Dynamin 2 (DNM2) (NM_004945) Human Untagged Clone

CAT#: SC117045

DNM2 (untagged)-Human dynamin 2 (DNM2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNM2
• Synonyms: CMT2M; CMTDI1; CMTDIB; DI-CMTB; DYN2; DYNII; LCCS5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004945, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCAACCGCGGGATGGAAGAGCTGATCCCGCTGGTCAACAAACTGCAGGACGCCTTCAGCTCCATCG
  GCCAGAGCTGCCACCTGGACCTGCCGCAGATCGCTGTAGTGGGCGGCCAGAGCGCCGGCAAGAGCTCGGT
  GCTGGAGAACTTCGTGGGCCGGGACTTCCTTCCCCGCGGTTCAGGAATCGTCACCCGGCGGCCTCTCATT
  CTGCAGCTCATCTTCTCAAAAACAGAACATGCCGAGTTTTTGCACTGCAAGTCCAAAAAGTTTACAGACT
  TTGATGAAGTCCGGCAGGAGATTGAAGCAGAGACCGACAGGGTCACGGGGACCAACAAAGGCATCTCCCC
  AGTGCCCATCAACCTTCGAGTCTACTCGCCACACGTGTTGAACTTGACCCTCATCGACCTCCCGGGTATC
  ACCAAGGTGCCTGTGGGCGACCAGCCTCCAGACATCGAGTACCAGATCAAGGACATGATCCTGCAGTTCA
  TCAGCCGGGAGAGCAGCCTCATTCTGGCTGTCACGCCCGCCAACATGGACCTGGCCAACTCCGACGCCCT
  CAAGCTGGCCAAGGAAGTCGATCCCCAAGGCCTACGGACCATCGGTGTCATCACCAAGCTTGACCTGATG
  GACGAGGGCACCGACGCCAGGGACGTCTTGGAGAACAAGTTGCTCCCGTTGAGAAGAGGCTACATTGGCG
  TGGTGAACCGCAGCCAGAAGGATATTGAGGGCAAGAAGGACATCCGTGCAGCACTGGCAGCTGAGAGGAA
  GTTCTTCCTCTCCCACCCGGCCTACCGGCACATGGCCGACCGCATGGGCACGCCACATCTGCAGAAGACG
  CTGAATCAGCAACTGACCAACCACATCCGGGAGTCGCTGCCGGCCCTACGTAGCAAACTACAGAGCCAGC
  TGCTGTCCCTGGAGAAGGAGGTGGAGGAGTACAAGAACTTTCGGCCCGACGACCCCACCCGCAAAACCAA
  AGCCCTGCTGCAGATGGTCCAGCAGTTTGGGGTGGATTTTGAGAAGAGGATCGAGGGCTCAGGAGATCAG
  GTGGACACTCTGGAGCTCTCCGGGGGCGCCCGAATCAATCGCATCTTCCACGAGCGGTTCCCATTTGAGC
  TGGTGAAGATGGAGTTTGACGAGAAGGACTTACGACGGGAGATCAGCTATGCCATTAAGAACATCCATGG
  AGTCAGGACCGGGCTTTTCACCCCGGACTTGGCATTCGAGGCCATTGTGAAAAAGCAGGTCGTCAAGCTG
  AAAGAGCCCTGTCTGAAATGTGTCGACCTGGTTATCCAGGAGCTAATCAATACAGTTAGGCAGTGTACCA
  GTAAGCTCAGTTCCTACCCCCGGTTGCGAGAGGAGACAGAGCGAATCGTCACCACTTACATCCGGGAACG
  GGAGGGGAGAACGAAGGACCAGATTCTTCTGCTGATCGACATTGAGCAGTCCTACATCAACACGAACCAT
  GAGGACTTCATCGGGTTTGCCAATGCCCAGCAGAGGAGCACGCAGCTGAACAAGAAGAGAGCCATCCCCA
  ATCAGGTGATCCGCAGGGGCTGGCTGACCATCAACAACATCAGCCTGATGAAAGGCGGCTCCAAGGAGTA
  CTGGTTTGTGCTGACTGCCGAGTCACTGTCCTGGTACAAGGATGAGGAGGAGAAAGAGAAGAAGTACATG
  CTGCCTCTGGACAACCTCAAGATCCGTGATGTGGAGAAGGGCTTCATGTCCAACAAGCACGTCTTCGCCA
  TCTTCAACACGGAGCAGAGAAACGTCTACAAGGACCTGCGGCAGATCGAGCTGGCCTGTGACTCCCAGGA
  AGACGTGGACAGCTGGAAGGCCTCGTTCCTCCGAGCTGGCGTCTACCCCGAGAAGGACCAGGCAGAAAAC
  GAGGATGGGGCCCAGGAGAACACCTTCTCCATGGACCCCCAACTGGAGCGGCAGGTGGAGACCATTCGCA
  ACCTGGTGGACTCATACGTGGCCATCATCAACAAGTCCATCCGCGACCTCATGCCAAAGACCATCATGCA
  CCTCATGATCAACAATACGAAGGCCTTCATCCACCACGAGCTGCTGGCCTACCTATACTCCTCGGCAGAC
  CAGAGCAGCCTCATGGAGGAGTCGGCTGACCAGGCACAGCGGCGGGACGACATGCTGCGCATGTACCATG
  CCCTCAAGGAGGCGCTCAACATCATCGGTGACATCAGCACCAGCACTGTGTCCACGCCTGTACCCCCGCC
  TGTCGATGACACCTGGCTCCAGAGCGCCAGCAGCCACAGCCCCACTCCACAGCGCCGACCGGTGTCCAGC
  ATACACCCCCCTGGCCGGCCCCCAGCAGTGAGGGGCCCCACTCCAGGGCCCCCCCTGATTCCTGTTCCCG
  TGGGGGCAGCAGCCTCCTTCTCGGCGCCCCCAATCCCATCCCGGCCTGGACCCCAGAGCGTGTTTGCCAA
  CAGTGACCTCTTCCCAGCCCCGCCTCAGATCCCATCTCGGCCAGTTCGGATCCCCCCAGGGATTCCCCCA
  GGAGTGCCCAGCAGAAGACCCCCTGCTGCGCCCAGCCGGCCCACCATTATCCGCCCAGCCGAGCCATCCC
  TGCTCGACTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004945 unedited
  GGGTCAACATTTGTATACGACTCACTATAGGGCGGCCGCGATTCGGCCGAGGCCGAGCCG
  GGAGCGGGCGTCTTGCCGAGGCCCGGGCGGGCGGGGAGCAACGGCTACAGACGCCGCGGG
  GCCAGGTCGTTGAGGGTCGGCGGCGGGCGAGGAGCGCAGGGCGCTCGGGCCGGGGGCCGC
  CGGCGCCATGGGCAACCGCGGGATGGAAGAGCTGATCCCGCTGGTCAACAAACTGCAGGA
  CGCCTTCAGCTCCATCGGCCAGAGCTGCCACCTGGACCTGCCGCAGATCGCTGTAGTGGG
  CGGCCAGAGCGCCGGCAAGAGCTCGGTGCTGGAGAACTTCGTGGGCCGGGACTTCCTTCC
  CCGCGGTTCAGGAATCGTCACCCGGCGGCCTCTCATTCTGCAGCTCATCTTCTCAAAAAC
  AGAACATGCCGAGTTTTTGCACTGCAAGTCCAAAAAGTTTACAGACTTTGATGAAGTCCG
  GCAGGAGATTGAAGCAGAGACCGACAGGGTCACGGGGACCAACAAAGGCATCTCCCCAGT
  GCCCATCAACCTTCGAGTCTACTCGCCACACGTGTTGAACTTGACCCTCATCGACCTCCC
  GGGTATCACCAAGGTGCCTGTGGGCGACCAGCCTCCAGACATCGAGTACCAGATCAAGGA
  CATGATCCTGCAGTTCATCAGCCGGGAGAGCAGCCTCATTCTGGCTGTCACGCCCGCCAA
  CATGGACCTGGCCAACTCCGACGCCCTCAAGCTGGCCAAGGAAAGTCGATCCCCCAGGCC
  TACGGACCATTCGGTGTCATCACCAAGCTTGACCTGATGGACGAGGGCACCGACGCCAGG
  GACGTCCTGGAGAACAGTTGCTCCCGTTGAGAAGAGCTACATTGGCGGTGGTGACCGCAG
  CCAGAAGATATGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004945 unedited
  NTTCNTTGNCCGCGGCCGCAATCTAGGATCGAGNTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTAACCAGCAGGGTCTTCCTTTTATTTTAGTTTATTAATAAAATACAGAGGGCAG
  GCACTTACAGGGGTCAAACTGACCGAGGAGTGGGTGAGGTCTCCTAAAAAAGAGGCCGCC
  CTGGGCCCACCCATCAGGGAGGCATGGGCGGAAGCTGAAAGGCCCCCAAAACCCCCCGCC
  ACCACCACCCACATAGCCCAAGCCCAGCCACCCTGGGGGACCCAGGAGGAGGAGGAGGAA
  AGGAATAGGGCAAGGCCGGCCCGGGCCAACGGTTCCGGGCCTAGCAAAGGCACCTTTGGC
  AGCCGAGGCTGGGCCAGGCTCAGGCTGTGTGGTTAATATGGCCGGAAAGGACTATGTACA
  CCTCAGGCCTGCCAGCTGGGCACTACCGAGCCCACTGGGCCAGGCTTGCCGTTGGGGAGC
  TCCTGCTAAGGGGAAGTTGATATACCCCTGCACCCCAGCAAGGCCTGGGGATGTAGGCGC
  CCTTGGTGCCCGCACTTATAGAAGGCCTGCCCTACATGGGCTTTCCCAGCCCAAGAAGAC
  CTTGGTAGAGTCCCCCCCAGCCTGGACCCCTCATTCAGGATGGTGTCAAGGGTTCTGGGT
  CCCCCCTCTGCACCCTGAGACTCCATCCCCCAAAGTGCAACACCCCAGCGCCCCCTGCCT
  GGAGCTCCAGGGCCCCTTTGCGCAGGGCGGGGTCCAGCCAGGCACAGGGGCCGGCCCTGA
  ACGGGGGCAGCGTAAGGAAAAGCCAGGGCTGCCCCTGAAAGCCTGTCCCACATAGGGCGG
  GGGGAGGCCCCAAACACTGAACTCCTGCCCCGGGTTCCGTGAGGCCCCCCGAAGCAGCCC
  CCTTGAGGCCAATCAACCAGGATGGCTCGTTGCCGTAAATGGGGCCC
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_004945
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004945.2, NP_004936.2
• RefSeq Size: 3621 bp
• RefSeq ORF: 2601 bp
• Locus ID: 1785
• Cytogenetics: 19p13.2
• Domains: dynamin_2, dynamin, PH, GED
• Protein Families: Transcription Factors
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis
• Gene Summary: 'Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Five alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1. The resulting protein (isoform 3) is shorter, compared to isoform 1.