Dynamin 2 (DNM2) (NM_004945) Human Untagged Clone

CAT#: SC324242

DNM2 (untagged)-Human dynamin 2 (DNM2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNM2
• Synonyms: CMT2M; CMTDI1; CMTDIB; DI-CMTB; DYN2; DYNII; LCCS5
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004945.2 GCGGCGACCGTGAGGCCGAGCCGGGAGCGGGCGTCTTGCCGAGGCCCGGGCGGGCGGGGA
  GCAACGGCTACAGACGCCGCGGGGCCAGGTCGTTGAGGGTCGGCGGCGGGCGAGGAGCGC
  AGGGCGCTCGGGCCGGGGGCCGCCGGCGCCATGGGCAACCGCGGGATGGAAGAGCTGATC
  CCGCTGGTCAACAAACTGCAGGACGCCTTCAGCTCCATCGGCCAGAGCTGCCACCTGGAC
  CTGCCGCAGATCGCTGTAGTGGGCGGCCAGAGCGCCGGCAAGAGCTCGGTGCTGGAGAAC
  TTCGTGGGCCGGGACTTCCTTCCCCGCGGTTCAGGAATCGTCACCCGGCGGCCTCTCATT
  CTGCAGCTCATCTTCTCAAAAACAGAACATGCCGAGTTTTTGCACTGCAAGTCCAAAAAG
  TTTACAGACTTTGATGAAGTCCGGCAGGAGATTGAAGCAGAGACCGACAGGGTCACGGGG
  ACCAACAAAGGCATCTCCCCAGTGCCCATCAACCTTCGAGTCTACTCGCCACACGTGTTG
  AACTTGACCCTCATCGACCTCCCGGGTATCACCAAGGTGCCTGTGGGCGACCAGCCTCCA
  GACATCGAGTACCAGATCAAGGACATGATCCTGCAGTTCATCAGCCGGGAGAGCAGCCTC
  ATTCTGGCTGTCACGCCCGCCAACATGGACCTGGCCAACTCCGACGCCCTCAAGCTGGCC
  AAGGAAGTCGATCCCCAAGGCCTACGGACCATCGGTGTCATCACCAAGCTTGACCTGATG
  GACGAGGGCACCGACGCCAGGGACGTCTTGGAGAACAAGTTGCTCCCGTTGAGAAGAGGC
  TACATTGGCGTGGTGAACCGCAGCCAGAAGGATATTGAGGGCAAGAAGGACATCCGTGCA
  GCACTGGCAGCTGAGAGGAAGTTCTTCCTCTCCCACCCGGCCTACCGGCACATGGCCGAC
  CGCATGGGCACGCCACATCTGCAGAAGACGCTGAATCAGCAACTGACCAACCACATCCGG
  GAGTCGCTGCCGGCCCTACGTAGCAAACTACAGAGCCAGCTGCTGTCCCTGGAGAAGGAG
  GTGGAGGAGTACAAGAACTTTCGGCCCGACGACCCCACCCGCAAAACCAAAGCCCTGCTG
  CAGATGGTCCAGCAGTTTGGGGTGGATTTTGAGAAGAGGATCGAGGGCTCAGGAGATCAG
  GTGGACACTCTGGAGCTCTCCGGGGGCGCCCGAATCAATCGCATCTTCCACGAGCGGTTC
  CCATTTGAGCTGGTGAAGATGGAGTTTGACGAGAAGGACTTACGACGGGAGATCAGCTAT
  GCCATTAAGAACATCCATGGAGTCAGGACCGGGCTTTTCACCCCGGACTTGGCATTCGAG
  GCCATTGTGAAAAAGCAGGTCGTCAAGCTGAAAGAGCCCTGTCTGAAATGTGTCGACCTG
  GTTATCCAGGAGCTAATCAATACAGTTAGGCAGTGTACCAGTAAGCTCAGTTCCTACCCC
  CGGTTGCGAGAGGAGACAGAGCGAATCGTCACCACTTACATCCGGGAACGGGAGGGGAGA
  ACGAAGGACCAGATTCTTCTGCTGATCGACATTGAGCAGTCCTACATCAACACGAACCAT
  GAGGACTTCATCGGGTTTGCCAATGCCCAGCAGAGGAGCACGCAGCTGAACAAGAAGAGA
  GCCATCCCCAATCAGGTGATCCGCAGGGGCTGGCTGACCATCAACAACATCAGCCTGATG
  AAAGGCGGCTCCAAGGAGTACTGGTTTGTGCTGACTGCCGAGTCACTGTCCTGGTACAAG
  GATGAGGAGGAGAAAGAGAAGAAGTACATGCTGCCTCTGGACAACCTCAAGATCCGTGAT
  GTGGAGAAGGGCTTCATGTCCAACAAGCACGTCTTCGCCATCTTCAACACGGAGCAGAGA
  AACGTCTACAAGGACCTGCGGCAGATCGAGCTGGCCTGTGACTCCCAGGAAGACGTGGAC
  AGCTGGAAGGCCTCGTTCCTCCGAGCTGGCGTCTACCCCGAGAAGGACCAGGCAGAAAAC
  GAGGATGGGGCCCAGGAGAACACCTTCTCCATGGACCCCCAACTGGAGCGGCAGGTGGAG
  ACCATTCGCAACCTGGTGGACTCATACGTGGCCATCATCAACAAGTCCATCCGCGACCTC
  ATGCCAAAGACCATCATGCACCTCATGATCAACAATACGAAGGCCTTCATCCACCACGAG
  CTGCTGGCCTACCTATACTCCTCGGCAGACCAGAGCAGCCTCATGGAGGAGTCGGCTGAC
  CAGGCACAGCGGCGGGACGACATGCTGCGCATGTACCATGCCCTCAAGGAGGCGCTCAAC
  ATCATCGGTGACATCAGCACCAGCACTGTGTCCACGCCTGTACCCCCGCCTGTCGATGAC
  ACCTGGCTCCAGAGCGCCAGCAGCCACAGCCCCACTCCACAGCGCCGACCGGTGTCCAGC
  ATACACCCCCCTGGCCGGCCCCCAGCAGTGAGGGGCCCCACTCCAGGGCCCCCCCTGATT
  CCTGTTCCCGTGGGGGCAGCAGCCTCCTTCTCGGCGCCCCCAATCCCATCCCGGCCTGGA
  CCCCAGAGCGTGTTTGCCAACAGTGACCTCTTCCCAGCCCCGCCTCAGATCCCATCTCGG
  CCAGTTCGGATCCCCCCAGGGATTCCCCCAGGAGTGCCCAGCAGAAGACCCCCTGCTGCG
  CCCAGCCGGCCCACCATTATCCGCCCAGCCGAGCCATCCCTGCTCGACTAGGCCTCGAGG
  GGGGCGTGCTCTCGGGGGGGCCTCACGCACCCGCGGCGCAGGAGCTTCAGTGGTCTGGGG
  CCCTCCGCCGCCCCTATGCTGGGACCAGGCTCCCAGTGGGCAGCCCTGGCCTCTTCCTTA
  ACGCTGGCCCCGGTCCAGGGCCGGCCCCTGTGCCTGGCTGGACACCGCACTGCGCAAAGG
  GGCCCTGGAGCTCCAGGCAGGGGGCGCTGGGGTGTTGCACTTTGGGGGATGGAGTCTCAG
  GGTGGCAGAGGGGGGACCAGAACCCTTGACACCATCCTGAATGAGGGGTCCAGCCTGGGG
  GGGACTCTACCAAGGTCTTCTTGGGCTGGGAAAGCCCATGTAGGGCAGGCCTTCTATAAG
  TGCGGGCACCAAGGGCGCCTACATCCCCAGGCCTTGCTGGGGTGCAGGGGTATATCAACT
  TCCCATTAGCAGGAGCTCCCCAGCGGCAAGCCTGGCCCAGTGGGCTCGGTAGTGCCCAGC
  TGGCAGGCCTGAGGTGTACATAGTCCTTCCCGGCCATATTAACCACACAGCCTGAGCCTG
  GCCCAGCCTCGGCTGCCAGAGGTGCCTTTGCTAGGCCCGGAGCCGTTGGCCCGGGCCGGC
  CTTGCCCTATTCCTCTCCTCCTCCTCCTCCTGGGTCCCCCAGGGTGGCTGGGCTTGGGCT
  ATGTGGGTGGTGGTGGCGGGGGGTCTTGGGGGCCTCTCAGCTCCCGCCCATGCCTCCCTG
  ATGGGTGGGCCCAGGGCGGCCTCTCTCTGAGGAGACCTCACCCACTCCTCGCTCAGTTTG
  ACCACTGTAAGTGCCTGCACTCTGTATTCTATTAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_004945
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004945.2, NP_004936.2
• RefSeq Size: 3621 bp
• RefSeq ORF: 2601 bp
• Locus ID: 1785
• Cytogenetics: 19p13.2
• Domains: dynamin_2, dynamin, PH, GED
• Protein Families: Transcription Factors
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis
• Gene Summary: 'Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Five alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1. The resulting protein (isoform 3) is shorter, compared to isoform 1.