Kinesin Heavy Chain 2 (KIF2A) (NM_004520) Human Untagged Clone

CAT#: SC117315

KIF2A (untagged)-Human kinesin heavy chain member 2A (KIF2A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KIF2A
• Synonyms: CDCBM3; HK2; KIF2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004520 edited
  GAATTCGGCACGAGGTCCCTCGGCCCGCTGCTGCTGCTCCAGATGAGGTGATGGCAACGG
  CCAACTTCGGCAAGATCCAGATCGGGATTTACGTGGAGATCAAGCGCAGCGATGGCCGAA
  TACATCAAGCAATGGTAACATCTTTAAATGAAGATAATGAAAGTGTAACTGTTGAATGGA
  TAGAAAATGGAGATACAAAAGGCAAAGAGATTGACCTGGAGAGCATCTTTTCACTTAACC
  CTGACCTTGTTCCTGATGAAGAAATTGAACCCAGTCCAGAAACACCTCCACCTCCAGCAT
  CCTCAGCCAAAGTAAACAAAATTGTAAAGAATCGACGGACTGTAGCTTCTATTAAGAATG
  ACCCTCCTTCAAGAGATAATAGAGTGGTTGGTTCAGCACGTGCACGGCCCAGTCAATTTC
  CTGAACAGTCTTCCTCTGCACAACAGAATGGTAGTGTTTCAGATATATCTCCAGTTCAAG
  CTGCAAAAAAGGAATTTGGACCCCCTTCACGTAGAAAATCTAATTGTGTGAAAGAAGTAG
  AAAAACTGCAAGAAAAACGAGAGAAAAGGAGATTGCAACAGCAAGAACTTAGAGAAAAAA
  GAGCCCAGGACGTTGATGCTACAAACCCAAATTATGAAATTATGTGTATGATCAGAGACT
  TTAGAGGAAGTTTGGATTATAGACCATTAACAACAGCAGATCCTATTGATGAACATAGGA
  TATGTGTGTGTGTAAGAAAACGACCACTCAATAAAAAAGAAACTCAAATGAAAGATCTTG
  ATGTAATCACAATTCCTAGTAAAGATGTTGTGATGGTACATGAACCAAAACAAAAAGTAG
  ATTTAACAAGGTACCTAGAAAACCAAACATTTCGTTTTGATTATGCCTTTGATGACTCAG
  CTCCTAATGAAATGGTTTACAGGTTTACTGCTAGACCACTAGTGGAAACTATATTTGAAA
  GGGGAATGGCTACATGCTTTGCTTATGGGCAGACTGGAAGTGGAAAAACTCATACTATGG
  GTGGTGACTTTTCAGGAAAGAACCAAGATTGTTCTAAAGGAATTTATGCATTAGCAGCTC
  GAGATGTCTTTTTAATGCTAAAGAAGCCAAACTATAAGAAGCTAGAACTTCAAGTATATG
  CAACCTTCTTTGAAATTTATAGTGGAAAGGTGTTTGACTTGCTAAACAGGAAAACAAAAT
  TAAGAGTTCTAGAAGATGGAAAACAGCAGGTTCAAGTGGTGGGATTACAGGAACGGGAGG
  TCAAATGTGTTGAAGATGTACTGAAACTCATTGACATAGGCAACAGTTGCAGAACATCCG
  GTCAAACATCTGCAAATGCACATTCATCTCGGAGCCATGCAGTGTTTCAGATTATTCTTA
  GAAGGAAAGGAAAACTACATGGCAAATTTTCTCTCATTGATTTGGCTGGAAATGAAAGAG
  GAGCTGATACTTCCAGTGCGGACAGGCAAACTAGGCTTGAAGGTGCTGAAATTAATAAAA
  GCCTTTTAGCACTCAAGGAGTGCATCAGAGCCTTAGGTAGAAATAAACCTCATACTCCTT
  TCCGTGCAAGTAAACTCACTCAGGTGTTAAGAGATTCTTTCATAGGTGAAAACTCTCGTA
  CCTGCATGATTGCCACAATCTCTCCAGGAATGGCATCCTGTGAAAATACTCTTAATACAT
  TAAGATATGCAAATAGAGTAAAGGAGTTTGGAATTAGTCCATCAGACATTCCCTTCTCAC
  AGGGTAGTGGCAGTCGCCCTGATCTCTCTCCTTCTTATGAATATGACGACTTTTCTCCTT
  CAGTTACCAGGGTCAAAGAATTGACTGTAGATCCAACTGCTGCTGGTGATGTTCGTCCAA
  TAATGCACCATCCACCAAACCAGATTGATGACTTAGAGACACAGTGGGGTGTGGGGAGTT
  CCCCTCAGAGAGATGATCTAAAACTTCTTTGTGAACAAAATGAAGAAGAAGTCTCTCCAC
  AGTTGTTTACTTTCCACGAAGCTGTTTCACAAATGGTAGAAATGGAAGAACAAGTTGTAG
  AAGATCACAGGGCAGTGTTCCAGGAATCTATTCGGTGGTTAGAAGATGAAAAGGCCCTCT
  TAGAGATGACTGAAGAAGTAGATTATGATGTCGATTCATATGCTACACAACTTGAAGCTA
  TTCTTGAGCAAAAAATAGACATTTTAACTGAACTGCGGGATAAAGTGAAATCTTTCCGTG
  CAGCTCTACAAGAGGAGGAACAAGCCAGCAAGCAAATCAACCCGAAGAGACCCCGTGCCC
  TTTAAACCGGCATTTGCTGCTAAAGGATACCCAGAACCCTCACTACTGTAACATACAACG
  GTTCAGCTGTAAGGGCCATTTGAAAGTTTGGAATTTTAAGTGTCTGTGGAAAATGTTTTG
  TCCTTCACCTGAATTACATTTCAATTTTGTGAAACACTCTTTTGTCTACAAAATGCTTCT
  AGTCCAGGAGGCACAACCAAGAACTGGGATTAATGAAGCATTTTGTTTCATTTACACAAA
  TAGTGATTTACTTTTGGAGATCCTTGTCAGTTTTATTTTCTATTTGATGAAGTAAGACTG
  TGGACTCAATCCAGAGCCAGATAGTAGGGGGAAGCCACAGCATTTCCTTTTAACTCAGTT
  CAATTTTTGTAGTGAGACTGAGCAGTTTTAAATCCTTTGCGTGCATGCATACCTCATCAG
  TGATTGTACATACCTTGCCCACTCCTAGAGACAGCTGTGCTCACCTTTTCCTGCTTTGTG
  CCTTGATTAAGGCTACTGACCCTAAATTTCTGAAGCACAGCCAAGAAAAATTACATTCCT
  TGTCATTGTAAATTACCTTTGTGTGTACATTTTTACTGTATTTGAGACATTTTTTGTGTG
  TGACTAGTTAATTTTGCAGGATGTGCCATATCATTGAACGGAACTAAAGTCTGTGACAGT
  GGATATAGCTGCTGGACCATTCCATCTTATATGTAAAGAAATCTGGAATTATTATTTTAA
  AACCATATAACATGTGATTATAATTTTTCTTAGCATTTTCTTTGTAAAGAACTACAATAT
  AAACTAGTTGGTGTATAATAAAAAGTAATGAAATTCAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004520 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTCCCTCGGCCCGCTGCTGC
  TGCTCCAGATGAGGTGATGGCAACGGCCAACTTCGGCAAGATCCAGATCGGGATTTACGT
  GGAGATCAAGCGCAGCGATGGCCGAATACATCAAGCAATGGTAACATCTTTAAATGAAGA
  TAATGAAAGTGTAACTGTTGAATGGATAGAAAATGGAGATACAAAAGGCAAAGAGATTGA
  CCTGGAGAGCATCTTTTCACTTAACCCTGACCTTGTTCCTGATGAAGAAATTGAACCCAG
  TCCAGAAACACCTCCACCTCCAGCATCCTCAGCCAAAGTAAACAAAATTGTAAAGAATCG
  ACGGACTGTAGCTTCTATTAAGAATGACCCTCCTTCAAGAGATAATAGAGTGGTTGGTTC
  AGCACGTGCACGGCCCAGTCAATTTCCTGAACAGTCTTCCTCTGCACAACAGAATGGTAG
  TGTTTCAGATATATCTCCAGTTCAAGCTGCAAAAAAGGAATTTGGACCCCCTTCACGTAA
  CTAAATCTAATTGTGTGAAAGAAGTAGAAAAACTGCAAGAAAAACGAGAGAAAAGGAGAA
  TTGCACAGCAAGAACTTAGAGAANAAAGAGCCCAGGACGTTGATGCTACANACCCAAATT
  ATGAAATTATGTGTATGATCAGAGACTNTAGAGGAAAGTTTGATTATAGACCATTNACCA
  CAGCAGATCCTATTGATGAACATANGATATGTGNTGTGTGTAAGAAAACCGACCCACTCA
  TANNAAAGAAACTCAAATGAAAAGATCTTGATGTAATCACAATTCCTAGTAAAGATGTNG
  NGATGGTCCATGAACCCAAACAAAAGTAGATTTACAGGTACCTANAAAACCACCATTTCG
  TTTGATTATGCTTTGAGACTCACTCCTATGAATGGNTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004520 unedited
  TTGGACCGCGNGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAATTTCATTAC
  TTTTTATTATACACCAACTAGTTTATATTGTAGTTCTTTACAAAGAAAATGCTAAGAAAA
  ATTATAATCACATGTTATATGGTTTTAAAATAATAATTCCAGATTTCTTTACATATAAGA
  TGGAATGGTCCAGCAGCTATATCCACTGTCACAGACTTTAGTTCCGTTCAATGATATGGC
  ACATCCTGCAAAATTAACTAGTCACACACAAAAAATGTCTCAAATACAGTAAAAATGTAC
  ACACAAAGGTAATTTACAATGACAAGGAATGTAATTTTTCTTGGCTGTGCTTCAGAAATT
  TAGGGTCAGTAGCCTTAATCAAGGCACAAAGCAGGAAAAGGTGAGCACAGCTGTCTCTAG
  GAGTGGGCAAGGTATGTACAATCACTGATGAGGTATGCATGCACGCAAAGGATTTAAAAC
  TGCTCAGTCTCACTACAAAAATTGAACTGAGTTAAAAGGAAATGCTGTGGCTTCCCCCTA
  CTATCTGGCTCTGGATTGAGTCCACAGTCTTACTTCATCAAATAGAAAATAAAACTGACA
  AGGATCTCCAAAAGTAAATCACTATTTGTGTAAATGAAACAAAATGCTTCATTAATCCCA
  GTTCTTGGTTGTGCCTCCTGGACTAGAAGCATTTTGTAGACAAAAGAGTGTTTCACAAAA
  TTGAAATGTAATTCAGGTGAAGGACAAAACATTTTCCACAGACACTTAAAATTCCAAACT
  TTCAAATGGNCCCTTACAGCTGAACCGTTGTATGTTACAGTAGTGAAGGGGTCTGGGTAT
  CCTTTAGCAGCAAATGCCGGNTTAAAGGGCACGGNNGTCTCTTNCGGNNTGNNATTGCTG
  CTGGGCTGTTCCTCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004520
• Insert Size: 3200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004520.1, NP_004511.1
• RefSeq Size: 2905 bp
• RefSeq ORF: 2040 bp
• Locus ID: 3796
• Cytogenetics: 5q12.1
• Domains: kinesin
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene is a plus end-directed motor required for normal mitotic progression. The encoded protein is required for normal spindle activity during mitosis and is necessary for normal brain development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]'
  Transcript Variant: This variant (1) lacks an alternate in-frame exon compared to variant 2. The resulting isoform (1) has the same N- and C-termini but is shorter compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.