SREBP1 (SREBF1) (NM_004176) Human Untagged Clone

CAT#: SC117502

SREBF1 (untagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SREBF1
• Synonyms: bHLHd1; SREBP1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004176 edited
  GGCGGCGGCGACTGAGAGCCGGGGCCGCGGCGGCGCTCCCTAGGAAGGGCCGTACGAGGC
  GGCGGGCCCGGCGGGCCTCCCGGAGGAGGCGGCTGCGCCATGGACGAGCCACCCTTCAGC
  GAGGCGGCTTTGGAGCAGGCGCTGGGCGAGCCGTGCGATCTGGACGCGGCGCTGCTGACC
  GACATCGAAGACATGCTTCAGCTTATCAACAACCAAGATAGTGACTTCCCTGGCCTATTT
  GACCCACCCTATGCTGGGAGTGGGGCAGGGGGCACAGACCCTGCCAGCCCCGATACCAGC
  TCCCCAGGCAGCTTGTCTCCACCTCCTGCCACATTGAGCTCCTCTCTTGAAGCCTTCCTG
  AGCGGGCCGCAGGCAGCGCCCTCACCCCTGTCCCCTCCCCAGCCTGCACCCACTCCATTG
  AAGATGTACCCGTCCATGCCCGCTTTCTCCCCTGGGCCTGGTATCAAGGAAGAGTCAGTG
  CCACTGAGCATCCTGCAGACCCCCACCCCACAGCCCCTGCCAGGGGCCCTCCTGCCACAG
  AGCTTCCCAGCCCCAGCCCCACCGCAGTTCAGCTCCACCCCTGTGTTAGGCTACCCCAGC
  CCTCCGGGAGGCTTCTCTACAGGAAGCCCTCCCGGGAACACCCAGCAGCCGCTGCCTGGC
  CTGCCACTGGCTTCCCCGCCAGGGGTCCCGCCCGTCTCCTTGCACACCCAGGTCCAGAGT
  GTGGTCCCCCAGCAGCTACTGACAGTCACAGCTGCCCCCACGGCAGCCCCTGTAACGACC
  ACTGTGACCTCGCAGATCCAGCAGGTCCCGGTCCTGCTGCAGCCCCACTTCATCAAGGCA
  GACTCGCTGCTTCTGACAGCCATGAAGACAGATGGAGCCACTGTGAAGGCGGCAGGTCTC
  AGTCCCCTGGTCTCTGGCACCACTGTGCAGACAGGGCCTTTGCCGACCCTGGTGAGTGGC
  GGAACCATCTTGGCAACAGTCCCACTGGTCGTAGATGCGGAGAAGCTGCCTATCAACCGG
  CTCGCAGCTGGCAGCAAGGCCCCGGCCTCTGCCCAGAGCCGTGGAGAGAAGCGCACAGCC
  CACAACGCCATTGAGAAGCGCTACCGCTCCTCCATCAATGACAAAATCATTGAGCTCAAG
  GATCTGGTGGTGGGCACTGAGGCAAAGCTGAATAAATCTGCTGTCTTGCGCAAGGCCATC
  GACTACATTCGCTTTCTGCAACACAGCAACCAGAAACTCAAGCAGGAGAACCTAAGTCTG
  CGCACTGCTGTCCACAAAAGCAAATCTCTGAAGGATCTGGTGTCGGCCTGTGGCAGTGGA
  GGGAACACAGACGTGCTCATGGAGGGCGTGAAGACTGAGGTGGAGGACACACTGACCCCA
  CCCCCCTCGGATGCTGGCTCACCTTTCCAGAGCAGCCCCTTGTCCCTTGGCAGCAGGGGC
  AGTGGCAGCGGTGGCAGTGGCAGTGACTCGGAGCCTGACAGCCCAGTCTTTGAGGACAGC
  AAGGCAAAGCCAGAGCAGCGGCCGTCTCTGCACAGCCGGGGCATGCTGGACCGCTCCCGC
  CTGGCCCTGTGCACGCTCGTCTTCCTCTGCCTGTCCTGCAACCCCTTGGCCTCCTTGCTG
  GGGGCCCGGGGGCTTCCCAGCCCCTCAGATACCACCAGCGTCTACCATAGCCCTGGGCGC
  AACGTGCTGGGCACCGAGAGCAGAGATGGCCCTGGCTGGGCCCAGTGGCTGCTGCCCCCA
  GTGGTCTGGCTGCTCAATGGGCTGTTGGTGCTCGTCTCCTTGGTGCTTCTCTTTGTCTAC
  GGTGAGCCAGTCACACGGCCCCACTCAGGCCCCGCCGTGTACTTCTGGAGGCATCGCAAG
  CAGGCTGACCTGGACCTGGCCCGGGGAGACTTTGCCCAGGCTGCCCAGCAGCTGTGGCTG
  GCCCTGCGGGCACTGGGCCGGCCCCTGCCCACCTCCCACCTGGACCTGGCTTGTAGCCTC
  CTCTGGAACCTCATCCGTCACCTGCTGCAGCGTCTCTGGGTGGGCCGCTGGCTGGCAGGC
  CGGGCAGGGGGCCTGCAGCAGGACTGTGCTCTGCGAGTGGATGCTAGCGCCAGCGCCCGA
  GACGCAGCCCTGGTCTACCATAAGCTGCACCAGCTGCACACCATGGGGAAGCACACAGGC
  GGGCACCTCACTGCCACCAACCTGGCGCTGAGTGCCCTGAACCTGGCAGAGTGTGCAGGG
  GATGCCGTGTCTGTGGCGACGCTGGCCGAGATCTATGTGGCGGCTGCATTGAGAGTGAAG
  ACCAGTCTCCCACGGGCCTTGCATTTTCTGACACGCTTCTTCCTGAGCAGTGCCCGCCAG
  GCCTGCCTGGCACAGAGTGGCTCAGTGCCTCCTGCCATGCAGTGGCTCTGCCACCCCGTG
  GGCCACCGTTTCTTCGTGGATGGGGACTGGTCCGTGCTCAGTACCCCATGGGAGAGCCTG
  TACAGCTTGGCCGGGAACCCAGTGGACCCCCTGGCCCAGGTGACTCAGCTATTCCGGGAA
  CATCTCTTAGAGCGAGCACTGAACTGTGTGACCCAGCCCAACCCCAGCCCTGGGTCAGCT
  GATGGGGACAAGGAATTCTCGGATGCCCTCGGGTACCTGCAGCTGCTGAACAGCTGTTCT
  GATGCTGCGGGGGCTCCTGCCTACAGCTTCTCCATCAGTTCCAGCATGGCCACCACCACC
  GGCGTAGACCCGGTGGCCAAGTGGTGGGCCTCTCTGACAGCTGTGGTGATCCACTGGCTG
  CGGCGGGATGAGGAGGCGGCTGAGCGGCTGTGCCCGCTGGTGGAGCACCTGCCCCGGGTG
  CTGCAGGAGTCTGAGAGACCCCTGCCCAGGGCAGCTCTGCACTCCTTCAAGGCTGCCCGG
  GCCCTGCTGGGCTGTGCCAAGGCAGAGTCTGGTCCAGCCAGCCTGACCATCTGTGAGAAG
  GCCAGTGGGTACCTGCAGGACAGCCTGGCTACCACACCAGCCAGCAGCTCCATTGACAAG
  GCCGTGCAGCTGTTCCTGTGTGACCTGCTTCTTGTGGTGCGCACCAGCCTGTGGCGGCAG
  CAGCAGCCCCCGGCCCCGGCCCCAGCAGCCCAGGGCACCAGCAGCAGGCCCCAGGCTTCC
  GCCCTTGAGCTGCGTGGCTTCCAACGGGACCTGAGCAGCCTGAGGCGGCTGGCACAGAGC
  TTCCGGCCCGCCATGCGGAGGGTGTTCCTACATGAGGCCACGGCCCGGCTGATGGCGGGG
  GCCAGCCCCACACGGACACACCAGCTCCTCGACCGCAGTCTGAGGCGGCGGGCAGGCCCC
  GGTGGCAAAGGAGGCGCGGTGGCGGAGCTGGAGCCGCGGCCCACGCGGCGGGAGCACGCG
  GAGGCCTTGCTGCTGGCCTCCTGCTACCTGCCCCCCGGCTTCCTGTCGGCGCCCGGGCAG
  CGCGTGGGCATGCTGGCTGAGGCGGCGCGCACACTCGAGAAGCTTGGCGATCGCCGGCTG
  CTGCACGACTGTCAGCAGATGCTCATGCGCCTGGGCGGTGGGACCACTGTCACTTCCAGC
  TAGACCCCGTGTCCCCGGCCTCAGCACCCCTGTCTCTAGCCACTTTGGTCCCGTGCAGCT
  TCTGTCCTGCGTCGAAGCTTTGAAGGCCGAAGGCAGTGCAAGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004176 unedited
  CGAATTCACCGCCCGTTGNCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA
  GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
  CGAATTCGGCACGAGGCCGCCTCTGAGGCCAGGGCAGGACACGAACGCGCGGAGCGGCGG
  CGGCGACTGAGAGCCGGGGCCGCGGCGGCGCTCCCTAGGAAGGGCCGTACGAGGCGGCGG
  GCCCGGCGGGCCTCCCGGAGGAGGCGGCTGCGCCATGGACGAGCCACCCTTCAGCGAGGC
  GGCTTTGGAGCAGGCGCTGGGCGAGCCGTGCGATCTGGACGCGGCGCTGCTGACCGACAT
  CGAAGACATGCTTCAGCTTATCAACAACCAAGATAGTGACTTCCCTGGCCTATTTGACCC
  ACCCTATGCTGGGAGTGGGGCAGGGGGCACAGACCCTGCCAGCCCCGATACCAGCTCCCC
  AGGCAGCTTGTCTCCACCTCCTGCCACATTGAGCTCCTCTCTTGAAGCCTTCCTGAGCGG
  GCCGCAGGCAGCGCCCTCACCCCTGTCCCCTCCCCAGCCTGCACCCACTCCATTGAAGAT
  GTACCCGTCCATGCCCGCTTTCTCCCCTGGGCCTGGTATCAAGGAAGAGTCAGTGCCACT
  GAGCATCCTGCAGACCCCCACCCCACAGCCCCTGCCAGGGGCCCTCCTGCCACAGAGCTT
  NCCAGCCCCAGCCCCACCGCAGTTCAGCTCCACCCCTGTGTTAGGCTACCCCAGCCCTCC
  GGGAGGCTTCTCTACAGGAAGCCCCTCCGGGAACACCCAGCAGCCGCTGCCTGGCCTGCC
  ACTGGCTTCCCGNNCCAGGGTCCCGCCCGTCTCCTTGCCACCCAGTCCANAGAAGGGTCC
  CCCAGCAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004176 unedited
  NCGACGGAAATACTATGTACCGCGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAATTTCATTTTTAAT
  TCTCTGGACAAAACTTCTCAATCTATGAAAATAAAGTTTGCAAAAGGCAAAGTACCCCAG
  GAGCCTCAGGTCAGGAGGCTAAACCCCCTGACCTACACTATGTACACGTTTCTCTCCCAC
  AAAGGAAAAAAAGGCCTCTGGGGCAAAACCCTGCTTGCAGTCCGGGAAAACCCAATTGGC
  TTCCGTCAGCCCAGGGAAATGTACCCCTCTTTTCCCTGGTCCCCGGGGGAAAAAAGCCCC
  TTAGGGGCCCGCAAAACAAGGAGGGGGGCCCCCCAAAATGGGTCGGCCCCTGGAGTGCCC
  CGATTGGCCCCCCAAGGTCAACCCCCTTTTGGCCCCCCGTCTTAAGGTCAAAAATGCGCC
  CCTTGGGCCCTCCACCGGGAAAGCCCCCAACAACCCCCGGTCCAACTGGGGGGGCCCAAA
  TTTTTTGGGCTGGCTTTGGCCTTTAAAGCTTTCACCCCAGACAAAAACCGCCCCGGACCC
  AAATGGCTTTAAACAGGGGTGCCTTAAGCCCGGGACCCCGGGTCCAACCGGGAAATGACA
  AAGGGGCCCCCCCCCCCAGCCCAATGAACATTTTGTTGAAAATTCTGGCAAAAACCCGGG
  ATTCCCCCAACTTTTTTGAGGTGGGCCCCCCCCCCCTTAACCAAATATCCCCACCCCCTT
  TCCCGGGCCCCCAAAAGAAAACCCGGGGGCCGGTTTCAAGGAGGGCCCACTACAAGGCCC
  CCCCGGGGCTCCCCCCCCGGGGGGCCGGGGTTTCAATTTTCCCACCCGCCCCCCTTTGTC
  C
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004176
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004176.3, NP_004167.3
• RefSeq Size: 4194 bp
• RefSeq ORF: 3444 bp
• Locus ID: 6720
• Cytogenetics: 17p11.2
• Domains: HLH
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Insulin signaling pathway
• Gene Summary: 'This gene encodes a basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a motif that is found in the promoter of the low density lipoprotein receptor gene and other genes involved in sterol biosynthesis. The encoded protein is synthesized as a precursor that is initially attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription. This cleaveage is inhibited by sterols. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative promoter usage and splicing result in multiple transcript variants, including SREBP-1a and SREBP-1c, which correspond to RefSeq transcript variants 2 and 3, respectively. [provided by RefSeq, Nov 2017]'
  Transcript Variant: This variant (2, also known as SREBP-1a) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b, or SREBP-1a) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.