Fibroblast activation protein, alpha (FAP) (NM_004460) Human Untagged Clone

CAT#: SC117372

FAP (untagged)-Human fibroblast activation protein, alpha (FAP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FAP
• Synonyms: DPPIV; FAPA; FAPalpha; SIMP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004460 edited
  GAATTCGGCACGAGGCCTTTTCAACGGTTTTCACAGATCCAGTGACCCACGCTCTGAAGA
  CAGAATTAGCTAACTTTCAAAAACATCTGGAAAAATGAAGACTTGGGTAAAAATCGTATT
  TGGAGTTGCCACCTCTGCTGTGCTTGCCTTATTGGTGATGTGCATTGTCTTACGCCCTTC
  AAGAGTTCATAACTCTGAAGAAAATACAATGAGAGCACTCACACTGAAGGATATTTTAAA
  TGGAACATTTTCTTATAAAACATTTTTTCCAAACTGGATTTCAGGACAAGAATATCTTCA
  TCAATCTGCAGATAACAATATAGTACTTTATAATATTGAAACAGGACAATCATATACCAT
  TTTGAGTAATAGAACCATGAAAAGTGTGAATGCTTCAAATTACGGCTTATCACCTGATCG
  GCAATTTGTATATCTAGAAAGTGATTATTCAAAGCTTTGGAGATACTCTTACACAGCAAC
  ATATTACATCTATGACCTTAGCAATGGAGAATTTGTAAGAGGAAATGAGCTTCCTCGTCC
  AATTCAGTATTTATGCTGGTCGCCTGTTGGGAGTAAATTAGCATATGTCTATCAAAACAA
  TATCTATTTGAAACAAAGACCAGGAGATCCACCTTTTCAAATAACATTTAATGGAAGAGA
  AAATAAAATATTTAATGGAATCCCAGACTGGGTTTATGAAGAGGAAATGCTTGCTACAAA
  ATATGCTCTCTGGTGGTCTCCTAATGGAAAATTTTTGGCATATGCGGAATTTAATGATAC
  GGATATACCAGTTATTGCCTATTCCTATTATGGCGATGAACAATATCCTAGAACAATAAA
  TATTCCATACCCAAAGGCTGGAGCTAAGAATCCCGTTGTTCGGATATTTATTATCGATAC
  CACTTACCCTGCGTATGTAGGTCCCCAGGAAGTGCCTGTTCCAGCAATGATAGCCTCAAG
  TGATTATTATTTCAGTTGGCTCACGTGGGTTACTGATGAACGAGTATGTTTGCAGTGGCT
  AAAAAGAGTCCAGAATGTTTCGGTCCTGTCTATATGTGACTTCAGGGAAGACTGGCAGAC
  ATGGGATTGTCCAAAGACCCAGGAGCATATAGAAGAAAGCAGAACTGGATGGGCTGGTGG
  ATTCTTTGTTTCAACACCAGTTTTCAGCTATGATGCCATTTCGTACTACAAAATATTTAG
  TGACAAGGATGGCTACAAACATATTCACTATATCAAAGACACTGTGGAAAATGCTATTCA
  AATTACAAGTGGCAAGTGGGAGGCCATAAATATATTCAGAGTAACACAGGATTCACTGTT
  TTATTCTAGCAATGAATTTGAAGAATACCCTGGAAGAAGAAACATCTACAGAATTAGCAT
  TGGAAGCTATCCTCCAAGCAAGAAGTGTGTTACTTGCCATCTAAGGAAAGAAAGGTGCCA
  ATATTACACAGCAAGTTTCAGCGACTACGCCAAGTACTATGCACTTGTCTGCTACGGCCC
  AGGCATCCCCATTTCCACCCTTCATGATGGACGCACTGATCAAGAAATTAAAATCCTGGA
  AGAAAACAAGGAATTGGAAAATGCTTTGAAAAATATCCAGCTGCCTAAAGAGGAAATTAA
  GAAACTTGAAGTAGATGAAATTACTTTATGGTACAAGATGATTCTTCCTCCTCAATTTGA
  CAGATCAAAGAAGTATCCCTTGCTAATTCAAGTGTATGGTGGTCCCTGCAGTCAGAGTGT
  AAGGTCTGTATTTGCTGTTAATTGGATATCTTATCTTGCAAGTAAGGAAGGGATGGTCAT
  TGCCTTGGTGGATGGTCGAGGAACAGCTTTCCAAGGTGACAAACTCCTCTATGCAGTGTA
  TCGAAAGCTGGGTGTTTATGAAGTTGAAGACCAGATTACAGCTGTCAGAAAATTCATAGA
  AATGGGTTTCATTGATGAAAAAAGAATAGCCATATGGGGCTGGTCCTATGGAGGATACGT
  TTCATCACTGGCCCTTGCATCTGGAACTGGTCTTTTCAAATGTGGTATAGCAGTGGCTCC
  AGTCTCCAGCTGGGAATATTACGCGTCTGTCTACACAGAGAGATTCATGGGTCTCCCAAC
  AAAGGATGATAATCTTGAGCACTATAAGAATTCAACTGTGATGGCAAGAGCAGAATATTT
  CAGAAATGTAGACTATCTTCTCATCCACGGAACAGCAGATGATAATGTGCACTTTCAAAA
  CTCAGCACAGATTGCTAAAGCTCTGGTTAATGCACAAGTGGATTTCCAGGCAATGTGGTA
  CTCTGACCAGAACCACGGCTTATCCGGCCTGTCCACGAACCACTTATACACCCACATGAC
  CCACTTCCTAAAGCAGTGTTTCTCTTTGTCAGACTAAAAACGATGCAGATGCAAGCCTGT
  ATCAGAATCTGAAAACCTTATATAAACCCCTCAGACAGTTTGCTTATTTTATTTTTTATG
  TTGTAAAATGCTAGTATAAACAAACAAATTAATGTTGTTCTAAAGGCTGTTAAAAAAAAG
  ATGAGGACTCAGAAGTTCAAGCTAAATATTGTTTACATTTTCTGGTACTCTGTGAAAGAA
  GAGAAAAGGGAGTCATGCATTTTGCTTTGGACACAGTGTTTTATCACCTGTTCATTTGAA
  GAAAAATAATAAAGTCAGAAGTTCAAGTGCTAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004460 unedited
  GGGTTTGAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGCCTTTTCA
  ACGGTTTTCACANATCCAGTGACCCACGCTCTGAAGACAGAATTAGCTAACTTTCAAAAN
  ACATCTGGAAAAATGAAGACTTGGGTAAAAATCGTATTTGGAGTTGCCACCTCTGCTGTG
  CTTGCCTTATTGGTGATGTGCATTGTCTTACGCCCTTCAAGAGTTCATAACTCTGAAGAA
  AATACAATGAGAGCACTCACACTGAAGGATATTTTAAATGGAACATTTTCTTATAAAACA
  TTTTTTCCAAACTGGATTTCAGGACAAGAATATCTTCATCAATCTGCAGATAACAATATA
  GTACTTTATAATATTGAAACAGGACAATCATATACCATTTTGAGTAATAGAACCATGAAA
  AGTGTGAATGCTTCAAATTACGGCTTATCACCTGATCGGCAATTTGTATATCTAGAAAGT
  GATTATTCAAAGCTTTGGAGATACTCCTTACACAGCAACATATTACATCTATGACCTTAG
  CAATGGAGAATTTGTAAGAGGAAATGAGCTTCCTCGTCCAATTCAGTATTTATGCTGCTC
  GCCTGTTGGGAGTAAATTAGCATAATGTCTATCAAAACCATATCTATTTGAAACAAAGAC
  CAGAAGATCCACCTTTTNAAATAACATTTTATGGAAGAGAAAATAAAATATTTTATGGAA
  TCCCAGACTGGGTTTATGAAGAGGAAATGCCTGCTACAAAAATGCTCTTTGGAGCGCTCC
  TACAGGAAATTTTTGGCACATGCCGCACTTAACGACACCGAATACCCGTTTTTGCCTATT
  CCTATTTAGGCGATGAACCATTTCCTAAAACATAAACCTCCCATCACCCAGGGCTCGCAC
  CTAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004460 unedited
  CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTAGCACTTGAACTTCTGACTT
  TATTATTTTTCTTCAAATGAACAGGTGATAAAACACTGTGTCCAAAGCAAAATGCATGAC
  TCCCTTTTCTCTTCTTTCACAGAGTACCAGAAAATGTAAACAATATTTAGCTTGAACTTC
  TGAGTCCTCATCTTTTTTTTAACAGCCTTTAGAACAACATTAATTTGTTTGTTTATACTA
  GCATTTTACAACATAAAAAATAAAATAAGCAAACTGTCTGAGGGGTTTATATAAGGTTTT
  CAGATTCTGATACAGGCTTGCATCTGCATCGTTTTTAGTCTGACAAAGAGAAACACTGCT
  TTAGGAAGTGGGTCATGTGGGTGTATAAGTGGTTCGTGGACAGGCCGGATAAGCCGTGGT
  TCTGGTCAGAGTACCACATTGCCTGGAAATCCACTTGTGCATTAACCAGAGCTTTAGCAA
  TCTGTGCTGAGTTTTGAAAGTGCACATTATCATCTGCTGTTCCGTGGATGAGAAGATAGT
  CTACATTTCTGAAATATTCTGCTCTTGCCATCACAGTTGAATTCTTATAGTGCTCAAGAT
  TATCATCCTTTGTTGGGAGACCCATGAATCTCTCTGTGTAGACAGACGCGTAATATTCCC
  AGCTGGAGACTGGAGCCACTGCTATACCACATTTGAAAAGACCAGTTCCAGATGCAAAGG
  CCAGTGATGAAACGTATCCTCCATAGGACCAGCCCCTATGGCTATCCTTTTTTATAATGA
  AACCCATTTCTATGAATTTTCTGACAGCGGAAATCTGCCTTCAAATTCTTAAACACCCAG
  CTTTGAATCCCTGCATTGAGGAGTTGGCACCTTGGAAACCTGTCCTTCACCATCACCAGG
  CAATGACCTCCCTTCCCTACTGCAGAAAAAATTCCATTTCCACCAACCCGCCTTCCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004460
• Insert Size: 2620 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004460.2, NP_004451.2
• RefSeq Size: 2788 bp
• RefSeq ORF: 2283 bp
• Locus ID: 2191
• Cytogenetics: 2q24.2
• Domains: Peptidase_S9, DPPIV_N_term
• Protein Families: Druggable Genome, Protease, Transmembrane
• Gene Summary: 'The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).