LIM kinase 2 (LIMK2) (NM_005569) Human Untagged Clone

CAT#: SC323590

LIMK2 (untagged)-Kinase deficient mutant (K360M) of Human LIM domain kinase 2 (LIMK2), transcript variant 2a


  "NM_005569" in other vectors (5)

Reconstitution Protocol

USD 1,080.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol LIMK2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_005569, the custom clone sequence may differ by one or more nucleotides


ATGTCCGCGCTGGCGGGTGAAGATGTCTGGAGGTGTCCAGGCTGTGGGGACCACATTGCTCCAAGCCAGA
TATGGTACAGGACTGTCAACGAAACCTGGCACGGCTCTTGCTTCCGGTGTTCAGAATGCCAGGATTCCCT
CACCAACTGGTACTATGAGAAGGATGGGAAGCTCTACTGCCCCAAGGACTACTGGGGGAAGTTTGGGGAG
TTCTGTCATGGGTGCTCCCTGCTGATGACAGGGCCTTTTATGGTGGCTGGGGAGTTCAAGTACCACCCAG
AGTGCTTTGCCTGTATGAGCTGCAAGGTGATCATTGAGGATGGGGATGCATATGCACTGGTGCAGCATGC
CACCCTCTACTGTGGGAAGTGCCACAATGAGGTGGTGCTGGCACCCATGTTTGAGAGACTCTCCACAGAG
TCTGTTCAGGAGCAGCTGCCCTACTCTGTCACGCTCATCTCCATGCCGGCCACCACTGAAGGCAGGCGGG
GCTTCTCCGTGTCCGTGGAGAGTGCCTGCTCCAACTACGCCACCACTGTGCAAGTGAAAGAGGTCAACCG
GATGCACATCAGTCCCAACAATCGAAACGCCATCCACCCTGGGGACCGCATCCTGGAGATCAATGGGACC
CCCGTCCGCACACTTCGAGTGGAGGAGGTGGAGGATGCAATTAGCCAGACGAGCCAGACACTTCAGCTGT
TGATTGAACATGACCCCGTCTCCCAACGCCTGGACCAGCTGCGGCTGGAGGCCCGGCTCGCTCCTCACAT
GCAGAATGCCGGACACCCCCACGCCCTCAGCACCCTGGACACCAAGGAGAATCTGGAGGGGACACTGAGG
AGACGTTCCCTAAGGCGCAGTAACAGTATCTCCAAGTCCCCTGGCCCCAGCTCCCCAAAGGAGCCCCTGC
TGTTCAGCCGTGACATCAGCCGCTCAGAATCCCTTCGTTGTTCCAGCAGCTATTCACAGCAGATCTTCCG
GCCCTGTGACCTAATCCATGGGGAGGTCCTGGGGAAGGGCTTCTTTGGGCAGGCTATCAAGGTGACACAC
AAAGCCACGGGCAAAGTGATGGTCATGAAAGAGTTAATTCGATGTGATGAGGAGACCCAGAAAACTTTTC
TGACTGAGGTGAAAGTGATGCGCAGCCTGGACCACCCCAATGTGCTCAAGTTCATTGGTGTGCTGTACAA
GGATAAGAAGCTGAACCTCCTGACAGAGTACATTGAGGGGGGCACACTGAAGGACTTTCTGCGCAGTATG
GATCCGTTCCCCTGGCAGCAGAAGGTCAGGTTTGCCAAAGGAATCGCCTCCGGAATGGCCTATTTGCACT
CTATGTGCATCATCCACCGGGATCTGAACTCGCACAACTGCCTCATCAAGTTGGACAAGACTGTGGTGGT
GGCAGACTTTGGGCTGTCACGGCTCATAGTGGAAGAGAGGAAAAGGGCCCCCATGGAGAAGGCCACCACC
AAGAAACGCACCTTGCGCAAGAACGACCGCAAGAAGCGCTACACGGTGGTGGGAAACCCCTACTGGATGG
CCCCTGAGATGCTGAACGGAAAGAGCTATGATGAGACGGTGGATATCTTCTCCTTTGGGATCGTTCTCTG
TGAGATCATTGGGCAGGTGTATGCAGATCCTGACTGCCTTCCCCGAACACTGGACTTTGGCCTCAACGTG
AAGCTTTTCTGGGAGAAGTTTGTTCCCACAGATTGTCCCCCGGCCTTCTTCCCGCTGGCCGCCATCTGCT
GCAGACTGGAGCCTGAGAGCAGACCAGCATTCTCGAAATTGGAGGACTCCTTTGAGGCCCTCTCCCTGTA
CCTGGGGGAGCTGGGCATCCCGCTGCCTGCAGAGCTGGAGGAGTTGGACCACACTGTGAGCATGCAGTAC
GGCCTGACCCGGGACTCACCTCCCTAG


>OriGene 5' read for mutant NM_005569 unedited
ACCGCCCGTATCAGCAATGGGCGGTAGGCGCTGTACGGATGGGAGGTCTATATAAGCAGAGCTCGTTTAG
TGAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAACTAAGGG
GAGCTGCTGTGTCCCCCGCCTCCTCCTCCCCATTTCCGCGCTCCCGGGACCATGTCCGCGCTGGCGGGTG
AAGATGTCTGGAGGTGTCCAGGCTGTGGGGACCACATTGCTCCAAGCCAGATATGGTACAGGACTGTCAA
CGAAACCTGGCACGGCTCTTGCTTCCGGTGTTCAGAATGCCAGGATTCCCTCACCAACTGGTACTATGAG
AAGATGGGAAGGCTTCTTACTTGCCCCCAAGGACACTACCTGGGGGAAGAGTTTTGGGGATTCTCGTTCA
TGGTTGCTCCCTGGCTGATGAACAAGGCCCCTTTTAATGGGGCCTGGGGGAGTTCAATTCCCACCCCAAA
TTGCTTTTGCTTGGTTTAACCCTGCAGGGGGATTCTTTGGGAATGGGGAAAGCCATTTCCCTGGGTCCCC
CTGTCCCCCCCCTCAATTGGGGGAAATGTGCCAAAGGGGGGGGGGGCCGGGCCCCCATTTTTTGAAAAAA
CTCCCCAAAAAATTTTTTTCAGGAACCTGTCCCCCTATTTTTGCCCCCTTATTCTTGTCGGGGCCCACCA
TAAAAGGGGGGGGGGGGTTTTTCCCGTTTTCCGGGGAAAGAGGTGTTTTTAAAATAACGACAATTGGGGC
AATAAAAAAAAGCACCCCGGGAGTCCCCGATCCCCCAAAATAAAAAACCCCCCCCCCCCTGGGGCACCCT
CCTCCGGAAATTATGGGGGGCACTTCTTCGCTTTTTTTGTGTGGAGGGTGGGGGAAGACCTTTTCACCCA
AAGCCCAAATATTCTCTGTTGTGAGAATAATAGCACCTCTCCCAACCCGTAGCAACATGGCATCGTAAGC
GCGCGGTTCTCTATGTCTGTAATAATGACTACCACTCCACCCTCTTCTCTCGCGTGCACGACGAGAATAT
>SC323590 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TKAYTTCTGCTGTGACTATCCATGGGGAGGTCCTGGGGAAGGGCTTCTTTGGGCAGGCTATCAAGGTGAC
ACACAAAGCCACGGGCAAAGTGATGGTCATGATGGAGTTAATTCGATGTGATGAGGAGACCCAGAAAACT
TTTCTGACTGAGGTGAAAGTGATGCGCAGCCTGGACCACCCCAATGTGCTCAAGTTCATTGGTGTGCTGT
ACAAGGATAAGAAGCTGAACCTGCTGACAGAGTACATTGAGGGGG
Restriction Sites Please inquire     
ACCN NM_005569
Insert Size 4690 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005569.3, NP_005560.1
RefSeq Size 3701 bp
RefSeq ORF 1917 bp
Locus ID 3985
Cytogenetics 22q12.2
Domains pkinase, TyrKc, PDZ, LIM, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton
Gene Summary 'There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (2a) differs in the 5' UTR and coding region as well as the 3' UTR and coding region, compared to variant 1. The resulting isoform (2a) is shorter and has distinct N- and C-termini compared to isoform 1.

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