TCTP (TPT1) (NM_003295) Human Untagged Clone

CAT#: SC118056

TPT1 (untagged)-Human tumor protein, translationally-controlled 1 (TPT1)


  "NM_003295" in other vectors (7)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol TPT1
Synonyms HRF; p02; p23; TCTP
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_003295, the custom clone sequence may differ by one or more nucleotides


ATGATTATCTACCGGGACCTCATCAGCCACGATGAGATGTTCTCCGACATCTACAAGATCCGGGAGATCG
CGGACGGGTTGTGCCTGGAGGTGGAGGGGAAGATGGTCAGTAGGACAGAAGGTAACATTGATGACTCGCT
CATTGGTGGAAATGCCTCCGCTGAAGGCCCCGAGGGCGAAGGTACCGAAAGCACAGTAATCACTGGTGTC
GATATTGTCATGAACCATCACCTGCAGGAAACAAGTTTCACAAAAGAAGCCTACAAGAAGTACATCAAAG
ATTACATGAAATCAATCAAAGGGAAACTTGAAGAACAGAGACCAGAAAGAGTAAAACCTTTTATGACAGG
GGCTGCAGAACAAATCAAGCACATCCTTGCTAATTTCAAAAACTACCAGTTCTTTATTGGTGAAAACATG
AATCCAGATGGCATGGTTGCTCTATTGGACTACCGTGAGGATGGTGTGACCCCATATATGATTTTCTTTA
AGGATGGTTTAGAAATGGAAAAATGTTAA


>OriGene 5' read for NM_003295 unedited
GGAATTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGGCACCATAGCCGCGCCCCGT
TTCCCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCACGCG
CCCTCCCGCGCGCGCGGGGCGCGTGGAGGGGGGGGGCGGCCCGCCGGCGGGGACAGGCGG
GGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGAGCTGCCGTATCGTTCCGCCTGGG
CGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTG
GCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCA
GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC
TAACCCCACTCCCCCCGAGCGCCGCTCCGGCTGCACCGCGCTCGCTCCGAGTTTCAGGCT
CGAGCTAAGCTAGCGCCGTCGTCGTCTCCCTTCAGTCGCCATCATGATTATCTACCGGGA
CCTCATCAGCCACGATGAGATGTTCTCCGACATCTACAAGATCCGGGAGATCGCGGACGG
GTTGTGCCTGGAGGTGGAGGGGAAGATGGTCAGTACGACAGAACGTAACATTGATGACTC
GCTCATTGGAGGAAATGCCTCCGCTGTATGCCCCGATGGCGAAAGTACCGAAAGCACAGT
AATCACTGGTGTCGATATTGCCATGAACCATCACCTGCAAGGAACAAGTCTCACATAAGA
AGCCTACAAGAAGTACATCAAAGATTACATGAAATCAATCAAAGGGAAACTCGAAGAACA
GAGACCAGGATAGATAATACCCTTTATGACAGGGGCTGCAGAACAAATCAGCACATCCTT
GCTAATTTCAAAACTACCAGTTCTCTATTGGTGGAAACATGAATTCCAAAGGCATGGCTG
CTCTATGCACTACCGTGAGAATGGCGGACCCATTATGATCTTCTTAAGAAGGTTAA
>OriGene 3' read for NM_003295 unedited
GAGAAAAGGAANAANANAAAAANTATTTNNNTNTCNNTTTTACTTTGNNCCGCGGNCGCG
CAACTAGNGATCGGTTTTTTTTTTTTTTTTTTTGAGTTTAAATGCATTTTATTTTTAGAC
AACCTACATGACATGTTTTTCTTAAAAACAATGCCTCCACTCCAAATAAATCACAGTCAA
AATAAATGAAGAGCTCAAGATGACATCAGTCCCATTTGTCTTAAGTCCTGGTGTTGTGTG
GATGACAAGCAGAAGCCAGTTATGATGACAGGTGATAGATCCAAAATAATTGCCACATTT
GTTAACATTTTTCCATTTCTAAACCATCCTTAAAGAAAATCATATATGGGGTCACACCAT
CCTCACGGTAGTCCAATAGAGCAACCATGCCATCTGGATTCATGTTTTCACCAATAAAGA
ACTGGTAGTTTTTGAAATTAGCAAGGATGTGCTTGATTTGTTCTGCAGCCCCTGTCATAA
AAGGTTTTACTCTTTCTGGTCTCTGTTCTTCAAGTTTCCCTTTGATTGATTTCATGTAAT
CTTTGATGTACTTCTTGTAGGCTTCTTTTGTGAAACTTGTTTCCTGCANGTGATGGTTCA
TGACAATATCGACACCAGTGATTACTGTGCTTTCGGTACCTTCGCCCTCGGGGCCTTCAG
CGGGAGCATTTCCCCAATGAGCGAGTCATCAATGTTACCTTCTGTCCTACTGACCATCTT
NCCCCTCCACCTTCAGGCACAACCCGTCCGCGATCTCCCGGATCTTGTAGAATGTCCGAG
ACATCTCATCGTGGCTGATGAAGTCCCCGCTAGATAATCATGATGGCCGACTGTAAGGGA
GCACCACGACGGGCGCTTAGCTTAACACGAGCCTTGAAAACTTGGAACGAGCGCCGTGCC
ACCCGGNACCGNGCCTCNGNGGGGAATGGGGTTAGACCCGCTCTGAAACAGGTTAAATTT
ACCCTACTGAAGAAGGGTTGGTGGCAGGG
Restriction Sites ECoRI-NOT     
ACCN NM_003295
Insert Size 1340 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003295.1, NP_003286.1
RefSeq Size 830 bp
RefSeq ORF 519 bp
Locus ID 7178
Cytogenetics 13q14.13
Domains TCTP
Gene Summary 'This gene encodes a protein that is a regulator of cellular growth and proliferation. Its mRNA is highly structured and contains an oligopyrimidine tract (5'-TOP) in its 5' untranslated region that functions to repress its translation under quiescent conditions. The encoded protein is involved in a variety of cellular pathways, including apoptosis, protein synthesis and cell division. It binds to and stabilizes microtubules, and removal of this protein through phosphorylation is required for progression through mitotic and meiotic cell divisions. This gene is known to play a role in carcinogenesis, and is upregulated in some cancer cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]'
Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a shorter C-terminus than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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