GATA6 (NM_005257) Human Untagged Clone

CAT#: SC116877

GATA6 (untagged)-Human GATA binding protein 6 (GATA6)


  "NM_005257" in other vectors (6)

Reconstitution Protocol

USD 1,010.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol GATA6
Synonyms GATA-binding protein 6; GATA binding protein 6; transcription factor GATA-6
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116877 sequence for NM_005257 edited (data generated by NextGen Sequencing)
ATGGCCTTGACTGACGGCGGCTGGTGCTTGCCGAAGCGCTTCGGGGCCGCGGGTGCGGAC
GCCAGCGACTCCAGAGCCTTTCCAGCGCGGGAGCCCTCCACGCCGCCTTCCCCCATCTCT
TCCTCGTCCTCCTCCTGCTCCCGGGGCGGAGAGCGGGGCCCCGGCGGCGCCAGCAACTGC
GGGACGCCTCAGCTCGACACGGAGGCGGCGGCCGGACCCCCGGCCCGCTCGCTGCTGCTC
AGTTCCTACGCTTCGCATCCCTTCGGGGCTCCCCACGGACCTTCGGCGCCTGGGGTCGCG
GGCCCCGGGGGCAACCTGTCGAGCTGGGAGGACTTGCTGCTGTTCACTGACCTCGACCAA
GCCGCGACCGCCAGCAAGCTGCTGTGGTCCAGCCGCGGCGCCAAGCTGAGCCCCTTCGCA
CCCGAGCAGCCGGAGGAGATGTACCAGACCCTCGCCGCTCTCTCCAGCCAGGGTCCGGCC
GCCTACGACGGCGCGCCCGGCGGCTTCGTGCACTCTGCGGCCGCGGCGGCAGCAGCCGCG
GCGGCGGCCAGCTCCCCGGTCTACGTGCCCACCACCCGCGTGGGTTCCATGCTGCCCGGC
CTACCGTACCACCTGCAGGGGTCGGGCAGTGGGCCAGCCAACCACGCGGGCGGCGCGGGC
GCGCACCCCGGCTGGCCTCAGGCCTCGGCCGACAGCCCTCCATACGGCAGCGGAGGCGGC
GCGGCTGGCGGCGGGGCCGCGGGGCCTGGCGGCGCTGGCTCAGCCGCGGCGCACGTCTCG
GCGCGCTTCCCCTACTCTCCCAGCCCGCCCATGGCCAACGGCGCCGCGCGGGAGCCGGGA
GGCTACGCGGCGGCGGGCAGTGGGGGCGCGGGAGGCGTGAGCGGCGGCGGCAGTAGCCTG
GCGGCCATGGGCGGCCGCGAGCCCCAGTACAGCTCGCTGTCGGCCGCGCGGCCGCTGAAC
GGGACGTACCACCACCACCACCACCACCACCACCACCATCCGAGCCCCTACTCGCCCTAC
GTGGGGGCGCCACTGACGCCTGCCTGGCCCGCCGGACCCTTCGAGACCCCGGTGCTGCAC
AGCCTGCAGAGCCGCGCCGGAGCCCCGCTCCCGGTGCCCCGGGGTCCCAGTGCAGACCTG
CTGGAGGACCTGTCCGAGAGCCGCGAGTGCGTGAACTGCGGCTCCATCCAGACGCCGCTG
TGGCGGCGGGACGGCACCGGCCACTACCTGTGCAACGCCTGCGGGCTCTACAGCAAGATG
AACGGCCTCAGCCGGCCCCTCATCAAGCCGCAGAAGCGCGTGCCTTCATCACGGCGGCTT
GGATTGTCCTGTGCCAACTGTCACACCACAACTACCACCTTATGGCGCAGAAACGCCGAG
GGTGAACCCGTGTGCAATGCTTGTGGACTCTACATGAAACTCCATGGGGTGCCCAGACCA
CTTGCTATGAAAAAAGAGGGAATTCAAACCAGGAAACGAAAACCTAAGAACATAAATAAA
TCAAAGACTTGCTCTGGTAATAGCAATAATTCCATTCCCATGACTCCAACTTCCACCTCT
TCTAACTCAGATGATTGCAGCAAAAATACTTCCCCCACAACACAACCTACAGCCTCAGGG
GCGGGTGCCCCGGTGATGACTGGTGCGGGAGAGAGCACCAATCCCGAGAACAGCGAGCTC
AAGTATTCGGGTCAAGATGGGCTCTACATAGGCGTCAGTCTCGCCTCGCCGGCCGAAGTC
ACGTCCTCCGTGCGACCGGATTCCTGGTGCGCCCTGGCCCTGGCCTGA

Clone variation with respect to NM_005257.3
>OriGene 5' read for NM_005257 unedited
GGTCCTTACACCCGCCCCGTTGACTCAAAGGGCGGTAGGCGTGTACGGTGGNGAGTCTAT
ATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCG
GCCGCGAATTCGGCACGAGGCTTCTCTCCGCGCCTGGGAGCACCCTCCGCCGCGGCCGTT
CTCCATGCGCAGCGCCCGCCCGAGGAGCTAGACGTCAGCTTGGAGCGGCGCCGGACCGTG
GATGGCCTTGACTGACGGCGGCTGGTGCTTGCCGAAGCGCTTCGGGGCCGCGGGTGCGGA
CGCCAGCGACTCCAGAGCCTTTCCAGCGCGGGAGCCCTCCACGCCGCCTTCCCCCATCTC
TTCCTCGTCCTCCTCCTGCTCCCGGGGCGGAGAGCGGGGCCCCGGCGGCGCCAGCAACTG
CGGGACGCCTCAGCTCGACACGGAGGCGGCGGCCGGACCCCCGGCCCGCTCGCTGCTGCT
CAGTTCCTACGCTTCGCATCCCTTCGGGGCTCCCCACGGACCTTCGGCGCCTGGGGTCGC
GGGCCCCGGGGGCAACCTGTCGAGCTGGGAGGACTTGCTGCTGTTCACTGACCTCGACCA
AGCCGCGACCGCCAGCAAGCTGCTGTGGTCCAGCCGCGGCGCCAAGCTGAGCCCCTTCGC
ACCCGAGCAGCCGGAGGAGATGTACCAGACCCTCGCCGCTCTCTCCAGCCAGGGTCCGGC
CGCCTACGACGGCGCGCCCGGCGGCTTCGTGCACTCTGCGGCCGCGGCGGCAGCAGCCGC
GGCGGCGGCCAGCTCCCCAGTCTACGTGCCCACCACCCGCGTGGGTTCCATGCTGCCCAG
CCTACCGTACCCCTGCAGGGGTCGGGCAGTGGGCCAGCCAACACGCGGGCGGGCGCGGCG
CGCACCCAGCTGGNCTCATGCCTCAGCCGACAGCCTC
>OriGene 3' read for NM_005257 unedited
TAGCTNTGNNACCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTAC
AGAAAACAAAACAATACCCTTTTTATTTTTAAAACTCTGGTGAAATAGGTTTCCATAGGA
CATCCCATTCAAAAAAAAAAATTAATGTAAACCAACCTGCCTGTGGGTTAGTCACACTAT
ACAGACTTCATCAGAGTGTTTACAGATACCTAGAGTATTTACAAATGCTATCCGTTAGGT
AGTAAAGTGTTGTTGCAATTTTTCCAGCACATATTCCACAGTAAAATAAAGGGGGTATAT
TGGTGTTCTTGTATAAAAATTCTTAACAAATAGAGAAAGGCAATTTTCCTGAGAGAAGAT
TTGTTCTTGAAATATTTTTCTTTAACAATTGGAAATATAAAATGGGATGTAGTTGTAAAA
GAAGCATAAGAATGTCTAAGTTAACATTTTTCTTTTAAATTATTTTAAGTACCATTAAGT
TAAAATGTTTACATTCATTATAAAAAATGCTGTTAAATCTTATGCAAATGATTTAACACT
GATTGCTGCAACGCAAATTGCACTTTTTAAAAAAATTATTGTATACATGGAAGAAAAATA
TCTTGTTTGCTACCCTGTTTACAAATTCCGACTGACTTCAGATCAGCCACACAATATGAA
CTTTGAGAAACGTCAGTCACAGTGTATGTTAAAGTTACAATGCAGTCTTGCAAACTGAGT
AATATTTTTGATAAGAGACCTCATGAACCGACTCAGCCTCCAGAGATGTGTACCAAATGG
CCTCAAGTGATCTTCTCCATGAACGCACATGAAATCTGGATGTGGNAAAGGTATGAAGAA
ATTCTGGNAACACCAAATTGATCAGAAGAAGCCATGATTTTGGACAAATGCAAAATCTTT
TTAAAAACTCTCTCATATTCATAGCAGCAGGGCCAGTCTAGTCTGCTTCTGAAGTGGACA
GGTCCGCCC
Restriction Sites NotI-NotI     
ACCN NM_005257
Insert Size 3100 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005257.3, NP_005248.2
RefSeq Size 3494 bp
RefSeq ORF 1788 bp
Locus ID 2627
Cytogenetics 18q11.2
Domains GATA, GATA-N
Protein Families Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors
Gene Summary 'This gene is a member of a small family of zinc finger transcription factors that play an important role in the regulation of cellular differentiation and organogenesis during vertebrate development. This gene is expressed during early embryogenesis and localizes to endo- and mesodermally derived cells during later embryogenesis and thereby plays an important role in gut, lung, and heart development. Mutations in this gene are associated with several congenital defects. [provided by RefSeq, Mar 2012]'

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.