Fibroblast activation protein, alpha (FAP) (NM_004460) Human Untagged Clone

CAT#: SC117372

FAP (untagged)-Human fibroblast activation protein, alpha (FAP)


  "NM_004460" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol FAP
Synonyms DPPIV; FAPA; FAPalpha; SIMP
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_004460 edited
GAATTCGGCACGAGGCCTTTTCAACGGTTTTCACAGATCCAGTGACCCACGCTCTGAAGA
CAGAATTAGCTAACTTTCAAAAACATCTGGAAAAATGAAGACTTGGGTAAAAATCGTATT
TGGAGTTGCCACCTCTGCTGTGCTTGCCTTATTGGTGATGTGCATTGTCTTACGCCCTTC
AAGAGTTCATAACTCTGAAGAAAATACAATGAGAGCACTCACACTGAAGGATATTTTAAA
TGGAACATTTTCTTATAAAACATTTTTTCCAAACTGGATTTCAGGACAAGAATATCTTCA
TCAATCTGCAGATAACAATATAGTACTTTATAATATTGAAACAGGACAATCATATACCAT
TTTGAGTAATAGAACCATGAAAAGTGTGAATGCTTCAAATTACGGCTTATCACCTGATCG
GCAATTTGTATATCTAGAAAGTGATTATTCAAAGCTTTGGAGATACTCTTACACAGCAAC
ATATTACATCTATGACCTTAGCAATGGAGAATTTGTAAGAGGAAATGAGCTTCCTCGTCC
AATTCAGTATTTATGCTGGTCGCCTGTTGGGAGTAAATTAGCATATGTCTATCAAAACAA
TATCTATTTGAAACAAAGACCAGGAGATCCACCTTTTCAAATAACATTTAATGGAAGAGA
AAATAAAATATTTAATGGAATCCCAGACTGGGTTTATGAAGAGGAAATGCTTGCTACAAA
ATATGCTCTCTGGTGGTCTCCTAATGGAAAATTTTTGGCATATGCGGAATTTAATGATAC
GGATATACCAGTTATTGCCTATTCCTATTATGGCGATGAACAATATCCTAGAACAATAAA
TATTCCATACCCAAAGGCTGGAGCTAAGAATCCCGTTGTTCGGATATTTATTATCGATAC
CACTTACCCTGCGTATGTAGGTCCCCAGGAAGTGCCTGTTCCAGCAATGATAGCCTCAAG
TGATTATTATTTCAGTTGGCTCACGTGGGTTACTGATGAACGAGTATGTTTGCAGTGGCT
AAAAAGAGTCCAGAATGTTTCGGTCCTGTCTATATGTGACTTCAGGGAAGACTGGCAGAC
ATGGGATTGTCCAAAGACCCAGGAGCATATAGAAGAAAGCAGAACTGGATGGGCTGGTGG
ATTCTTTGTTTCAACACCAGTTTTCAGCTATGATGCCATTTCGTACTACAAAATATTTAG
TGACAAGGATGGCTACAAACATATTCACTATATCAAAGACACTGTGGAAAATGCTATTCA
AATTACAAGTGGCAAGTGGGAGGCCATAAATATATTCAGAGTAACACAGGATTCACTGTT
TTATTCTAGCAATGAATTTGAAGAATACCCTGGAAGAAGAAACATCTACAGAATTAGCAT
TGGAAGCTATCCTCCAAGCAAGAAGTGTGTTACTTGCCATCTAAGGAAAGAAAGGTGCCA
ATATTACACAGCAAGTTTCAGCGACTACGCCAAGTACTATGCACTTGTCTGCTACGGCCC
AGGCATCCCCATTTCCACCCTTCATGATGGACGCACTGATCAAGAAATTAAAATCCTGGA
AGAAAACAAGGAATTGGAAAATGCTTTGAAAAATATCCAGCTGCCTAAAGAGGAAATTAA
GAAACTTGAAGTAGATGAAATTACTTTATGGTACAAGATGATTCTTCCTCCTCAATTTGA
CAGATCAAAGAAGTATCCCTTGCTAATTCAAGTGTATGGTGGTCCCTGCAGTCAGAGTGT
AAGGTCTGTATTTGCTGTTAATTGGATATCTTATCTTGCAAGTAAGGAAGGGATGGTCAT
TGCCTTGGTGGATGGTCGAGGAACAGCTTTCCAAGGTGACAAACTCCTCTATGCAGTGTA
TCGAAAGCTGGGTGTTTATGAAGTTGAAGACCAGATTACAGCTGTCAGAAAATTCATAGA
AATGGGTTTCATTGATGAAAAAAGAATAGCCATATGGGGCTGGTCCTATGGAGGATACGT
TTCATCACTGGCCCTTGCATCTGGAACTGGTCTTTTCAAATGTGGTATAGCAGTGGCTCC
AGTCTCCAGCTGGGAATATTACGCGTCTGTCTACACAGAGAGATTCATGGGTCTCCCAAC
AAAGGATGATAATCTTGAGCACTATAAGAATTCAACTGTGATGGCAAGAGCAGAATATTT
CAGAAATGTAGACTATCTTCTCATCCACGGAACAGCAGATGATAATGTGCACTTTCAAAA
CTCAGCACAGATTGCTAAAGCTCTGGTTAATGCACAAGTGGATTTCCAGGCAATGTGGTA
CTCTGACCAGAACCACGGCTTATCCGGCCTGTCCACGAACCACTTATACACCCACATGAC
CCACTTCCTAAAGCAGTGTTTCTCTTTGTCAGACTAAAAACGATGCAGATGCAAGCCTGT
ATCAGAATCTGAAAACCTTATATAAACCCCTCAGACAGTTTGCTTATTTTATTTTTTATG
TTGTAAAATGCTAGTATAAACAAACAAATTAATGTTGTTCTAAAGGCTGTTAAAAAAAAG
ATGAGGACTCAGAAGTTCAAGCTAAATATTGTTTACATTTTCTGGTACTCTGTGAAAGAA
GAGAAAAGGGAGTCATGCATTTTGCTTTGGACACAGTGTTTTATCACCTGTTCATTTGAA
GAAAAATAATAAAGTCAGAAGTTCAAGTGCTAAAAAAAAAAAAAAAAACTCGAC
>OriGene 5' read for NM_004460 unedited
GGGTTTGAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGCCTTTTCA
ACGGTTTTCACANATCCAGTGACCCACGCTCTGAAGACAGAATTAGCTAACTTTCAAAAN
ACATCTGGAAAAATGAAGACTTGGGTAAAAATCGTATTTGGAGTTGCCACCTCTGCTGTG
CTTGCCTTATTGGTGATGTGCATTGTCTTACGCCCTTCAAGAGTTCATAACTCTGAAGAA
AATACAATGAGAGCACTCACACTGAAGGATATTTTAAATGGAACATTTTCTTATAAAACA
TTTTTTCCAAACTGGATTTCAGGACAAGAATATCTTCATCAATCTGCAGATAACAATATA
GTACTTTATAATATTGAAACAGGACAATCATATACCATTTTGAGTAATAGAACCATGAAA
AGTGTGAATGCTTCAAATTACGGCTTATCACCTGATCGGCAATTTGTATATCTAGAAAGT
GATTATTCAAAGCTTTGGAGATACTCCTTACACAGCAACATATTACATCTATGACCTTAG
CAATGGAGAATTTGTAAGAGGAAATGAGCTTCCTCGTCCAATTCAGTATTTATGCTGCTC
GCCTGTTGGGAGTAAATTAGCATAATGTCTATCAAAACCATATCTATTTGAAACAAAGAC
CAGAAGATCCACCTTTTNAAATAACATTTTATGGAAGAGAAAATAAAATATTTTATGGAA
TCCCAGACTGGGTTTATGAAGAGGAAATGCCTGCTACAAAAATGCTCTTTGGAGCGCTCC
TACAGGAAATTTTTGGCACATGCCGCACTTAACGACACCGAATACCCGTTTTTGCCTATT
CCTATTTAGGCGATGAACCATTTCCTAAAACATAAACCTCCCATCACCCAGGGCTCGCAC
CTAG
>OriGene 3' read for NM_004460 unedited
CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTAGCACTTGAACTTCTGACTT
TATTATTTTTCTTCAAATGAACAGGTGATAAAACACTGTGTCCAAAGCAAAATGCATGAC
TCCCTTTTCTCTTCTTTCACAGAGTACCAGAAAATGTAAACAATATTTAGCTTGAACTTC
TGAGTCCTCATCTTTTTTTTAACAGCCTTTAGAACAACATTAATTTGTTTGTTTATACTA
GCATTTTACAACATAAAAAATAAAATAAGCAAACTGTCTGAGGGGTTTATATAAGGTTTT
CAGATTCTGATACAGGCTTGCATCTGCATCGTTTTTAGTCTGACAAAGAGAAACACTGCT
TTAGGAAGTGGGTCATGTGGGTGTATAAGTGGTTCGTGGACAGGCCGGATAAGCCGTGGT
TCTGGTCAGAGTACCACATTGCCTGGAAATCCACTTGTGCATTAACCAGAGCTTTAGCAA
TCTGTGCTGAGTTTTGAAAGTGCACATTATCATCTGCTGTTCCGTGGATGAGAAGATAGT
CTACATTTCTGAAATATTCTGCTCTTGCCATCACAGTTGAATTCTTATAGTGCTCAAGAT
TATCATCCTTTGTTGGGAGACCCATGAATCTCTCTGTGTAGACAGACGCGTAATATTCCC
AGCTGGAGACTGGAGCCACTGCTATACCACATTTGAAAAGACCAGTTCCAGATGCAAAGG
CCAGTGATGAAACGTATCCTCCATAGGACCAGCCCCTATGGCTATCCTTTTTTATAATGA
AACCCATTTCTATGAATTTTCTGACAGCGGAAATCTGCCTTCAAATTCTTAAACACCCAG
CTTTGAATCCCTGCATTGAGGAGTTGGCACCTTGGAAACCTGTCCTTCACCATCACCAGG
CAATGACCTCCCTTCCCTACTGCAGAAAAAATTCCATTTCCACCAACCCGCCTTCCCA
Restriction Sites NotI-NotI     
ACCN NM_004460
Insert Size 2620 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004460.2, NP_004451.2
RefSeq Size 2788 bp
RefSeq ORF 2283 bp
Locus ID 2191
Cytogenetics 2q24.2
Domains Peptidase_S9, DPPIV_N_term
Protein Families Druggable Genome, Protease, Transmembrane
Gene Summary 'The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]'
Transcript Variant: This variant (1) encodes the longer isoform (1).

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