GCSH (NM_004483) Human Untagged Clone

CAT#: SC117387

GCSH (untagged)-Human glycine cleavage system protein H (aminomethyl carrier) (GCSH), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GCSH
• Synonyms: GCE; NKH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117387 sequence for NM_004483 edited (data generated by NextGen Sequencing)
  ATGGCGCTGCGAGTGGTGCGGAGCGTGCGGGCCCTGCTCTGCACCCTGCGCGCGGTCCCG
  TTACCCGCCGCGCCCTGCCCGCCGAGGCCCTGGCAGCTGGGGGTGGGCGCCGTCCGTACG
  CTGCGCACTGGACCCGCTCTGCTCTCGGTGCGTAAATTCACAGAGAAACACGAATGGGTA
  ACAACAGAAAATGGCATTGGAACAGTGGGAATCAGCAATTTTGCACAGGAAGCGTTGGGA
  GATGTTGTTTATTGTAGTCTCCCTGAAGTTGGGACAAAATTGAACAAACAAGATGAGTTT
  GGTGCTTTGGAAAGTGTGAAAGCTGCTAGTGAACTCTATTCTCCTTTATCAGGAGAAGTA
  ACTGAAATTAATGAAGCTCTTGCAGAAAATCCAGGACTTGTAAACAAATCTTGTTATGAA
  GATGGTTGGCTGATCAAGATGACACTGAGTAACCCTTCAGAACTAGATGAACTTATGAGT
  GAAGAAGCATATGAGAAATACATAAAATCTATTGAGGAGTGA
  
  Clone variation with respect to NM_004483.4
  62 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004483 unedited
  TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCAAGAGGCCGCACCCCTGCGAA
  CATGGCGCTGCGAGTGGTGCGGAGCGTGCGGGCCCTGCTCTGCACCCTGCGCGCGGTCCC
  GTTACCCGCCGCGCCCTGCCCGCCGAGGCCCTGGCAGCTGGGGGTGGGCGCCGTCCGTAC
  GCTGCGCACTGGACCCGCTCTGCTCTCGGTGCGTAAATTCACAGAGAAACACGAATGGGT
  AACAACAGAAAATGGCATTGGAACAGTGGGAATCAGCAATTTTGCACAGGAAGCGTTGGG
  AGATGTTGTTTATTGTAGTCTCCCTGAAGTTGGGACAAAATTGAACAAACAAGATGAGTT
  TGGTGCTTTGGAAAGTGTGAAAGCTGCTAGTGAACTCTATTCTCCTTTATCAGGAGAAGT
  AACTGAAATTAATGAAGCTCTTGCAGAAAATCCAGGACTTGTAAACAAATCTTGTTATGA
  AGATGGTTGGCTGATCAAGATGACACTGAGTAACCCTTCAGAACTAGATGAACTTATGAG
  TGAAGAAGCATATGAGAAATACATAAAATCTATTGAGGAGTGAAAATGGAACTCCTAAAT
  AAACTAGTATGAAATAACGCAAGCCAGCAGAGTTGTCTTAAATTAGTGGTGGATAGAAGA
  CTTAGAATAGAACTTTTAGTATTACCGATGGGGAAAAAAAAACTACTGTTAACACTGCTA
  ATGAAAGAAAATGCCCTTTAACTNTCTAATGATTATAGATAAATATAANTATGCGTCTTT
  NTCACAATATCCTATGATTNNTAGACTANGCTNCTAGTGGTCAGAAATCATNNGAATATN
  CATGGNTANAACTANGTATAAAAATTACATAATTCAAGATNACATTGNTATNCNTAAGCC
  TATNNTATATNGNCACTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004483 unedited
  CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTAACGAAGAAGTATTT
  TATTATTTTGCTGCAAAGCTGTTGCTTCACTGTATAAAAATAGCACCAGCAAATGCAGTG
  TATCGCAAAATTAAGATAGTGGTGTTCCTCATCTGACACTGTACAAGCAACAAAACCTCT
  TCACTTCCAGTTATTTCCAATGGAAAGATCATTAAGTATTTCATCCCAAATCCAGGTATG
  GATACATGCAAGTTACAATATTATATAAGGCTTAAGAATAACAATGTTATCTTTGAATTA
  TGTAATTTTTATAACTAGTTTTTACCATGGATAATTTCATGAATTCTGAACACTAGAGCC
  TAGTCTAAAAATCATAGGATATTGTGAAAAAGACGCATATTATATTTATCTATAATCATT
  AGAAAGTTAAAGGGCATTTTCTTTCATTAGCAGTGTTAACAGTAGTTTTTTTTTCCCCAT
  CGGTAATACTAAAAGTTTCTATTCTAAGTCTTCTATCCACCACTAATTTAAGACAACTCT
  GCTGGCTTGCGTTATTTCATACTAGTTTATTTAGGAGTTCCATTTTCACTCCTCAATAGA
  TTTATGATTTCTCAATGCCTTTCACTCATAGTTCATCTAGTTCTGAAGGGTTACTCAGTG
  GCATTTTTGATCAGCCAACCATCTTCATACAAAATTTGTTTACAAGCCCTGGATTTTTTG
  CAGACTTCATAATTTCCGTACTTCCCCTGATAAAGAGAATAGAGTTACTTNCAGTTTTAC
  ACTTTCCAAAGCACCAAATATTTTGGTTGGTCAATTTGGCCCAACTTAGGGAAATACATA
  AAAACATTTCCAAGCGTTCTGGGAAAATGGGGATCCACTGTTCAAGCCATTTTGTTGGCC
  CCTCCCGTTTTCTTGAAATACCCCCAAAACAACGGTCCAGGCAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004483
• Insert Size: 1150 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004483.3, NP_004474.2
• RefSeq Size: 1161 bp
• RefSeq ORF: 522 bp
• Locus ID: 2653
• Cytogenetics: 16q23.2
• Domains: GCV_H
• Gene Summary: 'Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the H protein, which transfers the methylamine group of glycine from the P protein to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH). Two transcript variants, one protein-coding and the other probably not protein-coding,have been found for this gene. Also, several transcribed and non-transcribed pseudogenes of this gene exist throughout the genome.[provided by RefSeq, Jan 2010]'
  Transcript Variant: This variant (1) represents the longer transcript and is the protein-coding variant. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.