DDX1 (NM_004939) Human Untagged Clone

CAT#: SC117041

DDX1 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (DDX1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX1
• Synonyms: DBP-RB; UKVH5d
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117041 sequence for NM_004939 edited (data generated by NextGen Sequencing)
  ATGGCGGCCTTCTCCGAGATGGGTGTAATGCCTGAGATTGCACAAGCTGTGGAAGAGATG
  GATTGGCTCCTCCCAACTGATATCCAGGCTGAATCTATCCCATTGATCTTAGGAGGAGGT
  GATGTACTTATGGCTGCAGAAACAGGAAGTGGCAAAACTGGTGCTTTTAGTATTCCAGTT
  ATCCAGATAGTTTATGAAACTCTGAAAGACCAACAGGAAGGCAAAAAAGGAAAAACAACA
  ATTAAAACTGGTGCTTCAGTGCTGAACAAATGGCAGATGAACCCATATGACAGAGGATCT
  GCTTTTGCAATTGGGTCAGATGGTCTTTGTTGTCAAAGCAGAGAAGTAAAGGAATGGCAT
  GGGTGTAGAGCTACTAAAGGATTAATGAAAGGGAAACACTACTATGAAGTATCCTGTCAT
  GACCAAGGGTTATGCAGGGTCGGGTGGTCTACCATGCAGGCCTCTTTGGACCTAGGTACT
  GACAAGTTTGGATTTGGCTTTGGTGGAACAGGAAAGAAATCCCATAACAAACAATTTGAT
  AATTATGGAGAGGAATTCACTATGCATGATACCATTGGATGTTACCTGGATATAGATAAG
  GGACATGTCAAGTTCTCCAAAAATGGAAAAGATCTTGGTCTGGCATTTGAAATACCACCA
  CATATGAAAAACCAAGCCCTCTTTCCTGCCTGTGTTTTGAAGAATGCTGAACTGAAATTT
  AACTTCGGTGAAGAGGAATTTAAGTTTCCACCAAAAGATGGCTTTGTTGCTCTTTCCAAG
  GCACCGGATGGTTACATTGTCAAATCACAGCACTCAGGTAATGCACAGGTGACACAAACA
  AAGTTTCTCCCCAATGCTCCGAAAGCTCTCATTGTTGAACCTTCCCGGGAGTTAGCTGAA
  CAAACTTTGAATAACATCAAGCAGTTTAAGAAATACATTGATAATCCTAAATTAAGGGAG
  CTTCTGATAATTGGAGGTGTTGCAGCCCGGGATCAGCTCTCTGTTTTGGAAAATGGAGTA
  GATATAGTTGTAGGTACTCCGGGAAGACTAGATGACTTGGTGTCAACTGGAAAGCTGAAC
  TTATCTCAAGTTAGATTCCTGGTCCTGGATGAAGCTGATGGGCTTCTTTCTCAAGGTTAT
  TCTGATTTTATAAATAGGATGCACAATCAGATTCCTCAGGTTACCTCTGATGGAAAAAGA
  CTTCAGGTGATTGTTTGCTCTGCCACTTTGCATTCTTTCGATGTAAAGAAACTGTCCGAG
  AAGATAATGCATTTTCCTACATGGGTTGACTTAAAAGGAGAAGACTCTGTTCCAGATACT
  GTACACCATGTTGTTGTCCCAGTAAATCCCAAAACTGACAGACTCTGGGAAAGGCTTGGA
  AAGAGCCACATTAGAACTGATGATGTACATGCAAAAGATAACACAAGACCTGGTGCTAAT
  AGTCCAGAGATGTGGTCTGAAGCTATTAAAATCCTGAAAGGGGAGTATGCTGTCCGGGCA
  ATCAAGGAACATAAGATGGATCAAGCAATTATCTTCTGTAGAACCAAAATTGACTGTGAT
  AACTTGGAGCAGTACTTTATACAACAAGGAGGAGGACCTGATAAAAAAGGACACCAGTTC
  TCATGTGTTTGTCTTCATGGTGACAGAAAGCCTCATGAGAGAAAGCAAAACTTGGAAAGA
  TTTAAGAAAGGAGATGTAAGATTCTTGATTTGCACAGATGTAGCTGCTAGAGGAATTGAT
  ATCCACGGTGTTCCTTATGTTATAAATGTCACTCTGCCCGATGAAAAGCAAAACTACGTA
  CATCGAATTGGCAGAGTAGGAAGAGCTGAAAGGATGGGTCTGGCAATTTCCCTGGTGGCA
  ACAGAAAAAGAAAAGGTTTGGTACCATGTATGTAGCAGCCGTGGAAAAGGGTGTTATAAC
  ACAAGACTCAAGGAAGATGGAGGCTGTACCATATGGTACAACGAGATGCAGTTACTATCT
  GAGATAGAAGAACACCTGAACTGTACCATTTCTCAGGTTGAGCCGGATATAAAGGTACCA
  GTGGATGAATTTGATGGGAAAGTTACCTACGGTCAGAAAAGGGCTGCTGGTGGTGGAAGC
  TATAAAGGCCATGTGGATATTTTGGCACCTACTGTTCAAGAGTTGGCTGCCCTTGAAAAG
  GAGGCGCAGACATCTTTCCTGCATCTTGGCTACCTTCCTAACCAGCTGTTCAGAACCTTC
  TGA
  
  Clone variation with respect to NM_004939.1
  912 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004939 unedited
  ATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGCGCGCCGCCACTGCCGCGCCG
  TGTCAGTCGGGAGGGAGGGAGCGAGCAGGCGAAGCCGCGGAGGACGGGGTGAAGATGGCG
  GCCTTCTCCGAGATGGGTGTAATGCCTGAGATTGCACAAGCTGTGGAAGAGATGGATTGG
  CTCCTCCCAACTGATATCCAGGCTGAATCTATCCCATTGATCTTAGGAGGAGGTGATGTA
  CTTATGGCTGCAGAAACAGGAAGTGGCAAAACTGGTGCTTTTAGTATTCCAGTTATCCAG
  ATAGTTTATGAAACTCTGAAAGACCAACAGGAAGGCAAAAAAGGAAAAACAACAATTAAA
  ACTGGTGCTTCAGTGCTGAACAAATGGCAGATGAACCCATATGACAGAGGATCTGCTTTT
  GCAATTGGGTCAGATGGTCTTTGTTGTCAAAGCAGAGAAGTAAAGGAATGGCATGGGTGT
  AGAGCTACTAAAGGATTAATGAAAGGGAAACACTACTATGAAGTATCCTGTCATGACCAA
  GGGTTATGCAGGGTCGGGTGGTCTACCATGCANGCCTCTNTGGACCTANGTACTGACNNA
  GTTGGATTTGGCTTTGGTGGAACANGGAAAGAATCCNATNNACAACANNTTTGATATTAT
  GGAGAGGAATTCACTATGCATGATACCATTGGNATGTACCTGGATATAGATAAGGGACAT
  GTCCAGTTCTCCAAAAATGGAAAAGATCTTGGTCTGGCATTTGANATAACCACCACATAT
  GAAAAACCCAGCCCCTCTTTTCTGCCTGTGTTTTGAAGAATGCTGAACTGAATTTTAACT
  NCGGTGAANAAGGAATTTAAGTTTNCCACAAAAGATGGCTTNGNTGCTCTTTTCCAGCAC
  CGTATGTTTACATGTCAATCCCACACTCAGGTATGCACAGTGACCCAACAAGTTTCTCCC
  ATGCCTCGAGCTCTATGNTGGACTTCCGGNAGTACTGACAACTTGATACTCAGCAGTAGA
  ATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004939 unedited
  TATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTATAGTCATCAA
  ATTTATTTTCATTGGGATGCCATTTTTTGAAGAATTCCTAAGACTAATGTTTCTTGACAT
  GCAAGAGTTAGCATTAATAGCTTACGTTACTATAAATACTGCTGCTTGGAAGCAGTACAA
  CTGTTTTAGAGTTTTAAGACTACAGACTTTCATTACTCAAATCTTATTCAGCAAATGTAA
  AAATCAGAAGGTTCTGAACAGCTGGTTAGGAAGGTAGCCAAGATGCAGGAAAGATGTCTG
  CGCCTCCTTTTCAAGGGCAGCCAACTCTTGAACAGTAGGTGCCAAAATATCCACATGGCC
  TTTATAGCTTCCACCACCAGCAGCCCTTTTCTGACCGTAGGTAACTTTCCCATCAAATTC
  ATCCACTGGTACCTTTATATCCGGCTCAACCTGAGAAATGGTACAGTTCAGGTGTTCTTC
  TATCTCAGATAGTAACTGCATCTCGTTGTACCATATGGTACAGCCTCCATCTTCCTTGAG
  TCTTGTGTTATAACACCCTTTTCCACGGCTGCTACATACATGGTACCAAACCTTTTCTTT
  TTCTGTTGCCACCAGGGAAATTGCCAGACCCATCCTTTCAGCTCTTCCTACTCTGCCAAT
  TCGATGTACGTAGTTNTGCTTTTCATCGCCTAGAGTGACATTTATAACATAAGGGACACC
  CGGGGATATCAATTCCTCTAGCAGCTACATCTGTGCAAATCAAGAAATCTACATCTCCTA
  CTTTTAATCTTCCCAAGTTTTGCTTTCTCTCATGAGGCTTTCTGTACCATGAAGACAACA
  CTGAGAACTGTGTCTTTTTATCAGGCCTCTCCTTGTGATCAAGACTGCTCCAGTTATACA
  TCATTTTGTCTACAGAGATAATGTTGACCATTTAGTCCTTGATGCCGGAGCTACTCCCTT
  TAGATTTAATGCTTAACCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004939
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004939.1, NP_004930.1
• RefSeq Size: 2706 bp
• RefSeq ORF: 2223 bp
• Locus ID: 1653
• Cytogenetics: 2p24.3
• Domains: DEAD, helicase_C, SPRY
• Protein Families: Druggable Genome
• Gene Summary: 'DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin. [provided by RefSeq, Jul 2008]'