H3.3B (H3F3B) (NM_005324) Human Untagged Clone

CAT#: SC116824

H3F3B (untagged)-Human H3 histone, family 3B (H3.3B) (H3F3B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: H3F3B
• Synonyms: H3.3B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116824 sequence for NM_005324 edited (data generated by NextGen Sequencing)
  ATGGCCCGAACCAAGCAGACTGCTCGTAAGTCCACCGGTGGGAAAGCCCCCCGCAAACAG
  CTGGCCACGAAAGCCGCCAGGAAAAGCGCTCCCTCTACCGGCGGGGTGAAGAAGCCTCAT
  CGCTACAGGCCCGGGACCGTGGCGCTTCGAGAGATTCGTCGTTATCAGAAGTCGACCGAG
  CTGCTCATCCGGAAGCTGCCCTTCCAGAGGTTGGTGAGGGAGATCGCGCAGGATTTCAAA
  ACCGACCTGAGGTTTCAGAGCGCAGCCATCGGTGCGCTGCAGGAGGCTAGCGAAGCGTAC
  CTGGTGGGTCTGTTCGAAGATACCAACCTGTGTGCCATCCACGCTAAGAGAGTCACCATC
  ATGCCCAAAGACATCCAGTTGGCTCGCCGGATACGGGGAGAGAGAGCTTAA
  
  Clone variation with respect to NM_005324.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005324 unedited
  NGTGTGCACCATTATGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGT
  TTTTCGCTCGTCGACTGCGGCTCTTCCTCGGGCAGCGGAAGCGGCGCGGCGGTCGGAGAA
  GTGGCCTAAAACTTCGGCGTTGGGTGAAAGAAAATGGCCCGAACCAAGCAGACTGCTCGT
  AAGTCCACCGGTGGGAAAGCCCCCATGCAAACAGCTGGCCACGAAAGCCGCCAGGAAAAG
  CGCTCCCTCTACCGGCGGGGTGAAGAAGCCTCATCGCTACAGGCCCGGGACCGTGGCGCT
  TCGAGAGATTCGTCGTTATCAGAAGTCGATCCGAGCTGCTCATCCGGAAGCTGCCCTTCC
  AGAGGTTGGTGAGGGAGATCGCGCAGGATTTCAAAACCGACCTGAGGTTTCAGAGCGCAG
  CCATCGGTGCGCTGCAGGAGGCTAGCGAAGCGTACCTATGTGGGTCTGTTCGAAGATACC
  AACCTGTGTGCCATCCACGCTAAGAGAGTCACCATCATGCCCAAATACATCCAGTTGGCT
  CGCCGGATACGGNGAGAGAGAGCTTAAGTGAAGGCAGTTTTTATGGCGTTTTGTAGTAAA
  TTCTGTAAAATACTTTGGTTTAATTTGTGACTNTTTTTGTAAGAAATTGTTTATAATATG
  TTGCATTTGTACTTAAGTCATTCCATCTTTCACTCANGATGAATGCGAAAAGTGACTGTT
  CACAGACCTCAGTGATGTGAGCACTGTTGCTCANGAGTGACAAGTTGCTAATATGCAGAA
  GGGATGGGTGATACTTCTTGCTTCTCATGATGCATGTTTCTGTATGTTAATGACTTGTTG
  GGTAGCTATTAAGTACTAGAGTTGATTAATGTGTACAGGGTCCTTTTGCATANAACATTT
  ATGATCTGATCCAGTGTTACTACAATGGGCTGTTAATCTGACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005324 unedited
  CACCACCCCCAAAACATCCTTCCTTTTCCCTTTTATTTGCCCGCGGCCGCATTCTANGAT
  CGGTTTTTTTTTTTTTTTTTTGGGAAAACAAGTTTATTTGTAAATTTAGTCAACATACAT
  AATTGACCTAAAAACTTCAGTAAATTTTAAAACCACTTGCGAAGGCCATCTCTATAAAAA
  TGATTTTCCCAGGACAGTAACCAGATGTAACCTAACCCAACACCATCTTAATTGGCAGAG
  GTTCAGTGGGCTGGAGTTTTGTGCTCCTCCCCCACACCAAGTTTTTATAATACAAATGCC
  ACAAGAAAAAGAACTTCGGTACTGTTTCCTCAGCAGAGGAGAAAAACTCAACCTAGTTAT
  GAGACCAACCACACAACACAATGAAAAGCTGCACTAACTAGTTCAGAATGTTAGTTAAGA
  TGATGCTGGTGTGAATAACTCGCTTTTTCTAGAGCCCTTATAAATAAAATCCCCCAGTTA
  GTGTTTGCATTATCAGCTACAGGGTTAACATGTGGTACAATGAGGACTTATGCAAGGCAT
  AAATGCGCATAGCATTTTACTACTATGAGAACAAGTGCAGTCAGAAGAAAACCACTGGAC
  TCTAAATTACACCCACCTTAATGACCAGACTCCCCACCACTTGTGAATGTACAACACTCC
  ACTTTAAAAACGTCGATCCTACCATATATAAAATAGCTATTATAACTGCACATACTGCAT
  TCTATTACCGCCAGGCTTACTTTTTTTTTTTTTTAAAGGAAAACTGTAAGTCACCCTGCG
  GGTAAAACCTCGTACTTTCACCCCTTGAAAAAGCCCAACTTTTTTTCACAGGGATGTATG
  GCCCACATTTACCGCCCCCATTGGTAAACCCTGGGATTCAATCATAACACCTTTTTTTTG
  GCAAAGGACCCTGGAAAATTTTACAACCCTTAGACCCTTCATAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005324
• Insert Size: 1730 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005324.3, NP_005315.1
• RefSeq Size: 2753 bp
• RefSeq ORF: 411 bp
• Locus ID: 3021
• Cytogenetics: 17q25.1
• Domains: H3, histone
• Protein Pathways: Systemic lupus erythematosus
• Gene Summary: 'Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded by this gene is a replication-independent histone that is a member of the histone H3 family. Pseudogenes of this gene have been identified on the X chromosome, and on chromosomes 5, 13 and 17. [provided by RefSeq, Oct 2015]'