Annexin VII (ANXA7) (NM_004034) Human Untagged Clone

CAT#: SC117619

ANXA7 (untagged)-Human annexin A7 (ANXA7), transcript variant 2


  "NM_004034" in other vectors (4)

Reconstitution Protocol

USD 820.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ANXA7
Synonyms ANX7; SNX; SYNEXIN
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC117619 sequence for NM_004034 edited (data generated by NextGen Sequencing)
ATGTCATACCCAGGCTATCCCCCAACAGGCTACCCACCTTTCCCTGGATATCCTCCTGCA
GGTCAGGAGTCATCTTTTCCCCCTTCTGGTCAGTATCCTTATCCTAGTGGCTTTCCTCCA
ATGGGAGGAGGTGCCTACCCACAAGTGCCAAGTAGTGGCTACCCAGGAGCTGGAGGCTAC
CCTGCGCCTGGAGGTTATCCAGCCCCTGGAGGCTATCCTGGTGCCCCACAGCCAGGGGGA
GCTCCATCCTATCCCGGAGTTCCTCCAGGCCAAGGATTTGGAGTCCCACCAGGTGGAGCA
GGCTTTTCTGGGTATCCACAGCCACCTTCACAGTCTTATGGAGGTGGTCCAGCACAGGTT
CCACTACCTGGTGGCTTTCCTGGAGGACAGATGCCTTCTCAGTATCCTGGAGGACAACCT
ACTTACCCTAGTCAGATCAATACAGATTCTTTTTCTTCCTATCCTGTTTTCTCTCCTGTT
TCTTTGGATTATAGCAGTGAACCTGCCACAGTGACTCAGGTCACTCAAGGAACTATCCGA
CCAGCTGCCAACTTCGATGCTATAAGAGATGCAGAAATTCTTCGTAAGGCAATGAAGGGT
TTTGGGACAGATGAGCAGGCAATTGTGGATGTGGTGGCCAACCGTTCCAATGATCAGAGG
CAAAAAATTAAAGCAGCATTTAAGACCTCCTATGGCAAGGATTTAATCAAAGATCTCAAA
TCAGAGTTAAGTGGAAATATGGAAGAACTGATCCTGGCCCTCTTCATGCCTCCTACGTAT
TACGATGCCTGGAGCTTACGGAAAGCAATGCAGGGAGCAGGAACTCAGGAACGTGTATTG
ATTGAGATTTTGTGCACAAGAACAAATCAGGAAATCCGAGAAATTGTCAGATGTTATCAG
TCAGAATTTGGACGAGACCTTGAAAAGGACATTAGGTCAGATACATCAGGACATTTTGAA
CGTTTACTTGTGTCCATGTGCCAGGGAAATCGTGATGAGAACCAGAGTATAAACCACCAA
ATGGCTCAGGAAGATGCTCAGCGTCTCTATCAAGCTGGTGAGGGGAGACTAGGGACCGAT
GAATCTTGCTTTAACATGATCCTTGCCACAAGAAGCTTTCCTCAGCTGAGAGCTACCATG
GAGGCTTATTCTAGGATGGCTAATCGAGACTTGTTAAGCAGTGTGAGCCGTGAGTTTTCC
GGATATGTAGAAAGTGGTTTGAAGACCATCTTGCAGTGTGCCCTGAACCGCCCTGCCTTC
TTTGCTGAGAGGCTCTACTATGCTATGAAAGGTGCTGGCACAGATGACTCCACCCTGGTC
CGGATTGTGGTCACTCGAAGTGAGATTGACCTTGTACAAATAAAACAGATGTTCGCTCAG
ATGTATCAGAAGACTCTGGGCACAATGATTGCAGGTGACACGAGTGGAGATTACCGAAGA
CTTCTTCTGGCTATTGTGGGCCAGTAG

Clone variation with respect to NM_004034.2
>OriGene 5' read for NM_004034 unedited
TTTGTAATACGACTCACTATAGGGCGGCCCGCGATTCGGCACGAGGTGTGACGCTGCTGC
TGGGGTCAGATTTTTTTTTCCTTTATTTTGACCCTGACCCTTTTGCCCTTGGTCTATCTA
CTTTTCTTTTTCTTTCTTTTTTTTTTTTTCTTTTTCCTTGTCTTGTCTTGTCTTTTCTTT
CAACTGGAGTGCAATGATGCAAACACGACTCACTGCCGCCTTGACCTCCTGGCCTCAAGC
AATTCTCCCGCCTCAGCTTCCTGAGTAGTTTGGACTACGGGTGGGCGCCGCTGCACTGGA
ATGTCATACCCAGGCTATCCCCCAACAGGCTACCCACCTTTCCCTGGATATCCTCCTGCA
GGTCAGGAGTCATCTTTTCCCCCTTCTGGTCAGTATCCTTATCCTAGTGGCTTTCCTCCA
ATGGGAGGAGGTGCCTACCCACAAGTGCCAAGTAGTGGCTACCCAGGAGCTGGAGGCTAC
CCTGCGCCTGGAGGTTATCCAGCCCCTGGAGGCTATCCTGGTGCCCCACAGCCAGGGGGA
GCTCCATCCTATCCCGGAGTTCCTCCAGGCCAAGGATTTGGAGTCCCACCAGGTGGAGCA
GGCTTTTCTGGGTATCCACAGCCACCTTCACAGTCTTATGNGAGGTGGTCCAGCACAGGT
TCCACTACCTNGTGGCTTTCCTGGAGGACAGATGCCTTCTCAGTATCCTGGAGGACAACC
TACTTACCCTAGTCAGATCAATACAGATNCTTTNTCTTCCTATCCTGTTTTCTCTCCTGG
TTCTTTGGATATAGCAGNGAACCTGCCACAGTGACTCANGTCACTCAAGGAACTATNCGA
CCAGCTGCCAACTTCGATGCTATAAGGATGCAGAATATCTTCTAAGCAATGAAGGNNTTT
NGGACAGAGAGCANGCATT
>OriGene 3' read for NM_004034 unedited
TGTCATAACTTAATTTAAAAAAGAAAACAACTGCGAAAAAAAAGTAGCTATGTATCTTAA
GCATATAAACATTTGACCCTTTCTTAAAAGGTGCCACAGGTAATACTAATCTATTTTCTA
TGTGGAAAGTGTTTATGAATACAGAACCAATTTAGGCAAAATGAGATGCAAATTATTCTA
GATGTAAAGGTTGAATTTTTTTTTAAAGTACACAGTTTTAACATACTATCCACTCTATCA
AGCAGACTTAGAGGGATTCCGGAAGGCAGAGCTTTCAGTAACCTTCTAAAAAATTATTCA
ATATTATTACACAGAATCAGAGATTGCTGTGATTTTTATTCAATTTGACATCTTGATTAG
AATGAAACTAGTAAGAATGAAGGTTTACAAACATTGCAATATTACTGTATCATTGTGATA
TGGCTTTACATTGATTGTATGTAGAGAACAAAATAAAATTAGAATTAAGGCAATAACAAC
ATGTGCAAACCAAGCACATTACCCTGATACGGTCCTTGACAGAAAGCTCTTTCGGTTAGC
TGATGTTTGATATTGCTGCATGCANGTCATTGCTCTGAAGGATAAGCTATGAATAGAAAA
TTTTTTTCATTAAAAAAAAAAAATCCCTCCTACTGGNCCCACATAGCCAGAAGAAGTCTT
TCGGTATCTNCACTCGTGTCACCTGCAATCATTGTGCCCAGAGTCTTCTGATACATCTGA
GCGAACATCTGTTNTATTTGTACAAGGGTCATCTCACTTCGAGTGACCACAATCCGGACA
NGGTGGAGTCATCTGTGCCAGCACCTTTCATAGCATAGTAAAGCCTCTCAGCAAAGAAGC
AGGGCGGTTCAGGCACACTGGCAGATGGTCTTCACCCCTTTTTACTATCCGGAACTAACG
TTACACTGTTTACAGTTTCGATAAGCATCCTAAAAAGCCTCCTGTACCTCTTACTGAGAA
GCTTCTGTGGGAAGATATGTTAAGCAAATTATTGGCCTATTCTCCTAACAACTGATTAGA
CCTGAGCCTTTCTGAGCATTGGGGGTTACCTTGGTTAAAACATTCCCGGAAGG
Restriction Sites NotI-NotI     
ACCN NM_004034
Insert Size 2640 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004034.1, NP_004025.1
RefSeq Size 2176 bp
RefSeq ORF 1467 bp
Locus ID 310
Cytogenetics 10q22.2
Domains annexin
Gene Summary 'Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3'-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.

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