Aurora A (AURKA) (NM_003600) Human Untagged Clone

CAT#: SC117877

AURKA (untagged)-Human aurora kinase A (AURKA), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AURKA
• Synonyms: AIK; ARK1; AURA; BTAK; PPP1R47; STK6; STK7; STK15
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003600 edited
  ATGGACCGATCTAAAGAAAACTGCATTTCAGGACCTGTTAAGGCTACAGCTCCAGTTGGA
  GGTCCAAAACGTGTTCTCGTGACTCAGCAATTTCCTTGTCAGAATCCATTACCTGTAAAT
  AGTGGCCAGGCTCAGCGGGTCTTGTGTCCTTCAAATTCTTCCCAGCGCGTTCCTTTGCAA
  GCACAAAAGCTTGTCTCCAGTCACAAGCCGGTTCAGAATCAGAAGCAGAAGCAATTGCAG
  GCAACCAGTGTACCTCATCCTGTCTCCAGGCCACTGAATAACACCCAAAAGAGCAAGCAG
  CCCCTGCCATCGGCACCTGAAAATAATCCTGAGGAGGAACTGGCATCAAAACAGAAAAAT
  GAAGAATCAAAAAAGAGGCAGTGGGCTTTGGAAGACTTTGAAATTGGTCGCCCTCTGGGT
  AAAGGAAAGTTTGGTAATGTTTATTTGGCAAGAGAAAAGCAAAGCAAGTTTATTCTGGCT
  CTTAAAGTGTTATTTAAAGCTCAGCTGGAGAAAGCCGGAGTGGAGCATCAGCTCAGAAGA
  GAAGTAGAAATACAGTCCCACCTTCGGCATCCTAATATTCTTAGACTGTATGGTTATTTC
  CATGATGCTACCAGAGTCTACCTAATTCTGGAATATGCACCACTTGGAACAGTTTATAGA
  GAACTTCAGAAACTTTCAAAGTTTGATGAGCAGAGAACTGCTACTTATATAACAGAATTG
  GCAAATGCCCTGTCTTACTGTCATTCGAAGAGAGTTATTCATAGAGACATTAAGCCAGAG
  AACTTACTTCTTGGATCAGCTGGAGAGCTTAAAATTGCAGATTTTGGGTGGTCAGTACAT
  GCTCCATCTTCCAGGAGGACCACTCTCTGTGGCACCCTGGACTACCTGCCCCCTGAAATG
  ATTGAAGGTCGGATGCATGATGAGAAGGTGGATCTCTGGAGCCTTGGAGTTCTTTGCTAT
  GAATTTTTAGTTGGGAAGCCTCCTTTTGAGGCAAACACATACCAAGAGACCTACAAAAGA
  ATATCACGGGTTGAATTCACATTCCCTGACTTTGTAACAGAGGGAGCCAGGGACCTCATT
  TCAAGACTGTTGAAGCATAATCCCAGCCAGAGGCCAATGCTCAGAGAAGTACTTGAACAC
  CCCTGGATCACAGCAAATTCATCAAAACCATCAAATTGCCAAAACAAAGAATCAGCTAGC
  AAACAGTCTTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003600 unedited
  AACTCGNGATTTGTAAAACGNACTTTCTATAGGCGGCAAAACCATTCGCACCAGGTCGCA
  GGTGGGAGCCGACGGGCATCATGGACCGATCTAAAGAAAACTGCATTTCAGGACCTGTTA
  AGGCTACAGCTCCAGTTGGAGGTCCAAAACGTGTTCTCGTGACTCAGCAATTTCCTTGTC
  AGAATCCATTACCTGTAAATAGTGGCCAGGCTCACTGTTTTTGTGTCCTTCAAATTCTTC
  CCAGCGCGTTCCTTTGCAAGCACAAAAGCTTGTCTCCAGTCACAAGCCGGTTCAGAATCA
  GAAGCAGAAGCAATTGCAGGCAACCAGTGTACCTCATCCTGTCTCCAGGCCACTGAATAA
  CACCCAAAAGAGCAAGCAGCCCCTGCCATCGGCACCTGAAAATAATCCTGAGGAGGAACT
  GGCATCAAAACAGAAAAATGAAGAATCAAAAAAGAGGCAGTGGGCTTTGGAAGACTTTGA
  AATTGGTCGCCCTCTGGGTAAAGGAAAGTTTGGTAATGTTTATTTGGCAAGAGAAAAGCA
  AAGCAAGTTTATTCTGGCTCTTAAAGTGTTATTTAAAGCTCAGCTGGAGAAAGCCGGAGT
  GGAGCATCAGCTCAGAAGAGAAGTAGAAATACAGTCCCACCTTCGGCATCCTAATATTCT
  TAGACTGTATGGTTATTTCCATGATGCTACCAGAGTCTACCTAATTCTGGAATATGCACC
  ACTTGGAACAGTTTATAGAGAACTTCAGAAACTTTTCAAGTTTGATGAGCAGAGAACTGC
  TACTTATATAACAAGATTGGGCAAATGCCTGTCTTACTGTCATTCGAAAAGAGTTATTCT
  TAGAGACATAAGCCAGGAGACTTACCTTCNGAATCAGCTGGAGAGCTTAAAATGCAGATT
  TTGGGGGGNCAGACCTGCTCCATCTTCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003600 unedited
  TTTTTTTTTTTTTTTTTNGACTAGNAACTTATTTATTTGCATATTTAAAGTTTACACACA
  TGCTATGACTCCAATGTTTTAAAAAAAAAGCCCTTAACAGCTCTGAGACACATGGCCTCT
  TCTGTATCCCAAGCAAATCCCTAAATGGAGGTAGAGCACGTGTTCCTATTTTTCACACTC
  TCATATGGGAGATAAGTGGTTAAGGAGGACTAGAAACCCAATCAGGCCTACCGGGGTGCC
  GGACAGACACACAGCATTCCTAAAGGAATGCCACCAGAGAAAAAATACAAGTCTGTACAT
  ATATCTTTATTTTCATACTTAAAAAGAATCACATACTCATTCCAACAGCTTTACCAGGCT
  TCGCCAACCCAATAAGTTACACACTCACTCAGGTACTAGGAAGGTTATTGCACAGCTCCT
  TAACTGATCGGGGTCAGGGCAGAGTGGTCACTTTCCCCACAGCCAGGCTCTGGATTTGCC
  TCCTGTGAAGACACCATGCCTAGCACAGGCTGACGGGGCGGCTGCAGTCGAACCTTGCCT
  CCAGATTATGAACCAGTATAAGTAGCACAATTCTCGTGGCTACTTTCACTTCAGAGTGTC
  ATGTTTATTGATGTGGAGCTTTCTGAATAGGGAGGTTAAGGCACACCTGCTGAGTAAAAC
  AATATTTCTTGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003600
• Insert Size: 1950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003600.2, NP_003591.2
• RefSeq Size: 2346 bp
• RefSeq ORF: 1212 bp
• Locus ID: 6790
• Cytogenetics: 20q13.2
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Oocyte meiosis
• Gene Summary: 'The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1-9 encode the same protein.