DDX1 (NM_004939) Human Untagged Clone

CAT#: SC117041

DDX1 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (DDX1)


  "NM_004939" in other vectors (6)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol DDX1
Synonyms DBP-RB; UKVH5d
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC117041 sequence for NM_004939 edited (data generated by NextGen Sequencing)
ATGGCGGCCTTCTCCGAGATGGGTGTAATGCCTGAGATTGCACAAGCTGTGGAAGAGATG
GATTGGCTCCTCCCAACTGATATCCAGGCTGAATCTATCCCATTGATCTTAGGAGGAGGT
GATGTACTTATGGCTGCAGAAACAGGAAGTGGCAAAACTGGTGCTTTTAGTATTCCAGTT
ATCCAGATAGTTTATGAAACTCTGAAAGACCAACAGGAAGGCAAAAAAGGAAAAACAACA
ATTAAAACTGGTGCTTCAGTGCTGAACAAATGGCAGATGAACCCATATGACAGAGGATCT
GCTTTTGCAATTGGGTCAGATGGTCTTTGTTGTCAAAGCAGAGAAGTAAAGGAATGGCAT
GGGTGTAGAGCTACTAAAGGATTAATGAAAGGGAAACACTACTATGAAGTATCCTGTCAT
GACCAAGGGTTATGCAGGGTCGGGTGGTCTACCATGCAGGCCTCTTTGGACCTAGGTACT
GACAAGTTTGGATTTGGCTTTGGTGGAACAGGAAAGAAATCCCATAACAAACAATTTGAT
AATTATGGAGAGGAATTCACTATGCATGATACCATTGGATGTTACCTGGATATAGATAAG
GGACATGTCAAGTTCTCCAAAAATGGAAAAGATCTTGGTCTGGCATTTGAAATACCACCA
CATATGAAAAACCAAGCCCTCTTTCCTGCCTGTGTTTTGAAGAATGCTGAACTGAAATTT
AACTTCGGTGAAGAGGAATTTAAGTTTCCACCAAAAGATGGCTTTGTTGCTCTTTCCAAG
GCACCGGATGGTTACATTGTCAAATCACAGCACTCAGGTAATGCACAGGTGACACAAACA
AAGTTTCTCCCCAATGCTCCGAAAGCTCTCATTGTTGAACCTTCCCGGGAGTTAGCTGAA
CAAACTTTGAATAACATCAAGCAGTTTAAGAAATACATTGATAATCCTAAATTAAGGGAG
CTTCTGATAATTGGAGGTGTTGCAGCCCGGGATCAGCTCTCTGTTTTGGAAAATGGAGTA
GATATAGTTGTAGGTACTCCGGGAAGACTAGATGACTTGGTGTCAACTGGAAAGCTGAAC
TTATCTCAAGTTAGATTCCTGGTCCTGGATGAAGCTGATGGGCTTCTTTCTCAAGGTTAT
TCTGATTTTATAAATAGGATGCACAATCAGATTCCTCAGGTTACCTCTGATGGAAAAAGA
CTTCAGGTGATTGTTTGCTCTGCCACTTTGCATTCTTTCGATGTAAAGAAACTGTCCGAG
AAGATAATGCATTTTCCTACATGGGTTGACTTAAAAGGAGAAGACTCTGTTCCAGATACT
GTACACCATGTTGTTGTCCCAGTAAATCCCAAAACTGACAGACTCTGGGAAAGGCTTGGA
AAGAGCCACATTAGAACTGATGATGTACATGCAAAAGATAACACAAGACCTGGTGCTAAT
AGTCCAGAGATGTGGTCTGAAGCTATTAAAATCCTGAAAGGGGAGTATGCTGTCCGGGCA
ATCAAGGAACATAAGATGGATCAAGCAATTATCTTCTGTAGAACCAAAATTGACTGTGAT
AACTTGGAGCAGTACTTTATACAACAAGGAGGAGGACCTGATAAAAAAGGACACCAGTTC
TCATGTGTTTGTCTTCATGGTGACAGAAAGCCTCATGAGAGAAAGCAAAACTTGGAAAGA
TTTAAGAAAGGAGATGTAAGATTCTTGATTTGCACAGATGTAGCTGCTAGAGGAATTGAT
ATCCACGGTGTTCCTTATGTTATAAATGTCACTCTGCCCGATGAAAAGCAAAACTACGTA
CATCGAATTGGCAGAGTAGGAAGAGCTGAAAGGATGGGTCTGGCAATTTCCCTGGTGGCA
ACAGAAAAAGAAAAGGTTTGGTACCATGTATGTAGCAGCCGTGGAAAAGGGTGTTATAAC
ACAAGACTCAAGGAAGATGGAGGCTGTACCATATGGTACAACGAGATGCAGTTACTATCT
GAGATAGAAGAACACCTGAACTGTACCATTTCTCAGGTTGAGCCGGATATAAAGGTACCA
GTGGATGAATTTGATGGGAAAGTTACCTACGGTCAGAAAAGGGCTGCTGGTGGTGGAAGC
TATAAAGGCCATGTGGATATTTTGGCACCTACTGTTCAAGAGTTGGCTGCCCTTGAAAAG
GAGGCGCAGACATCTTTCCTGCATCTTGGCTACCTTCCTAACCAGCTGTTCAGAACCTTC
TGA

Clone variation with respect to NM_004939.1
912 c=>t
>OriGene 5' read for NM_004939 unedited
ATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGCGCGCCGCCACTGCCGCGCCG
TGTCAGTCGGGAGGGAGGGAGCGAGCAGGCGAAGCCGCGGAGGACGGGGTGAAGATGGCG
GCCTTCTCCGAGATGGGTGTAATGCCTGAGATTGCACAAGCTGTGGAAGAGATGGATTGG
CTCCTCCCAACTGATATCCAGGCTGAATCTATCCCATTGATCTTAGGAGGAGGTGATGTA
CTTATGGCTGCAGAAACAGGAAGTGGCAAAACTGGTGCTTTTAGTATTCCAGTTATCCAG
ATAGTTTATGAAACTCTGAAAGACCAACAGGAAGGCAAAAAAGGAAAAACAACAATTAAA
ACTGGTGCTTCAGTGCTGAACAAATGGCAGATGAACCCATATGACAGAGGATCTGCTTTT
GCAATTGGGTCAGATGGTCTTTGTTGTCAAAGCAGAGAAGTAAAGGAATGGCATGGGTGT
AGAGCTACTAAAGGATTAATGAAAGGGAAACACTACTATGAAGTATCCTGTCATGACCAA
GGGTTATGCAGGGTCGGGTGGTCTACCATGCANGCCTCTNTGGACCTANGTACTGACNNA
GTTGGATTTGGCTTTGGTGGAACANGGAAAGAATCCNATNNACAACANNTTTGATATTAT
GGAGAGGAATTCACTATGCATGATACCATTGGNATGTACCTGGATATAGATAAGGGACAT
GTCCAGTTCTCCAAAAATGGAAAAGATCTTGGTCTGGCATTTGANATAACCACCACATAT
GAAAAACCCAGCCCCTCTTTTCTGCCTGTGTTTTGAAGAATGCTGAACTGAATTTTAACT
NCGGTGAANAAGGAATTTAAGTTTNCCACAAAAGATGGCTTNGNTGCTCTTTTCCAGCAC
CGTATGTTTACATGTCAATCCCACACTCAGGTATGCACAGTGACCCAACAAGTTTCTCCC
ATGCCTCGAGCTCTATGNTGGACTTCCGGNAGTACTGACAACTTGATACTCAGCAGTAGA
ATC
>OriGene 3' read for NM_004939 unedited
TATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTATAGTCATCAA
ATTTATTTTCATTGGGATGCCATTTTTTGAAGAATTCCTAAGACTAATGTTTCTTGACAT
GCAAGAGTTAGCATTAATAGCTTACGTTACTATAAATACTGCTGCTTGGAAGCAGTACAA
CTGTTTTAGAGTTTTAAGACTACAGACTTTCATTACTCAAATCTTATTCAGCAAATGTAA
AAATCAGAAGGTTCTGAACAGCTGGTTAGGAAGGTAGCCAAGATGCAGGAAAGATGTCTG
CGCCTCCTTTTCAAGGGCAGCCAACTCTTGAACAGTAGGTGCCAAAATATCCACATGGCC
TTTATAGCTTCCACCACCAGCAGCCCTTTTCTGACCGTAGGTAACTTTCCCATCAAATTC
ATCCACTGGTACCTTTATATCCGGCTCAACCTGAGAAATGGTACAGTTCAGGTGTTCTTC
TATCTCAGATAGTAACTGCATCTCGTTGTACCATATGGTACAGCCTCCATCTTCCTTGAG
TCTTGTGTTATAACACCCTTTTCCACGGCTGCTACATACATGGTACCAAACCTTTTCTTT
TTCTGTTGCCACCAGGGAAATTGCCAGACCCATCCTTTCAGCTCTTCCTACTCTGCCAAT
TCGATGTACGTAGTTNTGCTTTTCATCGCCTAGAGTGACATTTATAACATAAGGGACACC
CGGGGATATCAATTCCTCTAGCAGCTACATCTGTGCAAATCAAGAAATCTACATCTCCTA
CTTTTAATCTTCCCAAGTTTTGCTTTCTCTCATGAGGCTTTCTGTACCATGAAGACAACA
CTGAGAACTGTGTCTTTTTATCAGGCCTCTCCTTGTGATCAAGACTGCTCCAGTTATACA
TCATTTTGTCTACAGAGATAATGTTGACCATTTAGTCCTTGATGCCGGAGCTACTCCCTT
TAGATTTAATGCTTAACCC
Restriction Sites NotI-NotI     
ACCN NM_004939
Insert Size 2500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004939.1, NP_004930.1
RefSeq Size 2706 bp
RefSeq ORF 2223 bp
Locus ID 1653
Cytogenetics 2p24.3
Domains DEAD, helicase_C, SPRY
Protein Families Druggable Genome
Gene Summary 'DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin. [provided by RefSeq, Jul 2008]'

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