GCHFR (NM_005258) Human Untagged Clone

CAT#: SC116878

GCHFR (untagged)-Human GTP cyclohydrolase I feedback regulator (GCHFR)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GCHFR
• Synonyms: GFRP; HsT16933; P35
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005258 edited
  ATGCCCTACCTGCTCATCAGCACCCAGATCCGCATGGAGGTGGGCCCCACTATGGTGGGC
  GATGAACAGTCGGATCCAGAGCTGATGCAGCATCTGGGGGCTTCAAAGAGAAGAGCCTTG
  GGAAACAACTTTTATGAATACTACGTCGATGACCCTCCCCGCATAGTCCTGGACAAGCTG
  GAACGCAGGGGCTTCCGTGTGCTGAGCATGACGGGGGTGGGCCAGACGCTGGTGTGGTGT
  CTGCACAAGGAGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005258 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATT
  CGGCACGAGGGGGACTCCCAGCTGCGCGTCGCAGTCCCGACGCGAGAAGGGCTGGAGTCG
  GCGTCCAGCCTAGAGCCCCCGGTGGGAGCCAGGCCGGGACGCGTGCACCATGCCCTACCT
  GCTCATCAGCACCCAGATCCGCATGGAGGTGGGCCCCACTATGGTGGGCGATGAACAGTC
  GGATCCAGAGCTGATGCAGCATCTGGGGGCTTCAAAGAGAAGAGCCTTGGGAAACAACTT
  TTATGAATACTACGTCGATGACCCTCCCCGCATAGTCCTGGACAAGCTGGAACGCAGGGG
  CTTCCGTGTGCTGAGCATGACGGGGGTGGGCCAGACGCTGGTGTGGTGTCTGCACAAGGA
  GTGACCTTCTCATGCTGATTTGCAGACGGGGCACCCCTGTGGAGGGGCTGCTGTGGGCCC
  TGACCTCCAAGCTCCTGCCTCACCGTCTGCCTTGCTCCTCTCTTCCCAAATCATCACCGC
  CATGGGCCCAGCCCCAAAGGGCAGTGAATGGCCTTCTCTGAAACCCTGCGTCAAGCAGTG
  GGAGAGGGCAGTGCCCGGTGCCCTGGTGCTCCCAGCTGCCCTCCTGCTTCGGGCCTGGGC
  CGNAGGGCCTGTGTANGCCATGTTCCTNCGGCAGCTGCCCCGGGCCNNGAGCTGGCACTN
  CAGCGGCCCCTGGCGCGTGGCTCCTGCATAGCTAGCCCAAGCAATAAAGGGCTGTGATGA
  GTGGCTGCGCCTGTGCTCTGCTTGTGCACCGGACTCTGGACTCTCACCCTGCGGAGCGTC
  CCTGGGGGGCTGGCCCTTNCGTGGAGGGCGCCCAGCCTGGNTGGACGCTCTGCGCAAACC
  CAGCCCTATACTATGNTGGGGGTAAGGAGGAAAATCTAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005258 unedited
  TGACCCCTTACCCTACATAGGTATAAGGTGCTGGGCTCTGCCGCAGAGCGCTCCACCCAG
  GCCTGGCGCCCCTCCCAGGAGTGGGCAGACCCCCAGGGAAACGCTCCGCAGGGTGAGAGT
  CCCAGAGTCCGTGCACAAGCAGAGCACAGGCGCAGCCACTCATCACAGCCCTTTATTGGC
  TTGGGCTAGCTATGCAGGAGCCACGCGCCAGGGCCGCTGGAGTGCCCAGCTCCGGCCCGG
  GGCAGCTGCCCGAGGAACATGGCCTACACAAGGCCCTCGGCCCAGGCCCGAAGCAGGAGG
  GCAGCTGGGAGCACCAGGGCACCGGGCACTGCCCTCTCCCACTGCTTGACGCAGGGTTTC
  AGAGAAGGCCATTCACTGCCCTTTGGGGCTGGGCCCATGGCGGTGATGATTTGGGAAGAG
  AGGAGCAAGGCAGACGGTGAGGCAGGAGCTTGGAGGTCAGGGCCCACAGCAGCCCCTCCA
  CAGGGGTGCCCCGTCTGCAAATCAGCATGAGAAGGTCACTCCTTGTGCAGACACCACACC
  AGCGTCTGGCCCACCCCCGTCATGCTCAGCACACGGAAGCCCCTGCGTTCCAGCTTGTCC
  AGGACTATGCGGGGAGGGTCATCGACGTAGTATTCATAAAAGTTGTTTCCCAAGGCTCTT
  CTCTTTGAAGCCCCCAGATGCTGCATCAGCTCTGGATCCGACTGTTCATCGCCCACCATA
  GTGGGGCCCACCTNCATGCGGATCTGGGTGCTGATGAGCAGGTAGGGCATGGTGCACCGC
  GTCCCGGCCTGGCTCCCACCGGGGGCTCTAGCTGGACGCCGACTCCAACCCTTNTCGCGT
  TGGGACTGCGAACCGCAACTGGGAGTTCCCCTCGTGCCGAATTCCGGACGAAGCCCTCTT
  GCCGAATTCCCGGCCGNCCTTATATGAGTGGTATAACAAATCTGGACGTTACTAAAGAGC
  CTGCTTATTAGACTTCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005258
• Insert Size: 1090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005258.2, NP_005249.1
• RefSeq Size: 713 bp
• RefSeq ORF: 255 bp
• Locus ID: 2644
• Cytogenetics: 15q15.1
• Gene Summary: 'GTP cyclohydrolase I feedback regulatory protein binds to and mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase I. The regulatory protein, GCHFR, consists of a homodimer. It is postulated that GCHFR may play a role in regulating phenylalanine metabolism in the liver and in the production of biogenic amine neurotransmitters and nitric oxide. [provided by RefSeq, Jul 2008]'