SFRS3 (SRSF3) (NM_003017) Human Untagged Clone

CAT#: SC118249

SRSF3 (untagged)-Human serine/arginine-rich splicing factor 3 (SRSF3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SRSF3
• Synonyms: SFRS3; SRp20
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118249 sequence for NM_003017 edited (data generated by NextGen Sequencing)
  ATGCATCGTGATTCCTGTCCATTGGACTGTAAGGTTTATGTAGGCAATCTTGGAAACAAT
  GGCAACAAGACGGAATTGGAACGGGCTTTTGGCTACTATGGACCACTCCGAAGTGTGTGG
  GTTGCTAGAAACCCACCCGGCTTTGCTTTTGTTGAATTTGAAGATCCCCGAGATGCAGCT
  GATGCAGTCCGAGAGCTAGATGGAAGAACACTATGTGGCTGCCGTGTAAGAGTGGAACTG
  TCGAATGGTGAAAAAAGAAGTAGAAATCGTGGCCCACCTCCCTCTTGGGGTCGTCGCCCT
  CGAGATGATTATCGTAGGAGGAGTCCTCCACCTCGTCGCAGATCTCCAAGAAGGAGAAGC
  TTCTCTCGCAGCCGGAGCAGGTCCCTTTCTAGAGATAGGAGAAGAGAGAGATCGCTGTCT
  CGGGAGAGAAATCACAAGCCGTCCCGATCCTTCTCTAGGTCTCGTAGTCGATCTAGGTCA
  AATGAAAGGAAATAG
  
  Clone variation with respect to NM_003017.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003017 unedited
  CGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGAGAGAGAGTTGGTTGGTGTTGGGC
  CGGAGGAAAGCGGGAAGACTCATCGGAGCGTGTGGATTTGAGCCGCCGCATTTTTTAACC
  CTAGATCTCGAAATGCATCGTGATTCCTGTCCATTGGACTGTAAGGTTTATGTAGGCAAT
  CTTGGAAACAATGGCAACAAGACGGAATTGGAACGGGCTTTTGGCTACTATGGACCACTC
  CGAAGTGTGTGGGTTGCTAGAAACCCACCCGGCTTTGCTTTTGTTGAATTTGAAGATCCC
  CGAGATGCAGCTGATGCAGTCCGAGAGCTAGATGGAAGAACACTATGTGGCTGCCGTGTA
  AGAGTGGAACTGTCGAATGGTGAAAAAAGAAGTAGAAATCGTGGCCCACCTCCCTCTTGG
  GGTCGTCGCCCTCGAGATGATTATCGTAGGAGGAGTCCTCCACCTCGTCGCAGATCTCCA
  AGAAGGAGAAGCTTCTCTCGCAGCCGGAGCAGGTCCCTTTCTAGAGATAGGAGAAGAGAG
  AGATCGCTGTCTCGGGAGAGAAATACAAGCCGTCCCGATCCTTCTCTAGGTCTCGTAGTC
  GATCTAGGTCAAATGAAAGGAAATAGAAGACAGTTTGCAAGAGAAGTGGTGTACAGGAAA
  TTACTTCATTTGACAGGAGTATGTACAGAAATTCAAGTTTTGTTTGAGACTTCATAGCTG
  GTGCATTTTAAGATGTTTAGCTGTCAAATCTGTTTGTCTCTGAACAGTACACAAAGGTGT
  AATCTCTATGGTTGAAATGGACATACNAGCATGTATACCAAGAATTGTACTTTTACATGN
  TCCCTTAACAAANTNNGATTGCTTGAACTTTTAGTATGCACGACTGATNATAACTCTAAC
  CTGCACGGGAAGGTGGTTTTTTTTAATTTATACGAACACTGGCAAATGACTAGATTCTTT
  TTTTCAGCTGGGATAGCGNGACTAGTGACAACACCTAAACGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003017 unedited
  GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTTGCATTATGATTTA
  ATCTAATTGCACAAAAATTTGAAATTACAACACCTGCCATTTTCTAAGAGGAAGGTTATG
  CATGCCTCCATATTCAATTCTGCACTGTTACATTTGAACCATATTGTGAACTTTTACATC
  ATGATGGATCAGCTTAAAATTCCCATGCAACACCTCAAATTATAAAATGGAAGCAATTTT
  TAGACCTGTGGAAAGTTTTCATGCTAAAGATAAAAGCCATTTGTTAATGGGCAACTTACT
  TTGTGTTTAAGAACCAGGTTGCTGCTATACTTTAAAATGTTGGTCACTAAATATTATGGA
  CCCTGAACTGGCTTCTATGCCTAAAAAAAAAAAAAATGGGTTAACTATCACTTAGGTGAC
  ATCTATATTTCCTTCACTTCAGAAGACTAATCCATAGTGCCTCTCAGCTGTTTCTTATAA
  AATAAGAATCGTGTAACCAACTGCTTCCCATGGGATAAGAACAATGGTAACAGTGGGTTC
  AACTGACTATACACAATTATGCTAATATTTTATTAATTCAAAAGCACTTTACAAGAAAAT
  ATCAGTATTGGCTTCCAATAGGAATGTTATCCCTGGACTTGGTACCAAGCTCACAGATTT
  GAGTTTGCACGGAAAAACAAGCTTTTAAGTTAAAGACAATTTTGTACCCAAAACTTTAAT
  CCCAAGGATTTCTCCCAAAACCTTATTACAAATGACCGGCCTGGAAAAAAAATTCTTGGC
  CAGTAACCTCCAAGTTCGGCCTTAACATGTACCCTTAAACTGGCAGGACCTTTTTGAACA
  CACACAATTTGCCCTAAAGAATGGTCCAACCACCCTTGTATCCTATTACCGGCATTAAAT
  TAGGGACCTTAGGAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003017
• Insert Size: 2090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003017.3, NP_003008.1
• RefSeq Size: 2060 bp
• RefSeq ORF: 495 bp
• Locus ID: 6428
• Cytogenetics: 6p21.31-p21.2
• Domains: RRM
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Spliceosome
• Gene Summary: 'The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants, one protein-coding and the other non-coding, have been found for this gene. [provided by RefSeq, Sep 2010]'
  Transcript Variant: This variant (1) is the protein-coding variant. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.