CRM1 (XPO1) (NM_003400) Human Untagged Clone

CAT#: SC118003

XPO1 (untagged)-Human exportin 1 (CRM1 homolog, yeast) (XPO1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: XPO1
• Synonyms: CRM-1; CRM1; emb; exp1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118003 sequence for NM_003400 edited (data generated by NextGen Sequencing)
  ATGCCAGCAATTATGACAATGTTAGCAGACCATGCAGCTCGTCAGCTGCTTGATTTCAGC
  CAAAAACTGGATATCAACTTATTAGATAATGTGGTGAATTGCTTATACCATGGAGAAGGA
  GCCCAGCAAAGAATGGCTCAAGAAGTACTGACACATTTAAAGGAGCATCCTGATGCTTGG
  ACAAGAGTCGACACAATTTTGGAATTTTCTCAGAATATGAATACGAAATACTATGGACTA
  CAAATTTTGGAAAATGTGATAAAAACAAGGTGGAAGATTCTTCCAAGGAACCAGTGCGAA
  GGAATAAAAAAATACGTTGTTGGCCTCATTATCAAGACGTCATCTGACCCAACTTGTGTA
  GAGAAAGAAAAGGTGTATATCGGAAAATTAAATATGATCCTTGTTCAGATACTGAAACAA
  GAATGGCCCAAACATTGGCCAACTTTTATCAGTGATATTGTTGGAGCAAGTAGGACCAGC
  GAAAGTCTCTGTCAAAATAATATGGTGATTCTTAAACTCTTGAGTGAAGAAGTATTTGAT
  TTCTCTAGTGGACAGATAACCCAAGTCAAATCTAAGCATTTAAAAGACAGCATGTGCAAT
  GAATTCTCACAGATATTTCAACTGTGTCAGTTTGTAATGGAAAATTCTCAAAATGCTCCA
  CTTGTACATGCAACCTTGGAAACATTGCTCAGATTTCTGAACTGGATTCCCCTGGGATAT
  ATTTTTGAGACCAAATTAATCAGCACATTGATTTATAAGTTCCTGAATGTTCCAATGTTT
  CGAAATGTCTCTCTGAAGTGCCTCACTGAGATTGCTGGTGTGAGTGTAAGCCAATATGAA
  GAACAATTTGTAACACTATTTACTCTGACAATGATGCAACTAAAGCAGATGCTTCCTTTA
  AATACCAATATTCGACTTGCGTACTCAAATGGAAAAGATGATGAACAGAACTTCATTCAA
  AATCTCAGTTTGTTTCTCTGCACCTTTCTTAAGGAACATGATCAACTTATAGAAAAAAGA
  TTAAATCTCAGGGAAACTCTTATGGAGGCCCTTCATTATATGTTGTTGGTATCTGAAGTA
  GAAGAAACTGAAATCTTTAAAATTTGTCTTGAATACTGGAATCATTTGGCTGCTGAACTC
  TATAGAGAGAGTCCATTCTCTACATCTGCCTCTCCGTTGCTTTCTGGAAGTCAACATTTT
  GATGTTCCTCCCAGGAGACAGCTATATTTGCCCATGTTATTCAAGGTCCGTTTATTAATG
  GTTAGTCGAATGGCTAAACCAGAGGAAGTATTGGTTGTAGAGAATGATCAAGGAGAAGTT
  GTGAGAGAATTCATGAAGGATACAGATTCCATAAATTTGTATAAGAATATGAGGGAAACA
  TTGGTTTATCTTACTCATCTGGATTATGTAGATACAGAAAGAATAATGACAGAGAAGCTT
  CACAATCAAGTGAATGGTACAGAGTGGTCATGGAAAAATTTGAATACATTGTGTTGGGCA
  ATAGGCTCCATTAGTGGAGCAATGCATGAAGAGGACGAAAAACGATTTCTTGTTACTGTT
  ATAAAGGATCTATTAGGATTATGTGAACAGAAAAGAGGCAAAGATAATAAAGCTATTATT
  GCATCAAATATCATGTACATAGTAGGTCAATACCCACGTTTTTTGAGAGCTCACTGGAAA
  TTTCTGAAGACTGTAGTTAACAAGCTGTTCGAATTCATGCATGAGACCCATGATGGAGTC
  CAGGATATGGCTTGTGATACTTTCATTAAAATAGCCCAAAAATGCCGCAGGCATTTCGTT
  CAGGTTCAGGTTGGAGAAGTGATGCCATTTATTGATGAAATTTTGAACAACATTAACACT
  ATTATTTGTGATCTTCAGCCTCAACAGGTTCATACGTTTTATGAAGCTGTGGGGTACATG
  ATTGGTGCACAAACAGATCAAACAGTACAAGAACACTTGATAGAAAAGTACATGTTACTC
  CCTAATCAAGTGTGGGATAGTATAATCCAGCAGGCAACCAAAAATGTGGATATACTGAAA
  GATCCTGAAACAGTCAAGCAGCTTGGTAGCATTTTGAAAACAAATGTGAGAGCCTGCAAA
  GCTGTTGGACACCCCTTTGTAATTCAGCTTGGAAGAATTTATTTAGATATGCTTAATGTA
  TACAAGTGCCTCAGTGAAAATATTTCTGCAGCTATCCAAGCTAATGGTGAAATGGTTACA
  AAGCAACCATTGATTAGAAGTATGCGAACTGTAAAAAGGGAAACTTTAAAGTTAATATCT
  GGTTGGGTGAGCCGATCCAATGATCCACAGATGGTCGCTGAAAATTTTGTTCCCCCTCTG
  TTGGATGCAGTTCTCATTGATTATCAGAGAAATGTCCCAGCTGCTAGAGAACCAGAAGTG
  CTTAGTACTATGGCCATAATTGTCAACAAGTTAGGGGGACATATAACAGCTGAAATACCT
  CAAATATTTGATGCTGTTTTTGAATGCACATTGAATATGATAAATAAGGACTTTGAAGAA
  TATCCTGAACATAGAACGAACTTTTTCTTACTACTTCAGGCTGTCAATTCTCATTGTTTC
  CCAGCATTCCTTGCTATTCCACCTACACAGTTTAAACTTGTTTTGGATTCCATCATTTGG
  GCTTTCAAACATACTATGAGGAATGTCGCAGATACGGGCTTACAGATACTTTTTACACTC
  TTACAAAATGTTGCACAAGAAGAAGCTGCAGCTCAGAGTTTTTATCAAACTTATTTTTGT
  GATATTCTCCAGCATATCTTTTCTGTTGTGACAGACACTTCACATACTGCTGGTTTAACA
  ATGCATGCATCAATTCTTGCATATATGTTTAATTTGGTTGAAGAAGGAAAAATAAGTACA
  TCATTAAATCCTGGAAATCCAGTTAACAACCAAATCTTTCTTCAGGAATATGTGGCTAAT
  CTCCTTAAGTCGGCCTTCCCTCACCTACAAGATGCTCAAGTAAAGCTCTTTGTGACAGGG
  CTTTTCAGCTTAAATCAAGATATTCCTGCTTTCAAGGAACATTTAAGAGATTTCCTAGTT
  CAAATAAAGGAATTTGCAGGTGAAGACACTTCTGATTTGTTTTTGGAAGAGAGAGAAATA
  GCCCTACGGCAGGCTGATGAAGAGAAACATAAACGTCAAATGTCTGTCCCTGGCATCTTT
  AATCCACATGAGATTCCAGAAGAAATGTGTGATTAA
  
  Clone variation with respect to NM_003400.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003400 unedited
  TATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGTAAATATTATTGAGCTCTTT
  TTGCCCTTTTCCTGTCCGTTTTTTTAATTTCCTTTTTTGAGGTGGGAAAACTGAAACCCA
  CCTTGATTCGTCCCCTCTCCCCCCTCCCCACCTTCCCTCGCCCTAATCCCCCAACGAGGA
  AGGAAGGAGCAGTTGGTTCAATCTCTGGTAATCTATGCCAGCAATTATGACAATGTTAGC
  AGACCATGCAGCTCGTCAGCTGCTTGATTTCAGCCAAAAACTGGATATCAACTTATTAGA
  TAATGTGGTGAATTGCTTATACCATGGAGAAGGAGCCCAGCAAAGAATGGCTCAAGAAGT
  ACTGACACATTTAAAGGAGCATCCTGATGCTTGGACAAGAGTCGACACAATTTTGGAATT
  TTCTCAGAATATGAATACGAAATACTATGGACTACAAATTTTGGAAAATGTGATAAAAAC
  AAGGTGGAAGATTCTTCCAAGGAACCAGTGCGAAGGAATAAAAAAATACGTTGTTGGCCT
  CATTATCAAGACGTCATCTGACCCAACTTGTGTAGAGAAAGAAAAGGTGTATATCGGAAA
  ATTAAATATGATCCTTGTTCAGATACTGAAACAAGAATGGCCCAAACATTGGCCAACTTT
  TATCAGTGATATTGTTGGAGCAAGTAGGACCAGCGAAAGTCTCTGTCAAAATATATGGTG
  ATTCTTAAACTCTTGAGTGAAGAAGTATTTGATTTCTCTAGTGGACAGATACCCCAGTCA
  NATCTAAGCATTTAAAGACAGCATGTGCCATGGATNCTCACAGATATTTCAACTGTGTCA
  GTTTGTATGGAAAATCTCAAATGCTCCACTTGACATGCCACCTTGGAACATGCTCAGATT
  CTGACCTGGATCCCTGGGATTATTTTTGAGACAAATAATCAGACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003400 unedited
  GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTAAATGTAAACCAAGTTTATTTTGCTTTTTAAGTAGGGTTCTTAAAGCACTACAGCTT
  GGTAAACAATGTAAATTAGTATAAGTCATCTCAAAAGCCAGAGTTGTTTTGTTTTTTAAT
  GAGTTGTTAAAAAGATGCAGCCTATTCTAACAGGATAAATATTTTTAACCATTTCACTTC
  GAGTTAATGAAGGCTCACAAATGAGCAACACTCCTCATTGAGGAAAACAAAAAGCTGTTG
  TCGACAAGCGACAGCCCACACCCCCAAAAACAAAAAGAACTGGGTAAAAATAACTGGGTT
  CCCATATTTTTGAAGTCGCTTTACAATGGGATGATATGCACATGATCTTGCTGCAATCTT
  GCCAAAGAGACTTTGTAATACATGTAGTCTAAACAAACGATTCAGTCCAAGAGGTGCTAA
  CTTCAGACAATGCAGCACTATAATCCTTTAAACAACGCAATTGGGTTTACTCATTATCTT
  TTCTTCTAGGCTGAAATAGACTAGAAGGAAAATGCTCCCTAATTAAAAATTGGTATTGTT
  TACAGGAAAAATTGTATAATTTTGCATTAGAATTACAAGTATATGTTCAAATCGAATTTG
  CCTCCTCCCCCCAGCCCAGCCACAAAAATGGGCATGAAGTAAAATTTTTAAAAATGCCTT
  AATTTTCAACTTCATGCAAACTAAATAAAGATGACCAAAACAAAAGCTTAAACAATGGAA
  GGATATTTCACAGAAATTTCTTATACAAAAAACACATAAGAAAAGGGCCACTAGGTGACA
  TTTTAATTACAAATTGGCTCATTTTTGTCGACAAAAACCACATGCTGTTTTTCTCTGCTA
  ACGAGTTGCAAAAAAAAAACGCCTGGATTTGATTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003400
• Insert Size: 4880 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003400.3, NP_003391.1
• RefSeq Size: 4830 bp
• RefSeq ORF: 3216 bp
• Locus ID: 7514
• Cytogenetics: 2p15
• Domains: IBN_NT
• Protein Families: Druggable Genome
• Gene Summary: 'This cell-cycle-regulated gene encodes a protein that mediates leucine-rich nuclear export signal (NES)-dependent protein transport. The protein specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. This protein also regulates NFAT and AP-1. [provided by RefSeq, Jan 2015]'