGGACAAGACTGTGGTGGT
  GGCAGACTTTGGGCTGTCACGGCTCATAGTGGAAGAGAGGAAAAGGGCCCCCATGGAGAAGGCCACCACC
  AAGAAACGCACCTTGCGCAAGAACGACCGCAAGAAGCGCTACACGGTGGTGGGAAACCCCTACTGGATGG
  CCCCTGAGATGCTGAACGGAAAGAGCTATGATGAGACGGTGGATATCTTCTCCTTTGGGATCGTTCTCTG
  TGAGATCATTGGGCAGGTGTATGCAGATCCTGACTGCCTTCCCCGAACACTGGACTTTGGCCTCAACGTG
  AAGCTTTTCTGGGAGAAGTTTGTTCCCACAGATTGTCCCCCGGCCTTCTTCCCGCTGGCCGCCATCTGCT
  GCAGACTGGAGCCTGAGAGCAGACCAGCATTCTCGAAATTGGAGGACTCCTTTGAGGCCCTCTCCCTGTA
  CCTGGGGGAGCTGGGCATCCCGCTGCCTGCAGAGCTGGAGGAGTTGGACCACACTGTGAGCATGCAGTAC
  GGCCTGACCCGGGACTCACCTCCCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_005569 unedited
  ACCGCCCGTATCAGCAATGGGCGGTAGGCGCTGTACGGATGGGAGGTCTATATAAGCAGAGCTCGTTTAG
  TGAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAACTAAGGG
  GAGCTGCTGTGTCCCCCGCCTCCTCCTCCCCATTTCCGCGCTCCCGGGACCATGTCCGCGCTGGCGGGTG
  AAGATGTCTGGAGGTGTCCAGGCTGTGGGGACCACATTGCTCCAAGCCAGATATGGTACAGGACTGTCAA
  CGAAACCTGGCACGGCTCTTGCTTCCGGTGTTCAGAATGCCAGGATTCCCTCACCAACTGGTACTATGAG
  AAGATGGGAAGGCTTCTTACTTGCCCCCAAGGACACTACCTGGGGGAAGAGTTTTGGGGATTCTCGTTCA
  TGGTTGCTCCCTGGCTGATGAACAAGGCCCCTTTTAATGGGGCCTGGGGGAGTTCAATTCCCACCCCAAA
  TTGCTTTTGCTTGGTTTAACCCTGCAGGGGGATTCTTTGGGAATGGGGAAAGCCATTTCCCTGGGTCCCC
  CTGTCCCCCCCCTCAATTGGGGGAAATGTGCCAAAGGGGGGGGGGGCCGGGCCCCCATTTTTTGAAAAAA
  CTCCCCAAAAAATTTTTTTCAGGAACCTGTCCCCCTATTTTTGCCCCCTTATTCTTGTCGGGGCCCACCA
  TAAAAGGGGGGGGGGGGTTTTTCCCGTTTTCCGGGGAAAGAGGTGTTTTTAAAATAACGACAATTGGGGC
  AATAAAAAAAAGCACCCCGGGAGTCCCCGATCCCCCAAAATAAAAAACCCCCCCCCCCCTGGGGCACCCT
  CCTCCGGAAATTATGGGGGGCACTTCTTCGCTTTTTTTGTGTGGAGGGTGGGGGAAGACCTTTTCACCCA
  AAGCCCAAATATTCTCTGTTGTGAGAATAATAGCACCTCTCCCAACCCGTAGCAACATGGCATCGTAAGC
  GCGCGGTTCTCTATGTCTGTAATAATGACTACCACTCCACCCTCTTCTCTCGCGTGCACGACGAGAATAT
  
  Kinase domain sequence

  >SC323590 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TKAYTTCTGCTGTGACTATCCATGGGGAGGTCCTGGGGAAGGGCTTCTTTGGGCAGGCTATCAAGGTGAC
  ACACAAAGCCACGGGCAAAGTGATGGTCATGATGGAGTTAATTCGATGTGATGAGGAGACCCAGAAAACT
  TTTCTGACTGAGGTGAAAGTGATGCGCAGCCTGGACCACCCCAATGTGCTCAAGTTCATTGGTGTGCTGT
  ACAAGGATAAGAAGCTGAACCTGCTGACAGAGTACATTGAGGGGG
  
• Restriction Sites: Please inquire
• ACCN: NM_005569
• Insert Size: 4690 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005569.3, NP_005560.1
• RefSeq Size: 3701 bp
• RefSeq ORF: 1917 bp
• Locus ID: 3985
• Cytogenetics: 22q12.2
• Domains: pkinase, TyrKc, PDZ, LIM, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton
• Gene Summary: 'There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2a) differs in the 5' UTR and coding region as well as the 3' UTR and coding region, compared to variant 1. The resulting isoform (2a) is shorter and has distinct N- and C-termini compared to isoform 1.