TIS11B (ZFP36L1) (NM_004926) Human Untagged Clone

CAT#: SC117032

ZFP36L1 (untagged)-Human zinc finger protein 36, C3H type-like 1 (ZFP36L1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ZFP36L1
• Synonyms: Berg36; BRF1; cMG1; ERF-1; ERF1; RNF162B; TIS11B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004926, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACCACCACCCTCGTGTCTGCCACCATCTTCGACTTGAGCGAAGTTTTATGCAAGGGTAACAAGATGC
  TCAACTATAGTGCTCCCAGTGCAGGGGGTTGCCTGCTGGACAGAAAGGCAGTGGGCACCCCTGCTGGTGG
  GGGCTTCCCTCGGAGGCACTCAGTCACCCTGCCCAGCTCCAAGTTCCACCAGAACCAGCTCCTCAGCAGC
  CTCAAGGGTGAGCCAGCCCCCGCTCTGAGCTCGCGAGACAGCCGCTTCCGAGACCGCTCCTTCTCGGAAG
  GGGGCGAGCGGCTGCTGCCCACCCAGAAGCAGCCCGGGGGCGGCCAGGTCAACTCCAGCCGCTACAAGAC
  GGAGCTGTGCCGCCCCTTTGAGGAAAACGGTGCCTGTAAGTACGGGGACAAGTGCCAGTTCGCACACGGC
  ATCCACGAGCTCCGCAGCCTGACCCGCCACCCCAAGTACAAGACGGAGCTGTGCCGCACCTTCCACACCA
  TCGGCTTTTGCCCCTACGGGCCCCGCTGCCACTTCATCCACAACGCTGAAGAGCGCCGTGCCCTGGCCGG
  GGCCCGGGACCTCTCCGCTGACCGTCCCCGCCTCCAGCATAGCTTTAGCTTTGCTGGGTTTCCCAGTGCC
  GCTGCCACCGCCGCTGCCACCGGGCTGCTGGACAGCCCCACGTCCATCACCCCACCCCCTATTCTGAGCG
  CCGATGACCTCCTGGGCTCACCTACCCTGCCCGATGGCACCAATAACCCTTTTGCCTTCTCCAGCCAGGA
  GCTGGCAAGCCTCTTTGCCCCTAGCATGGGGCTGCCCGGGGGTGGCTCCCCGACCACCTTCCTCTTCCGG
  CCCATGTCCGAGTCCCCTCACATGTTTGACTCTCCCCCCAGCCCTCAGGATTCTCTCTCGGACCAGGAGG
  GCTACCTGAGCAGCTCCAGCAGCAGCCACAGTGGCTCAGACTCCCCGACCTTGGACAACTCAAGACGCCT
  GCCCATCTTCAGCAGACTTTCCATCTCAGATGACTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004926 unedited
  NTGTCAGAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCGAGGGG
  CGCCGGGAACTGGCGTGTGGGACTCCAGACAGGAGAGGCTGCGCCTTCCCCGCACCGGGA
  CCTTCGCGACACACCAGATCCTCGCCCCTGGCTCGCGCGAACTCACAGGATGACCACCAC
  CCTCGTGTCTGCCACCATCTTCGACTTGAGCGAAGTTTTATGCAAGGGTAACAAGATGCT
  CAACTATAGTGCTCCCAGTGCAGGGGGTTGCCTGCTGGACAGAAAGGCAGTGGGCACCCC
  TGCTGGTGGGGGCTTCCCTCGGAGGCACTCAGTCACCCTGCCCAGCTCCAAGTTCCACCA
  GAACCAGCTCCTCAGCAGCCTCAAGGGTGAGCCAGCCCCCGCTCTGAGCTCGCGAGACAG
  CCGCTTCCGAGACCGCTCCTTCTCGGAAGGGGGCGAGCGGCTGCTGCCCACCCAGAAGCA
  GCCCGGGGGCGGCCAGGTCAACTCCAGCCGCTACAAGACGGAGCTGTGCCGCCCCTTTGA
  GGAAAACGGTGCCTGTAAGTACGGGGACAAGTGCCAGTTCGCACACGGCATCCACGAGCT
  CCGCAGCCTGACCCGCCACCCCAAGTACAAGACGGAGCTGTGCCGCACCTTCCACACCAT
  CGGCTTTTGCCCCTACGGGCCCCGCTGCCACTTCATCCACAACGCTGAAGAGCGCCGTGC
  CCTGGCCGGNGCCCGGGACCTCTCCGCTGACCGTCCCCGCCTCCAGCATAGCTNTAGCTN
  TGCTGGGTTTCCCAGTGCCGCTGCCACCGCCGCTGCCACCGGGCTGCTGGACAGCNCCAC
  GTNCATCACCCCACCCCCTATTCTGAGCGCCGATGACCTCCTGGGCTCACCTACCCTGCC
  CGATGGCACCNATACCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004926 unedited
  CGCCGCATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTTGAAAAAACGGGGTTTATTGA
  TTTTTAAACTGCAAATAGTCGTTACAAAAAGTTTTTTTTTCTTTTAAATAAATTCACACA
  AAGAAAGAGAAATAGAAAGCGACGGTAGTGACCAGCAAGAGGAATAATAATTACATTCAT
  CTTAATGTGTGTGTGCCAGTTCTGTTTACATTAACATTGGAAAACTCCAGACCTGGAATC
  CAGAACCTCAAATCTGTGAGTGGAATGTCTTGAGATGGGCACGTGGAAGTCAAAGGGTTT
  CTCTTTTTTTTTTTTTCCCCTTTTAAAAGCTATACATAAAAAGTTGTTTTCCTTCTGTAC
  TGTCACAGAACTTTTACATACATTCTCAGTCCTAGTTGTGAAAGGCCTAAAGAGAAAGAA
  ACTCATTTTGCAGTCCAACACAAAGGGGGGATTTTCTAAAATAAATAATCCAACAGTTTT
  TTGCATTTTTTTAAATTAATTCTTCATTTTTTTAAAATAAAATACCCAAAAAAGGGCAAA
  GTCACAAAAAATGTCGTTGAAAATTAATATATTAAAACTGTGGAAAAAAAGGATAAAGCC
  CCCCCCAAAATCTTACGACTATTATGAAGATCATAATTAACCATAAAAGAATATTTCCCC
  TAGGATTTGCCTCCCCGGTTATTATGAACAAACTTACAAAAAAAAGCTTAGTTGGCGCAA
  AAAACCGTTCGATAAGCAAAAAACCCTTTTTTTTTTGTCGGGCCAGGGCAAGCCAAATTT
  TGCTCTCAAATTCCGAAGTTTATGAAGGTTAACCCCAGGAAAAACCTGGGGGGAGGCTCT
  AATGATAACCTCGGCTTCCTAACAAAACCTTAAAGCTCTTCTACGGAAATGTTACACCAA
  TTTGGTTGGGAAGGATACCTAAACCGCCCACCTAATCTTTTTTTTAACTTTCTCCATTTT
  TTTTGGGGCATTCTTTAAGAAGGAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004926
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004926.2, NP_004917.2
• RefSeq Size: 3022 bp
• RefSeq ORF: 1017 bp
• Locus ID: 677
• Cytogenetics: 14q24.1
• Domains: zf-CCCH, Tis11B_C
• Protein Families: Transcription Factors
• Gene Summary: 'This gene is a member of the TIS11 family of early response genes, which are induced by various agonists such as the phorbol ester TPA and the polypeptide mitogen EGF. This gene is well conserved across species and has a promoter that contains motifs seen in other early-response genes. The encoded protein contains a distinguishing putative zinc finger domain with a repeating cys-his motif. This putative nuclear transcription factor most likely functions in regulating the response to growth factors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]'
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1. Variants 1 and 2 encode the same isoform.