SIAH2 (NM_005067) Human Untagged Clone

CAT#: SC116970

SIAH2 (untagged)-Human seven in absentia homolog 2 (Drosophila) (SIAH2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SIAH2
• Synonyms: hSiah2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116970 sequence for NM_005067 edited (data generated by NextGen Sequencing)
  ATGAGCCGCCCGTCCTCCACCGGCCCCAGCGCTAATAAACCCTGCAGCAAGCAGCCGCCG
  CCGCAGCCCCAGCACACTCCGTCCCCGGCTGCGCCCCCGGCCGCCGCCACCATCTCGGCT
  GCGGGCCCCGGCTCGTCCGCGGTGCCCGCCGCGGCGGCGGTGATCTCGGGCCCCGGCGGC
  GGCGGCGGGGCCGGCCCGGTGTCCCCGCAGCACCACGAGCTGACCTCGCTCTTCGAGTGT
  CCGGTCTGCTTTGACTATGTCCTGCCTCCTATTCTGCAGTGCCAGGCCGGGCACCTGGTG
  TGTAACCAATGCCGCCAGAAGTTGAGCTGCTGCCCGACGTGCAGGGGCGCCCTGACGCCC
  AGCATCAGGAACCTGGCTATGGAGAAGGTGGCCTCGGCAGTCCTGTTTCCCTGTAAGTAT
  GCCACCACGGGCTGTTCCCTGACCCTGCACCATACGGAGAAACCAGAACATGAAGACATA
  TGTGAATACCGTCCCTACTCCTGCCCATGTCCTGGTGCTTCCTGCAAGTGGCAGGGGTCC
  CTGGAAGCTGTGATGTCCCATCTCATGCACGCCCACAAGAGCATTACCACCCTTCAGGGA
  GAAGACATCGTCTTTCTAGCTACAGACATTAACTTGCCAGGGGCTGTCGACTGGGTGATG
  ATGCAGTCATGTTTTGGCCATCACTTCATGCTGGTGCTGGAGAAACAAGAGAAGTACGAA
  GGCCACCAGCAGTTTTTTGCCATCGTCCTGCTCATTGGCACCCGCAAGCAAGCCGAGAAC
  TTTGCCTACAGACTGGAGTTGAATGGGAACCGGCGGAGATTGACCTGGAAGGCCACGCCC
  CGTTCGATTCATGACGGTGTGGCTGCGGCCATCATGAACAGCGACTGCCTTGTTTTCGAC
  ACAGCCATAGCACATCTTTTTGCAGATAATGGGAACCTTGGAATCAATGTTACTATTTCT
  ACATGTTGTCCATGA
  
  Clone variation with respect to NM_005067.5
  829 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005067 unedited
  GCATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCTGCGCACCG
  CTGGGGTTCGGGGCCCGGGACGCCGCCGGGAGGAGGGCACCGCGCGGGGTCCGACGCGGA
  GGCGTGCTCGGAACGCCGGGGGCTGCGGAGTGCATCAGCGCGGTCCAGCCCTCCGCCTGC
  CGGGCGCCGAGCGTCTCCGCCGCCCGGACCTGGGCTGCGCGCCGTGGCGTTGCCTCGGAG
  CTCGCTGCCCGCGGGGCGCGCACCGCCTTGACCCGGGCGGCCCCGCGGCAGGCAGGCGCC
  CGCAGTTCCATGGTTGGTTCGGAGCGCGATGAGCCGCCCGTCCTCCACCGGCCCCAGCGC
  TAATAAACCCTGCAGCAAGCAGCCGCCGCCGCAGCCCCAGCACACTCCGTCCCCGGCTGC
  GCCCCCGGCCGCCGCCACCATCTCGGCTGCGGGCCCCGGCTCGTCCGCGGTGCCCGCCGC
  GGCGGCGGTGATCTCGGGCCCCGGCGGCGGCGGCGGNGCCGGCCCGGTGTCCCCGCAGCA
  CCACGAGCTGACCTCGCTCTTCGAGTGTCCGGTCTGCTTTGACTATGTCCTGCCTCCTAT
  TCTGCAGTGCCAGGCCGGGCACCTGGTGTGTAACCAATGCCGCCAGAAGTTGAGCTGCTG
  CCCGACGTGCAGGNGCGCCCTGACGCCCAGCATCAGGAACCTGGCTATGGAGAAGGTGGG
  CCTCGCAGTCCTGGTTTCCTGTTAGTATGCCACCACGGGCCTGTCTCCTGACCCTGCACC
  ATACCGGAGAACCAGAACATGAAGACATATGTGAATACCGTCCCTACTNCTGGCCATGTC
  CTGGTGCTTCCTGCAGATGGCAAGGGTCCCTGGAAGCTTGGATGTCCCATCTCATGCACG
  CCCACAANAGCATTACCACCCTTCAAGGGGGAGAACATCGTCTTCTAGCTACAGACATAA
  CTTGCCAGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005067 unedited
  GGTTTGGGGAAATAATTTATTAAAAAACTAGTGGAATGGTGGCAGNAAAAACAAAACCAA
  CACACAGCTTTAAATAGCAAGCATATGACACACCGGTTATAAAGCTTTTCCAACTCTACT
  GATTTAGTCTTTCACACCAGCTACTGTTGAGGAGGGCTTCCGAGGGAAGCCACGTGTAAA
  CGGGTCATCTGTACTAGTGGTGAGCAAAACTAATTACATGAGAACTATCAATGGCCATAT
  GACTTGCTTTCCTAGGCAATCCACCCAAAACATAGGTGAGTGGCCAAATCTCAGGCGTGC
  GTTCATGTGGATCTATAGTTCTAGTTTTAATTTGAGGGTTGGGGGAAGTTAGGGGATGAA
  ACAGTAGAAAGACAAACAGTATCGGCAGAGGGCAACCTTAAAGCACCTTTAAAATCTGGA
  CCCTTTCCCACAATTTAACCCAGTAATTCTCAAACATTACTGTTGAAAAGAATCACCTGA
  TGAGACTGTTAAATTGGCAGGCTTCCTAAATCTGCATTTTAAGATAAGCACCTCGGGTGA
  TTCAGTGTCTATTAGCCAGNCATTCAATTTCAGAAACACTGTTTAAAATGGATTANAACA
  ATTNCTACCTTCCCAAACCAAAGTAAGGGATCTGGTGACAGCACTGGGCTTTTGTCATGG
  CAGGGGACCATTTTGGAAAGCAGGCAAGAAAGGGCCTTCCATTCCTTACACACTGGATCA
  TATGAAGCCCTGTGCCACAACCTGGCAAGTGTTGTGTTAGGGGGTAAATCCAATTTAATA
  AGAGAGTGGTTTAATTACCAGAACAGTTCCACAATAACCACAGGGCAACCCCTTTATTCC
  CGGTTAATGAAACTTGTTTGGGCCCAAACCCCAGAGGACCCTGTTGTTTAAAAAAAAACG
  GGAATTGGGGGGTCCTGCCTGGGAGAACATTGGGAAAAAAACCTTT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_005067
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005067.5, NP_005058.3
• RefSeq Size: 2632 bp
• RefSeq ORF: 975 bp
• Locus ID: 6478
• Cytogenetics: 3q25.1
• Domains: Sina
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in regulating cellular response to hypoxia. [provided by RefSeq, Jul 2008]'