TCF7 (NM_003202) Human Untagged Clone

CAT#: SC118129

TCF7 (untagged)-Human transcription factor 7 (T-cell specific, HMG-box) (TCF7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCF7
• Synonyms: TCF-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003202, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCGCAGCTGGACTCCGGCGGGGGCGGCGCGGGCGGCGGCGACGACCTCGGCGCGCCGGACGAGCTGC
  TGGCCTTCCAGGATGAAGGCGAGGAGCAGGACGACAAGAGCCGCGACAGCGCCGCCGGTCCCGAGCGCGA
  CCTGGCCGAGCTCAAGTCGTCGCTCGTGAACGAGTCCGAGGGCGCGGCCGGCGGCGCAGGGATCCCGGGG
  GTCCCGGGGGCCGGCGCCGGGGCCCGCGGCGAGGCCGAGGCTCTCGGGCGGGAACACGCTGCGCAGAGAC
  TCTTCCCGGACAAACTTCCAGAGCCCCTGGAGGACGGCCTGAAGGCCCCGGAGTGCACCAGCGGCATGTA
  CAAAGAGACCGTCTACTCCGCCTTCAATCTGCTCATGCATTACCCACCCCCCTCGGGAGCAGGGCAGCAC
  CCCCAGCCGCAGCCCCCGCTGCACAAGGCCAATCAGCCCCCCCACGGTGTCCCCCAACTCTCTCTCTACG
  AACATTTCAACAGCCCACATCCCACCCCTGCACCTGCGGACATCAGCCAGAAGCAAGTTCACAGGCCTCT
  GCAGACCCCTGACCTCTCTGGCTTCTACTCCCTGACCTCAGGCAGCATGGGGCAGCTCCCCCACACTGTG
  AGCTGGTTCACCCACCCATCCTTGATGCTAGGTTCTGGTGTACCTGGTCACCCAGCAGCCATCCCCCACC
  CGGCCATTGTGCCCCCCTCAGGGAAGCAGGAGCTGCAGCCCTTCGACCGCAACCTGAAGACACAAGCAGA
  GTCCAAGGCAGAGAAGGAGGCCAAGAAGCCAACCATCAAGAAGCCCCTCAATGCCTTCATGCTGTACATG
  AAGGAGATGAGAGCCAAGGTCATTGCAGAGTGCACACTTAAGGAGAGCGCTGCCATCAACCAGATCCTGG
  GCCGCAGGTGGCACGCGCTGTCGCGAGAAGAGCAGGCCAAGTACTATGAGCTGGCCCGCAAGGAGAGGCA
  GCTGCACATGCAGCTATACCCAGGCTGGTCAGCGCGGGACAACTACGGGAAGAAGAAGAGGCGGTCGAGG
  GAAAAGCACCAAGAATCCACCACAGGAGGAAAAAGAAATGCATTCGGTACTTACCCGGAGAAGGCCGCTG
  CCCCAGCCCCGTTCCTTCCGATGACAGTGCTCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003202 unedited
  GCAGATATTTGTATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGGCCGGACGA
  GCTGCTGGCCTTCCAGGATGAAGGCGAGGAGCAGGACGACAAGAGCCGCGACAGCGCCGC
  CGGTCCCGAGCGCGACCTGGCCGAGCTCAAGTCGTCGCTCGTGAACGAGTCCGAGGGCGC
  GGCCGGCGGCGCAGGGATCCCGGGGGTCCCGGGGGCCGGCGCCGGGGCCCGCGGCGAGGC
  CGAGGCTCTCGGGCGGGAACACGCTGCGCAGAGACTCTTCCCGGACAAACTTCCAGAGCC
  CCTGGAGGACGGCCTGAAGGCCCCGGAGTGCACCAGCGGCATGTACAAAGAGACCGTCTA
  CTCCGCCTTCAATCTGCTCATGCTTTACCCACCCCCCTCGGGAGCAGGGCAGCACCCCCA
  GCCGCAGCCCCCGCTGCACAAGGCCAATCAGCCCCCCCACGGTGTCCCCCAACTCTCTCT
  CTACGAACATTTCAACAGCCCACATCCCACCCCTGCACCTGCGGACATCAGCCAGAAGCA
  AGTTCACAGGCCTCTGCAGACCCCTGACCTCTCTGGCTTCTACTCCCTGACCTCAGGCAG
  CATGGGGCAGCTCCCCCACACTGTGAGCTGGTTCACCCACCCATCCTTGATGCTAGGGTT
  CTGGTGTACCTGGTCACCCAGCAGCCATCCCCCACCCGGCCATTGTGCCCCCCTCNAGGA
  AGCAGGAGCTGCAGCCCTTCGACCGCAACCTGAAGAACAAGCAGAGTCCAAGGCAGAGAA
  GGAGGCCAAGAAGCCAACCATCAAGAAGCCCCTCAATGCCTTCATGCTGNTACATGANAG
  GAGATGAGAGCCAAGGTCATTGTCAGAGTGCACACTTNANGGAGAGCGCTGCCATCACCA
  GATCTGGNNGCCGCAGGTGGCACGCGCTGTCGCAGNAAAACAGGCCAAGTCTTATGAGCT
  GGCCGCCAAGGAGAGCAGCTGACAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003202 unedited
  TGTACGCGGCCGCAATCTANAGTCGAGNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTCAAGGTTTTAAAAAAACAACCCCACTAAAAAGT
  TTTTTTTTTCTAAGGTTAAAAAGTTTTTTTATGAGGAGGCTTTACCAAACCAAAAATTTT
  TGGAACCCTAAAAAAATTTTTTTCTAAAAACTTTTGGCGGGGTAACACTAAAAAAGGGGA
  CTTTGGGATTCGGAAAAACCAGGGGTTAAAGGAGGACCTAAGGGTTTTTTTGGGGGGGGG
  GGGAAAACCAGGGACAGCCCTTTTTTTTTTTTTTTTTTTTTTTGGAAAGGCACCCCCCTT
  GTCCCCCAAACGGGGGGGCAAAACCCCCTTCTGGGCTAATGGGAACCCCCCCCTCCGGGG
  TTAAAGGGATTTTCCCCCCCCACCCCCCCGAGTAGGGGGGATTACAGGGCCCCCCCACCA
  GGGCGGGTTAATTTTTGTTTTTTAAGAAAAACCAGGGTTTCCCCAGGGGGGCCAGGTGGG
  CTCAAAACTTCGGACGCGGGGGGACCCCCCCGCCCCGGCCCGCAAAAGGGCTGGGATCAC
  GGGCGGGAGACCCCGGCCCGGGGCCAAAAGCCTTTTTTTTTTGGGTTTAGGCAAAAAACA
  AAATTAAATTAAGGATTTGTTTTTAAAGGTTGCGGGTTGAACCTGGGTTCAAAAAGGGGT
  AACCGGAGCCTTTGGGGAAACCCGGGTGCCCATTCCGGAGGGTTTTGGGGCCTCTCTGAA
  TATTGAAAGGCGCTTCTGCCCCCCCCCCTTGGGGAAAATGGACCCGCCCGGGGGCTGAAG
  GCTAAAAAAAGCCTTGGGCTTCAAGAGAGGTTTGGTTTGCCCTCAACACGCCCCAAGACT
  GGGGGAATGCCCAAAAGAG
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_003202
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003202.1, NP_003193.1
• RefSeq Size: 3277 bp
• RefSeq ORF: 807 bp
• Locus ID: 6932
• Cytogenetics: 5q31.1
• Domains: HMG
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Melanogenesis, Pathways in cancer, Prostate cancer, Thyroid cancer, Wnt signaling pathway
• Gene Summary: 'This gene encodes a member of the T-cell factor/lymphoid enhancer-binding factor family of high mobility group (HMG) box transcriptional activators. This gene is expressed predominantly in T-cells and plays a critical role in natural killer cell and innate lymphoid cell development. The encoded protein forms a complex with beta-catenin and activates transcription through a Wnt/beta-catenin signaling pathway. Mice with a knockout of this gene are viable and fertile, but display a block in T-lymphocyte differentiation. Alternative splicing results in multiple transcript variants. Naturally-occurring isoforms lacking the N-terminal beta-catenin interaction domain may act as dominant negative regulators of Wnt signaling. [provided by RefSeq, Oct 2016]'
  Transcript Variant: This variant (1) encodes isoform (1).