TXN (NM_003329) Human Untagged Clone

CAT#: SC118039

TXN (untagged)-Human thioredoxin (TXN)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TXN
• Synonyms: TRDX; TRX; TRX1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118039 sequence for NM_003329 edited (data generated by NextGen Sequencing)
  ATGGTGAAGCAGATCGAGAGCAAGACTGCTTTTCAGGAAGCCTTGGACGCTGCAGGTGAT
  AAACTTGTAGTAGTTGACTTCTCAGCCACGTGGTGTGGGCCTTGCAAAATGATCAAGCCT
  TTCTTTCATTCCCTCTCTGAAAAGTATTCCAACGTGATATTCCTTGAAGTAGATGTGGAT
  GACTGTCAGGATGTTGCTTCAGAGTGTGAAGTCAAATGCATGCCAACATTCCAGTTTTTT
  AAGAAGGGACAAAAGGTGGGTGAATTTTCTGGAGCCAATAAGGAAAAGCTTGAAGCCACC
  ATTAATGAATTAGTCTAA
  
  Clone variation with respect to NM_003329.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003329 unedited
  TACATTTTGTATACGACTCCTATAGGCGGCCGCGCAATTCGCACGAGGGTTTATAAAGGG
  AGAGAGCAAGCAGCGAGTCTTGAAGCTCTGTTTGGTGCTTTGGATCCATTTCCATCGGTC
  CTTACAGCCGCTCGTCAGACTCCAGCAGCCAAGATGGTGAAGCAGATCGAGAGCAAGACT
  GCTTTTCAGGAAGCCTTGGACGCTGCAGGTGATAAACTTGTAGTAGTTGACTTCTCAGCC
  ACGTGGTGTGGGCCTTGCAAAATGATCAAGCCTTTCTTTCATTCCCTCTCTGAAAAGTAT
  TCCAACGTGATATTCCTTGAAGTAGATGTGGATGACTGTCAGGATGTTGCTTCAGAGTGT
  GAAGTCAAATGCATGCCAACATTCCAGTTTTTTAAGAAGGGACAAAAGGTGGGTGAATTT
  TCTGGAGCCAATAAGGAAAAGCTTGAAGCCACCATTAATGAATTAGTCTAATCATGTTTT
  CTGAAAACATAACCAGCCATTGGCTATTTAAAACTTGTAATTTTTTTAATTTACAAAAAT
  ATAAAATATGAAGACATAAACCCAGTTGCCATCTGCGTGACAATAAAACATTAATGCTAA
  CACTTTNTTAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCCTG
  TTCCTGGACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGCCCCT
  GGAGTTGCCACTCAGTGCCCACCAGCCTGTCCTATAAAATAAATTGCATCATTTTGTCTG
  ACTAGGTGGCCTTCTATATATTATGGGGTGGAGGGGGCTGTATGGACCCAGGGCCAGTTG
  GGAAGACACCTGTAAGCCTGCGGGGTCTATTGGGACCAACTGGAATGGCATGCCCAAACT
  TGGCTACTGCATTTCCGCCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003329 unedited
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAAAATGTTAGCATTAAT
  GTTTTATTGTCACGCAGATGGCAACTGGGTTTATGTCTTCATATTTTATATTTTTGTAAA
  TTAAAAAAATTACAAGTTTTAAATAGCCAATGGCTGGTTATGTTTTCAGAAAACATGATT
  AGACTAATTCATTAATGGTGGCTTCAAGCTTTTCCTTATTGGCTCCAGAAAATTCACCCA
  CCTTTTGTCCCTTCTTAAAAAACTGGAATGTTGGCATGCATTTGACTTCACACTCTGAAG
  CAACATCCTGACAGTCATCCACATCTACTTCAAGGAATATCACGTTGGAATACTTTTCAG
  AGAGGGAATGAAAGAAAGGCTTGATCATTTTGCAAGGCCCACACCACGTGGCTGAGAAGT
  CAACTACTACAAGTTTATCACCTGCAGCGTCCAAGGCTTCCTGAAAAGCAGTCTTGCTCT
  CGATCTGCTTCACCATCTTGGCTGCTGGAGTCTGACGAGCGGCTGTAAGGACCGATGGAA
  ATGGATCCAAAGCACCAAACAGAGCTTCAAGACTCGCTGCTTGCTCTCTCCCTTTATAAA
  CCCTTGTGCCGAATTCGCGGTCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTC
  ACTAAACGAGCTCTGCTTATATAGACCTCCCACCTACACGCCTACCGCCCTTGTGCGTCA
  ACGGGGCGGGGTTTTTCCACCTTCTGGAACCCCCCTCGATTTTCGTGCCCCACCAACCCC
  CCTTGCCCCCAATGGGGGCGATACTTGGCAATCCCCTTGACCAAACCCCTCCCCCCCCCC
  TTTGTTTTTTCTGTTACCGTGTACCCTCGGCATAGGAAGACTAATCCTTCATGCACCTCC
  ATTTAGCAATCCCCCTAGGCTTTTTCTGGGATAACACCACTGGGTCTATCTATCATAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003329
• Insert Size: 630 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003329.2, NP_003320.2
• RefSeq Size: 508 bp
• RefSeq ORF: 318 bp
• Locus ID: 7295
• Cytogenetics: 9q31.3
• Domains: thiored
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).