Annexin VII (ANXA7) (NM_004034) Human Untagged Clone

CAT#: SC117619

ANXA7 (untagged)-Human annexin A7 (ANXA7), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ANXA7
• Synonyms: ANX7; SNX; SYNEXIN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117619 sequence for NM_004034 edited (data generated by NextGen Sequencing)
  ATGTCATACCCAGGCTATCCCCCAACAGGCTACCCACCTTTCCCTGGATATCCTCCTGCA
  GGTCAGGAGTCATCTTTTCCCCCTTCTGGTCAGTATCCTTATCCTAGTGGCTTTCCTCCA
  ATGGGAGGAGGTGCCTACCCACAAGTGCCAAGTAGTGGCTACCCAGGAGCTGGAGGCTAC
  CCTGCGCCTGGAGGTTATCCAGCCCCTGGAGGCTATCCTGGTGCCCCACAGCCAGGGGGA
  GCTCCATCCTATCCCGGAGTTCCTCCAGGCCAAGGATTTGGAGTCCCACCAGGTGGAGCA
  GGCTTTTCTGGGTATCCACAGCCACCTTCACAGTCTTATGGAGGTGGTCCAGCACAGGTT
  CCACTACCTGGTGGCTTTCCTGGAGGACAGATGCCTTCTCAGTATCCTGGAGGACAACCT
  ACTTACCCTAGTCAGATCAATACAGATTCTTTTTCTTCCTATCCTGTTTTCTCTCCTGTT
  TCTTTGGATTATAGCAGTGAACCTGCCACAGTGACTCAGGTCACTCAAGGAACTATCCGA
  CCAGCTGCCAACTTCGATGCTATAAGAGATGCAGAAATTCTTCGTAAGGCAATGAAGGGT
  TTTGGGACAGATGAGCAGGCAATTGTGGATGTGGTGGCCAACCGTTCCAATGATCAGAGG
  CAAAAAATTAAAGCAGCATTTAAGACCTCCTATGGCAAGGATTTAATCAAAGATCTCAAA
  TCAGAGTTAAGTGGAAATATGGAAGAACTGATCCTGGCCCTCTTCATGCCTCCTACGTAT
  TACGATGCCTGGAGCTTACGGAAAGCAATGCAGGGAGCAGGAACTCAGGAACGTGTATTG
  ATTGAGATTTTGTGCACAAGAACAAATCAGGAAATCCGAGAAATTGTCAGATGTTATCAG
  TCAGAATTTGGACGAGACCTTGAAAAGGACATTAGGTCAGATACATCAGGACATTTTGAA
  CGTTTACTTGTGTCCATGTGCCAGGGAAATCGTGATGAGAACCAGAGTATAAACCACCAA
  ATGGCTCAGGAAGATGCTCAGCGTCTCTATCAAGCTGGTGAGGGGAGACTAGGGACCGAT
  GAATCTTGCTTTAACATGATCCTTGCCACAAGAAGCTTTCCTCAGCTGAGAGCTACCATG
  GAGGCTTATTCTAGGATGGCTAATCGAGACTTGTTAAGCAGTGTGAGCCGTGAGTTTTCC
  GGATATGTAGAAAGTGGTTTGAAGACCATCTTGCAGTGTGCCCTGAACCGCCCTGCCTTC
  TTTGCTGAGAGGCTCTACTATGCTATGAAAGGTGCTGGCACAGATGACTCCACCCTGGTC
  CGGATTGTGGTCACTCGAAGTGAGATTGACCTTGTACAAATAAAACAGATGTTCGCTCAG
  ATGTATCAGAAGACTCTGGGCACAATGATTGCAGGTGACACGAGTGGAGATTACCGAAGA
  CTTCTTCTGGCTATTGTGGGCCAGTAG
  
  Clone variation with respect to NM_004034.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004034 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCCGCGATTCGGCACGAGGTGTGACGCTGCTGC
  TGGGGTCAGATTTTTTTTTCCTTTATTTTGACCCTGACCCTTTTGCCCTTGGTCTATCTA
  CTTTTCTTTTTCTTTCTTTTTTTTTTTTTCTTTTTCCTTGTCTTGTCTTGTCTTTTCTTT
  CAACTGGAGTGCAATGATGCAAACACGACTCACTGCCGCCTTGACCTCCTGGCCTCAAGC
  AATTCTCCCGCCTCAGCTTCCTGAGTAGTTTGGACTACGGGTGGGCGCCGCTGCACTGGA
  ATGTCATACCCAGGCTATCCCCCAACAGGCTACCCACCTTTCCCTGGATATCCTCCTGCA
  GGTCAGGAGTCATCTTTTCCCCCTTCTGGTCAGTATCCTTATCCTAGTGGCTTTCCTCCA
  ATGGGAGGAGGTGCCTACCCACAAGTGCCAAGTAGTGGCTACCCAGGAGCTGGAGGCTAC
  CCTGCGCCTGGAGGTTATCCAGCCCCTGGAGGCTATCCTGGTGCCCCACAGCCAGGGGGA
  GCTCCATCCTATCCCGGAGTTCCTCCAGGCCAAGGATTTGGAGTCCCACCAGGTGGAGCA
  GGCTTTTCTGGGTATCCACAGCCACCTTCACAGTCTTATGNGAGGTGGTCCAGCACAGGT
  TCCACTACCTNGTGGCTTTCCTGGAGGACAGATGCCTTCTCAGTATCCTGGAGGACAACC
  TACTTACCCTAGTCAGATCAATACAGATNCTTTNTCTTCCTATCCTGTTTTCTCTCCTGG
  TTCTTTGGATATAGCAGNGAACCTGCCACAGTGACTCANGTCACTCAAGGAACTATNCGA
  CCAGCTGCCAACTTCGATGCTATAAGGATGCAGAATATCTTCTAAGCAATGAAGGNNTTT
  NGGACAGAGAGCANGCATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004034 unedited
  TGTCATAACTTAATTTAAAAAAGAAAACAACTGCGAAAAAAAAGTAGCTATGTATCTTAA
  GCATATAAACATTTGACCCTTTCTTAAAAGGTGCCACAGGTAATACTAATCTATTTTCTA
  TGTGGAAAGTGTTTATGAATACAGAACCAATTTAGGCAAAATGAGATGCAAATTATTCTA
  GATGTAAAGGTTGAATTTTTTTTTAAAGTACACAGTTTTAACATACTATCCACTCTATCA
  AGCAGACTTAGAGGGATTCCGGAAGGCAGAGCTTTCAGTAACCTTCTAAAAAATTATTCA
  ATATTATTACACAGAATCAGAGATTGCTGTGATTTTTATTCAATTTGACATCTTGATTAG
  AATGAAACTAGTAAGAATGAAGGTTTACAAACATTGCAATATTACTGTATCATTGTGATA
  TGGCTTTACATTGATTGTATGTAGAGAACAAAATAAAATTAGAATTAAGGCAATAACAAC
  ATGTGCAAACCAAGCACATTACCCTGATACGGTCCTTGACAGAAAGCTCTTTCGGTTAGC
  TGATGTTTGATATTGCTGCATGCANGTCATTGCTCTGAAGGATAAGCTATGAATAGAAAA
  TTTTTTTCATTAAAAAAAAAAAATCCCTCCTACTGGNCCCACATAGCCAGAAGAAGTCTT
  TCGGTATCTNCACTCGTGTCACCTGCAATCATTGTGCCCAGAGTCTTCTGATACATCTGA
  GCGAACATCTGTTNTATTTGTACAAGGGTCATCTCACTTCGAGTGACCACAATCCGGACA
  NGGTGGAGTCATCTGTGCCAGCACCTTTCATAGCATAGTAAAGCCTCTCAGCAAAGAAGC
  AGGGCGGTTCAGGCACACTGGCAGATGGTCTTCACCCCTTTTTACTATCCGGAACTAACG
  TTACACTGTTTACAGTTTCGATAAGCATCCTAAAAAGCCTCCTGTACCTCTTACTGAGAA
  GCTTCTGTGGGAAGATATGTTAAGCAAATTATTGGCCTATTCTCCTAACAACTGATTAGA
  CCTGAGCCTTTCTGAGCATTGGGGGTTACCTTGGTTAAAACATTCCCGGAAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004034
• Insert Size: 2640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004034.1, NP_004025.1
• RefSeq Size: 2176 bp
• RefSeq ORF: 1467 bp
• Locus ID: 310
• Cytogenetics: 10q22.2
• Domains: annexin
• Gene Summary: 'Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3'-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.