SIAH2 (NM_005067) Human Untagged Clone

CAT#: SC323731

SIAH2 (untagged)-Human seven in absentia homolog 2 (Drosophila) (SIAH2)


  "NM_005067" in other vectors (7)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol SIAH2
Synonyms hSiah2
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_005067.5 GGACGCGTGGGCGGACGCGTGGGCTGTTCCAGAAGCGGGTGGGCCAGGGCTCTGCGCACC
GCTGGGGTTCGGGGCCCGGGACGCCGCCGGGAGGAGGGCACCGCGCGGGGTCCGACGCGG
AGGCGTGCTCGGAACGCCGGGGGCTGCGGAGTGCATCAGCGCGGTCCAGCCCTCCGCCTG
CCGGGCGCCGAGCGTCTCCGCCGCCCGGACCTGGGCTGGGCGCCGTGGCGTTGCCTCGGA
GCTCGCTGCCCGCGGGGCGCGCACCGCCTTGACCCGGGCGGCCCCGCGGCAGGCAGGCGC
CCGCAGTTCCATGGTTGGTTCGGAGCGCGATGAGCCGCCCGTCCTCCACCGGCCCCAGCG
CTAATAAACCCTGCAGCAAGCAGCCGCCGCCGCAGCCCCAGCACACTCCGTCCCCGGCTG
CGCCCCCGGCCGCCGCCACCATCTCGGCTGCGGGCCCCGGCTCGTCCGCGGTGCCCGCCG
CGGCGGCGGTGATCTCGGGCCCCGGCGGCGGCGGCGGGGCCGGCCCGGTGTCCCCGCAGC
ACCACGAGCTGACCTCGCTCTTCGAGTGTCCGGTCTGCTTTGACTATGTCCTGCCTCCTA
TTCTGCAGTGCCAGGCCGGGCACCTGGTGTGTAACCAATGCCGCCAGAAGTTGAGCTGCT
GCCCGACGTGCAGGGGCGCCCTGACGCCCAGCATCAGGAACCTGGCTATGGAGAAGGTGG
CCTCGGCAGTCCTGTTTCCCTGTAAGTATGCCACCACGGGCTGTTCCCTGACCCTGCACC
ATACGGAGAAACCAGAACATGAAGACATATGTGAATACCGTCCCTACTCCTGCCCATGTC
CTGGTGCTTCCTGCAAGTGGCAGGGGTCCCTGGAAGCTGTGATGTCCCATCTCATGCACG
CCCACAAGAGCATTACCACCCTTCAGGGAGAAGACATCGTCTTTCTAGCTACAGACATTA
ACTTGCCAGGGGCTGTCGACTGGGTGATGATGCAGTCATGTTTTGGCCATCACTTCATGC
TGGTGCTGGAGAAACAAGAGAAGTACGAAGGCCACCAGCAGTTTTTTGCCATCGTCCTGC
TCATTGGCACCCGCAAGCAAGCCGAGAACTTTGCCTACAGACTGGAGTTGAATGGGAACC
GGCGGAGATTGACCTGGGAGGCCACGCCCCGTTCGATTCATGACGGTGTGGCTGCGGCCA
TCATGAACAGCGACTGCCTTGTTTTCGACACAGCCATAGCACATCTTTTTGCAGATAATG
GGAACCTTGGAATCAATGTTACTATTTCTACATGTTGTCCATGATGTGACTTTCGTAAAC
CTTCAAAATTATTTGGGCATAGTGCTCTATGTTTAATAAAGGTTTTTATAGATGTTTTAT
TCCATATGTCTTCACAAGTCAGGACCCACAATTACCCGTGTTTTGTTTGAACAGCAGTGT
CCCATCTGGCTTCGACCCAACAAAGTTCATTAACCTGGGATGAATGGGGTTGGCCTGTTG
GTGATTTGGATGCTGTTCTGTGATCTAAAACAACTCTTATTGAATTGTATTTACTCCCTA
AACAACACTTGACAGGCTGTTGCACAGGGCTTCTATAAGATCAGTGTGTTAGGAATGGGA
GGCCCCTTCCTGCCTGCCTTCCCATATTGGTCCCTTGACATTGACAAAAGCACAGTGACT
GTCAGCAGATTCCTTTACTTTTGTTTGTGGGAGGTAGGAATTGTTTTAATGCATTTTAAA
CAGTGTTTCTGAAATTGGATGGCTGGCTAATAGACACTGAATCACCCGGAGTGCTTATCT
TAAAATTGCAGATTTAGGGAGCCTGCCAATTTAACAGTCTCATCAGGTGATTCTTTTCAA
CAGTAATGTTTGAGAATTACTGGGTTAAATTGTGGGAAAGGGTCCAGATTTTAAAGGTGC
TTTAAGGTTGCCCTCTGCCGATACTGTTTGTCTTTCTACTGTTTCATCCCCTAACTTCCC
CCAACCCTCAAATTAAAACTAGAACTATAGATCCACATGAACGCACGCCTGAGATTTGGC
CACTCACCTATGTTTTGGGTGGATTGCCTAGGAAAGCAAGTCATATGGCCATTGATAGTT
CTCATGTAATTAGTTTTGCTCACCACTAGTACAGATGACCCGTTTACACGTGGCTTCCCT
CGGAAGCCCTCCTCAACAGTAGCTGGTGTGAAAGACTAAATCAGTAGAGTTGGAAAAGCT
TTATAACCGGTGTGTCATATGCTTGCTATTTAAAGCTGTGTGTTGGTTTTGTTTTTCTGC
CACATTCACTAGTTTTTTAATAAATATTTTCCAAAAATGAAAAAAAAAAAAAAAAAAAAA
AAAAAAA
Restriction Sites ECoRI-NOT     
ACCN NM_005067
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005067.5, NP_005058.3
RefSeq Size 2632 bp
RefSeq ORF 975 bp
Locus ID 6478
Cytogenetics 3q25.1
Domains Sina
Protein Families Druggable Genome, Transcription Factors
Gene Summary 'This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in regulating cellular response to hypoxia. [provided by RefSeq, Jul 2008]'

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.