c Maf (MAF) (NM_005360) Human Untagged Clone

CAT#: SC116772

MAF (untagged)-Human v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian) (MAF), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAF
• Synonyms: AYGRP; c-MAF; CCA4; CTRCT21
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005360, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCATCAGAACTGGCAATGAGCAACTCCGACCTGCCCACCAGTCCCCTGGCCATGGAATATGTTAATG
  ACTTCGATCTGATGAAGTTTGAAGTGAAAAAGGAACCGGTGGAGACCGACCGCATCATCAGCCAGTGCGG
  CCGTCTCATCGCCGGGGGCTCGCTGTCCTCCACCCCCATGAGCACGCCGTGCAGCTCGGTGCCCCCTTCC
  CCCAGCTTCTCGGCGCCCAGCCCGGGCTCGGGCAGCGAGCAGAAGGCGCACCTGGAAGACTACTACTGGA
  TGACCGGCTACCCGCAGCAGCTGAACCCCGAGGCGCTGGGCTTCAGCCCCGAGGACGCGGTCGAGGCGCT
  CATCAGCAACAGCCACCAGCTCCAGGGCGGCTTCGATGGCTACGCGCGCGGGGCGCAGCAGCTGGCCGCG
  GCGGCCGGGGCCGGTGCCGGCGCCTCCTTGGGCGGCAGCGGCGAGGAGATGGGCCCCGCCGCCGCCGTGG
  TGTCCGCCGTGATCGCCGCGGCCGCCGCGCAGAGCGGCGCGGGCCCGCACTACCACCACCACCACCACCA
  CGCCGCCGGCCACCACCACCACCCGACGGCCGGCGCGCCCGGCGCCGCGGGCAGCGCGGCCGCCTCGGCC
  GGTGGCGCTGGGGGCGCGGGCGGCGGTGGCCCGGCCAGCGCTGGGGGCGGCGGCGGCGGCGGCGGCGGCG
  GAGGCGGCGGGGGCGCGGCGGGGGCGGGGGGCGCCCTGCACCCGCACCACGCCGCCGGCGGCCTGCACTT
  CGACGACCGCTTCTCCGACGAGCAGCTGGTGACCATGTCTGTGCGCGAGCTGAACCGGCAGCTGCGCGGG
  GTCAGCAAGGAGGAGGTGATCCGGCTGAAGCAGAAGAGGCGGACCCTGAAAAACCGCGGCTATGCCCAGT
  CCTGCCGCTTCAAGAGGGTGCAGCAGAGACACGTCCTGGAGTCGGAGAAGAACCAGCTGCTGCAGCAAGT
  CGACCACCTCAAGCAGGAGATCTCCAGGCTGGTGCGCGAGAGGGACGCGTACAAGGAGAAATACGAGAAG
  TTGGTGAGCAGCGGCTTCCGAGAAAACGGCTCGAGCAGCGACAACCCGTCCTCTCCCGAGTTTTTCATAA
  CTGAGCCCACTCGCAAGTTGGAGCCATCAGTGGGATACGCCACATTTTGGAAGCCCCAGCATCGTGTACT
  TACCAGTGTGTTCACAAAATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005360 unedited
  TATTTTGTAATACGACTCACTATAGGGCGGCCGCGCGGCAAGCTGGCTCACCCGCTGGCC
  ACCCAGCACAGCCCGCTGGCCCCTCTCCTGCAGCCCATCTGGCGGAGCGGCGGCGGCGGC
  GGCGGCGGCAGGAGAATGGCATCAGAACTGGCAATGAGCAACTCCGACCTGCCCACCAGT
  CCCCTGGCCATGGAATATGTTAATGACTTCGATCTGATGAAGTTTGAAGTGAAAAAGGAA
  CCGGTGGAGACCGACCGCATCATCAGCCAGTGCGGCCGTCTCATCGCCGGGGGCTCGCTG
  TCCTCCACCCCCATGAGCACGCCGTGCAGATCGGTGCCCCCTTCCCCCAGCTTCTCGGCG
  CCCAGCCCGGGCTCGGGCAGCGAGCAGAAGGCGCACCTGGAAGACTACTACTGGATGACC
  GGCTACCCGCAGCAGCTGAACCCCGAGGCGCTGGGCTTCAGCCCCGAGGACGCGGTCGAG
  GCGCTCATCAGCAACAGCCACCAGCTTCAGGGCGGCTCCGATGGCTACGCGCGCGGCGCC
  GCATATCTGGCCGCGGCGGCCGGGGCCGGCGCCGGCGCCTCCTTGGGCGGCAGTGGTCGA
  CGAGATGGGCCCCGCCGCCGGCGTGGCGTTCGCCGCGATCCCCCGTGGCCGCCGCTCAAA
  CCGGTGTGTGCCCCGCCTATCCCGACCGATTCCACCACGCCGATCGGCACCACCACCCCC
  CCACTGTTCCCCCCGTCCGGCTCTCTCCGAATACCCCGCCCCACACCTGACAGGCGGTCC
  CCGGGGCCCCTTTCTTCTTTGATCCCGCTCCCTTCTGCGGCCCCCGCCGTTGCCCCCCCC
  ACATAACGCTCTTCCGTCCCCTCGCCTCTCCACCCCCTATCCACCCACCCCCCTTTTTCC
  TACTTCCCCCCCCCCTCCTCTCTCCCCCCTCCCCTTTCCGCCCCCTGCCCTTCCTTCCCA
  CTCCGCACCCCCCCCCCGTCCTCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005360 unedited
  TTGTTTACTAGCGCGCATGCNGNAGCNGAGTAANNCTTTTCCTTTTTTCTCTAACACATT
  TATTTTTATTTAAAAAGGAAAATAACCAGTAATCAGGGGTAGGTGGTTCTATCATGACTG
  CAAATAATAATAATAATGATGATTTTTTTTAATGTACAGTTTTCACACAAATTTCATTTT
  GTGAACACACTGGTAAGTACACGATGTTGGGTCACTCTAAAGGGGGCGAATCCCATTGAT
  GGCTCCAACTTGCGAGTGGGCTCAGTTATGAAAAACTCGGGAGAGGACGGGTTGTCGCTG
  CTCGAGCCGTTTTCTCGGAAGCCGCTGCTCACCAACTTCTCGTATTTCTCCTTGTACGCG
  TCCCTCTCGCGCACCAGCCTGGAGATCTCCTGCTTGAGGTGGTCGACTTGCTGCAGCAGC
  TGGTTCTTCTCCGACTCCAGGACGTGTCTCTGCTGCACCCTCTTGAAGCGGCAGGACTGG
  GCATAGCCGCGGTTTTTCAGGGTCCCGCTCTTCTGCTTCAGACGGATCACCTCCTCCTTG
  TGGACCCGCGCACCGGCCGTTCATCTGCGCAAGAGCTGGCACCAACTGGTCTCGGAAAAA
  CGGTCGCCCAATGCAGGCCGACGGGGCCCTGTCCGGGATCCGGCTCCCCCCCGCCCGCGG
  GGGCCCCGTCGGCTTCGGGGGGCGCGGCGTCGCGGGTCCCCCCCCGGTTGGCGGGGCACC
  CGTTAGCCGGCCCCAGGGACATTCGCTAAAGTGGGCCCGCGGGCCGCCCGGGCCCGGGCC
  CCCGCTCGTCCGCCCGGCCTTCCGCCCCCCGCCCCTGGGCGCGGATGGGTATATTGCGAT
  GACGAACCACGTTACGCGGACTGACGAGGATAAGGAGGCTCCGCTCTCGTGACGGTCGGC
  CGATCACGTCCCGGGTGGCGCCGGTGGCGGGGTTACGAACGCGCCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005360
• Insert Size: 1480 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005360.3, NP_005351.2
• RefSeq Size: 2161 bp
• RefSeq ORF: 1212 bp
• Locus ID: 4094
• Cytogenetics: 16q23.2
• Domains: bZIP_Maf, BRLZ
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'The protein encoded by this gene is a DNA-binding, leucine zipper-containing transcription factor that acts as a homodimer or as a heterodimer. Depending on the binding site and binding partner, the encoded protein can be a transcriptional activator or repressor. This protein plays a role in the regulation of several cellular processes, including embryonic lens fiber cell development, increased T-cell susceptibility to apoptosis, and chondrocyte terminal differentiation. Defects in this gene are a cause of juvenile-onset pulverulent cataract as well as congenital cerulean cataract 4 (CCA4). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]'
  Transcript Variant: This variant (1) is spliced and encodes the longer isoform (a).