Kinesin Heavy Chain 2 (KIF2A) (NM_004520) Human Untagged Clone

CAT#: SC318185

KIF2A (untagged)-Human kinesin heavy chain member 2A (KIF2A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KIF2A
• Synonyms: CDCBM3; HK2; KIF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004520, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAACGGCCAACTTCGGCAAGATCCAGATCGGGATTTACGTGGAGATCAAGCGCAGCGATGGCCGAA
  TACATCAAGCAATGGTAACATCTTTAAATGAAGATAATGAAAGTGTAACTGTTGAATGGATAGAAAATGG
  AGATACAAAAGGCAAAGAGATTGACCTGGAGAGCATCTTTTCACTTAACCCTGACCTTGTTCCTGATGAA
  GAAATTGAACCCAGTCCAGAAACACCTCCACCTCCAGCATCCTCAGCCAAAGTAAACAAAATTGTAAAGA
  ATCGACGGACTGTAGCTTCTATTAAGAATGACCCTCCTTCAAGAGATAATAGAGTGGTTGGTTCAGCACG
  TGCACGGCCCAGTCAATTTCCTGAACAGTCTTCCTCTGCACAACAGAATGGTAGTGTTTCAGATATATCT
  CCAGTTCAAGCTGCAAAAAAGGAATTTGGACCCCCTTCACGTAGAAAATCTAATTGTGTGAAAGAAGTAG
  AAAAACTGCAAGAAAAACGAGAGAAAAGGAGATTGCAACAGCAAGAACTTAGAGAAAAAAGAGCCCAGGA
  CGTTGATGCTACAAACCCAAATTATGAAATTATGTGTATGATCAGAGACTTTAGAGGAAGTTTGGATTAT
  AGACCATTAACAACAGCAGATCCTATTGATGAACATAGGATATGTGTGTGTGTAAGAAAACGACCACTCA
  ATAAAAAAGAAACTCAAATGAAAGATCTTGATGTAATCACAATTCCTAGTAAAGATGTTGTGATGGTACA
  TGAACCAAAACAAAAAGTAGATTTAACAAGGTACCTAGAAAACCAAACATTTCGTTTTGATTATGCCTTT
  GATGACTCAGCTCCTAATGAAATGGTTTACAGGTTTACTGCTAGACCACTAGTGGAAACTATATTTGAAA
  GGGGAATGGCTACATGCTTTGCTTATGGGCAGACTGGAAGTGGAAAAACTCATACTATGGGTGGTGACTT
  TTCAGGAAAGAACCAAGATTGTTCTAAAGGAATTTATGCATTAGCAGCTCGAGATGTCTTTTTAATGCTA
  AAGAAGCCAAACTATAAGAAGCTAGAACTTCAAGTATATGCAACCTTCTTTGAAATTTATAGTGGAAAGG
  TGTTTGACTTGCTAAACAGGAAAACAAAATTAAGAGTTCTAGAAGATGGAAAACAGCAGGTTCAAGTGGT
  GGGATTACAGGAACGGGAGGTCAAATGTGTTGAAGATGTACTGAAACTCATTGACATAGGCAACAGTTGC
  AGAACATCCGGTCAAACATCTGCAAATGCACATTCATCTCGGAGCCATGCAGTGTTTCAGATTATTCTTA
  GAAGGAAAGGAAAACTACATGGCAAATTTTCTCTCATTGATTTGGCTGGAAATGAAAGAGGAGCTGATAC
  TTCCAGTGCGGACAGGCAAACTAGGCTTGAAGGTGCTGAAATTAATAAAAGCCTTTTAGCACTCAAGGAG
  TGCATCAGAGCCTTAGGTAGAAATAAACCTCATACTCCTTTCCGTGCAAGTAAACTCACTCAGGTGTTAA
  GAGATTCTTTCATAGGTGAAAACTCTCGTACCTGCATGATTGCCACAATCTCTCCAGGAATGGCATCCTG
  TGAAAATACTCTTAATACATTAAGATATGCAAATAGGGTCAAAGAATTGACTGTAGATCCAACTGCTGCT
  GGTGATGTTCGTCCAATAATGCACCATCCACCAAACCAGATTGATGACTTAGAGACACAGTGGGGTGTGG
  GGAGTTCCCCTCAGAGAGATGATCTAAAACTTCTTTGTGAACAAAATGAAGAAGAAGTCTCTCCACAGTT
  GTTTACTTTCCACGAAGCTGTTTCACAAATGGTAGAAATGGAAGAACAAGTTGTAGAAGATCACAGGGCA
  GTGTTCCAGGAATCTATTCGGTGGTTAGAAGATGAAAAGGCCCTCTTAGAGATGACTGAAGAAGTAGATT
  ATGATGTCGATTCATATGCTACACAACTTGAAGCTATTCTTGAGCAAAAAATAGACATTTTAACTGAACT
  GCGGGATAAAGTGAAATCTTTCCGTGCAGCTCTACAAGAGGAGGAACAAGCCAGCAAGCAAATCAACCCG
  AAGAGACCCCGTGCCCTTTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_004520
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004520.4, NP_004511.2
• RefSeq Size: 4058 bp
• RefSeq ORF: 2121 bp
• Locus ID: 3796
• Cytogenetics: 5q12.1
• Domains: kinesin
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene is a plus end-directed motor required for normal mitotic progression. The encoded protein is required for normal spindle activity during mitosis and is necessary for normal brain development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]'
  Transcript Variant: This variant (1) lacks an alternate in-frame exon compared to variant 2. The resulting isoform (1) has the same N- and C-termini but is shorter compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.