CD162 (SELPLG) (NM_003006) Human Untagged Clone

CAT#: SC118237

SELPLG (untagged)-Human selectin P ligand (SELPLG), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SELPLG
• Synonyms: CD162; CLA; PSGL-1; PSGL1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003006, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCTCTGCAACTCCTCCTGTTGCTGATCCTACTGGGCCCTGGCAACAGCTTGCAGCTGTGGGACACCT
  GGGCAGATGAAGCCGAGAAAGCCTTGGGTCCCCTGCTTGCCCGGGACCGGAGACAGGCCACCGAATATGA
  GTACCTAGATTATGATTTCCTGCCAGAAACGGAGCCTCCAGAAATGCTGAGGAACAGCACTGACACCACT
  CCTCTGACTGGGCCTGGAACCCCTGAGTCTACCACTGTGGAGCCTGCTGCAAGGCGTTCTACTGGCCTGG
  ATGCAGGAGGGGCAGTCACAGAGCTGACCACGGAGCTGGCCAACATGGGGAACCTGTCCACGGATTCAGC
  AGCTATGGAGATACAGACCACTCAACCAGCAGCCACGGAGGCACAGACCACTCAACCAGTGCCCACGGAG
  GCACAGACCACTCCACTGGCAGCCACAGAGGCACAGACAACTCGACTGACGGCCACGGAGGCACAGACCA
  CTCCACTGGCAGCCACAGAGGCACAGACCACTCCACCAGCAGCCACGGAAGCACAGACCACTCAACCCAC
  AGGCCTGGAGGCACAGACCACTGCACCAGCAGCCATGGAGGCACAGACCACTGCACCAGCAGCCATGGAA
  GCACAGACCACTCCACCAGCAGCCATGGAGGCACAGACCACTCAAACCACAGCCATGGAGGCACAGACCA
  CTGCACCAGAAGCCACGGAGGCACAGACCACTCAACCCACAGCCACGGAGGCACAGACCACTCCACTGGC
  AGCCATGGAGGCCCTGTCCACAGAACCCAGTGCCACAGAGGCCCTGTCCATGGAACCTACTACCAAAAGA
  GGTCTGTTCATACCCTTTTCTGTGTCCTCTGTTACTCACAAGGGCATTCCCATGGCAGCCAGCAATTTGT
  CCGTCAACTACCCAGTGGGGGCCCCAGACCACATCTCTGTGAAGCAGTGCCTGCTGGCCATCCTAATCTT
  GGCGCTGGTGGCCACTATCTTCTTCGTGTGCACTGTGGTGCTGGCGGTCCGCCTCTCCCGCAAGGGCCAC
  ATGTACCCCGTGCGTAATTACTCCCCCACCGAGATGGTCTGCATCTCATCCCTGTTGCCTGATGGGGGTG
  AGGGGCCCTCTGCCACAGCCAATGGGGGCCTGTCCAAGGCCAAGAGCCCGGGCCTGACGCCAGAGCCCAG
  GGAGGACCGTGAGGGGGATGACCTCACCCTGCACAGCTTCCTCCCTTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003006 unedited
  GCGTCAGGATTTGTAATCACGACTTACTATAGGGNNCGGCCGCGCAATTCGGCACCGAGG
  GGACCTTGTCACTAAAGCAGAGAAGCCACTTCTTCTGGGCCCACGAGGCAGCTGTCCCAT
  GCTCTGCTGAGCACGGTGGTGCCATGCCTCTGCAACTCCTCCTGTTGCTGATCCTACTGG
  GCCCTGGCAACAGCTTGCAGCTGTGGGACACCTGGGCAGATGATATCCGAGAAAGCCTTG
  GGTCCCCTGCTTGCCCGGGACCGGAGACAGGCCACCGAATATGAGTACCTAGATTATGAT
  TTCCTGCCAGAAACGGAGCCTCCAGAAATGCTGAGGAACAGCACTGACACCACTCCTCTG
  ACTGGGCCTGGAACCCCTGAGTCTACCACTGTGGAGCCTGCTGCAAGGCGTTCTACTGGC
  CTGGATGCAGGAGGGGCAGTCACAGAGCTGACCACGGAGCTGGCCAACATGGGGAACCTG
  TCCACGGATTCAGCAGCTATGGAGATACAGACCACTCAACCAGCAGCCACGGAGGCACAG
  ACCACTCAACCAGTGCCCACGGAGGCACAGACCACTCCACTGGCAGCCACAGAGGCACAG
  ACAACTCGACTGACGGCCACGGAGGCACAGACCACTCCACTGGCAGCCACAGAGGCACAG
  ACCACTCCACCAGCAGCCACGGAAGCACAGACCACTCAACCCACAGGCCTGGAGGCACAG
  ACCACTGCACCAGCAGCCATGGAGGCACAGACCACTGCACCAGCAGCCATGGAAGCACAG
  ACCACTCCACCAGCAGCCATGGAGGCACAGACCACCTCAACCACAGCCATGGAGGCACAG
  ACCACTGCACCAGAAGCCACCGAGGCACAGACCAACTCACCCACAGCCACGGAGGCACGA
  CCACTCCACTGGCAGCCATGGAGGCCCTGTCCACAGACCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003006 unedited
  ACCGAAAGCCGCNTTCGATAGTCGAGTTTTTTTCTTTTTTTTTTGTCCATCACATATTTA
  TTGCCTGCACTGGACCTGCAGTTGGGATACTGCAGTGAACAAGACAGAGTCCACCCTGGA
  AAGTGGCCACAGGGGAAGGCAAGAGGCGATAGCTGGGGGTCACTTGTCACTAGATGGAAG
  CTCCTTGGTCCACACAGTCCCAAAGAAAGGTCTTTCCTTGGGCCCACGAAACCCATCCCA
  GCCCTCACAGGCTGAACCTCACCCTGGGTTTTCCCAGAGAGGTGCCCAAGAAGACCCAAG
  CTGCCCCAAGTCAGAGGAGCAGCAGGAAACAGCCTCAGAAGTCCGTCACTCTTCAGTACT
  CCTCCCTTAGAATGTCCACTTCCTGTTTGGTGACCATGGCAACCAAAAGACAATGGCAGT
  GCAGTGCGTGTGGGATGGGGGAGCTCCATCTTTCTTGGGTACATGGGGAGGAGACTCCAG
  TGACCAGGAGAAGCGGGGAGAGCCATGGGAGATGTTACAGGGACCCAGAGAAGATGGGGG
  ACAGGAAAAAGGAGAGAATTTGCCTCAAGTAAACGGCCTACTGCCTTGGACCTCGGATGA
  AACGTGGCTGGGCTTCATCCGCGGAGGGAGGAAAGAGGTGGCTCCACTTGCCCGCCCGCT
  TGGCCCAAAGCTGCTCTTGACCCGTTTGGGTGGCCACAGGTTCTACCCTGAAAATCCCAT
  CCCCAGGGGCTCCGAGCAACCCCACAGCCGCCGAATGTGGACCCGGGCACTCAGGGGTGG
  CCCAGGAGAGCCCGTGTAACTGGGGCCCTTGCCACAACAAATGGCTTAGCGAGCTAAAGG
  CAGGAAACTGTGGCAGGCGGAGGCCATATCCTCTCCGGGATCTCCTTGGGCCTCTGGNGA
  CAAGCCCTCGGCTTTTTGGCACTTGGGAAAGGCCACCCTATTGCCTGTAG
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_003006
• Insert Size: 2170 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003006.3, NP_002997.1
• RefSeq Size: 2550 bp
• RefSeq ORF: 1209 bp
• Locus ID: 6404
• Cytogenetics: 12q24.11
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: 'This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]'
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.