Neuroserpin (SERPINI1) (NM_005025) Human Untagged Clone

CAT#: SC117018

SERPINI1 (untagged)-Human serpin peptidase inhibitor, clade I (neuroserpin), member 1 (SERPINI1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SERPINI1
• Synonyms: neuroserpin; PI12
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117018 sequence for NM_005025 edited (data generated by NextGen Sequencing)
  ATGGCTTTCCTTGGACTCTTCTCTTTGCTGGTTCTGCAAAGTATGGCTACAGGGGCCACT
  TTCCCTGAGGAAGCCATTGCTGACTTGTCAGTGAATATGTATAATCGTCTTAGAGCCACT
  GGTGAAGATGAAAATATTCTCTTCTCTCCATTGAGTATTGCTCTTGCAATGGGAATGATG
  GAACTTGGGGCCCAAGGATCTACCCAGAAAGAAATCCGCCACTCAATGGGATATGACAGC
  CTAAAAAATGGTGAAGAATTTTCTTTCTTGAAGGAGTTTTCAAACATGGTAACTGCTAAA
  GAGAGCCAATATGTGATGAAAATTGCCAATTCCTTGTTTGTGCAAAATGGATTTCATGTC
  AATGAGGAGTTTTTGCAAATGATGAAAAAATATTTTAATGCAGCAGTAAATCATGTGGAC
  TTCAGTCAAAATGTAGCCGTGGCCAACTACATCAATAAGTGGGTGGAGAATAACACAAAC
  AATCTGGTGAAAGATTTGGTATCCCCAAGGGATTTTGATGCTGCCACTTATCTGGCCCTC
  ATTAATGCTGTCTATTTCAAGGGGAACTGGAAGTCGCAGTTTAGGCCTAAAAATACTAGA
  ACCTTTTCTTTCACTAAAGATGATGAAAGTGAAGTCCAAATTCCAATGATGTATCAGCAA
  GGAGAATTTTATTATGGGGAATTTAGTGATGGCTCCAATGAAGCTGGTGGTATCTACCAA
  GTCCTAGAAATACCATATGAAGGAGATGAAATAAGCATGATGCTGGTGCTGTCCAGACAG
  GAAGTTCCTCTTGCTACTCTGGAGCCATTAGTCAAAGCACAGCTGGTTGAAGAATGGGCA
  AACTCTGTGAAGAAGCAAAAAGTAGAAGTATACCTGCCCAGGTTCACAGTGGAACAGGAA
  ATTGATTTAAAAGATGTTTTGAAGGCTCTTGGAATAACTGAAATTTTCATCAAAGATGCA
  AATTTGACAGGCCTCTCTGATAATAAGGAGATTTTTCTTTCCAAAGCAATTCACAAGTCC
  TTCCTAGAGGTTAATGAAGAAGGCTCAGAAGCTGCTGCTGTCTCAGGAATGATTGCAATT
  AGTAGGATGGCTGTGCTGTATCCTCAAGTTATTGTCGACCATCCATTTTTCTTTCTTATC
  AGAAACAGGAGAACTGGTACAATTCTATTCATGGGACGAGTCATGCATCCTGAAACAATG
  AACACAAGTGGACATGATTTCGAAGAACTTTAA
  
  Clone variation with respect to NM_005025.4
  589 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005025 unedited
  TTACGTTTTGTAACCGACCTCNTATAGGCGTCCGCGACATTCGCACGAGGGCGGAGCGGA
  TCACATTCCGCCGAGCACAAGCTCCAGCATCCCGTCAGGGGTTGCAGGTGTGTGGGAGGC
  TTGAAACTGTTACAATATGGCTTTCCTTGGACTCTTCTCTTTGCTGGTTCTGCAAAGTAT
  GGCTACAGGGGCCACTTTCCCTGAGGAAGCCATTGCTGACTTGTCAGTGAATATGTATAA
  TCGTCTTATAGCCACTGGTGAAGATGAAAATATTCTCTTCTCTCCATTGAGTATTGCTCT
  TGCAATGGGAATGATGGAACTTGGGGCCCAAGGATCTACCCAGAAAGAAATCCGCCACTC
  AATGGGATATGACAGCCTAAAAAATGGTGAAGAATTTTCTTTCTTGAAGGAGTTTTCAAA
  CATGGTAACTGCTAAAGAGAGCCAATATGTGATGAAAATTGCCAATTCCTTGTTTGTGCA
  AAATGGATTTCATGTCAATGAGGAGTTTTTGCAAATGATGAAAAAATATTTTAATGCAGC
  AGTAAATCATGTGGACTTCAGTCAAAATGTAGCCGTGGCCAACTACATCAATAAGTGGGT
  GGAGAATAACACAAACAATCTGGTGAAAGATTTGGTATCCCCAATGGATTTTGATGCTGC
  CACTTATCTGGCCCTCATTAATGCTGTCTATTTCAAGGNGAACTGGAAGTCGCAGTTTAT
  GCCTAAAAATACTAGAACCTTTTCTTTCACTATAGATGATGAAAGTGAAAGTCAAATTCC
  AATGATGTATCAGCAAGGAGAATTTTATTATGGGNGAATCTAGTGATGGCTCCAATGAAG
  CTGNTGGTATCTACCAAGTCCTATNAATACATATGAANGAGATGAAATAGCATGATGCTG
  GTGCTGTCCAGACAGGAAGTNCCTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005025 unedited
  NNNTTTCGCTCTGGACCCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTCA
  ATAGGTACTTTTATTTTAAACAACAGCACACAAACACAATGACATAACAGGACCATTAGC
  TTAATACTGATAACATTCCTTGACAAGTTACTTATAATTTACATATATTGTTTTTATCTG
  GAACTATTTTAAATATTATCTTAAGATATACTGTAAAACACAAATCCTAAATATATACCA
  GTTGCAAACATAATGTGCTTAGTTACTGTTTTCCTTGTTATTCAAATAAAGTAACTTAAA
  GTTCTTCGAAATCATGTCCACTTGTGTTCATTGTTTCAGGATGCATGACTCGTCCCATGA
  ATAGAATTGTACCAGTTCTCCTGTTTCTGATAAGAAAGAAAAATGGATGGTCGACAATAA
  CTTGAGGATACAGCACAGCCATCCTACTAATTGCAATCATTCCTGAGACAGCAGCAGCTT
  CTGAGCCTTCTTCATTAACCTCTAGGAAGGACTTGTGAATTGCTTTGGAAAGAAAAATCT
  CCTTATTATCAGAGAGGCCTGTCAAATTTGCATCTTTGATGAAAATTTCAGTTATTCCAA
  GAGCCTTCAAAACATCTTTTAAATCAATTTCCTGTTCCACTGTGAACCTGGGCAGGTATA
  CTTCTACTTTNTGCTTCTTACAGAGTTTGCCCATTCTTCAACCAGCTGTGCTTTGACTAA
  TGGCTCCAGAGTAGCAAGAAGAAACTTCCTGTCTGGACAGCACCAGCATCATGCTTATTT
  CATCTCCTTCATATGGNATTCCTAGGAACTGGGTAGATACACCCAGCTCCATTGAAGCCA
  TCACTAANATCCCCATATANAATTCTCCTTGCTGATCCATACTGGAAATTGGNACTCACC
  TCTATCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005025
• Insert Size: 1430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005025.2, NP_005016.1
• RefSeq Size: 1910 bp
• RefSeq ORF: 1233 bp
• Locus ID: 5274
• Cytogenetics: 3q26.1
• Domains: SERPIN
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The protein is primarily secreted by axons in the brain, and preferentially reacts with and inhibits tissue-type plasminogen activator. It is thought to play a role in the regulation of axonal growth and the development of synaptic plasticity. Mutations in this gene result in familial encephalopathy with neuroserpin inclusion bodies (FENIB), which is a dominantly inherited form of familial encephalopathy and epilepsy characterized by the accumulation of mutant neuroserpin polymers. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same isoform.