GATA6 (NM_005257) Human Untagged Clone

CAT#: SC116877

GATA6 (untagged)-Human GATA binding protein 6 (GATA6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GATA6
• Synonyms: GATA-binding protein 6; GATA binding protein 6; transcription factor GATA-6
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116877 sequence for NM_005257 edited (data generated by NextGen Sequencing)
  ATGGCCTTGACTGACGGCGGCTGGTGCTTGCCGAAGCGCTTCGGGGCCGCGGGTGCGGAC
  GCCAGCGACTCCAGAGCCTTTCCAGCGCGGGAGCCCTCCACGCCGCCTTCCCCCATCTCT
  TCCTCGTCCTCCTCCTGCTCCCGGGGCGGAGAGCGGGGCCCCGGCGGCGCCAGCAACTGC
  GGGACGCCTCAGCTCGACACGGAGGCGGCGGCCGGACCCCCGGCCCGCTCGCTGCTGCTC
  AGTTCCTACGCTTCGCATCCCTTCGGGGCTCCCCACGGACCTTCGGCGCCTGGGGTCGCG
  GGCCCCGGGGGCAACCTGTCGAGCTGGGAGGACTTGCTGCTGTTCACTGACCTCGACCAA
  GCCGCGACCGCCAGCAAGCTGCTGTGGTCCAGCCGCGGCGCCAAGCTGAGCCCCTTCGCA
  CCCGAGCAGCCGGAGGAGATGTACCAGACCCTCGCCGCTCTCTCCAGCCAGGGTCCGGCC
  GCCTACGACGGCGCGCCCGGCGGCTTCGTGCACTCTGCGGCCGCGGCGGCAGCAGCCGCG
  GCGGCGGCCAGCTCCCCGGTCTACGTGCCCACCACCCGCGTGGGTTCCATGCTGCCCGGC
  CTACCGTACCACCTGCAGGGGTCGGGCAGTGGGCCAGCCAACCACGCGGGCGGCGCGGGC
  GCGCACCCCGGCTGGCCTCAGGCCTCGGCCGACAGCCCTCCATACGGCAGCGGAGGCGGC
  GCGGCTGGCGGCGGGGCCGCGGGGCCTGGCGGCGCTGGCTCAGCCGCGGCGCACGTCTCG
  GCGCGCTTCCCCTACTCTCCCAGCCCGCCCATGGCCAACGGCGCCGCGCGGGAGCCGGGA
  GGCTACGCGGCGGCGGGCAGTGGGGGCGCGGGAGGCGTGAGCGGCGGCGGCAGTAGCCTG
  GCGGCCATGGGCGGCCGCGAGCCCCAGTACAGCTCGCTGTCGGCCGCGCGGCCGCTGAAC
  GGGACGTACCACCACCACCACCACCACCACCACCACCATCCGAGCCCCTACTCGCCCTAC
  GTGGGGGCGCCACTGACGCCTGCCTGGCCCGCCGGACCCTTCGAGACCCCGGTGCTGCAC
  AGCCTGCAGAGCCGCGCCGGAGCCCCGCTCCCGGTGCCCCGGGGTCCCAGTGCAGACCTG
  CTGGAGGACCTGTCCGAGAGCCGCGAGTGCGTGAACTGCGGCTCCATCCAGACGCCGCTG
  TGGCGGCGGGACGGCACCGGCCACTACCTGTGCAACGCCTGCGGGCTCTACAGCAAGATG
  AACGGCCTCAGCCGGCCCCTCATCAAGCCGCAGAAGCGCGTGCCTTCATCACGGCGGCTT
  GGATTGTCCTGTGCCAACTGTCACACCACAACTACCACCTTATGGCGCAGAAACGCCGAG
  GGTGAACCCGTGTGCAATGCTTGTGGACTCTACATGAAACTCCATGGGGTGCCCAGACCA
  CTTGCTATGAAAAAAGAGGGAATTCAAACCAGGAAACGAAAACCTAAGAACATAAATAAA
  TCAAAGACTTGCTCTGGTAATAGCAATAATTCCATTCCCATGACTCCAACTTCCACCTCT
  TCTAACTCAGATGATTGCAGCAAAAATACTTCCCCCACAACACAACCTACAGCCTCAGGG
  GCGGGTGCCCCGGTGATGACTGGTGCGGGAGAGAGCACCAATCCCGAGAACAGCGAGCTC
  AAGTATTCGGGTCAAGATGGGCTCTACATAGGCGTCAGTCTCGCCTCGCCGGCCGAAGTC
  ACGTCCTCCGTGCGACCGGATTCCTGGTGCGCCCTGGCCCTGGCCTGA
  
  Clone variation with respect to NM_005257.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005257 unedited
  GGTCCTTACACCCGCCCCGTTGACTCAAAGGGCGGTAGGCGTGTACGGTGGNGAGTCTAT
  ATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCG
  GCCGCGAATTCGGCACGAGGCTTCTCTCCGCGCCTGGGAGCACCCTCCGCCGCGGCCGTT
  CTCCATGCGCAGCGCCCGCCCGAGGAGCTAGACGTCAGCTTGGAGCGGCGCCGGACCGTG
  GATGGCCTTGACTGACGGCGGCTGGTGCTTGCCGAAGCGCTTCGGGGCCGCGGGTGCGGA
  CGCCAGCGACTCCAGAGCCTTTCCAGCGCGGGAGCCCTCCACGCCGCCTTCCCCCATCTC
  TTCCTCGTCCTCCTCCTGCTCCCGGGGCGGAGAGCGGGGCCCCGGCGGCGCCAGCAACTG
  CGGGACGCCTCAGCTCGACACGGAGGCGGCGGCCGGACCCCCGGCCCGCTCGCTGCTGCT
  CAGTTCCTACGCTTCGCATCCCTTCGGGGCTCCCCACGGACCTTCGGCGCCTGGGGTCGC
  GGGCCCCGGGGGCAACCTGTCGAGCTGGGAGGACTTGCTGCTGTTCACTGACCTCGACCA
  AGCCGCGACCGCCAGCAAGCTGCTGTGGTCCAGCCGCGGCGCCAAGCTGAGCCCCTTCGC
  ACCCGAGCAGCCGGAGGAGATGTACCAGACCCTCGCCGCTCTCTCCAGCCAGGGTCCGGC
  CGCCTACGACGGCGCGCCCGGCGGCTTCGTGCACTCTGCGGCCGCGGCGGCAGCAGCCGC
  GGCGGCGGCCAGCTCCCCAGTCTACGTGCCCACCACCCGCGTGGGTTCCATGCTGCCCAG
  CCTACCGTACCCCTGCAGGGGTCGGGCAGTGGGCCAGCCAACACGCGGGCGGGCGCGGCG
  CGCACCCAGCTGGNCTCATGCCTCAGCCGACAGCCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005257 unedited
  TAGCTNTGNNACCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTAC
  AGAAAACAAAACAATACCCTTTTTATTTTTAAAACTCTGGTGAAATAGGTTTCCATAGGA
  CATCCCATTCAAAAAAAAAAATTAATGTAAACCAACCTGCCTGTGGGTTAGTCACACTAT
  ACAGACTTCATCAGAGTGTTTACAGATACCTAGAGTATTTACAAATGCTATCCGTTAGGT
  AGTAAAGTGTTGTTGCAATTTTTCCAGCACATATTCCACAGTAAAATAAAGGGGGTATAT
  TGGTGTTCTTGTATAAAAATTCTTAACAAATAGAGAAAGGCAATTTTCCTGAGAGAAGAT
  TTGTTCTTGAAATATTTTTCTTTAACAATTGGAAATATAAAATGGGATGTAGTTGTAAAA
  GAAGCATAAGAATGTCTAAGTTAACATTTTTCTTTTAAATTATTTTAAGTACCATTAAGT
  TAAAATGTTTACATTCATTATAAAAAATGCTGTTAAATCTTATGCAAATGATTTAACACT
  GATTGCTGCAACGCAAATTGCACTTTTTAAAAAAATTATTGTATACATGGAAGAAAAATA
  TCTTGTTTGCTACCCTGTTTACAAATTCCGACTGACTTCAGATCAGCCACACAATATGAA
  CTTTGAGAAACGTCAGTCACAGTGTATGTTAAAGTTACAATGCAGTCTTGCAAACTGAGT
  AATATTTTTGATAAGAGACCTCATGAACCGACTCAGCCTCCAGAGATGTGTACCAAATGG
  CCTCAAGTGATCTTCTCCATGAACGCACATGAAATCTGGATGTGGNAAAGGTATGAAGAA
  ATTCTGGNAACACCAAATTGATCAGAAGAAGCCATGATTTTGGACAAATGCAAAATCTTT
  TTAAAAACTCTCTCATATTCATAGCAGCAGGGCCAGTCTAGTCTGCTTCTGAAGTGGACA
  GGTCCGCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005257
• Insert Size: 3100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005257.3, NP_005248.2
• RefSeq Size: 3494 bp
• RefSeq ORF: 1788 bp
• Locus ID: 2627
• Cytogenetics: 18q11.2
• Domains: GATA, GATA-N
• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors
• Gene Summary: 'This gene is a member of a small family of zinc finger transcription factors that play an important role in the regulation of cellular differentiation and organogenesis during vertebrate development. This gene is expressed during early embryogenesis and localizes to endo- and mesodermally derived cells during later embryogenesis and thereby plays an important role in gut, lung, and heart development. Mutations in this gene are associated with several congenital defects. [provided by RefSeq, Mar 2012]'