SMARCE1 (NM_003079) Human Untagged Clone

CAT#: SC118214

SMARCE1 (untagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (SMARCE1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMARCE1
• Synonyms: BAF57; CSS5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003079 edited
  GAATTCGGCACGAGGCTCAGATTCATTCTTAAGGAACTGAGAACTTAATCTTCCAAAATG
  TCAAAAAGACCATCTTATGCCCCACCTCCCACCCCAGCTCCTGCAACACAAATGCCCAGC
  ACACCAGGGTTTGTGGGATACAATCCATACAGTCATCTCGCCTACAACAACTACAGGCTG
  GGAGGGAACCCGGGCACCAACAGCCGGGTCACGGCATCCTCTGGTATCACGATTCCAAAA
  CCCCCAAAGCCACCAGATAAGCCGCTGATGCCCTACATGAGGTACAGCAGAAAGGTCTGG
  GACCAAGTAAAGGCTTCCAACCCTGACCTAAAGTTGTGGGAGATTGGCAAGATTATTGGT
  GGCATGTGGCGAGATCTCACTGATGAAGAAAAACAAGAATATTTAAACGAATACGAAGCA
  GAAAAGATAGAGTACAATGAATCTATGAAGGCCTATCATAATTCCCCCGCGTACCTTGCT
  TACATAAATGCAAAAAGTCGTGCAGAAGCTGCTTTAGAGGAAGAAAGTCGACAGAGACAA
  TCTCGCATGGAGAAAGGAGAACCGTACATGAGCATTCAGCCTGCTGAAGATCCAGATGAT
  TATGATGATGGCTTTTCAATGAAGCATACAGCCACCGCCCGTTTCCAGAGAAACCACCGC
  CTCATCAGTGAAATTCTTAGTGAGAGTGTGGTGCCAGACGTTCGGTCAGTTGTCACAACA
  GCTAGAATGCAGGTCCTCAAACGGCAGGTCCAGTCCTTAATGGTTCATCAGCGAAAACTA
  GAAGCTGAACTTCTTCAAATAGAGGAACGACACCAGGAGAAGAAGAGGAAATTCCTGGAA
  AGCACAGATTCATTTAACAATGAACTTAAAAGGTTGTGCGGTCTGAAAGTAGAAGTGGAT
  ATGGAGAAAATTGCAGCTGAGATTGCACAGGCAGAGGAACAGGCCCGCAAAAGGCAGGAG
  GAAAGGGAGAAGGAGGCCGCAGAGCAAGCTGAGCGCAGTCAGAGCAGCATCGTTCCTGAG
  GAAGAACAAGCAGCTAACAAAGGCGAGGAGAAGAAAGACGACGAGAACATTCCGATGGAG
  ACAGAGGAGACACACCTTGAAGAAACAACAGAGAGCCAACAGAATGGTGAAGAAGGCACG
  TCTACTCCTGAGGACAAGGAGAGTGGGCAGGAGGGGGTCGACAGTATGGCAGAGGAAGGA
  ACCAGTGATAGTAACACTGGCTCGGAGAGCAACAGTGCAACAGTGGAGGAGCCACCAACA
  GATCCCATACCAGAAGATGAGAAAAAAGAATAAGTGTTGCCTTGTTTTGTGTGTTCTAAA
  TACTTTTTTTAATGAAAAAATGTTTTTTGGTTTTAATGGTGTTACGTGGTTTGTGTATTA
  ATTTTTTTTCTTGTCCATATCATACCACCAAAGGCTTTTGGACCATTTAGCATCATGAGC
  CTAATGGCTCAGTCAGTCACCTTTCTTAAGTGTTGTGAAGATGGCTCTTTTCTTTGGATC
  TTGTTTCTAGCCCTCAACTGCTGAAAGCCTCAGAATTTAGATTAATTGAGAAAACACCCA
  CCTCTTTTAGAGAATTATCCTTTGATGCTGCAGAATCTACTCTTACAATGCCTTCCTACA
  GCTCACTGGGGTGCTTACCAAAGCCATAGCTTTAAACCTTCCCAGTCCCCATCAGTAGCT
  TCCTGAAAGTCTCCTCTCTTGTTTACTTCTGCAAAGGGTAGCTTCTTAAAAACGTGATCA
  TGTATGAGTATGTATTTGTTCACTTACCCTTTTTTACTTTTAXXXXXXXXXXXXXXXXXX
  XXXXXXXXXXXXAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCG
  AC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003079 unedited
  GGTTTGCANATTTTGTATACGATTCCTATAGGCGGCCCGCGAATTCGCACGAGGCTCAGA
  TTCATTCTTAAGGAACTGAGAACTTAATCTTCCAAAATGTCAAAAAGACCATCTTATGCC
  CCACCTCCCACCCCAGCTCCTGCAACACAAATGCCCAGCACACCAGGGTTTGTGGGATAC
  AATCCATACAGTCATCTCGCCTACAACAACTACAGGCTGGGAGGGAACCCGGGCACCAAC
  AGCCGGGTCACGGCATCCTCTGGTATCACGATTCCAAAACCCCCAAAGCCACCAGATAAG
  CCGCTGATGCCCTACATGAGGTACAGCAGAAAGGTCTGGGACCAAGTAAAGGCTTCCAAC
  CCTGACCTAAAGTTGTGGGAGATTGGCAAGATTATTGGTGGCATGTGGCGAGATCTCACT
  GATGAAGAAAAACAAGAATATTTAAACGAATACGAAGCAGAAAAGATAGAGTACAATGAA
  TCTATGAAGGCCTATCATAATTCCCCCGCGTACCTTGCTTACATAAATGCAAAAAGTCGT
  GCAGAAGCTGCTTTAGAGGAAGAAAGTCGACAGAGACAATCTCGCATGGAGAAAGGAGAA
  CCGTACATGAGCATTCAGCCTGCTGAAGATCCAGATGATTATGATGATGCCTTTTCAATG
  AAGCATACAGCCACCGCCCGTTTCCAGAAGAACCACCGCCTCATCAGTGAAATTCTTAGT
  GAGAGTGTGGTGCCAGACGTTCGGTCAGTTGTCACACCAGCTAGAATGCANGTTCTCAAA
  CGGGCAGTCCCAGTCTTAATGGGTCATCAGCGAAAACCTAAAGCTGAACTTCTCCAATAG
  AGGCACGACCCCAGNNAGAGAAGAGAAATTCCTCGGAAGCCCANAATCTTTTAACATGAA
  CTTAAAAGTTGTGCGGTCTGAAAGTACATTGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003079 unedited
  GCTGGGGTTACTTTGGTCCTTTAANAACATATACCATCAGNAATACCACTATTCAGTAAA
  AATCTATTCTAGTTTTTGGGAAGCTTATGTGTCACAAACTATCTTGCTTCTAGTCTCCAA
  GACTTTACTAAGTTTATCAAAAAGGTGGGCTCTGGTCTATGGGATCCTGAGCCTTGAGGT
  TTGGATGTTCTGATTATTGTAAAACAATTTCAAGTGGATTAACTTAATTCCCAGCTGTTT
  TTCTTCTGGAATGGGCTAATCAGTTTGAATTATCTAGTCCATTACAAGAATATTAAATTC
  CTTGAGCTTTGTGATCTATTCTGTCTTTGGAAAACCTGGTAAAAAAGTAATTTTTTTTCT
  ATACAATAAGGATGGATTTACCAGAACATACCCAGTATTAATCATAACAAAAAGCAGTAT
  AGATAATGTATATGTGCTGTGTTCACTTTAATAACCACTTCATAAGTAACTACTCTAACT
  ATGGGGACTATTTCTGGATCAGTAAGATCCAAGTGTAGTTAGTTACACACTACACTCAGC
  TTAACACAACTCTTGGTATCTGACATTGATTAAAAGTAAAAAAGGGTAAGTGAACAAATA
  CATACTCATACATGATCACGTTTTTAAGAAGCTACCCTTTGCAGAAGTAAACAAGAGAGG
  AGACTTTCAGGAAGCTACTGATGGGGACTGGGAAGGGTTAAAGCTATGGCTNTGGTAAGC
  ACCCCAGTGAGCTGTAGGAAGGCATTGTAAGAGTAGATTCTGCAGCATCAAAGGATAATT
  CTCCTAAAGAAGTGGGTGTTTTCTCAATTAATCTAAATTCTGAGGCTTTCAGCAGTTTGA
  GGCTANNAACANGATCCAAAGAAAAAAGCCTCCTCCCACCACTTAAGAAAGTGACTGACT
  GACCATTAGCTCATGAGCTAAAAGTCCAAAAGCCTTTGTTGTATGAATAGACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003079
• Insert Size: 2550 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003079.4, NP_003070.3
• RefSeq Size: 2425 bp
• RefSeq ORF: 1236 bp
• Locus ID: 6605
• Cytogenetics: 17q21.2
• Domains: HMG
• Protein Families: Transcription Factors
• Gene Summary: 'The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq, Jul 2008]'