Thymine DNA glycosylase (TDG) (NM_003211) Human Untagged Clone

CAT#: SC118135

TDG (untagged)-Human thymine-DNA glycosylase (TDG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TDG
• Synonyms: hTDG
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118135 sequence for NM_003211 edited (data generated by NextGen Sequencing)
  ATGGAAGCGGAGAACGCGGGCAGCTATTCCCTTCAGCAAGCTCAAGCTTTTTATACGTTT
  CCATTTCAACAACTGATGGCTGAAGCTCCTAATATGGCAGTTGTGAATGAACAGCAAATG
  CCAGAAGAAGTTCCAGCCCCAGCTCCTGCTCAGGAACCAGTGCAAGAGGCTCCAAAAGGA
  AGAAAAAGAAAACCCAGAACAACAGAACCAAAACAACCAGTGGAACCCAAAAAACCTGTT
  GAGTCAAAAAAATCTGGCAAGTCTGCAAAATCAAAAGAAAAACAAGAAAAAATTACAGAC
  ACATTTAAAGTAAAAAGAAAAGTAGACCGTTTTAATGGTGTTTCAGAAGCTGAACTTCTG
  ACCAAGACTCTCCCCGATATTTTGACCTTCAATCTGGACATTGTCATTATTGGCATAAAC
  CCGGGACTAATGGCTGCTTACAAAGGGCATCATTACCCTGGACCTGGAAACCATTTTTGG
  AAGTGTTTGTTTATGTCAGGGCTCAGTGAGGTCCAGCTGAACCATATGGATGATCACACT
  CTACCAGGGAAGTATGGTATTGGATTTACCAACATGGTGGAAAGGACCACGCCCGGCAGC
  AAAGATCTCTCCAGTAAAGAATTTCGTGAAGGAGGACGTATTCTAGTACAGAAATTACAG
  AAATATCAGCCACGAATAGCAGTGTTTAATGGAAAATGTATTTATGAAATTTTTAGTAAA
  GAAGTTTTTGGAGTAAAGGTTAAGAACTTGGAATTTGGGCTTCAGCCCCATAAGATTCCA
  GACACAGAAACTCTCTGCTATGTTATGCCATCATCCAGTGCAAGATGTGCTCAGTTTCCT
  CGAGCCCAAGACAAAGTTCATTACTACATAAAACTGAAGGACTTAAGAGATCAGTTGAAA
  GGCATTGAACGAAATATGGACGTTCAAGAGGTGCAATATACATTTGACCTACAGCTTGCC
  CAAGAGGATGCAAAGAAGATGGCTGTTAAGGAAGAAAAATATGATCCAGGTTATGAGGCA
  GCATATGGTGGTGCTTACGGAGAAAATCCATGCAGCAGTGAACCTTGTGGCTTCTCTTCA
  AATGGGCTAATTGAGAGCGTGGAGTTAAGAGGAGAATCAGCTTTCAGTGGCATTCCTAAT
  GGGCAGTGGATGACCCAGTCATTTACAGACCAAATTCCTTCCTTTAGTAATCACTGTGGA
  ACACAAGAACAGGAAGAAGAAAGCCATGCTTAA
  
  Clone variation with respect to NM_003211.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003211 unedited
  GTTTTGTATACGACTCATATAGGCGGCCGCGACATTCGCACGAGGGGAGCTTGAGTCCAG
  CCACTGTCTGGGTACTGCCAGCCATCGGGCCCAGGTCTCTGGGGTTGTCTTACCGCAGTG
  AGTACCACGCGGTACTACAGAGACCGGCTGCCCGTGTGCCCGGCAGGTGGAGCCGCCCGC
  ATCAGCGGCCTCGGGGAATGGAAGCGGAGAACGCGGGCAGCTATTCCCTTCAGCAAGCTC
  AAGCTTTTTATACGTTTCCATTTCAACAACTGATGGCTGAAGCTCCTAATATGGCAGTTG
  TGAATGAACAGCAAATGCCAGAAGAAGTTCCAGCCCCAGCTCCTGCTCAGGAACCAGTGC
  AAGAGGCTCCAAAAGGAAGAAAAAGAAAACCCAGAACAACAGAACCAAAACAACCAGTGG
  AACCCAAAAAACCTGTTGAGTCAAAAAAATCTGGCAAGTCTGCAAAATCAAAAGAAAAAC
  AAGAAAAAATTACAGACACATTTAAAGTAAAAAGAAAAGTAGACCGTTTTAATGGGTGTT
  TCAGAAGCTGAACTTCTGACCAAGACTCTCCCCGATATTTTGACCTTCAATCTGGACATT
  GTCATTATTGGCATAAACCCGGGACTAATGGCTGCTTACAAGGGCATCATTACCCTGGAC
  CTGGAACCATTTTTGGAAGTGTTTGTTTATGTCAGGGCTAANNGGAGGTNCAGCTGACCA
  TTGGGATGATCACACTTACAAGGAANGTATTGGTATTTGGATTTACAACATGGTGGAAAG
  GGACCACGCCGGCGCCAAGATCTCTCAGTAAGAATTTCTGAAGGAGGGACGTTTTTTGTT
  CAGAAATACAGATATCGCCGAATANCAGGTTATGGGAAATGGTTTATGAATTTTNNNNAA
  AAATTTTTGGGGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003211 unedited
  CCCCCCCCATTCTTGNACGNGGNCATGTATTTATCNGATTTTTTTTTTTTTTTGAGAGGG
  ATCTTGCTCTGTTACCCAGCTGGAGTGCAGGTGTAAATGGGGAAAATGCGNACTTTGAAA
  GGAATTTAAAACTTTCAATATATTTAAGAAAAGGTTCAACTGTCATGTATAGGAATAACC
  GCCCTGGGGCAAAAAATTTTTTATTTTTTTTATAAAAAAGGGGGGTTTACTTTCGCTGGG
  AGATCTCTAGGGGAGGTCCGTCATAATTTAAGGGGTATTTTCCCCATTCAAAAAAAACAA
  TTTTTTGTAAAGCTACAATAGTTATATACCAAAGCAATACCTAGTTACATGCTTTACACA
  GTCCCGGGAAAAAAAAATTTAATTGCTCCTAATCCCTGAGGCAGGGCATTTTAAAGCACC
  CGCACAAAAGGTCCATGTAAACAGCAGTACAGTACATTATTTAAATAACATAAAGGACTT
  TTACACAGTTTGACCTAGGAAAAAATAAAATCCATTATAGCCACAGTTAAAAAGCATGTT
  AAGATTCACAATAAGAATTTGTTTTTTTTATTATAAAGAGAAGAGCAATCATATAACCTC
  CTGGGGGGGGGGGAGACCTCATAAATATTTTATATTGATTGACAAAACAGCATGCTCCCT
  GGGTACGTATGAAATCAGAATATTCAGGAGTAACTGAGGAGGATGCAAATAACATTTTTT
  AGTACCCAGCTTTAATATCTACCAGACCTGGTAGTCTGGATTATTTGTAGCACCATGGAA
  AACCATTAGAAAATCACAAGGTATACACCTCAACCTTTCCATGAGGGTCATTTAATTTTA
  TGTAACAAACAGCCATGAGC
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_003211
• Insert Size: 3570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003211.3, NP_003202.3
• RefSeq Size: 3229 bp
• RefSeq ORF: 1233 bp
• Locus ID: 6996
• Cytogenetics: 12q23.3
• Domains: UDG
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• Gene Summary: 'The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008]'