Glutamyl Prolyl tRNA synthetase (EPRS) (NM_004446) Human Untagged Clone

CAT#: SC117359

EPRS (untagged)-Human glutamyl-prolyl-tRNA synthetase (EPRS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EPRS
• Synonyms: EARS; GLUPRORS; HLD15; PARS; PIG32; QARS; QPRS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004446, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGACGCTCTCTCTGACCGTGAATTCAGGAGACCCTCCGCTAGGAGCTTTGCTGGCAGTAGAACACG
  TGAAAGACGATGTCAGCATTTCCGTTGAAGAAGGGAAAGAGAATATTCTTCATGTTTCTGAAAATGTGAT
  ATTCACAGATGTGAATTCTATACTTCGCTACTTGGCTAGAGTTGCAACTACAGCTGGGTTATATGGCTCT
  AATCTGATGGAACATACTGAGATTGATCACTGGTTGGAGTTCAGTGCTACAAAATTATCTTCATGTGATT
  CCTTTACTTCTACAATTAATGAACTCAATCATTGCCTGTCTCTGAGAACATACTTAGTTGGAAACTCCTT
  GAGTTTAGCAGATTTATGTGTTTGGGCCACCCTAAAAGGAAATGCTGCCTGGCAAGAACAGTTGAAACAG
  AAGAAAGCTCCAGTTCATGTAAAACGTTGGTTTGGCTTTCTTGAAGCCCAGCAGGCCTTCCAGTCAGTAG
  GTACCAAGTGGGATGTTTCAACAACCAAAGCTCGAGTGGCACCTGAGAAAAAGCAAGATGTTGGGAAATT
  TGTTGAGCTTCCAGGTGCGGAGATGGGAAAGGTTACCGTCAGATTTCCTCCAGAGGCCAGTGGTTACTTA
  CACATTGGGCATGCAAAAGCTGCTCTTCTGAACCAGCACTACCAGGTTAACTTTAAAGGGAAACTGATCA
  TGAGATTTGATGACACAAATCCTGAAAAAGAAAAGGAAGATTTTGAGAAGGTTATCTTGGAAGATGTTGC
  AATGTTGCATATCAAACCAGATCAATTTACTTATACTTCGGATCATTTTGAAACTATAATGAAGTATGCA
  GAGAAGCTAATTCAAGAAGGGAAGGCTTATGTGGATGATACTCCTGCTGAACAGATGAAAGCAGAACGTG
  AGCAGAGGATAGACTCTAAACATAGAAAAAACCCTATTGAGAAGAATCTACAAATGTGGGAAGAAATGAA
  AAAAGGGAGCCAGTTTGGTCAGTCCTGTTGTTTGCGAGCAAAAATTGACATGAGTAGTAACAATGGATGC
  ATGAGAGATCCAACCCTTTATCGCTGCAAAATTCAACCACATCCAAGAACTGGAAATAAATACAATGTTT
  ATCCAACATATGATTTTGCCTGCCCCATAGTTGACAGCATCGAAGGTGTTACACATGCCCTGAGAACAAC
  AGAATACCATGACAGAGATGAGCAGTTTTACTGGATTATTGAAGCTTTAGGCATAAGAAAACCATATATT
  TGGGAATATAGTCGGCTAAATCTCAACAACACAGTGCTATCCAAAAGAAAACTCACATGGTTTGTCAATG
  AAGGACTAGTAGATGGATGGGATGACCCAAGATTTCCTACGGTTCGTGGTGTACTGAGAAGAGGGATGAC
  AGTTGAAGGACTGAAACAGTTTATTGCTGCTCAGGGCTCCTCACGTTCAGTCGTGAACATGGAGTGGGAC
  AAAATCTGGGCGTTTAACAAAAAGGTTATTGACCCAGTGGCTCCACGATATGTTGCATTACTGAAGAAAG
  AAGTGATCCCAGTGAATGTACCTGAAGCTCAGGAGGAGATGAAAGAAGTAGCCAAACACCCAAAGAATCC
  TGAGGTTGGCTTGAAGCCTGTGTGGTATAGTCCCAAAGTTTTCATTGAAGGTGCTGATGCAGAGACTTTT
  TCGGAGGGTGAGATGGTTACATTTATAAATTGGGGCAACCTCAACATTACAAAAATACACAAAAATGCAG
  ATGGAAAAATCATATCTCTTGATGCAAAGTTGAATTTGGAAAACAAAGACTACAAGAAAACCACTAAGGT
  CACTTGGCTTGCAGAGACTACACATGCTCTTCCTATTCCAGTAATCTGTGTCACTTATGAGCACTTGATC
  ACAAAGCCAGTGCTAGGAAAAGACGAGGACTTTAAGCAGTATGTCAACAAGAACAGTAAGCATGAAGAGC
  TAATGCTAGGGGATCCCTGCCTTAAGGATTTGAAAAAAGGAGATATTATACAACTCCAGAGAAGAGGATT
  CTTCATATGTGATCAACCTTATGAACCTGTTAGCCCATATAGTTGCAAGGAAGCCCCGTGTGTTTTGATA
  TACATTCCTGATGGGCACACAAAGGAAATGCCAACATCAGGGTCAAAGGAAAAGACCAAAGTAGAAGCCA
  CAAAAAATGAGACCTCTGCTCCTTTTAAGGAAAGACCAACACCTTCTCTGAATAATAATTGTACTACATC
  TGAGGATTCCTTGGTCCTTTACAATAGAGTGGCTGTTCAAGGAGATGTGGTTCGTGAATTAAAAGCCAAG
  AAAGCACCAAAGGAAGATGTAGATGCAGCTGTAAAACAGCTTTTGTCTTTGAAAGCTGAATATAAGGAGA
  AAACTGGCCAGGAATATAAACCTGGAAACCCTCCTGCTGAAATAGGACAGAATATTTCTTCTAATTCCTC
  AGCAAGTATTCTGGAAAGTAAATCTCTGTATGATGAAGTTGCTGCACAAGGGGAGGTGGTTCGTAAGCTA
  AAAGCTGAAAAATCCCCTAAGGCTAAAATAAATGAAGCTGTAGAATGCTTACTGTCCCTGAAGGCTCAGT
  ATAAAGAAAAAACTGGGAAGGAGTACATACCTGGTCAGCCCCCATTATCTCAAAGTTCGGATTCAAGCCC
  AACCAGAAATTCTGAACCTGCTGGTTTAGAAACACCAGAAGCGAAAGTACTTTTTGACAAAGTAGCTTCT
  CAAGGGGAAGTAGTTCGGAAACTTAAAACTGAAAAAGCCCCTAAGGATCAAGTAGATATAGCTGTTCAAG
  AACTCCTTCAGCTAAAGGCACAGTACAAGTCTTTGATAGGAGTAGAGTATAAGCCTGTGTCGGCCACTGG
  AGCTGAGGACAAAGATAAGAAGAAGAAAGAAAAAGAAAATAAATCTGAAAAGCAGAATAAGCCTCAGAAA
  CAAAATGATGGCCAAAGGAAAGACCCTTCTAAAAACCAAGGAGGTGGGCTCTCATCAAGTGGAGCAGGAG
  AAGGGCAGGGGCCTAAGAAACAGACCAGGTTGGGTCTTGAGGCAAAAAAAGAAGAAAATCTTGCTGATTG
  GTATTCTCAGGTCATCACAAAGTCAGAAATGATTGAATACCATGACATAAGTGGCTGTTATATTCTTCGT
  CCCTGGGCCTATGCCATTTGGGAAGCCATCAAGGACTTTTTTGATGCTGAGATCAAGAAACTTGGTGTTG
  AAAACTGCTACTTCCCCATGTTTGTGTCTCAAAGTGCATTAGAGAAAGAGAAGACTCATGTTGCTGACTT
  TGCCCCAGAGGTTGCTTGGGTTACAAGATCTGGCAAAACCGAGCTGGCAGAACCAATTGCCATTCGTCCT
  ACTAGTGAAACAGTAATGTATCCTGCATATGCAAAATGGGTACAGTCACACAGAGACCTGCCCATCAAGC
  TCAATCAGTGGTGCAATGTGGTGCGTTGGGAATTCAAGCATCCTCAGCCTTTCCTACGTACTCGTGAATT
  TCTTTGGCAGGAAGGGCACAGTGCTTTTGCTACCATGGAAGAGGCAGCGGAAGAGGTCTTGCAGATACTT
  GACTTATATGCTCAGGTATATGAAGAACTCCTGGCAATTCCTGTTGTTAAAGGAAGAAAGACGGAAAAGG
  AAAAATTTGCAGGAGGAGACTATACAACTACAATAGAAGCATTTATATCTGCTAGTGGAAGAGCTATCCA
  GGGAGGAACATCACATCATTTAGGGCAGAATTTTTCCAAAATGTTTGAAATCGTTTTTGAAGATCCAAAG
  ATACCAGGAGAGAAGCAATTTGCCTATCAAAACTCCTGGGGCCTGACAACTCGAACTATTGGTGTTATGA
  CCATGGTTCATGGGGACAACATGGGTTTAGTATTACCACCCCGTGTAGCATGTGTTCAGGTGGTGATTAT
  TCCTTGTGGCATTACCAATGCACTTTCTGAAGAAGACAAAGAAGCGCTGATTGCAAAATGCAATGATTAT
  CGAAGGCGATTACTCAGTGTTAACATCCGCGTTAGAGCTGATTTACGAGATAATTATTCTCCAGGTTGGA
  AATTCAATCACTGGGAGCTCAAGGGAGTTCCCATTAGACTTGAAGTTGGGCCACGTGATATGAAGAGCTG
  TCAGTTTGTAGCCGTCAGACGAGATACTGGAGAAAAGCTGACAGTTGCTGAAAATGAGGCAGAGACTAAA
  CTTCAAGCTATTTTGGAAGACATCCAGGTCACCCTTTTCACAAGGGCTTCTGAAGACCTTAAGACTCATA
  TGGTTGTGGCTAATACAATGGAAGACTTTCAGAAGATACTAGATTCTGGAAAGATTGTTCAGATTCCATT
  CTGTGGGGAAATTGACTGTGAGGACTGGATCAAAAAGACCACTGCCAGGGATCAAGATCTTGAACCTGGT
  GCTCCATCCATGGGAGCTAAAAGCCTTTGCATCCCCTTCAAACCACTCTGTGAACTGCAGCCTGGAGCCA
  AATGTGTCTGTGGCAAGAACCCTGCCAAGTACTACACCTTATTTGGTCGCAGCTACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004446 unedited
  NGGGTGCAAATATTTGTAATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGCGT
  AGTTCTGGCACGAGCCAGGCGTACTGACAGGTGGACCAGCGGACTGGTGGAGATGGCGAC
  GCTCTCTCTGACCGTGAATTCAGGAGACCCTCCGCTAGGAGCTTTGCTGGCAGTAGAACA
  CGTGAAAGACGATGTCAGCATTTCCGTTGAAGAAGGGAAAGAGAATATTCTTCATGTTTC
  TGAAAATGTGATATTCACAGATGTGAATTCTATACTTCGCTACTTGGCTAGAGTTGCAAC
  TACAGCTGGGTTATATGGCTCTAATCTGATGGAACATACTGAGATTGATCACTGGTTGGA
  GTTCAGTGCTACAAAATTATCTTCATGTGATTCCTTTACTTCTACAATTAATGAACTCAA
  TCATTGCCTGTCTCTGAGAACATACTTAGTTGGAAACTCCTTGAGTTTAGCAGATTTATG
  TGTTTGGGCCACCCTAAAAGGAAATGCTGCCTGGCAAGAACAGTTGAAACAGAAGAAAGC
  TCCAGTTCATGTAAAACGTTGGTTTGGCTTTCTTGAAGCCCAGCAGGCCTTCCAGTCAGT
  AGGTACCAAGTGGGATGTTTCAACAACCAAAGCTCGAGTGGCACCTGAGAAAAAGCAAGA
  TGTTGGGAAATTTGTTGAGCTTCCAGGTGCGGAGATGGGAAAGGTTACCGTCAGATTTCC
  TCCAGAGGCCAGTGGTTACTTACACATTGGGCATGCAAAAGCTGCTCTTCTGAACCACCT
  TAACCAGGTTAACTNTAAAGGGAAACTGATCATGAGATTTGATGACACAAATCCTGAANA
  AGAAAAGGAGATTTTGAGAAGTTATCTTGGNAGATGNNTGCATGTTGCATATCAAACCAG
  ATCAATTAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004446 unedited
  TAGCTATGNACCGCGCGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTAGTTC
  ATGATATTTATTACTGGAGTTGTTTACAGAAAAGTCTTTTATCCACTGTGTAACTTTAGA
  GCATAAGAAATAATCTGTCTCTGTGGTGACCTTCATCTTTAAAAACTCTTTACTCTTTTA
  AAAAATCATAAAATGGTCTGTATCTGAGAAGATACTAATATCAATGCTTTAAAAAGTGAG
  AGGAGTTGAAGAGGGGGCTTTCGTTCATCCCTCAGTAGCTGCGACCAAATAAGGTGTAGT
  ACTTGGCAGGGTTCTTGCCACAGACACATTTGGCTCCAGGCTGCAGTTCACAGAGTGGTT
  TGAAGGGGATGCAAAGGCTTTTAGCTCCCATGGATGGAGCACCAGGTTCAAGATCTTGAT
  CCCTGGCAGTGGTCTTTTTGATCCAGTCCTCACAGTCAATTTCCCCACAGAATGGAATCT
  GAACAATCTTTCCAGAATCTAGTATCTTCTGAAAGTCTTCCATTGTATTAGCCACAACCA
  TATGAGTCTTAAGGTCTTCAGAAGCCCTTGTGAAAAGGGTGACCTGGATGTCTTCCAAAA
  TAGCTTGAAGTTTAGTCTCTGCCTCATTTTCAGCAACTGTCAGCTTTTCTCCAGTATCTC
  GTCTGACGGCTACAAACTGACAGCTCTTCATATCACGTGGCCCAACTTCAAGTCTAATGG
  GAACTCCCTTGAGCTCCCAGTGATTGAATTTCCAACCTGGAGAATAATTATCTCGTAAAT
  CAGCTCTAAACGCGGATGTTACACTNGAGTATCGCCTTCGATATCATTGCATTTTGCAAT
  CAGCGCTTCTTTGTCTTCTTCAGAAAGTGCATTGGTAATGCCACAAGAATAATCACCACC
  TGACCATGCTACACGGGTGGGTATACTAAACCCTGTTGTCCCATGAACCATGTCATACAC
  CATAGNTCGAGTGTCAGCCCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004446
• Insert Size: 4600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004446.2, NP_004437.2
• RefSeq Size: 5021 bp
• RefSeq ORF: 4539 bp
• Locus ID: 2058
• Cytogenetics: 1q41
• Domains: WHEP-TRS, tRNA-synt_1c, tRNA-synt_2b, HGTP_anticodon
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Metabolic pathways, Porphyrin and chlorophyll metabolism
• Gene Summary: 'Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined. [provided by RefSeq, Jul 2008]'